FastQCFastQC Report
Fri 27 May 2016
SRR2138959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012401
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99200.049.751991
GTATCAA178250.035.063691
TCAACGC213050.028.8808884
ATCAACG214250.028.797543
CAACGCA219100.028.025895
TATCAAC224350.027.6153282
AACGCAG226200.027.2354816
CGCAGAG260400.023.6071438
ACGCAGA260550.023.561327
GTGGTAT48400.021.8695321
TGGTATC49650.019.8807542
GCAGAGT307650.019.8722849
GTACATG198400.019.180981
GAGTACT199050.019.1442612-13
TACATGG198750.018.5765912
AGAGTAC282550.018.11555910-11
CAGAGTA305000.017.95922310-11
ACATGGG201550.017.6920993
GTACTTT224350.016.95786714-15
AGTACTT214250.016.86491812-13
Produced by FastQC (version 0.11.5)