FastQCFastQC Report
Fri 27 May 2016
SRR2138958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5397608
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.050.554511
GTATCAA124500.035.4547041
TCAACGC154850.028.0919784
ATCAACG156050.027.903153
TATCAAC159900.027.3930972
CAACGCA160050.027.1527565
AACGCAG166450.026.1315676
TGGTATC29800.024.7823722
GTGGTAT32050.024.7641531
ACGCAGA188800.022.9899857
CGCAGAG194000.022.373768
GTACATG154700.020.7141231
TACATGG152450.020.713592
ACATGGG155650.019.6042143
GCAGAGT223450.019.4249679
GAGTACT140350.018.8344412-13
AGAGTAC203750.018.20039710-11
CAGAGTA218600.017.973310-11
CATGGGG120750.017.9247324
GTACTTT154450.017.19878414-15
Produced by FastQC (version 0.11.5)