##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5397608
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4481913099284	34.0	31.0	34.0	31.0	34.0
2	32.584044821335674	34.0	31.0	34.0	31.0	34.0
3	32.63019674641063	34.0	31.0	34.0	31.0	34.0
4	36.155840513056894	37.0	35.0	37.0	35.0	37.0
5	36.06525575773565	37.0	35.0	37.0	35.0	37.0
6	36.0816289363733	37.0	35.0	37.0	35.0	37.0
7	36.04025449791834	37.0	35.0	37.0	35.0	37.0
8	36.03727725318326	37.0	35.0	37.0	35.0	37.0
9	37.76586980751473	39.0	38.0	39.0	35.0	39.0
10-11	37.69559868000789	39.0	38.0	39.0	35.0	39.0
12-13	37.67162861771362	39.0	37.0	39.0	35.0	39.0
14-15	39.093066965959736	41.0	38.0	41.0	36.0	41.0
16-17	39.06733427103265	41.0	38.0	41.0	35.0	41.0
18-19	39.073651050613535	41.0	38.5	41.0	35.0	41.0
20-21	39.05780003420232	41.0	39.0	41.0	35.0	41.0
22-23	39.041867580281576	41.0	39.0	41.0	35.0	41.0
24-25	38.997845367766004	41.0	39.0	41.0	35.0	41.0
26-27	38.93462677622457	41.0	38.0	41.0	35.0	41.0
28-29	38.87553725047188	40.5	38.0	41.0	35.0	41.0
30-31	38.79242625404631	40.0	38.0	41.0	35.0	41.0
32-33	38.69466241413178	40.0	38.0	41.0	34.0	41.0
34-35	38.613742967187775	40.0	38.0	41.0	34.0	41.0
36-37	38.58963633661756	40.0	38.0	41.0	34.0	41.0
38-39	38.53369552533446	40.0	38.0	41.0	34.0	41.0
40-41	38.52207589108378	40.0	38.0	41.0	34.0	41.0
42-43	38.468942738803435	40.0	38.0	41.0	34.0	41.0
44-45	38.37813944964997	40.0	38.0	41.0	33.0	41.0
46-47	38.32374360649463	40.0	38.0	41.0	33.0	41.0
48-49	38.26521637491997	40.0	38.0	41.0	33.0	41.0
50-51	38.20345047680994	40.0	37.5	41.0	33.0	41.0
52-53	38.076476189368336	40.0	37.0	41.0	33.0	41.0
54-55	37.94852745961117	40.0	37.0	41.0	33.0	41.0
56-57	37.823142884694164	40.0	36.5	41.0	33.0	41.0
58-59	37.70599798848922	40.0	36.0	41.0	32.0	41.0
60-61	37.573754216890016	39.5	36.0	41.0	32.0	41.0
62-63	37.35652336221226	39.0	35.5	41.0	32.0	41.0
64-65	37.14651732094667	39.0	35.0	41.0	32.0	41.0
66-67	36.91550607149372	38.5	35.0	41.0	31.5	41.0
68-69	36.585157449231154	37.5	35.0	40.0	31.0	41.0
70-71	36.26017120597732	37.0	35.0	40.0	31.0	41.0
72-73	35.95524249407801	36.5	35.0	39.0	31.0	41.0
74-75	35.6874213200833	36.0	35.0	39.0	31.0	41.0
76-77	35.39785687796619	36.0	34.5	38.5	31.0	40.0
78-79	35.12909505019567	35.0	34.0	37.0	31.0	39.0
80-81	34.85308785605268	35.0	34.0	37.0	31.0	39.0
82-83	34.55723119849185	35.0	34.0	36.5	31.0	39.0
84-85	34.336376913735926	35.0	34.0	36.0	30.0	37.0
86-87	34.17849727604738	35.0	34.0	36.0	31.0	37.0
88-89	34.04084406932981	35.0	34.0	35.5	31.0	37.0
90-91	33.90917636849028	35.0	34.0	35.0	30.0	36.0
92-93	33.86552626888382	35.0	34.0	35.0	31.0	36.0
94-95	33.81101592666208	35.0	34.0	35.0	31.0	36.0
96-97	33.81075197024805	35.0	34.0	35.0	31.0	36.0
98-99	33.96393972600437	35.0	34.0	35.0	31.0	36.0
100	31.187128442140537	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	8.0
19	25.0
20	65.0
21	135.0
22	638.0
23	2140.0
24	5989.0
25	12890.0
26	23511.0
27	37043.0
28	54610.0
29	73576.0
30	94992.0
31	118823.0
32	147924.0
33	186085.0
34	244652.0
35	340353.0
36	495165.0
37	924806.0
38	1486773.0
39	1051525.0
40	95878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6327580661656	20.73027533677881	14.731080878789271	23.905885718266315
2	14.970994595415831	21.32907933084689	40.98898146655624	22.710944607181037
3	17.957866837507996	26.274802453904982	30.853799678783222	24.913531029803796
4	12.793778281045975	18.377251552910103	38.44267683018107	30.386293335862845
5	13.059376822775965	38.00330851208118	35.697615040092735	13.23969962505012
6	29.757233440672476	38.12279353030149	18.67032134851905	13.449651680506985
7	26.07508733498246	32.976459201927966	23.306972273644178	17.6414811894454
8	24.369479962235125	36.83757694148964	21.128951935746354	17.663991160528887
9	25.529660545930717	16.724260079650097	21.314626775416073	36.43145259900311
10-11	24.21835015545319	26.8060716923458	28.973903335469775	20.001674816731235
12-13	24.948282627520424	24.39711234943788	29.093824187982598	21.5607808350591
14-15	22.12506910468489	25.779669438758802	26.73418484632452	25.36107661023179
16-17	21.67893817914494	28.794383647831367	27.45504665410305	22.071631518920647
18-19	21.76288109335289	27.879865020540755	29.212512982713307	21.14474090339305
20-21	22.479923420565374	27.019013218067684	29.178048575099474	21.323014786267468
22-23	22.24182374730735	26.947706209253546	28.99911802402544	21.81135201941366
24-25	22.111165709991713	27.073082527371678	29.044610827891006	21.771140934745606
26-27	22.01084142060237	27.218148516947405	29.005765456386605	21.765244606063618
28-29	21.90306890655155	27.279429155786016	28.974578741098885	21.84292319656355
30-31	22.30174117898901	27.035955972339444	28.947319031813652	21.714983816857895
32-33	22.144113558238605	27.18765412507618	28.863072861023326	21.805159455661894
34-35	22.114741058930516	27.27241391711625	28.91649739590023	21.696347628053005
36-37	22.17524539866078	27.18887425384164	28.8288959309305	21.806984416567083
38-39	21.95586864656434	27.320557582346854	28.882221008107724	21.841352762981085
40-41	22.257435082591776	27.162498974628512	28.727472208634996	21.85259373414472
42-43	22.115668660327565	27.285114540378657	28.547648533664038	22.051568265629744
44-45	22.121349805969224	27.39307917533474	28.402947351250702	22.082623667445333
46-47	22.25757880217424	27.383458611480414	28.079994594775627	22.278967991569722
48-49	22.21567446526797	27.520782685406793	28.02870341832226	22.234839431002978
50-51	22.186142229650358	27.63603849017176	27.940654872186816	22.23716440799106
52-53	22.3120876531574	27.580582357545648	27.88429128260835	22.223038706688605
54-55	22.27206239779479	27.437474194158874	27.975935129269036	22.314528278777296
56-57	22.27948003337733	27.4970993413286	27.864033169837366	22.3593874554567
58-59	22.126901910003237	27.53898572551381	27.944371586639015	22.38974077784394
60-61	22.448204859082736	27.550613647258597	27.958413888940832	22.042767604717838
62-63	22.13927715324783	27.676594814766357	27.921663456274377	22.262464575711437
64-65	22.013747620634298	27.64772990596569	27.92065276351655	22.417869709883462
66-67	22.1522965434809	27.727019588663737	27.898866654600923	22.221817213254443
68-69	22.105696373142735	27.845829874798827	27.792717460639434	22.25575629141901
70-71	22.15316910335958	27.54601233612876	27.64298375933652	22.657834801175134
72-73	22.216468760921735	27.551824211820016	27.53541776270104	22.69628926455721
74-75	22.080003012400386	27.68492846319796	27.789745903586894	22.44532262081476
76-77	22.277371892519398	27.420192358391482	27.637341404219374	22.66509434486975
78-79	22.200800573672872	27.47514779531879	27.406047516927316	22.91800411408102
80-81	22.436579411109722	27.70781910696295	27.38771070343779	22.467890778489537
82-83	22.435422791977427	27.539634950269647	27.522067225367103	22.50287503238582
84-85	22.350421368985852	27.504255109476354	27.44866439766277	22.696659123875023
86-87	22.167690682816456	27.80571381354804	27.493182805568452	22.533412698067057
88-89	22.439880396664293	27.719182695442125	27.485060607978458	22.355876299915124
90-91	22.509557898562317	27.7148491396079	27.488847306076508	22.286745655753272
92-93	22.408861612081388	27.418977033916846	27.545596007028394	22.626565346973372
94-95	22.39195591702982	27.763673913111198	27.64455769069031	22.199812479168678
96-97	22.56533015130377	27.701762816136995	27.724181119475904	22.00872591308333
98-99	23.2313089585839	29.6572855407095	28.09366813883234	19.01773736187426
100	22.352817641094493	30.650858446792927	21.52810296809272	25.468220944019855
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1712.0
1	1320.5
2	1382.0
3	1883.0
4	2273.0
5	3595.5
6	4949.0
7	6285.0
8	7196.5
9	7116.0
10	6180.5
11	4864.5
12	4861.5
13	6389.0
14	10912.5
15	17761.0
16	23399.0
17	26430.0
18	27329.5
19	26596.0
20	24792.5
21	22825.5
22	21997.5
23	23672.5
24	28185.0
25	35470.5
26	44975.0
27	54814.5
28	64773.0
29	76535.5
30	88049.0
31	99533.5
32	111643.5
33	123768.0
34	136422.5
35	148204.5
36	160651.5
37	172345.0
38	181154.5
39	188901.5
40	197311.5
41	204625.5
42	209240.0
43	212962.5
44	216728.5
45	220828.0
46	224468.5
47	226774.5
48	227448.0
49	226077.5
50	222299.5
51	216112.0
52	206676.0
53	194289.5
54	181728.5
55	169173.0
56	157385.5
57	144128.0
58	128588.5
59	112485.0
60	94515.0
61	77115.5
62	61949.5
63	48962.5
64	38092.5
65	29635.0
66	23251.0
67	18185.0
68	13931.5
69	10721.0
70	8370.0
71	6289.5
72	4788.0
73	3637.0
74	2717.0
75	2054.0
76	1541.0
77	1152.5
78	889.0
79	696.0
80	535.5
81	389.0
82	299.5
83	216.5
84	145.5
85	112.5
86	88.5
87	64.5
88	43.5
89	32.5
90	27.0
91	20.5
92	14.5
93	7.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006262033107998951
3	1.296870762011617E-4
4	0.0
5	0.011486569606388608
6	0.012876073994258198
7	0.0
8	0.0
9	0.0
10-11	4.631681292898632E-5
12-13	3.7053450343189056E-5
14-15	0.0
16-17	2.2232070205913435E-4
18-19	8.522293578933482E-4
20-21	0.0
22-23	1.1118499766001908E-4
24-25	0.0
26-27	9.272352163935187E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.670609906924086E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.9120173733546612E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.006512412688320984
52-53	6.988924651105394E-5
54-55	2.0111708473545463E-5
56-57	0.0
58-59	0.0
60-61	3.082156420465724E-5
62-63	0.0010262921536543879
64-65	0.0013599926769625088
66-67	9.72012074503035E-4
68-69	0.009831502353902915
70-71	7.552234490407583E-4
72-73	4.905558728422492E-4
74-75	3.963669009855663E-4
76-77	1.2236240875518608E-4
78-79	0.0
80-81	1.0236179366523804E-4
82-83	0.0
84-85	1.1634722759681776E-5
86-87	1.5301660465570676E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	196.0
20-21	691.0
22-23	1336.0
24-25	2303.0
26-27	3309.0
28-29	5352.0
30-31	11951.0
32-33	15231.0
34-35	20419.0
36-37	29428.0
38-39	32287.0
40-41	35493.0
42-43	41261.0
44-45	49808.0
46-47	49751.0
48-49	44393.0
50-51	37799.0
52-53	35167.0
54-55	36318.0
56-57	40978.0
58-59	26127.0
60-61	44071.0
62-63	43226.0
64-65	46181.0
66-67	48437.0
68-69	49318.0
70-71	48786.0
72-73	45421.0
74-75	45834.0
76-77	47809.0
78-79	50038.0
80-81	50527.0
82-83	48867.0
84-85	48899.0
86-87	51835.0
88-89	55682.0
90-91	58705.0
92-93	63177.0
94-95	79989.0
96-97	216220.0
98-99	439909.0
100-101	3295079.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.72639261452915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.69179963239017	78.65372621836879
2	6.683266774098717	11.59231236396246
3	1.5016988993340985	3.907107849973659
4	0.5559116595219918	1.9284885137079484
5	0.22730424026049945	0.9856638391889667
6	0.12165218811523648	0.6330273257339133
7	0.06545770691080799	0.39738375524354574
8	0.040443963233085765	0.28060472273921416
9	0.026660887467668544	0.20809823345755216
>10	0.0828745812134004	1.1008212500564953
>50	0.0019845976663426853	0.11436312378957991
>100	9.127375005491694E-4	0.17761389211359224
>500	3.213228749928254E-5	0.020788911664242158
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.8526725171594528E-5	0.0
3	0.0	0.0	0.0	1.8526725171594528E-5	0.0
4	0.0	3.7053450343189056E-5	0.0	1.8526725171594528E-5	0.0
5	0.0	3.7053450343189056E-5	0.0	3.7053450343189056E-5	0.0
6	0.0	3.7053450343189056E-5	0.0	5.558017551478359E-5	1.8526725171594528E-5
7	0.0	3.7053450343189056E-5	0.0	5.558017551478359E-5	1.8526725171594528E-5
8	0.0	5.558017551478359E-5	0.0	5.558017551478359E-5	1.8526725171594528E-5
9	0.0	9.263362585797264E-5	0.0	5.558017551478359E-5	1.8526725171594528E-5
10-11	0.0	9.263362585797264E-5	0.0	1.1116035102956718E-4	1.8526725171594528E-5
12-13	0.0	9.263362585797264E-5	0.0	1.1116035102956718E-4	1.8526725171594528E-5
14-15	0.0	1.6674052654435076E-4	0.0	1.1116035102956718E-4	1.8526725171594528E-5
16-17	0.0	1.6674052654435076E-4	0.0	1.1116035102956718E-4	1.8526725171594528E-5
18-19	0.0	2.0379397688753982E-4	0.0	1.2042371361536443E-4	1.8526725171594528E-5
20-21	0.0	2.4084742723072888E-4	0.0	1.296870762011617E-4	1.8526725171594528E-5
22-23	0.0	2.8716424015971516E-4	0.0	1.3895043878695896E-4	4.631681292898632E-5
24-25	0.0	2.9642760274551245E-4	0.0	1.6674052654435076E-4	7.410690068637811E-5
26-27	0.0	2.9642760274551245E-4	0.0	2.686375149881207E-4	7.410690068637811E-5
28-29	0.0	2.9642760274551245E-4	0.0	8.892828082365373E-4	7.410690068637811E-5
30-31	0.0	2.9642760274551245E-4	0.0	0.0031032264662420837	8.337026327217538E-5
32-33	0.0	3.14954327917107E-4	0.0	0.00863345392996305	1.1116035102956718E-4
34-35	0.0	3.705345034318906E-4	0.0	0.016470258677547533	1.1116035102956718E-4
36-37	0.0	4.53904766704066E-4	0.0	0.029457493022835304	1.1116035102956718E-4
38-39	0.0	4.8169485446145777E-4	0.0	0.06627935930137943	1.1116035102956718E-4
40-41	0.0	5.187483048046468E-4	0.0	0.1039441915752311	1.2042371361536443E-4
42-43	0.0	5.558017551478358E-4	0.0	0.12675985362404976	1.296870762011617E-4
44-45	0.0	6.29908655834214E-4	0.0	0.15708810272995	1.296870762011617E-4
46-47	0.0	6.66962106177403E-4	0.0	0.1882407911059862	1.4821380137275623E-4
48-49	0.0	6.66962106177403E-4	0.0	0.21222363684061532	1.4821380137275623E-4
50-51	0.0	7.318056442779839E-4	0.0	0.23676228433039226	1.4821380137275623E-4
52-53	0.0	7.410690068637812E-4	0.0	0.31791860394456206	1.6674052654435076E-4
54-55	0.0	7.595957320353757E-4	0.0	0.4153969684349067	1.852672517159453E-4
56-57	0.0	7.781224572069702E-4	0.0	0.5274373389101246	1.852672517159453E-4
58-59	0.0	7.781224572069702E-4	0.0	0.7444223441198398	1.852672517159453E-4
60-61	0.0	8.05912544964362E-4	0.0	0.9198426414070826	1.852672517159453E-4
62-63	0.0	8.244392701359565E-4	0.0	0.925854563725265	1.852672517159453E-4
64-65	0.0	9.633897089229155E-4	0.0	0.9314311080019149	1.852672517159453E-4
66-67	0.0	9.819164340945101E-4	0.0	0.9367760682139199	1.852672517159453E-4
68-69	0.0	0.001018969884437699	0.0	0.9422229254143687	1.852672517159453E-4
70-71	0.0	0.001046759972195091	0.0	0.9468638700698532	2.2232070205913435E-4
72-73	0.0	0.0010745500599524827	0.0	0.950893432794675	2.4084742723072888E-4
74-75	0.0	0.0010838134225382798	0.0	0.9536261247574851	2.4084742723072888E-4
76-77	0.0	0.0011116035102956717	0.0	0.9567478779488989	2.5011078981652615E-4
78-79	0.0	0.0011301302354672662	0.0	0.9588599246184606	2.593741524023234E-4
80-81	0.0	0.0011301302354672662	0.0	0.9596380470756676	2.593741524023234E-4
82-83	0.0	0.0011486569606388606	0.0	0.9605921734220046	2.8716424015971516E-4
84-85	0.0	0.0011857104109820498	0.0	0.9608608109369928	2.9642760274551245E-4
86-87	0.0	0.0012042371361536443	0.0	0.9609071277499218	2.9642760274551245E-4
88	0.0	0.0012783440368400224	0.0	0.9609256544750934	3.14954327917107E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7195	0.0	50.55451	1
GTATCAA	12450	0.0	35.454704	1
TCAACGC	15485	0.0	28.091978	4
ATCAACG	15605	0.0	27.90315	3
TATCAAC	15990	0.0	27.393097	2
CAACGCA	16005	0.0	27.152756	5
AACGCAG	16645	0.0	26.131567	6
TGGTATC	2980	0.0	24.782372	2
GTGGTAT	3205	0.0	24.764153	1
ACGCAGA	18880	0.0	22.989985	7
CGCAGAG	19400	0.0	22.37376	8
GTACATG	15470	0.0	20.714123	1
TACATGG	15245	0.0	20.71359	2
ACATGGG	15565	0.0	19.604214	3
GCAGAGT	22345	0.0	19.424967	9
GAGTACT	14035	0.0	18.83444	12-13
AGAGTAC	20375	0.0	18.200397	10-11
CAGAGTA	21860	0.0	17.9733	10-11
CATGGGG	12075	0.0	17.924732	4
GTACTTT	15445	0.0	17.198784	14-15
>>END_MODULE