FastQCFastQC Report
Fri 27 May 2016
SRR2138957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9593184
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111450.049.574921
GTATCAA193750.031.8960551
ATCTCGT131500.031.51314794
TCAACGC219950.027.095754
ATCAACG221100.026.974063
TATCTCG156100.026.91374894
CAACGCA227200.026.249715
TATCAAC236300.025.4018142
AACGCAG235650.025.2876176
ACGCAGA271450.021.93377
CGCAGAG277100.021.5321948
CCGTCTT85350.020.97374290-91
GCCGTCT89600.020.77672290-91
ATGCCGT88950.020.4170788-89
TATGCCG86750.020.2623986-87
GTACATG237050.020.1538031
TGCCGTC87250.020.14880488-89
CTATCTC220150.020.09133594
TACATGG231350.019.9747872
CGCTATC219750.019.8673594
Produced by FastQC (version 0.11.5)