##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9593184
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51231843358785	34.0	31.0	34.0	31.0	34.0
2	32.64498210396048	34.0	31.0	34.0	31.0	34.0
3	32.693996487506126	34.0	31.0	34.0	31.0	34.0
4	36.19894260341509	37.0	35.0	37.0	35.0	37.0
5	36.112944878363635	37.0	35.0	37.0	35.0	37.0
6	36.12542790798133	37.0	36.0	37.0	35.0	37.0
7	36.086109054095076	37.0	36.0	37.0	35.0	37.0
8	36.08277710507794	37.0	36.0	37.0	35.0	37.0
9	37.82760520386141	39.0	38.0	39.0	35.0	39.0
10-11	37.76127060629714	39.0	38.0	39.0	35.0	39.0
12-13	37.74203027899809	39.0	38.0	39.0	35.0	39.0
14-15	39.186837915336554	41.0	39.0	41.0	36.0	41.0
16-17	39.1607193711702	41.0	39.0	41.0	36.0	41.0
18-19	39.16223049615227	41.0	39.0	41.0	36.0	41.0
20-21	39.14702497999444	41.0	39.0	41.0	35.5	41.0
22-23	39.13197562259212	41.0	39.0	41.0	35.0	41.0
24-25	39.089774228999914	41.0	39.0	41.0	35.0	41.0
26-27	39.03365338752938	41.0	39.0	41.0	35.0	41.0
28-29	38.98090014252322	41.0	39.0	41.0	35.0	41.0
30-31	38.90529470511057	40.5	38.0	41.0	35.0	41.0
32-33	38.819157596685116	40.0	38.0	41.0	35.0	41.0
34-35	38.74428696379263	40.0	38.0	41.0	34.5	41.0
36-37	38.72015960526124	40.0	38.0	41.0	34.0	41.0
38-39	38.67391723379025	40.0	38.0	41.0	34.0	41.0
40-41	38.66279490713055	40.0	38.0	41.0	34.0	41.0
42-43	38.612779442534304	40.0	38.0	41.0	34.0	41.0
44-45	38.52880373655253	40.0	38.0	41.0	34.0	41.0
46-47	38.480427441152855	40.0	38.0	41.0	34.0	41.0
48-49	38.428860226760065	40.0	38.0	41.0	33.0	41.0
50-51	38.359906943520926	40.0	38.0	41.0	33.0	41.0
52-53	38.2287962099617	40.0	37.5	41.0	33.0	41.0
54-55	38.11357840007169	40.0	37.0	41.0	33.0	41.0
56-57	37.990490430416884	40.0	37.0	41.0	33.0	41.0
58-59	37.882947324932225	40.0	36.5	41.0	33.0	41.0
60-61	37.75039365717316	40.0	36.0	41.0	33.0	41.0
62-63	37.54512683015248	39.0	36.0	41.0	32.0	41.0
64-65	37.345891259566315	39.0	35.0	41.0	32.0	41.0
66-67	37.11857877638435	39.0	35.0	41.0	32.0	41.0
68-69	36.78453794799888	38.0	35.0	40.5	31.5	41.0
70-71	36.454032659523705	37.0	35.0	40.0	31.0	41.0
72-73	36.15133460595925	37.0	35.0	39.5	31.0	41.0
74-75	35.86661572003837	36.0	35.0	39.0	31.0	41.0
76-77	35.5574302250825	36.0	35.0	39.0	31.0	40.5
78-79	35.26334653776389	35.5	34.5	37.5	31.0	39.5
80-81	34.953668311850194	35.0	34.0	37.0	31.0	39.0
82-83	34.63762156249982	35.0	34.0	36.5	31.0	39.0
84-85	34.417947967667374	35.0	34.0	36.0	31.0	37.5
86-87	34.246760567817695	35.0	34.0	36.0	31.0	37.0
88-89	34.09639980492986	35.0	34.0	35.5	31.0	37.0
90-91	33.96958385319762	35.0	34.0	35.0	31.0	36.0
92-93	33.910411183139516	35.0	34.0	35.0	31.0	36.0
94-95	33.83044418917793	35.0	34.0	35.0	31.0	36.0
96-97	33.81743744479123	35.0	34.0	35.0	31.0	36.0
98-99	33.97124397704502	35.0	34.0	35.0	31.0	36.0
100	31.082078387946595	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	25.0
19	50.0
20	95.0
21	285.0
22	1065.0
23	3510.0
24	9465.0
25	20541.0
26	37072.0
27	60576.0
28	88566.0
29	121578.0
30	157991.0
31	199125.0
32	248764.0
33	313400.0
34	412364.0
35	567292.0
36	841642.0
37	1587760.0
38	2701649.0
39	2009201.0
40	211167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55866123280863	20.711371740602495	14.275875454906316	24.45409157168256
2	14.63234691624514	21.401483284743257	41.26032291497915	22.70584688403245
3	17.840175033099115	26.211027297895782	30.949540385923008	24.999257283082095
4	12.533555074102612	18.480402335658315	38.81415179777642	30.171890792462648
5	12.962166007684269	37.92261768116178	36.037465145658985	13.07775116549497
6	29.494034149877436	38.427895881270366	18.850113912504895	13.22795605634731
7	25.820499221113657	32.86466724707876	23.59070773582577	17.724125795981813
8	23.91147714877563	37.17167313792793	21.483586679876048	17.433263033420396
9	25.10632549109868	16.759242812396803	21.619235073568902	36.51519662293562
10-11	23.806263804730424	26.841086776343897	29.48307333783723	19.86957608108845
12-13	24.5666908001347	24.46035482400333	29.434952138126064	21.538002237735906
14-15	21.81162507853885	25.926172049786402	26.843271250182095	25.418931621492657
16-17	21.50415801423535	29.03666997770599	27.597389431908837	21.86178257614982
18-19	21.564053184298352	28.17694540588545	29.170717540622583	21.088283869193617
20-21	22.239335433134563	27.3231370588369	29.06473536937744	21.372792138651096
22-23	21.988496256977538	27.358344994058687	28.890027882522446	21.76313086644133
24-25	21.934019253987504	27.43477863212236	28.932890954593017	21.698311159297123
26-27	21.861862024788373	27.563105189043902	28.88074943448784	21.694283351679886
28-29	21.79962809010832	27.536044237417133	28.92685337062526	21.737474301849286
30-31	21.959211279884904	27.30369326370421	28.965385823754307	21.77170963265658
32-33	21.714482070285825	27.583485282434395	28.829628768614835	21.87240387866494
34-35	21.95966896961981	27.52042872086885	28.81281178655884	21.707090522952505
36-37	21.893072441399955	27.47938585624004	28.86527672832848	21.762264974031524
38-39	21.711362803889944	27.535881456366184	28.817832343885808	21.934923395858064
40-41	22.071565923711294	27.338869335738686	28.796841643502592	21.792723097047425
42-43	21.915589444931026	27.4011601083754	28.638406829049543	22.044843617644034
44-45	21.884214519921056	27.533706304337002	28.504067603069387	22.07801157267256
46-47	22.024129837377476	27.441335482159126	28.38502599381582	22.149508686647582
48-49	21.94728436564875	27.73996250970907	28.143460181792797	22.169292942849385
50-51	21.81183647627577	27.79501680878364	28.06547206338033	22.327674651560255
52-53	21.82275276145937	27.625826853285997	28.075475456555438	22.475944928699203
54-55	22.025346269273168	27.28939182056223	28.36205526661844	22.323206643546158
56-57	21.897545433717642	27.003639873151243	28.403762649420404	22.69505204371071
58-59	21.549897378112497	26.92065119719474	28.59731826014927	22.932133164543494
60-61	21.855532955504486	27.29309148986596	28.731122883308096	22.120252671321452
62-63	21.2730516749738	27.659826471650856	28.66402075492002	22.403101098455323
64-65	21.049715788734645	28.06837817439709	28.23445524851746	22.647450788350806
66-67	21.177800602030832	28.686591330827145	27.70784376594644	22.427764301195584
68-69	21.287615767489097	28.640339256084296	27.499024853656017	22.573020122770597
70-71	21.309011058017273	27.95592090730816	27.479426163379163	23.255641871295406
72-73	21.63757459792783	27.28864110197972	27.470942701677338	23.602841598415107
74-75	21.737099358201185	27.33660805636649	27.72913282300279	23.197159762429543
76-77	22.133549037466306	27.24550132244176	26.96286539172367	23.65808424836826
78-79	22.364507279181982	27.32923931862287	26.43855819037998	23.867695211815164
80-81	22.176948741236806	27.779844284197342	26.624600024323925	23.418606950241923
82-83	22.079861876346467	27.54800771802733	26.733070824666978	23.63905958095923
84-85	22.23216644761717	28.011144080808787	26.662746938400865	23.093942533173173
86-87	22.817607709106916	27.846703044355724	26.730895323249065	22.604793923288295
88-89	22.411723292501375	28.016240531485476	27.19475004841127	22.377286127601874
90-91	22.353565987974207	27.57197683240349	27.032282651716162	23.04217452790615
92-93	22.411125106901643	26.991447235067863	27.320577912014077	23.276849746016413
94-95	21.55885237893341	27.582146682911013	28.066265356508442	22.792735581647136
96-97	21.679607406474013	27.441626988217106	28.252727377255344	22.62603822805354
98-99	22.79664865153633	29.226309914743133	28.655664405399524	19.321377028321017
100	21.96155897628509	30.356946815919887	21.829642774313864	25.85185143348116
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1720.0
1	1457.5
2	1921.5
3	2722.5
4	3440.0
5	5691.0
6	7730.0
7	9177.5
8	10108.0
9	9819.0
10	8638.5
11	7123.5
12	7046.5
13	9117.5
14	15755.0
15	25960.5
16	34546.5
17	39330.0
18	41393.5
19	41478.0
20	39589.0
21	37548.5
22	37639.5
23	42254.0
24	51723.5
25	65619.5
26	84360.5
27	104822.0
28	125084.5
29	147704.5
30	169614.0
31	190399.5
32	212372.0
33	233585.0
34	253713.0
35	272012.0
36	292713.0
37	314820.0
38	332194.0
39	345405.5
40	358798.0
41	372076.0
42	382244.0
43	391088.0
44	398998.5
45	406761.5
46	414129.0
47	416383.0
48	413504.0
49	405270.5
50	394576.5
51	382171.0
52	364961.5
53	344624.0
54	322879.0
55	299225.0
56	273744.0
57	246150.0
58	218182.5
59	190523.5
60	158793.0
61	127707.0
62	102030.5
63	80194.0
64	61521.5
65	46504.5
66	35220.5
67	27746.5
68	22039.0
69	16862.5
70	12872.0
71	9928.5
72	7560.0
73	5627.0
74	4211.5
75	3200.5
76	2408.5
77	1807.5
78	1361.0
79	1042.0
80	780.5
81	543.0
82	397.5
83	284.0
84	206.0
85	157.5
86	118.0
87	95.0
88	71.0
89	54.0
90	37.5
91	25.5
92	20.0
93	14.0
94	9.0
95	5.5
96	4.0
97	3.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007192606750793063
3	2.8144982937885896E-4
4	0.0
5	0.011695804020854806
6	0.012227431476348208
7	0.0
8	0.0
9	0.0
10-11	5.212033877386277E-5
12-13	5.733237265124906E-5
14-15	1.5636101632158832E-5
16-17	2.3975355835976876E-4
18-19	9.329540640521436E-4
20-21	0.0
22-23	1.4596520377211622E-4
24-25	0.0
26-27	5.216781258483791E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.686352685263052E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.1627063458399937E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0064453060240322515
52-53	1.0752122879334752E-4
54-55	6.800589928507665E-5
56-57	0.0
58-59	1.1365807491180985E-5
60-61	8.022324064989535E-5
62-63	0.0010425075503609335
64-65	0.001428750691611951
66-67	0.0010904948321805497
68-69	0.01038367499025255
70-71	8.451327648852808E-4
72-73	5.723239286973003E-4
74-75	3.9841855226028134E-4
76-77	6.931361936375517E-5
78-79	0.0
80-81	2.0690165737926076E-4
82-83	0.0
84-85	6.6368574426913995E-6
86-87	2.219678037718518E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	303.0
20-21	1089.0
22-23	2247.0
24-25	3809.0
26-27	6256.0
28-29	11330.0
30-31	27907.0
32-33	35025.0
34-35	47759.0
36-37	67620.0
38-39	44695.0
40-41	77205.0
42-43	86964.0
44-45	100670.0
46-47	100870.0
48-49	91164.0
50-51	49528.0
52-53	12799.0
54-55	12596.0
56-57	12067.0
58-59	51954.0
60-61	93311.0
62-63	92746.0
64-65	100327.0
66-67	105861.0
68-69	107462.0
70-71	101741.0
72-73	92703.0
74-75	95744.0
76-77	99085.0
78-79	101435.0
80-81	102216.0
82-83	98626.0
84-85	98758.0
86-87	104492.0
88-89	109318.0
90-91	113989.0
92-93	122238.0
94-95	149807.0
96-97	374965.0
98-99	818785.0
100-101	5665718.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.80808407312749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.17429169011479	73.01544159363492
2	8.032092756198294	13.30244424476853
3	2.08887335715571	5.1892680173239825
4	0.7737104008748311	2.562779036955847
5	0.34513954422055865	1.4290172197388458
6	0.19560325281529015	0.9718518362463455
7	0.11173943436455792	0.6477049932600817
8	0.07058981589850265	0.4676325927703838
9	0.05443942788913215	0.40572222493825943
>10	0.15133164979235436	1.8433739265749607
>50	0.001723148696906389	0.0926903936752633
>100	4.441851702835347E-4	0.05857510773618018
>500	2.1336808820886372E-5	0.013498812376408462
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0424067754772555E-5	0.0	0.0	0.0
3	0.0	1.0424067754772555E-5	0.0	0.0	0.0
4	0.0	1.0424067754772555E-5	0.0	1.0424067754772555E-5	0.0
5	0.0	1.0424067754772555E-5	0.0	1.0424067754772555E-5	0.0
6	0.0	1.0424067754772555E-5	0.0	1.0424067754772555E-5	1.0424067754772555E-5
7	0.0	1.0424067754772555E-5	0.0	1.0424067754772555E-5	2.084813550954511E-5
8	0.0	3.1272203264317665E-5	0.0	1.0424067754772555E-5	2.084813550954511E-5
9	0.0	7.296847428340789E-5	0.0	2.084813550954511E-5	3.1272203264317665E-5
10-11	0.0	7.296847428340789E-5	0.0	4.169627101909022E-5	3.1272203264317665E-5
12-13	0.0	7.296847428340789E-5	0.0	6.775644040602161E-5	3.1272203264317665E-5
14-15	0.0	1.3030084693465692E-4	0.0	7.818050816079416E-5	4.169627101909022E-5
16-17	0.0	1.3551288081204322E-4	0.0	8.339254203818044E-5	5.212033877386278E-5
18-19	0.0	1.4593694856681577E-4	0.0	9.3816609792953E-5	6.254440652863533E-5
20-21	0.0	1.5636101632158833E-4	0.0	1.2508881305727066E-4	6.254440652863533E-5
22-23	0.0	1.6157305019897462E-4	0.0	1.5114898244420204E-4	9.381660979295299E-5
24-25	0.0	2.084813550954511E-4	0.0	2.1369338897283738E-4	1.2508881305727066E-4
26-27	0.0	2.1890542285022365E-4	0.0	3.8569050692658453E-4	1.2508881305727066E-4
28-29	0.0	2.1890542285022365E-4	0.0	0.0015688221970932693	1.2508881305727066E-4
30-31	0.0	2.81449829378859E-4	0.0	0.005847902010427403	1.3030084693465692E-4
32-33	0.0	2.81449829378859E-4	0.0	0.01501065756687248	1.4593694856681577E-4
34-35	0.0	3.3878220203010807E-4	0.0	0.027800988701978405	1.5636101632158833E-4
36-37	0.0	4.0132660855874336E-4	0.0	0.0511613245404237	1.5636101632158833E-4
38-39	0.0	4.2738677794567476E-4	0.0	0.24991702442067198	1.5636101632158833E-4
40-41	0.0	4.69083048964765E-4	0.0	0.4565585315574058	1.5636101632158833E-4
42-43	0.0	5.05567286106469E-4	0.0	0.5057236471227905	1.5636101632158833E-4
44-45	0.0	5.889598281446494E-4	0.0	0.5792185368278144	1.5636101632158833E-4
46-47	0.0	6.358681330411259E-4	0.0	0.6569768702445403	1.7199711795374715E-4
48-49	0.0	6.358681330411259E-4	0.0	0.7176814288144583	1.9805728734067856E-4
50-51	0.0	6.775644040602161E-4	0.0	0.7762542655285252	1.9805728734067856E-4
52-53	0.0	6.879884718149886E-4	0.0	1.3817518771661215	2.2411745672760994E-4
54-55	0.0	6.879884718149886E-4	0.0	2.1928590132327286	2.553896599919276E-4
56-57	0.0	6.879884718149886E-4	0.0	3.0619552382191353	2.7102576162408643E-4
58-59	0.0	6.879884718149886E-4	0.0	3.9422260638386586	2.81449829378859E-4
60-61	0.0	7.036245734471475E-4	0.0	4.179227668311167	2.81449829378859E-4
62-63	0.0	7.348967767114652E-4	0.0	4.188260123020678	2.81449829378859E-4
64-65	0.0	8.130772848722593E-4	0.0	4.196083385870635	2.81449829378859E-4
66-67	0.0	8.182893187496456E-4	0.0	4.20295284652103	2.81449829378859E-4
68-69	0.0	8.65197623646122E-4	0.0	4.209796247002038	2.81449829378859E-4
70-71	0.0	8.860457591556672E-4	0.0	4.215883902570825	2.81449829378859E-4
72-73	0.0	8.860457591556672E-4	0.0	4.220809274584955	3.231461003979492E-4
74-75	0.0	9.173179624199848E-4	0.0	4.224687027789731	3.231461003979492E-4
76-77	0.0	9.329540640521437E-4	0.0	4.228371935741043	3.231461003979492E-4
78-79	0.0	9.485901656843026E-4	0.0	4.23091019623933	3.231461003979492E-4
80-81	0.0	9.485901656843026E-4	0.0	4.231744121659712	3.335701681527217E-4
82-83	0.0	9.485901656843026E-4	0.0	4.232682287757641	3.544183036622669E-4
84-85	0.0	9.59014233439075E-4	0.0	4.23310446250171	3.596303375396531E-4
86-87	0.0	9.694383011938476E-4	0.0	4.233234763348644	3.648423714170394E-4
88	0.0	0.001052830843232028	0.0	4.233234763348644	3.648423714170394E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11145	0.0	49.57492	1
GTATCAA	19375	0.0	31.896055	1
ATCTCGT	13150	0.0	31.513147	94
TCAACGC	21995	0.0	27.09575	4
ATCAACG	22110	0.0	26.97406	3
TATCTCG	15610	0.0	26.913748	94
CAACGCA	22720	0.0	26.24971	5
TATCAAC	23630	0.0	25.401814	2
AACGCAG	23565	0.0	25.287617	6
ACGCAGA	27145	0.0	21.9337	7
CGCAGAG	27710	0.0	21.532194	8
CCGTCTT	8535	0.0	20.973742	90-91
GCCGTCT	8960	0.0	20.776722	90-91
ATGCCGT	8895	0.0	20.41707	88-89
TATGCCG	8675	0.0	20.26239	86-87
GTACATG	23705	0.0	20.153803	1
TGCCGTC	8725	0.0	20.148804	88-89
CTATCTC	22015	0.0	20.091335	94
TACATGG	23135	0.0	19.974787	2
CGCTATC	21975	0.0	19.86735	94
>>END_MODULE