FastQCFastQC Report
Fri 27 May 2016
SRR2138956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8761704
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112750.052.0551871
CTTATAC158900.047.261791
TATACAC207550.038.421373
GTATCAA216800.038.287071
TTATACA203050.037.985072
ATCAACG259800.031.3289243
TCAACGC262150.031.0806944
CAACGCA268750.030.3476665
TATCAAC271450.030.3296382
AACGCAG280000.029.1421076
TACACAT277400.028.532575
ACATCTC274850.027.487688
ACACATC280700.027.0812666
ATACACA321800.025.9370144
CACATCT293000.025.8138057
CGCAGAG319100.025.5175388
ACGCAGA319550.025.4948357
CATCTCC302500.024.8633619
TGGTATC47750.023.9986612
GTGGTAT54550.022.9451
Produced by FastQC (version 0.11.5)