FastQCFastQC Report
Fri 27 May 2016
SRR2138955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9982213
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC265200.056.5638851
GGTATCA124000.050.790761
TTATACA316550.047.6920852
TATACAC324750.047.4386443
GTATCAA228600.037.1731071
TACACAT454400.033.9857065
ATACACA475300.032.9494364
ACACATC505650.030.1089446
ATCAACG275450.030.0748543
TCAACGC276750.029.9941734
CAACGCA280450.029.5680435
ACATCTC514000.029.5526668
CACATCT524050.028.922097
TATCAAC290000.028.8412062
AACGCAG290650.028.5859346
CATCTCC558350.027.0555159
CGCAGAG339350.024.4079328
ACGCAGA340800.024.2918157
GTGGTAT56500.021.539021
GCAGAGT391100.021.1676069
Produced by FastQC (version 0.11.5)