##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9982213
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.509656826597467	33.0	31.0	34.0	28.0	34.0
2	32.159981659377536	33.0	31.0	34.0	30.0	34.0
3	32.225497091676964	33.0	31.0	34.0	30.0	34.0
4	33.93358356508722	34.0	33.0	37.0	31.0	37.0
5	34.201565023707666	34.0	33.0	37.0	33.0	37.0
6	36.35285572447713	37.0	37.0	38.0	34.0	38.0
7	36.69508114082519	37.0	37.0	38.0	35.0	38.0
8	36.67822465819954	37.0	37.0	38.0	35.0	38.0
9	37.508137023323386	38.0	38.0	39.0	36.0	39.0
10-11	37.51179708347237	38.0	38.0	39.0	36.0	39.0
12-13	37.52035515571547	38.0	38.0	39.0	36.0	39.0
14-15	38.139787289652105	38.0	38.0	40.0	36.0	41.0
16-17	38.08732492484382	38.0	38.0	40.0	36.0	41.0
18-19	38.11685489981029	38.0	38.0	40.0	36.0	41.0
20-21	38.132543203779726	38.0	38.0	40.0	36.0	41.0
22-23	38.13439115000263	38.0	38.0	40.0	36.0	41.0
24-25	38.11158704369807	38.0	38.0	40.0	36.0	41.0
26-27	38.056319303535155	38.0	38.0	40.0	36.0	41.0
28-29	38.011957821472045	38.0	38.0	40.0	36.0	41.0
30-31	37.90587128509905	38.0	38.0	40.0	36.0	41.0
32-33	37.88543035116433	38.0	38.0	40.0	36.0	41.0
34-35	37.82661976265369	38.0	38.0	40.0	35.0	41.0
36-37	37.78622610677412	38.0	38.0	40.0	35.0	41.0
38-39	37.67599551390654	38.0	38.0	40.0	35.0	41.0
40-41	37.76391609414329	38.0	38.0	40.0	35.0	41.0
42-43	37.74965455139275	38.0	38.0	40.0	35.0	41.0
44-45	37.68887708326246	38.0	38.0	40.0	35.0	41.0
46-47	37.640359008807856	38.0	38.0	40.0	35.0	41.0
48-49	37.605336490870286	38.0	38.0	40.0	35.0	41.0
50-51	37.530295522567926	38.0	38.0	39.0	35.0	41.0
52-53	37.464057704459336	38.0	38.0	39.0	35.0	41.0
54-55	37.39885599137433	38.0	38.0	39.0	34.0	41.0
56-57	37.33684976685268	38.0	38.0	39.0	34.0	41.0
58-59	37.28168031156968	38.0	38.0	38.0	34.0	41.0
60-61	37.210656239833256	38.0	38.0	38.0	34.0	41.0
62-63	37.14723728680662	38.0	37.5	38.0	34.0	41.0
64-65	37.07113736099011	38.0	37.0	38.0	34.0	41.0
66-67	36.95946156177284	38.0	37.0	38.0	34.0	41.0
68-69	36.82710119940643	38.0	37.0	38.0	34.0	40.0
70-71	36.69250000012006	38.0	36.5	38.0	33.0	40.0
72-73	36.56248684622172	38.0	36.0	38.0	33.0	39.0
74-75	36.46169537219011	38.0	36.0	38.0	33.0	39.0
76-77	36.3602678892812	38.0	35.5	38.0	33.0	39.0
78-79	36.2552900828711	38.0	35.0	38.0	33.0	38.0
80-81	36.15378546114246	38.0	35.0	38.0	33.0	38.0
82-83	36.01140034464834	38.0	35.0	38.0	33.0	38.0
84-85	35.92055658091577	38.0	35.0	38.0	33.0	38.0
86-87	35.85957361246459	38.0	35.0	38.0	33.0	38.0
88-89	35.81393002730742	38.0	35.0	38.0	33.0	38.0
90-91	35.762926212072756	38.0	35.0	38.0	33.0	38.0
92-93	35.739196089994124	37.0	35.0	38.0	33.0	38.0
94-95	35.71205202108676	37.0	35.0	38.0	33.0	38.0
96-97	35.701462706862614	37.0	35.0	38.0	33.0	38.0
98-99	35.77722366656298	37.0	35.0	38.0	33.0	38.0
100	33.82015364717455	35.0	32.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	45.0
19	70.0
20	158.0
21	361.0
22	1236.0
23	3472.0
24	8208.0
25	16865.0
26	29061.0
27	46716.0
28	67764.0
29	93525.0
30	121814.0
31	153135.0
32	192369.0
33	245042.0
34	334583.0
35	517387.0
36	1088614.0
37	5004211.0
38	1128989.0
39	838899.0
40	89684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.040150716078685	20.839336928594893	14.267768079082263	25.852744276244156
2	13.775243843781688	20.43368662383473	43.05784715773482	22.73322237464876
3	17.008665218890492	26.791252354439486	31.731282644337334	24.468799782332685
4	11.94959272576601	19.42843558367024	37.704673986262435	30.917297704301316
5	12.631307288208202	37.86380629537623	36.293749226071945	13.211137190343623
6	28.402746672178097	38.268070932755904	18.81330786133275	14.515874533733253
7	24.919905035085907	32.94061146561389	22.616247519462867	19.523235979837334
8	23.581093691348805	36.91066299627147	21.946997123784076	17.561246188595657
9	24.41223330506967	16.72523429723652	20.81572864957917	38.04680374811464
10-11	23.57452390797303	26.708623089189164	29.223874638637394	20.492978364200415
12-13	24.632921968181396	23.78048548367546	28.926735662744026	22.659856885399122
14-15	21.899238270072892	25.38269947669417	25.732641936845162	26.985420316387774
16-17	21.87449566503593	29.19249304136711	26.368402572377548	22.564608721219408
18-19	22.092350078660267	27.655740716846278	28.08945912350904	22.16245008098441
20-21	22.44827300343298	26.687626369954877	27.976946163049394	22.88715446356275
22-23	22.185278301987694	27.881617011543103	27.73116955911813	22.201935127351074
24-25	22.433980450892292	28.29897831296229	27.86242873858923	21.404612497556187
26-27	22.44794808177151	28.69464937829886	27.77375636592448	21.083646174005153
28-29	22.172561609212476	28.105153302974312	27.86882771405216	21.85345737376106
30-31	23.18413154995263	27.542924854552066	27.979944047322665	21.292999548172638
32-33	22.271450608590225	27.79729712245272	28.327901429381903	21.603350839575153
34-35	22.170886598729787	26.770005893094268	28.785327938192477	22.27377956998346
36-37	21.56095843358729	28.03017396214944	28.579525713579056	21.829341890684216
38-39	21.70475576528798	26.53588122182618	29.255674984563584	22.50368802832226
40-41	21.885209309928232	26.463534927259047	29.391886293190666	22.259369469622055
42-43	22.559176843481392	26.230492825490785	29.310729910295823	21.899600420732
44-45	21.686235292936342	27.063418498538606	29.103684955681125	22.14666125284393
46-47	22.712999031111387	26.043372091518645	28.242577597573476	23.001051279796496
48-49	22.34253477299611	26.195446459063533	28.269773346688098	23.19224542125226
50-51	21.58789544836867	26.371552378142727	29.144817396622102	22.8957347768665
52-53	21.822288957459683	26.549400998007844	29.849028387311332	21.77928165722114
54-55	21.74199513092839	26.757681764400548	28.719532256288634	22.780790848382424
56-57	22.67693209410172	27.512256504361112	27.540797069042565	22.270014332494608
58-59	21.290554736161365	28.733482801497257	28.839024698014548	21.136937764326827
60-61	22.255909725517643	29.803724521223884	26.83077836323042	21.109587390028057
62-63	21.224016570191516	30.76840718892258	26.72931809578898	21.27825814509692
64-65	21.25736512254745	30.481956709005487	26.89398029255165	21.36669787589541
66-67	21.44977303728703	30.068680312273816	26.901988134565762	21.57955851587339
68-69	21.562385443743036	29.37705161529892	27.250269587820274	21.810293353137762
70-71	21.846379616165546	28.492338990038927	27.56329054704958	22.097990846745947
72-73	22.183532973566606	27.669103656474103	27.750307792863904	22.397055577095387
74-75	22.122797160622273	27.715056808308	27.77539195969722	22.386754071372508
76-77	22.305941288749263	27.662993805524533	27.58991217003446	22.441152735691748
78-79	22.23833826785255	27.66382155821674	27.591439813177587	22.50640036075312
80-81	22.23161323485382	27.60946952407991	27.66267976211453	22.496237478951738
82-83	22.218598944781753	27.611069650921017	27.766940956894803	22.40339044740243
84-85	22.445658326262258	27.618854310749125	27.605797985378633	22.329689377609988
86-87	22.217960701016825	27.721161325195443	27.658377681596146	22.40250029219158
88-89	22.32303502932516	27.628820195709963	27.649195113099363	22.398949661865515
90-91	22.328013734881434	27.719516572311242	27.64705395829678	22.305415734510543
92-93	22.433178045841533	27.638303571243185	27.631091734972628	22.297426647942654
94-95	22.284750553167466	27.69313818357323	27.661102636042855	22.36100862721645
96-97	22.294222865124276	27.758119419946205	27.788861035310084	22.15879667961944
98-99	23.398763950104502	29.660844055965164	27.94875641113291	18.991635582797425
100	22.39317315033013	30.565785268220143	21.673210775505876	25.367830805943854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1770.0
1	1637.0
2	2399.5
3	3490.0
4	4402.5
5	6392.0
6	8194.5
7	10362.0
8	12751.0
9	13679.0
10	12838.5
11	11677.5
12	12328.5
13	15133.0
14	21685.5
15	30578.5
16	37822.5
17	41713.5
18	42121.5
19	39875.5
20	36658.5
21	34229.0
22	34928.5
23	40487.5
24	51471.0
25	66629.0
26	85444.0
27	107197.5
28	127765.0
29	149692.0
30	170334.5
31	190001.5
32	213253.0
33	235127.0
34	257428.0
35	278513.0
36	298627.0
37	317920.5
38	331551.0
39	339478.5
40	349864.0
41	361107.5
42	378236.0
43	447638.0
44	505219.5
45	518992.5
46	501870.5
47	457331.0
48	424057.5
49	402052.5
50	392596.5
51	382060.0
52	369170.0
53	350933.5
54	331296.5
55	309120.0
56	283747.0
57	259118.0
58	233525.5
59	206080.5
60	174404.0
61	141912.5
62	112655.5
63	87450.0
64	66953.5
65	51374.0
66	39358.0
67	29981.0
68	22928.0
69	17145.5
70	12835.0
71	9784.0
72	7379.5
73	5298.0
74	3740.5
75	2640.5
76	1858.5
77	1261.5
78	856.5
79	618.0
80	471.0
81	345.0
82	250.5
83	185.5
84	137.0
85	99.0
86	79.5
87	59.0
88	32.0
89	19.5
90	16.5
91	14.0
92	10.0
93	6.0
94	3.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007212829459760075
3	7.012473085877851E-5
4	0.002414294305280803
5	0.006661849431583958
6	0.005349515182655389
7	0.0
8	0.0
9	2.103741925763355E-4
10-11	3.0554347017039207E-4
12-13	3.0053456082333648E-5
14-15	5.0089093470556074E-6
16-17	8.014254955288972E-5
18-19	4.508018412350047E-4
20-21	0.0
22-23	5.510498325885561E-4
24-25	9.319223694637299E-4
26-27	0.0
28-29	0.0013088352800210458
30-31	0.0019532941753922593
32-33	3.624780266575403E-4
34-35	3.0311663003417993E-5
36-37	7.208689008526051E-4
38-39	0.0013440565538113995
40-41	0.0010241693150535751
42-43	1.036898553474673E-5
44-45	0.0
46-47	9.084073469662389E-4
48-49	3.572395555683996E-4
50-51	0.003915436679025571
52-53	0.0016476375370853944
54-55	0.0012834452870824071
56-57	0.0011726599130998444
58-59	0.0012885020519395175
60-61	5.945038122556961E-4
62-63	8.222648917842693E-4
64-65	0.0026633614962764886
66-67	0.0021816131080648637
68-69	0.004432035273772642
70-71	0.002174079186625501
72-73	0.006745943977995979
74-75	0.008038206357383655
76-77	0.0033220692449334494
78-79	0.002821895431408756
80-81	2.776403207094369E-4
82-83	7.18270342330257E-5
84-85	4.620537682729064E-5
86-87	0.0013149282536431188
88-89	0.002964641550862748
90-91	4.1696076071141434E-4
92-93	7.059521176210356E-4
94-95	0.0014312654751194494
96-97	0.001623600136611289
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	212.0
20-21	752.0
22-23	1392.0
24-25	2531.0
26-27	4742.0
28-29	9222.0
30-31	24512.0
32-33	31916.0
34-35	43088.0
36-37	63167.0
38-39	68421.0
40-41	69325.0
42-43	79778.0
44-45	92391.0
46-47	91718.0
48-49	83366.0
50-51	73477.0
52-53	68856.0
54-55	73271.0
56-57	76877.0
58-59	84168.0
60-61	97667.0
62-63	101757.0
64-65	128048.0
66-67	150874.0
68-69	163240.0
70-71	185624.0
72-73	92419.0
74-75	81777.0
76-77	84181.0
78-79	87288.0
80-81	87858.0
82-83	83132.0
84-85	82898.0
86-87	86360.0
88-89	88521.0
90-91	90128.0
92-93	94360.0
94-95	113856.0
96-97	314081.0
98-99	686452.0
100-101	6038510.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.79651553764499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.58328728238305	70.76424431749086
2	7.965396859251241	12.871526222040028
3	2.2837219412261325	5.535503259238139
4	0.9226677548995827	2.981933583793126
5	0.465891002469082	1.8821184809921094
6	0.2577551993918898	1.2495433183545361
7	0.1526559660214495	0.8633849091396375
8	0.09835755130879599	0.635755793805268
9	0.06241578849961786	0.45386804027733646
>10	0.20411657966279148	2.45198816283957
>50	0.002974403939652228	0.16235518814361388
>100	6.886330102281289E-4	0.08975155259214168
>500	4.735893035190938E-5	0.026344890985588253
>1k	2.367900602267335E-5	0.03168228030812574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0053456082333648E-5	0.0	0.0	0.0
3	0.0	3.0053456082333648E-5	0.0	1.0017818694111215E-5	0.0
4	0.0	9.016036824700094E-5	0.0	2.003563738822243E-5	0.0
5	1.0017818694111215E-5	1.0017818694111216E-4	0.0	2.003563738822243E-5	0.0
6	1.0017818694111215E-5	1.0017818694111216E-4	0.0	2.003563738822243E-5	0.0
7	1.0017818694111215E-5	1.0017818694111216E-4	0.0	3.0053456082333648E-5	0.0
8	1.0017818694111215E-5	1.2021382432933459E-4	0.0	4.007127477644486E-5	0.0
9	1.0017818694111215E-5	2.4042764865866918E-4	0.0	9.016036824700094E-5	0.0
10-11	3.0053456082333648E-5	2.4042764865866918E-4	0.0	9.016036824700094E-5	0.0
12-13	4.007127477644486E-5	2.4042764865866918E-4	0.0	9.516927759405656E-5	0.0
14-15	4.508018412350047E-5	3.5062365429389255E-4	0.0	1.1019600563522337E-4	0.0
16-17	5.5098002817611684E-5	3.606414729880038E-4	0.0	1.4024946171755702E-4	0.0
18-19	6.0106912164667296E-5	3.7065929168211495E-4	0.0	2.0536528322927992E-4	0.0
20-21	6.0106912164667296E-5	3.95703838417393E-4	0.0	2.3040982996455796E-4	0.0
22-23	6.511582151172291E-5	4.958820253585052E-4	0.0	2.5044546735278036E-4	0.0
24-25	8.014254955288972E-5	5.609978468702281E-4	0.0	3.2557910755861446E-4	0.0
26-27	9.016036824700094E-5	5.910513029525617E-4	0.0	4.4078402254089347E-4	0.0
28-29	9.016036824700094E-5	5.910513029525617E-4	0.0	0.0011219956937404561	0.0
30-31	9.016036824700094E-5	6.411403964231178E-4	0.0	0.003386022718609591	0.0
32-33	1.0017818694111216E-4	6.51158215117229E-4	0.0	0.008344842972194642	0.0
34-35	1.1019600563522337E-4	7.763809487936192E-4	0.0	0.01532726260199016	0.0
36-37	1.2021382432933459E-4	9.016036824700094E-4	0.0	0.02573076731582466	0.0
38-39	1.2021382432933459E-4	9.516927759405655E-4	0.0	0.051716989008349146	0.0
40-41	1.2021382432933459E-4	0.0010318353254934551	0.0	0.0772674355876798	0.0
42-43	1.2021382432933459E-4	0.0010618887815757889	0.0	0.09402724626292787	0.0
44-45	1.3023164302344582E-4	0.001227182790028624	0.0	0.11511976352337903	0.0
46-47	1.4024946171755702E-4	0.0013724411610932365	0.0	0.13632748569881248	0.0
48-49	1.4525837106461263E-4	0.0013824589797873476	0.0	0.15367333876766603	0.0
50-51	1.5026728041166824E-4	0.0015677886256284053	0.0	0.1699372674175556	0.0
52-53	1.6028509910577944E-4	0.0015978420817107388	0.0	0.21961563032165313	0.0
54-55	1.6028509910577944E-4	0.0016128688097519057	0.0	0.2762864306742403	0.0
56-57	1.6028509910577944E-4	0.0016329044471401282	0.0	0.3575559848302175	0.0
58-59	1.803207364940019E-4	0.0016429222658342394	0.0	0.40116855851503064	0.0
60-61	1.803207364940019E-4	0.0016880024499577398	0.0	0.4175176386238202	0.0
62-63	1.803207364940019E-4	0.0017080380873459623	0.0	0.4282667580826015	0.0
64-65	1.803207364940019E-4	0.0018833499144929086	0.0	0.4339719058288979	0.0
66-67	1.803207364940019E-4	0.0018983766425340752	0.0	0.43888063698901236	0.0
68-69	1.9033855518811309E-4	0.0019434568266575758	0.0	0.44380439487716805	0.0
70-71	1.9033855518811309E-4	0.001953474645351687	0.0	0.44831241328951804	0.0
72-73	2.003563738822243E-4	0.001963492464045798	0.0	0.45203904184372745	0.0
74-75	2.003563738822243E-4	0.0020336171949045766	0.0	0.45491415580893735	0.0
76-77	2.003563738822243E-4	0.0021438132005398	0.0	0.45764401140308264	0.0
78-79	2.2039201127044673E-4	0.0021638488379280224	0.0	0.4594522276773697	0.0
80-81	2.2039201127044673E-4	0.0021638488379280224	0.0	0.46011340371118103	0.0
82-83	2.3040982996455796E-4	0.0021688577472750784	0.0	0.4606844193767454	0.0
84-85	2.4042764865866918E-4	0.002173866656622134	0.0	0.46090982029736294	0.0
86-87	2.4042764865866918E-4	0.0022139379313985785	0.0	0.46098996284691585	0.0
88	2.4042764865866918E-4	0.0023141161183396907	0.0	0.46099998066560993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	26520	0.0	56.563885	1
GGTATCA	12400	0.0	50.79076	1
TTATACA	31655	0.0	47.692085	2
TATACAC	32475	0.0	47.438644	3
GTATCAA	22860	0.0	37.173107	1
TACACAT	45440	0.0	33.985706	5
ATACACA	47530	0.0	32.949436	4
ACACATC	50565	0.0	30.108944	6
ATCAACG	27545	0.0	30.074854	3
TCAACGC	27675	0.0	29.994173	4
CAACGCA	28045	0.0	29.568043	5
ACATCTC	51400	0.0	29.552666	8
CACATCT	52405	0.0	28.92209	7
TATCAAC	29000	0.0	28.841206	2
AACGCAG	29065	0.0	28.585934	6
CATCTCC	55835	0.0	27.055515	9
CGCAGAG	33935	0.0	24.407932	8
ACGCAGA	34080	0.0	24.291815	7
GTGGTAT	5650	0.0	21.53902	1
GCAGAGT	39110	0.0	21.167606	9
>>END_MODULE