FastQCFastQC Report
Fri 27 May 2016
SRR2138954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4602548
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58700.050.1136631
GTATCAA102800.036.161071
TCAACGC126600.028.7575444
ATCAACG127800.028.5206833
CAACGCA129350.028.1789195
TATCAAC134100.027.467922
AACGCAG133700.027.387126
ACGCAGA152050.023.96817
CGCAGAG155050.023.4496888
GTGGTAT26850.023.2064021
GTACATG126050.020.1763761
GCAGAGT181150.020.0242829
TGGTATC27400.019.956022
AGACCGA43200.019.44582788-89
TACATGG125700.019.3895322
GAGTACT113800.019.38248412-13
ACCGAGG44550.019.37609990-91
ACATGGG123500.019.1495913
CGAGACC45450.019.04869386-87
GACCGAG42900.018.89773890-91
Produced by FastQC (version 0.11.5)