##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4602548
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43818619599404	34.0	31.0	34.0	31.0	34.0
2	32.56921318365392	34.0	31.0	34.0	31.0	34.0
3	32.61419174770149	34.0	31.0	34.0	31.0	34.0
4	36.14289671720969	37.0	35.0	37.0	35.0	37.0
5	36.05393338646332	37.0	35.0	37.0	35.0	37.0
6	36.06809814911219	37.0	35.0	37.0	35.0	37.0
7	36.029465852393066	37.0	35.0	37.0	35.0	37.0
8	36.026664360697595	37.0	35.0	37.0	35.0	37.0
9	37.75314065165643	39.0	38.0	39.0	35.0	39.0
10-11	37.68295333367517	39.0	38.0	39.0	35.0	39.0
12-13	37.66429041044222	39.0	37.0	39.0	35.0	39.0
14-15	39.08286594729702	41.0	38.0	41.0	36.0	41.0
16-17	39.05713128901643	41.0	38.0	41.0	35.0	41.0
18-19	39.06672401895646	41.0	38.5	41.0	35.0	41.0
20-21	39.05096017492558	41.0	39.0	41.0	35.0	41.0
22-23	39.03456660609153	41.0	39.0	41.0	35.0	41.0
24-25	38.99242995265058	41.0	39.0	41.0	35.0	41.0
26-27	38.93236374739544	41.0	38.0	41.0	35.0	41.0
28-29	38.874198263482455	40.5	38.0	41.0	35.0	41.0
30-31	38.79398122440226	40.0	38.0	41.0	34.5	41.0
32-33	38.69962863835539	40.0	38.0	41.0	34.0	41.0
34-35	38.62428723906818	40.0	38.0	41.0	34.0	41.0
36-37	38.601208082473875	40.0	38.0	41.0	34.0	41.0
38-39	38.552407049190656	40.0	38.0	41.0	34.0	41.0
40-41	38.534955759069604	40.0	38.0	41.0	34.0	41.0
42-43	38.481666819211995	40.0	38.0	41.0	34.0	41.0
44-45	38.39090918519493	40.0	38.0	41.0	33.0	41.0
46-47	38.34224379889354	40.0	38.0	41.0	33.0	41.0
48-49	38.28821021685422	40.0	38.0	41.0	33.0	41.0
50-51	38.22843743661299	40.0	38.0	41.0	33.0	41.0
52-53	38.10288049723886	40.0	37.0	41.0	33.0	41.0
54-55	37.97872332530372	40.0	37.0	41.0	33.0	41.0
56-57	37.85329135288013	40.0	37.0	41.0	33.0	41.0
58-59	37.739480853732715	40.0	36.0	41.0	32.0	41.0
60-61	37.6090094869744	39.5	36.0	41.0	32.0	41.0
62-63	37.40179343162889	39.0	35.5	41.0	32.0	41.0
64-65	37.19543918433596	39.0	35.0	41.0	32.0	41.0
66-67	36.97398680870455	38.5	35.0	41.0	32.0	41.0
68-69	36.65276773964655	37.5	35.0	40.0	31.0	41.0
70-71	36.33067521596749	37.0	35.0	40.0	31.0	41.0
72-73	36.031182319383475	37.0	35.0	39.0	31.0	41.0
74-75	35.766642681783686	36.0	35.0	39.0	31.0	41.0
76-77	35.47564328947516	36.0	34.5	39.0	31.0	40.5
78-79	35.207372419459254	35.0	34.0	37.5	31.0	39.5
80-81	34.92393911477928	35.0	34.0	37.0	31.0	39.0
82-83	34.6180465828092	35.0	34.0	37.0	31.0	39.0
84-85	34.386752559137975	35.0	34.0	36.0	30.0	37.5
86-87	34.22091974119969	35.0	34.0	36.0	31.0	37.0
88-89	34.074745161230226	35.0	34.0	36.0	31.0	37.0
90-91	33.93562019699782	35.0	34.0	35.0	30.0	36.0
92-93	33.88388547716508	35.0	34.0	35.0	31.0	36.0
94-95	33.82921890675429	35.0	34.0	35.0	31.0	36.0
96-97	33.821711159225984	35.0	34.0	35.0	31.0	36.0
98-99	33.97018081274079	35.0	34.0	35.0	31.0	36.0
100	31.195416333307588	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	32.0
20	54.0
21	147.0
22	584.0
23	1993.0
24	5233.0
25	10924.0
26	20118.0
27	32151.0
28	46493.0
29	62904.0
30	81008.0
31	101295.0
32	125658.0
33	158415.0
34	206119.0
35	285128.0
36	416092.0
37	772127.0
38	1255484.0
39	935521.0
40	85060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.20394790016313	20.795329022098194	14.85768317896956	24.143039898769118
2	14.729671914182676	21.428343278850456	41.07203425461639	22.769950552350473
3	17.827073274121087	26.28590436713301	30.837587688013073	25.04943467073283
4	12.67721705455326	18.51667815305783	38.77232784970412	30.033776942684792
5	13.017847783629305	37.863307220208135	35.87724803065083	13.241596965511725
6	29.489622125327198	38.33707590739856	18.866375981583666	13.306925985690576
7	25.90699760219774	32.97697275509131	23.58030812497773	17.535721517733222
8	24.038793294496873	37.012606929900564	21.358212885558174	17.590386890044385
9	25.29581440541196	16.939921104570775	21.674385579465984	36.089878910551285
10-11	23.894956846172004	26.99005865557252	29.150873326320546	19.964111171934928
12-13	24.651774332777045	24.60396384871246	29.339885067985183	21.404376750525305
14-15	21.88070607845915	25.958794997901162	26.99404764491321	25.16645127872648
16-17	21.345081778792483	28.97274286491497	27.760020666858154	21.922154689434397
18-19	21.395064058044035	28.18273655512199	29.425787758428534	20.996411628405443
20-21	22.191634706037902	27.241732733601214	29.46676177900635	21.099870781354536
22-23	21.992570865911585	27.157846607265757	29.220797142219478	21.62878538460318
24-25	21.74844822095638	27.26859716565968	29.308891794455437	21.674062818928498
26-27	21.6931229588538	27.505957205549198	29.19529826043291	21.60562157516409
28-29	21.65266237812874	27.56082939976755	29.215076121965332	21.57143210013838
30-31	21.910918553938348	27.305603008389834	29.124653746856183	21.658824690815635
32-33	21.749373758290307	27.45304265915595	29.07490791398452	21.722675668569217
34-35	21.855516208475954	27.458606105044648	29.081930703107105	21.603946983372296
36-37	21.871512725705614	27.470321110832053	28.956910874091307	21.701255289371026
38-39	21.781966980672262	27.541263082335632	29.008345666102457	21.668424270889645
40-41	21.988598337720333	27.43805143660365	28.867652538991805	21.70569768668421
42-43	21.86408553381251	27.523800260874626	28.710752845889676	21.90136135942319
44-45	22.011420048781858	27.58672103792422	28.497653358760566	21.904205554533352
46-47	22.040232824969976	27.6688275141968	28.22882599443961	22.062113666393614
48-49	22.03140399424607	27.768294444219784	28.150422590540675	22.04987897099348
50-51	21.98258210452835	27.892068715963376	28.032183366877096	22.09316581263118
52-53	22.022899410353798	27.808320028321422	27.993968081587482	22.174812479737295
54-55	22.097266288441062	27.770969559282637	27.985277695507698	22.146486456768603
56-57	22.061214417007	27.728450895240332	27.989506317784258	22.22082836996842
58-59	21.97202570434629	27.760996206517426	28.006207822210964	22.260770266925324
60-61	22.1842875633135	27.65821898889943	28.167891239968178	21.989602207818887
62-63	21.946344048685102	27.726932144227785	28.115340801793405	22.211383005293715
64-65	21.92843220425438	27.756089250916986	28.031031377744103	22.284447167084526
66-67	21.981655779811778	27.85005898235642	27.99636699460013	22.171918243231673
68-69	21.94053913878124	27.987598000649506	27.86660869340698	22.205254167162273
70-71	21.909909129939933	27.781439987686873	27.803957515664806	22.504693366708388
72-73	22.04271971836333	27.692415062326383	27.712687544425947	22.552177674884348
74-75	21.943941961109857	27.854514038386284	27.887786350969467	22.313757649534395
76-77	22.123392214095553	27.622728667882406	27.733402282682423	22.52047683533962
78-79	22.027370548806612	27.597118953412505	27.57895854960314	22.796551948177747
80-81	22.208482672642237	27.858742504715888	27.579433853264007	22.353340969377864
82-83	22.208644596436592	27.668961372268537	27.696586779827527	22.425807251467347
84-85	22.176061796894704	27.694515664575913	27.5782213690189	22.551201169510485
86-87	22.036655992519275	27.93390188606009	27.63844688950052	22.39099523192011
88-89	22.271919474265275	27.895456417801483	27.60298466309375	22.229639444839492
90-91	22.364266942586838	27.900749613613257	27.555797554413335	22.17918588938657
92-93	22.200128528327706	27.6100470037443	27.749343378540015	22.44048108938798
94-95	22.217907348969074	27.922448273008005	27.76418390316861	22.095460474854313
96-97	22.439562331429677	27.818580848785103	27.90160259570886	21.840254224076368
98-99	23.129181637431376	29.830722052692515	28.254815085103257	18.785281224772852
100	22.196470799161812	30.903680071658325	21.658253852733356	25.241595276446503
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1059.0
1	864.5
2	1064.5
3	1482.0
4	1838.0
5	3023.0
6	4126.0
7	5051.0
8	5660.0
9	5533.5
10	4851.5
11	3897.5
12	3836.5
13	5076.0
14	8822.5
15	14471.0
16	19383.0
17	22498.0
18	23785.5
19	23420.0
20	21873.0
21	20062.0
22	19556.0
23	21415.0
24	25595.0
25	32188.5
26	40704.0
27	50202.0
28	60586.0
29	72289.5
30	83630.5
31	94384.5
32	105102.5
33	115719.0
34	126330.5
35	135407.0
36	144105.0
37	153137.5
38	160714.5
39	166142.0
40	172002.5
41	177694.5
42	180503.0
43	182151.0
44	184580.5
45	187906.0
46	188503.0
47	187517.0
48	186657.5
49	183807.0
50	180823.5
51	176423.0
52	169226.0
53	160981.0
54	152174.5
55	140724.5
56	128344.0
57	116700.5
58	104484.0
59	91593.0
60	76401.0
61	61712.0
62	49587.0
63	39214.0
64	30673.0
65	24003.0
66	18728.5
67	14488.5
68	11312.5
69	8665.5
70	6664.5
71	5145.5
72	3936.0
73	3009.5
74	2310.0
75	1773.0
76	1312.0
77	959.5
78	733.5
79	581.0
80	449.0
81	334.5
82	252.0
83	177.5
84	122.5
85	81.0
86	56.0
87	43.0
88	32.5
89	22.5
90	13.5
91	9.5
92	8.5
93	4.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006670218322546555
3	1.9554385961862865E-4
4	0.0
5	0.011298089666854099
6	0.011862994150196805
7	0.0
8	0.0
9	0.0
10-11	3.259064326977144E-5
12-13	2.172709551318096E-5
14-15	0.0
16-17	2.172709551318096E-4
18-19	9.234015593101908E-4
20-21	0.0
22-23	1.955832095726246E-4
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.576109258109658E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.006350963569358057
52-53	4.695193882162372E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	2.4154504772869756E-5
62-63	9.137510393918074E-4
64-65	0.0012413970264239346
66-67	9.927501935242408E-4
68-69	0.00993835961170967
70-71	6.961525672777662E-4
72-73	3.7068083332119034E-4
74-75	2.452181492921649E-4
76-77	5.215530493772526E-5
78-79	0.0
80-81	5.330569255488721E-5
82-83	0.0
84-85	0.0
86-87	9.648625987416262E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	157.0
20-21	548.0
22-23	1083.0
24-25	1919.0
26-27	2911.0
28-29	4654.0
30-31	10965.0
32-33	14040.0
34-35	19111.0
36-37	26603.0
38-39	28693.0
40-41	31465.0
42-43	36690.0
44-45	43507.0
46-47	42894.0
48-49	38042.0
50-51	32126.0
52-53	30349.0
54-55	31321.0
56-57	34605.0
58-59	21725.0
60-61	36398.0
62-63	35472.0
64-65	38100.0
66-67	39643.0
68-69	39365.0
70-71	39064.0
72-73	37429.0
74-75	38958.0
76-77	40393.0
78-79	41792.0
80-81	41914.0
82-83	40924.0
84-85	41492.0
86-87	43732.0
88-89	46097.0
90-91	48490.0
92-93	52792.0
94-95	67854.0
96-97	182459.0
98-99	374490.0
100-101	2822282.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.37068362828232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.67721147496974	79.22529956074432
2	6.817539004435985	11.913060869601027
3	1.538317387280806	4.0321152529199145
4	0.4854206972741498	1.696461526726398
5	0.20069125681462374	0.8767266153056427
6	0.10647633243221669	0.5581745972901012
7	0.05887482129386647	0.3600753369456646
8	0.03555405177481364	0.248510544745665
9	0.021960165700359437	0.1726807220847685
>10	0.0563374464876969	0.7185795906610944
>50	9.037259273325032E-4	0.05069161864046132
>100	7.136356083835014E-4	0.14762376433499597
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.345419102636192E-5
2	2.172709551318096E-5	0.0	0.0	0.0	4.345419102636192E-5
3	2.172709551318096E-5	0.0	0.0	0.0	4.345419102636192E-5
4	2.172709551318096E-5	4.345419102636192E-5	0.0	0.0	4.345419102636192E-5
5	2.172709551318096E-5	4.345419102636192E-5	0.0	2.172709551318096E-5	4.345419102636192E-5
6	2.172709551318096E-5	4.345419102636192E-5	0.0	2.172709551318096E-5	4.345419102636192E-5
7	2.172709551318096E-5	4.345419102636192E-5	0.0	6.518128653954288E-5	4.345419102636192E-5
8	2.172709551318096E-5	4.345419102636192E-5	0.0	6.518128653954288E-5	6.518128653954288E-5
9	2.172709551318096E-5	4.345419102636192E-5	0.0	6.518128653954288E-5	6.518128653954288E-5
10-11	2.172709551318096E-5	4.345419102636192E-5	0.0	6.518128653954288E-5	6.518128653954288E-5
12-13	2.172709551318096E-5	4.345419102636192E-5	0.0	6.518128653954288E-5	6.518128653954288E-5
14-15	2.172709551318096E-5	6.518128653954288E-5	0.0	6.518128653954288E-5	6.518128653954288E-5
16-17	2.172709551318096E-5	6.518128653954288E-5	0.0	6.518128653954288E-5	6.518128653954288E-5
18-19	2.172709551318096E-5	6.518128653954288E-5	0.0	7.604483429613336E-5	6.518128653954288E-5
20-21	2.172709551318096E-5	6.518128653954288E-5	0.0	8.690838205272384E-5	7.604483429613336E-5
22-23	3.259064326977144E-5	8.690838205272384E-5	0.0	1.5208966859226673E-4	1.086354775659048E-4
24-25	6.518128653954288E-5	8.690838205272384E-5	0.0	2.4986159840158104E-4	1.3036257307908576E-4
26-27	6.518128653954288E-5	8.690838205272384E-5	0.0	4.019512669938477E-4	1.3036257307908576E-4
28-29	6.518128653954288E-5	8.690838205272384E-5	0.0	9.885828458497336E-4	1.3036257307908576E-4
30-31	6.518128653954288E-5	8.690838205272384E-5	0.0	0.0037261968805105345	1.3036257307908576E-4
32-33	6.518128653954288E-5	1.086354775659048E-4	0.0	0.010081372318115966	1.3036257307908576E-4
34-35	6.518128653954288E-5	1.086354775659048E-4	0.0	0.01876134697563176	1.3036257307908576E-4
36-37	6.518128653954288E-5	1.086354775659048E-4	0.0	0.03370958868870026	1.3036257307908576E-4
38-39	6.518128653954288E-5	1.086354775659048E-4	0.0	0.07287267835120895	1.3036257307908576E-4
40-41	6.518128653954288E-5	1.3036257307908576E-4	0.0	0.11384998048906822	1.3036257307908576E-4
42-43	6.518128653954288E-5	1.3036257307908576E-4	0.0	0.1382060545593441	1.3036257307908576E-4
44-45	6.518128653954288E-5	1.5208966859226673E-4	0.0	0.17111174071405666	1.4122612083567626E-4
46-47	6.518128653954288E-5	1.7381676410544767E-4	0.0	0.20302884402291949	1.7381676410544767E-4
48-49	6.518128653954288E-5	1.7381676410544767E-4	0.0	0.22838436448680166	1.9554385961862865E-4
50-51	6.518128653954288E-5	1.9554385961862865E-4	0.0	0.2527187114615643	1.9554385961862865E-4
52-53	6.518128653954288E-5	1.9554385961862865E-4	0.0	0.334564680259717	2.0640740737521912E-4
54-55	6.518128653954288E-5	1.9554385961862865E-4	0.0	0.43093521240843113	2.172709551318096E-4
56-57	9.777192980931431E-5	1.9554385961862865E-4	0.0	0.5478813040081276	2.172709551318096E-4
58-59	1.3036257307908576E-4	1.9554385961862865E-4	0.0	0.7647503078729434	2.172709551318096E-4
60-61	1.3036257307908576E-4	1.9554385961862865E-4	0.0	0.9386865710037136	2.172709551318096E-4
62-63	1.3036257307908576E-4	2.172709551318096E-4	0.0	0.9450634735368322	2.3899805064499057E-4
64-65	1.3036257307908576E-4	2.607251461581715E-4	0.0	0.9510166977074438	2.3899805064499057E-4
66-67	1.3036257307908576E-4	2.607251461581715E-4	0.0	0.9559596119366924	2.3899805064499057E-4
68-69	1.3036257307908576E-4	2.71588693914762E-4	0.0	0.9614782941970403	2.3899805064499057E-4
70-71	1.3036257307908576E-4	2.8245224167135246E-4	0.0	0.9662691187576968	2.607251461581715E-4
72-73	1.3036257307908576E-4	2.8245224167135246E-4	0.0	0.9695173195369173	3.0417933718453346E-4
74-75	1.3036257307908576E-4	2.8245224167135246E-4	0.0	0.9726460212908155	3.0417933718453346E-4
76-77	1.3036257307908576E-4	2.8245224167135246E-4	0.0	0.975948539808819	3.1504288494112393E-4
78-79	1.3036257307908576E-4	2.8245224167135246E-4	0.0	0.9777627522841695	3.259064326977144E-4
80-81	1.5208966859226673E-4	2.8245224167135246E-4	0.0	0.9782842025764859	3.259064326977144E-4
82-83	1.7381676410544767E-4	2.8245224167135246E-4	0.0	0.9792076041357961	3.693606237240763E-4
84-85	1.7381676410544767E-4	2.8245224167135246E-4	2.172709551318096E-5	0.9794357386386845	3.693606237240763E-4
86-87	1.9554385961862865E-4	2.8245224167135246E-4	2.172709551318096E-5	0.9795443741162504	3.693606237240763E-4
88	1.9554385961862865E-4	2.8245224167135246E-4	2.172709551318096E-5	0.9795443741162504	3.693606237240763E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5870	0.0	50.113663	1
GTATCAA	10280	0.0	36.16107	1
TCAACGC	12660	0.0	28.757544	4
ATCAACG	12780	0.0	28.520683	3
CAACGCA	12935	0.0	28.178919	5
TATCAAC	13410	0.0	27.46792	2
AACGCAG	13370	0.0	27.38712	6
ACGCAGA	15205	0.0	23.9681	7
CGCAGAG	15505	0.0	23.449688	8
GTGGTAT	2685	0.0	23.206402	1
GTACATG	12605	0.0	20.176376	1
GCAGAGT	18115	0.0	20.024282	9
TGGTATC	2740	0.0	19.95602	2
AGACCGA	4320	0.0	19.445827	88-89
TACATGG	12570	0.0	19.389532	2
GAGTACT	11380	0.0	19.382484	12-13
ACCGAGG	4455	0.0	19.376099	90-91
ACATGGG	12350	0.0	19.149591	3
CGAGACC	4545	0.0	19.048693	86-87
GACCGAG	4290	0.0	18.897738	90-91
>>END_MODULE