FastQCFastQC Report
Fri 27 May 2016
SRR2138953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9302547
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112900.051.05481
GTATCAA207150.036.1995961
TCAACGC255400.028.5902044
ATCAACG255450.028.5846083
CAACGCA261350.027.9393065
TATCAAC269250.027.602112
AACGCAG269800.027.1534166
CTTATAC116150.025.946711
ACGCAGA309250.023.646597
CGCAGAG313650.023.3015428
TATACAC147900.022.8060763
GTGGTAT55700.021.1621671
GCAGAGT370650.019.7181429
TTATACA159800.019.6701342
TGGTATC55100.019.344382
GAGTACT223300.019.06900212-13
GTACATG251100.018.4608231
AGAGTAC337600.018.1641810-11
CAGAGTA360350.018.01469610-11
TACACAT185600.017.9033555
Produced by FastQC (version 0.11.5)