##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138953_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9302547
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4469944629143	34.0	31.0	34.0	31.0	34.0
2	32.577958703137966	34.0	31.0	34.0	31.0	34.0
3	32.622840067349294	34.0	31.0	34.0	31.0	34.0
4	36.14897156660429	37.0	35.0	37.0	35.0	37.0
5	36.059449094962915	37.0	35.0	37.0	35.0	37.0
6	36.07410776854984	37.0	35.0	37.0	35.0	37.0
7	36.03050293645386	37.0	35.0	37.0	35.0	37.0
8	36.02694778107544	37.0	35.0	37.0	35.0	37.0
9	37.7568297155607	39.0	38.0	39.0	35.0	39.0
10-11	37.686188470748924	39.0	38.0	39.0	35.0	39.0
12-13	37.66490042995751	39.0	37.0	39.0	35.0	39.0
14-15	39.08733264126481	41.0	38.0	41.0	36.0	41.0
16-17	39.06099286571731	41.0	38.0	41.0	35.0	41.0
18-19	39.06659133245981	41.0	38.5	41.0	35.0	41.0
20-21	39.05231470175329	41.0	39.0	41.0	35.0	41.0
22-23	39.03809953467677	41.0	39.0	41.0	35.0	41.0
24-25	38.99327943635319	41.0	39.0	41.0	35.0	41.0
26-27	38.92644201186097	41.0	38.0	41.0	35.0	41.0
28-29	38.864118531318596	40.5	38.0	41.0	35.0	41.0
30-31	38.78946893312833	40.0	38.0	41.0	34.5	41.0
32-33	38.69532499608623	40.0	38.0	41.0	34.0	41.0
34-35	38.6168072382095	40.0	38.0	41.0	34.0	41.0
36-37	38.593738917354074	40.0	38.0	41.0	34.0	41.0
38-39	38.5373353904557	40.0	38.0	41.0	34.0	41.0
40-41	38.520827318335634	40.0	38.0	41.0	34.0	41.0
42-43	38.47122448163127	40.0	38.0	41.0	34.0	41.0
44-45	38.38335142241887	40.0	38.0	41.0	33.0	41.0
46-47	38.336615450310674	40.0	38.0	41.0	33.0	41.0
48-49	38.28849876221932	40.0	38.0	41.0	33.0	41.0
50-51	38.21558918658206	40.0	38.0	41.0	33.0	41.0
52-53	38.095927247888454	40.0	37.0	41.0	33.0	41.0
54-55	37.97540350529258	40.0	37.0	41.0	33.0	41.0
56-57	37.84892375868523	40.0	37.0	41.0	33.0	41.0
58-59	37.73752070899225	40.0	36.0	41.0	32.0	41.0
60-61	37.59533205160543	39.5	36.0	41.0	32.0	41.0
62-63	37.39781671155349	39.0	35.5	41.0	32.0	41.0
64-65	37.19260773907011	39.0	35.0	41.0	32.0	41.0
66-67	36.964482497603925	38.5	35.0	41.0	32.0	41.0
68-69	36.642216533929734	37.5	35.0	40.0	31.0	41.0
70-71	36.32623193755964	37.0	35.0	40.0	31.0	41.0
72-73	36.02963373760849	37.0	35.0	39.0	31.0	41.0
74-75	35.76741401333482	36.0	35.0	39.0	31.0	41.0
76-77	35.47924764476219	36.0	35.0	39.0	31.0	40.5
78-79	35.20722022104965	35.0	34.0	37.5	31.0	39.5
80-81	34.93051542258726	35.0	34.0	37.0	31.0	39.0
82-83	34.63490560701936	35.0	34.0	36.5	31.0	39.0
84-85	34.401754133742116	35.0	34.0	36.0	31.0	37.5
86-87	34.23529207723881	35.0	34.0	36.0	31.0	37.0
88-89	34.096518091470145	35.0	34.0	36.0	31.0	37.0
90-91	33.96288970074286	35.0	34.0	35.0	31.0	36.0
92-93	33.90756838150197	35.0	34.0	35.0	31.0	36.0
94-95	33.85510521686621	35.0	34.0	35.0	31.0	36.0
96-97	33.853340873833616	35.0	34.0	35.0	31.0	36.0
98-99	33.98865930858586	35.0	34.0	35.0	31.0	36.0
100	31.21799328677301	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	17.0
19	45.0
20	107.0
21	322.0
22	1253.0
23	4276.0
24	10889.0
25	23023.0
26	40752.0
27	64807.0
28	93493.0
29	126244.0
30	161830.0
31	202514.0
32	251959.0
33	315890.0
34	411672.0
35	566470.0
36	829528.0
37	1541721.0
38	2532739.0
39	1924338.0
40	198655.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.072122183311734	20.787414457567372	14.799688730408993	24.340774628711902
2	14.600768786857952	21.326976514976252	41.295019699141946	22.777234999023857
3	17.71873697599073	26.18614224451389	31.1186278229011	24.97649295659429
4	12.5108317109282	18.713487822206112	38.49963886234598	30.276041604519712
5	12.958473059480118	37.8619297818595	35.88778101834451	13.29181614031587
6	29.375554221458078	38.43104904499741	18.731223127516305	13.46217360602821
7	25.77605896535648	32.97771029805063	23.340973176485967	17.90525756010693
8	23.92827469724152	37.09130413423335	21.415951996802598	17.564469171722543
9	24.978632196107153	16.93917805521434	21.506475592114718	36.57571415656379
10-11	23.77803420710351	26.96967812505862	29.192533029817714	20.05975463802016
12-13	24.581070265237646	24.542080932802847	29.226799183814705	21.6500496181448
14-15	21.873784774953826	25.944772219090762	26.749476608367395	25.431966397588013
16-17	21.472238151293034	29.09188028388211	27.507229624305708	21.92865194051915
18-19	21.548520679399804	28.056636931834916	29.211542856577722	21.183299532187554
20-21	22.201426149514567	27.159349041117096	29.177819322143588	21.46140548722475
22-23	21.852556966587446	27.28025076765526	29.07780890433336	21.78938336142393
24-25	21.827038537753985	27.390150058749036	29.145775581373464	21.637035822123515
26-27	21.6698062483396	27.501906936752107	29.17214035614014	21.65614645876815
28-29	21.443439087486013	27.316458942986827	29.24030247140641	21.999799498120755
30-31	21.771370035115087	27.14892205520445	29.24132875398136	21.8383791556991
32-33	21.62194319901751	27.36557146581029	29.167500525182206	21.84498480999
34-35	21.72451890068198	27.588533454452012	29.20415620208962	21.482791442776385
36-37	21.804601851258322	27.391871844817683	28.905143832925397	21.898382470998595
38-39	21.776258909045897	27.369446012095377	29.041659840389666	21.81263523846906
40-41	21.90766133865753	27.244453991850893	29.01857862783837	21.829306041653208
42-43	21.971022034920345	27.30723559128717	28.8644708066364	21.85727156715608
44-45	21.856680659273376	27.526896239557686	28.66180506312975	21.95461803803919
46-47	22.14975287437153	27.38520769491954	28.217402360870192	22.247637069838742
48-49	22.055111595580723	27.55013620252958	28.223532119950555	22.17122008193914
50-51	21.890238123187554	27.69096013019894	28.239905741999156	22.17889600461435
52-53	21.992875359599427	27.716381691963544	28.24134073352437	22.04940221491266
54-55	21.991243632605237	27.67789389604572	28.10733316433611	22.223529307012928
56-57	22.126392180341586	27.84520775728778	27.89934760795535	22.129052454415284
58-59	21.841667762119663	28.061700961708553	28.198030011865754	21.89860126430603
60-61	22.095239020958587	28.1606386568814	27.8469296288545	21.897192693305517
62-63	21.917531654672327	28.331837915948192	27.772635500805638	21.977994928573843
64-65	21.841105726295307	28.277312037430125	27.828990293993076	22.05259194228149
66-67	21.99202894592604	28.235123492949043	27.65942579027765	22.113421770847264
68-69	21.918629138052147	28.108514490081287	27.791781603728715	22.181074768137844
70-71	21.967681236543992	27.749907668589042	27.89227259195365	22.390138502913317
72-73	22.077898293211813	27.64670070505863	27.86722746002594	22.40817354170361
74-75	21.917761100214015	27.805696842165467	27.89482552200999	22.38171653561052
76-77	22.09453562883903	27.752003154813853	27.765521500168376	22.38793971617874
78-79	22.012733969793423	27.778557444688477	27.796228145411717	22.41248044010638
80-81	22.058506321847286	27.77779789561226	27.768177774911173	22.395518007629274
82-83	22.076050889740053	27.79224751485207	27.84580837805389	22.285893217353998
84-85	22.196461745095863	27.806817583028664	27.754936930391917	22.241783741483555
86-87	22.04307895622158	27.78117542311451	27.861471859013342	22.31427376165056
88-89	22.02558102992808	27.74259866911617	27.871650449838558	22.36016985111719
90-91	22.07977353658861	27.768226222642074	27.874052226487954	22.27794801428136
92-93	22.08006753425551	27.74629277194865	27.936741093782796	22.236898600013046
94-95	21.920076719536176	27.907969474358378	27.954424295063383	22.217529511042063
96-97	22.015356800105966	27.99074772987308	27.997285534476745	21.99660993554421
98-99	23.014828408767613	29.87714265652357	28.20536717910248	18.902661755606335
100	22.111863067894564	30.814264884065818	21.717130008483547	25.356742039556075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2879.0
1	2243.5
2	2270.5
3	3012.5
4	3833.5
5	6336.0
6	8598.5
7	10338.0
8	11621.5
9	11517.5
10	10081.0
11	8205.0
12	8223.0
13	10862.5
14	18801.0
15	30511.5
16	40157.5
17	45797.5
18	47790.0
19	46654.5
20	43596.0
21	40457.5
22	39834.5
23	43810.5
24	52832.0
25	67123.5
26	85911.5
27	106565.5
28	127076.5
29	149410.5
30	171243.0
31	191668.5
32	213040.0
33	233826.5
34	253797.5
35	271539.5
36	291474.0
37	311828.5
38	326270.0
39	337112.5
40	348954.0
41	359914.0
42	367034.5
43	376309.5
44	390924.0
45	406501.5
46	408539.0
47	403929.5
48	396755.0
49	383734.0
50	373687.0
51	362792.5
52	347631.0
53	327580.5
54	306928.5
55	285878.0
56	263473.5
57	238917.5
58	213605.0
59	186679.5
60	156037.5
61	127681.5
62	102607.0
63	80306.5
64	61706.5
65	46863.0
66	35241.5
67	27066.5
68	21069.0
69	16198.5
70	12540.0
71	9708.5
72	7437.0
73	5573.0
74	4170.0
75	3134.0
76	2361.0
77	1760.5
78	1307.5
79	991.0
80	744.5
81	545.0
82	420.5
83	310.5
84	226.5
85	155.0
86	111.0
87	90.0
88	60.5
89	36.5
90	26.0
91	17.0
92	12.0
93	7.5
94	3.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006654091615984311
3	1.7199590606744583E-4
4	0.0
5	0.010953989267670456
6	0.013017940140479805
7	0.0
8	0.0
9	0.0
10-11	5.91235927106845E-5
12-13	2.6874360323038412E-5
14-15	5.374872064607682E-6
16-17	2.5261898703656107E-4
18-19	0.0010857241570507517
20-21	0.0
22-23	1.1289628207813454E-4
24-25	0.0
26-27	5.379987726096002E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	8.679224383282794E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.341669499195688E-5
44-45	0.0
46-47	5.682681561960038E-6
48-49	0.0
50-51	0.006445765117826209
52-53	7.015428564346329E-5
54-55	1.1793244841148236E-5
56-57	0.0
58-59	0.0
60-61	4.860030218452891E-5
62-63	0.0010496559706518647
64-65	0.0013958677104531135
66-67	9.982553134022557E-4
68-69	0.01000062241636018
70-71	8.24438309535621E-4
72-73	3.9756704609770344E-4
74-75	3.0311303012409775E-4
76-77	7.999014521410963E-5
78-79	0.0
80-81	8.198959784307232E-5
82-83	0.0
84-85	0.0
86-87	1.1358337150799459E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	329.0
20-21	1177.0
22-23	2224.0
24-25	3804.0
26-27	6099.0
28-29	10072.0
30-31	23046.0
32-33	29347.0
34-35	40269.0
36-37	57323.0
38-39	64782.0
40-41	68202.0
42-43	78638.0
44-45	94413.0
46-47	93574.0
48-49	83897.0
50-51	75236.0
52-53	72093.0
54-55	76830.0
56-57	81356.0
58-59	86836.0
60-61	86336.0
62-63	84526.0
64-65	93421.0
66-67	98890.0
68-69	102480.0
70-71	94870.0
72-73	83510.0
74-75	85941.0
76-77	89493.0
78-79	91946.0
80-81	93608.0
82-83	89924.0
84-85	91229.0
86-87	95720.0
88-89	100325.0
90-91	104522.0
92-93	111644.0
94-95	137602.0
96-97	331380.0
98-99	724474.0
100-101	5461159.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.84953520229996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.88096755829467	73.63746850535416
2	7.423377231661066	12.30046706548911
3	1.9337941873095583	4.806418487865189
4	0.7497355818693991	2.4846097793002255
5	0.3575529678370914	1.4811548597752968
6	0.19731416859258005	0.9808432294034106
7	0.12786051995511638	0.7415229254304054
8	0.0852873573982132	0.5652814335259523
9	0.05227687287569389	0.3898003157622865
>10	0.18803274134587505	2.3650626689854333
>50	0.0033457637665806816	0.1726960744222028
>100	4.3147869388401427E-4	0.058681249644670114
>500	2.3570400287855372E-5	0.015993405041640183
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.0749744129215364E-5	0.0	0.0	1.0749744129215364E-5	0.0
3	1.0749744129215364E-5	0.0	0.0	2.1499488258430728E-5	0.0
4	1.0749744129215364E-5	7.524820890450755E-5	0.0	3.2249232387646096E-5	0.0
5	1.0749744129215364E-5	7.524820890450755E-5	0.0	3.2249232387646096E-5	0.0
6	1.0749744129215364E-5	7.524820890450755E-5	0.0	3.2249232387646096E-5	0.0
7	2.1499488258430728E-5	7.524820890450755E-5	0.0	3.2249232387646096E-5	0.0
8	2.1499488258430728E-5	7.524820890450755E-5	0.0	3.2249232387646096E-5	0.0
9	4.2998976516861456E-5	1.0749744129215365E-4	0.0	4.2998976516861456E-5	0.0
10-11	4.2998976516861456E-5	1.0749744129215365E-4	0.0	5.912359271068451E-5	0.0
12-13	5.3748720646076824E-5	1.0749744129215365E-4	0.0	6.449846477529219E-5	0.0
14-15	5.3748720646076824E-5	1.3974667367979974E-4	0.0	7.524820890450755E-5	0.0
16-17	6.449846477529219E-5	1.6124616193823047E-4	0.0	8.599795303372291E-5	0.0
18-19	6.449846477529219E-5	1.7199590606744583E-4	0.0	9.674769716293827E-5	0.0
20-21	6.449846477529219E-5	1.827456501966612E-4	0.0	1.0212256922754597E-4	0.0
22-23	6.449846477529219E-5	2.0962001051969963E-4	0.0	1.0749744129215365E-4	0.0
24-25	6.449846477529219E-5	2.2036975464891496E-4	0.0	1.236220574859767E-4	0.0
26-27	6.449846477529219E-5	2.3111949877813035E-4	0.0	2.6874360323038415E-4	0.0
28-29	6.449846477529219E-5	2.3649437084273802E-4	0.0	9.728518436939905E-4	0.0
30-31	6.449846477529219E-5	2.687436032303841E-4	0.0	0.0035796647950287162	0.0
32-33	6.449846477529219E-5	2.687436032303841E-4	0.0	0.009389901496869621	0.0
34-35	6.449846477529219E-5	2.902430914888148E-4	0.0	0.017479083954104184	0.0
36-37	6.449846477529219E-5	3.278671959410686E-4	0.0	0.030647520512393004	0.0
38-39	6.449846477529219E-5	3.5474155626410704E-4	0.0	0.05838723523783325	0.0
40-41	6.449846477529219E-5	3.7624104452253776E-4	0.0	0.0855679632685543	0.0
42-43	6.449846477529219E-5	3.9236566071636084E-4	0.0	0.10417039548416149	0.0
44-45	6.449846477529219E-5	4.407395092978299E-4	0.0	0.12834119515870224	0.0
46-47	9.674769716293827E-5	4.837384858146914E-4	0.0	0.1525281194494368	0.0
48-49	9.674769716293827E-5	5.052379740731221E-4	0.0	0.17193678247473515	0.0
50-51	9.674769716293827E-5	5.267374623315528E-4	0.0	0.18976523311303883	0.0
52-53	9.674769716293827E-5	5.267374623315528E-4	0.0	0.2389829366086514	0.0
54-55	9.674769716293827E-5	5.267374623315528E-4	0.0	0.2940108768061048	0.0
56-57	1.0749744129215363E-4	5.374872064607682E-4	0.0	0.3557466573401886	0.0
58-59	1.1824718542136901E-4	5.374872064607682E-4	0.0	0.39692355222714815	0.0
60-61	1.1824718542136901E-4	5.42862078525376E-4	0.0	0.41071547394493146	0.0
62-63	1.1824718542136901E-4	5.643615667838067E-4	0.0	0.4275011994027012	0.0
64-65	1.2899692955058438E-4	6.449846477529219E-4	0.0	0.43577850238219706	0.0
66-67	1.451215457444074E-4	6.557343918821372E-4	0.0	0.442190724755274	0.0
68-69	1.504964178090151E-4	6.664841360113525E-4	0.0	0.44855457327976955	0.0
70-71	1.504964178090151E-4	6.772338801405679E-4	0.0	0.45447230742290257	0.0
72-73	1.504964178090151E-4	6.826087522051757E-4	0.0	0.4589764502130438	0.0
74-75	1.504964178090151E-4	6.987333683989987E-4	0.0	0.46257761449633095	0.0
76-77	1.558712898736228E-4	7.25607728722037E-4	0.0	0.46586166132780626	0.0
78-79	1.6124616193823047E-4	7.309826007866447E-4	0.0	0.4680599840022308	0.0
80-81	1.6124616193823047E-4	7.363574728512525E-4	0.0	0.4687210932661775	0.0
82-83	1.6124616193823047E-4	7.417323449158601E-4	0.0	0.4695541984361917	0.0
84-85	1.6124616193823047E-4	7.417323449158601E-4	0.0	0.4697530687025822	0.0
86-87	1.7199590606744583E-4	7.524820890450755E-4	0.0	0.4698390666556159	0.0
88	1.7199590606744583E-4	7.632318331742909E-4	0.0	0.46984981639974516	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11290	0.0	51.0548	1
GTATCAA	20715	0.0	36.199596	1
TCAACGC	25540	0.0	28.590204	4
ATCAACG	25545	0.0	28.584608	3
CAACGCA	26135	0.0	27.939306	5
TATCAAC	26925	0.0	27.60211	2
AACGCAG	26980	0.0	27.153416	6
CTTATAC	11615	0.0	25.94671	1
ACGCAGA	30925	0.0	23.64659	7
CGCAGAG	31365	0.0	23.301542	8
TATACAC	14790	0.0	22.806076	3
GTGGTAT	5570	0.0	21.162167	1
GCAGAGT	37065	0.0	19.718142	9
TTATACA	15980	0.0	19.670134	2
TGGTATC	5510	0.0	19.34438	2
GAGTACT	22330	0.0	19.069002	12-13
GTACATG	25110	0.0	18.460823	1
AGAGTAC	33760	0.0	18.16418	10-11
CAGAGTA	36035	0.0	18.014696	10-11
TACACAT	18560	0.0	17.903355	5
>>END_MODULE