FastQCFastQC Report
Fri 27 May 2016
SRR2138952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8574569
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104300.051.787851
GTATCAA190000.035.2260321
TCAACGC225100.029.207384
ATCAACG225450.029.1247233
CAACGCA229450.028.5803285
TATCAAC237200.027.7730922
AACGCAG240500.027.299616
ACGCAGA273700.023.9247257
CGCAGAG284400.022.9802448
GTGGTAT50500.020.7422121
GCAGAGT333150.019.6301569
GTACATG226750.019.6284281
TGGTATC48550.019.4091242
TACATGG225200.019.2404822
GAGTACT206800.019.10653712-13
ACATGGG226450.018.5939183
CAGAGTA323600.018.29255310-11
AGAGTAC302650.017.72450810-11
GTACTTT232250.017.00507414-15
AGTACTT223600.016.3167412-13
Produced by FastQC (version 0.11.5)