##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8574569
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44981596159527	34.0	31.0	34.0	31.0	34.0
2	32.579464343922126	34.0	31.0	34.0	31.0	34.0
3	32.623710416232	34.0	31.0	34.0	31.0	34.0
4	36.15109330859662	37.0	35.0	37.0	35.0	37.0
5	36.06343105991683	37.0	35.0	37.0	35.0	37.0
6	36.07592090051406	37.0	35.0	37.0	35.0	37.0
7	36.03517564556306	37.0	35.0	37.0	35.0	37.0
8	36.032780423132635	37.0	35.0	37.0	35.0	37.0
9	37.760414196911825	39.0	38.0	39.0	35.0	39.0
10-11	37.69187156812197	39.0	38.0	39.0	35.0	39.0
12-13	37.672694685878675	39.0	37.0	39.0	35.0	39.0
14-15	39.09877015392844	41.0	38.0	41.0	36.0	41.0
16-17	39.07343628583547	41.0	38.0	41.0	35.5	41.0
18-19	39.080559034512405	41.0	38.5	41.0	35.0	41.0
20-21	39.06395644150228	41.0	39.0	41.0	35.0	41.0
22-23	39.04623050210463	41.0	39.0	41.0	35.0	41.0
24-25	39.00498975293269	41.0	39.0	41.0	35.0	41.0
26-27	38.94769962770542	41.0	38.0	41.0	35.0	41.0
28-29	38.88857783743765	40.5	38.0	41.0	35.0	41.0
30-31	38.81147783399122	40.0	38.0	41.0	35.0	41.0
32-33	38.71853270482869	40.0	38.0	41.0	34.0	41.0
34-35	38.64283962537445	40.0	38.0	41.0	34.0	41.0
36-37	38.62527702059434	40.0	38.0	41.0	34.0	41.0
38-39	38.58017438485193	40.0	38.0	41.0	34.0	41.0
40-41	38.5642859284182	40.0	38.0	41.0	34.0	41.0
42-43	38.51577822562267	40.0	38.0	41.0	34.0	41.0
44-45	38.43042626572645	40.0	38.0	41.0	33.5	41.0
46-47	38.383359973568325	40.0	38.0	41.0	33.0	41.0
48-49	38.332747316417226	40.0	38.0	41.0	33.0	41.0
50-51	38.27875951984548	40.0	38.0	41.0	33.0	41.0
52-53	38.15616341738579	40.0	37.0	41.0	33.0	41.0
54-55	38.03321208239234	40.0	37.0	41.0	33.0	41.0
56-57	37.915766446498914	40.0	37.0	41.0	33.0	41.0
58-59	37.80108830640253	40.0	36.5	41.0	33.0	41.0
60-61	37.665877251630434	39.5	36.0	41.0	32.5	41.0
62-63	37.45136153043775	39.0	36.0	41.0	32.0	41.0
64-65	37.236571828220576	39.0	35.0	41.0	32.0	41.0
66-67	37.00497688338392	38.5	35.0	41.0	32.0	41.0
68-69	36.67508328382182	37.5	35.0	40.0	31.0	41.0
70-71	36.34559938574188	37.0	35.0	40.0	31.0	41.0
72-73	36.036976446732986	37.0	35.0	39.0	31.0	41.0
74-75	35.76627518580872	36.0	35.0	39.0	31.0	41.0
76-77	35.47445218022909	36.0	35.0	39.0	31.0	40.5
78-79	35.198247560245974	35.0	34.0	37.0	31.0	39.5
80-81	34.92096060019023	35.0	34.0	37.0	31.0	39.0
82-83	34.626368877804445	35.0	34.0	36.5	31.0	39.0
84-85	34.39415754959299	35.0	34.0	36.0	31.0	37.5
86-87	34.23018675610726	35.0	34.0	36.0	31.0	37.0
88-89	34.08906997282251	35.0	34.0	35.5	31.0	37.0
90-91	33.955010101496235	35.0	34.0	35.0	31.0	36.0
92-93	33.89879653317125	35.0	34.0	35.0	31.0	36.0
94-95	33.84725251451526	35.0	34.0	35.0	31.0	36.0
96-97	33.84306445995883	35.0	34.0	35.0	31.0	36.0
98-99	33.97914567523085	35.0	34.0	35.0	31.0	36.0
100	31.2227492593634	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	19.0
19	43.0
20	85.0
21	274.0
22	1039.0
23	3485.0
24	9313.0
25	19819.0
26	35860.0
27	57313.0
28	83498.0
29	114210.0
30	147206.0
31	185939.0
32	231766.0
33	290672.0
34	378469.0
35	522995.0
36	768159.0
37	1437474.0
38	2367478.0
39	1742605.0
40	176847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.524882358518546	20.603519547163245	14.657354789494375	24.214243304823835
2	14.82477731524268	21.31082514073938	40.97983859116464	22.884558952853297
3	17.959025731023615	26.08922308710706	30.813166682061055	25.13858449980827
4	12.670666012484125	18.362194064797894	38.66352932724666	30.30361059547133
5	13.047943931261699	37.84650332607502	35.84100249847818	13.2645502441851
6	29.742316590472623	38.27908189241157	18.64677281928453	13.331828697831282
7	26.024841598452355	32.90666854508956	23.458240291727783	17.61024956473031
8	24.16038637044031	36.94852767526858	21.217486266656667	17.673599687634443
9	25.258808926722732	16.93932371411321	21.542540505534447	36.25932685362961
10-11	23.96439936020078	26.93136036638593	29.130999648378662	19.97324062503462
12-13	24.737499803197014	24.51205979545318	29.22050273104233	21.529937670307472
14-15	21.93073878070235	25.89197647578143	26.872060894033428	25.30522384948279
16-17	21.521263950080165	28.887699321742634	27.631382297433188	21.959654430744013
18-19	21.557339982512083	28.02627094256599	29.352524362229016	21.063864712692908
20-21	22.30707690122212	27.26894419634175	29.262402262729236	21.16157663970689
22-23	22.0978453242162	27.108782446480127	29.0695149551769	21.72385727412677
24-25	21.900685202225695	27.234511775839444	29.130466818555266	21.73433620337959
26-27	21.842485687457305	27.361219347858874	29.08215056313978	21.71414440154404
28-29	21.836267912066603	27.38376066079712	29.1182713300633	21.661700097072973
30-31	22.05711057793904	27.255349148778464	28.984650341198087	21.702889932084414
32-33	21.857710831144335	27.381308465907022	28.94716612068044	21.8138145822682
34-35	21.95677337956065	27.450507187522156	28.926629823267586	21.666089609649607
36-37	21.99973838566221	27.275534927730664	28.880473279606438	21.844253407000682
38-39	21.88042730413071	27.469017535436898	28.88402446174261	21.766530698689788
40-41	22.09028109158325	27.369124529289845	28.77372272831708	21.766871650809826
42-43	22.03644948611428	27.45149384822111	28.578034207536668	21.93402245812794
44-45	22.088719624055493	27.60656529823322	28.341270896677944	21.96344418103334
46-47	22.17748774134935	27.5625586246155	28.05866669194742	22.201286942087727
48-49	22.123791945419793	27.637479344192812	28.071072517537925	22.16765619284947
50-51	22.05417106153574	27.769156613773017	27.999891554703275	22.176780769987964
52-53	22.185507082006673	27.748336046779798	27.888472413603672	22.177684457609857
54-55	22.260858429027586	27.652195741307846	27.884093848124486	22.202851981540086
56-57	22.203890748886867	27.711304169407043	27.899570161963528	22.18523491974256
58-59	22.157358450889703	27.783850352330774	27.970913842659247	22.087877354120277
60-61	22.25842366725849	27.782235630813574	27.876724161147447	22.082616540780485
62-63	22.186460150259492	27.894632714496627	27.825553607482156	22.093353527761725
64-65	22.148453177987783	27.817732353917314	27.82529501575744	22.208519452337462
66-67	22.18507597496786	27.864091158468256	27.69380960606461	22.25702326049927
68-69	22.156867501741687	27.789889376078627	27.80886597240706	22.24437714977263
70-71	22.153270973297023	27.567668485857926	27.838965449456555	22.440095091388493
72-73	22.217259159346497	27.629601382286307	27.745399202322012	22.40774025604518
74-75	22.04584067570717	27.771411095631706	27.789234514150678	22.393513714510448
76-77	22.21607306285081	27.719230048560455	27.718916332107078	22.345780556481653
78-79	22.180588912446055	27.725411606519934	27.706891530772243	22.38710795026177
80-81	22.185447160707003	27.773873240048164	27.668271933980904	22.37240766526393
82-83	22.244056691834153	27.740952110878737	27.76838674039677	22.24660445689034
84-85	22.33221540198753	27.72194264805965	27.67621316871518	22.269628781237643
86-87	22.146548010699764	27.81183213944938	27.74931727184059	22.292302578010272
88-89	22.191723523149225	27.67285244351827	27.76449414973694	22.370929883595565
90-91	22.17527694003354	27.786004535864993	27.770095901012386	22.26862262308908
92-93	22.26078498400656	27.717887272463976	27.803931782139625	22.217395961389844
94-95	22.088957101213907	27.860718978897953	27.77938115411501	22.27094276577313
96-97	22.17512174406436	27.905980740551794	27.87221917766819	22.046678337715658
98-99	23.184326778569517	29.78310485199605	28.11419535501583	18.918373014418606
100	22.244305000817917	30.82781118331675	21.521064894455765	25.406818921409563
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2074.0
1	1684.0
2	1921.5
3	2609.5
4	3253.5
5	5364.5
6	7385.5
7	9116.5
8	10333.5
9	10166.0
10	8716.5
11	6913.0
12	7126.0
13	9790.0
14	16915.5
15	27245.0
16	35981.5
17	41225.0
18	43008.0
19	42042.5
20	39515.5
21	36817.0
22	36544.5
23	40483.5
24	48577.5
25	60874.0
26	77371.0
27	96244.5
28	114155.5
29	132483.0
30	150594.0
31	168576.0
32	189141.5
33	209426.5
34	228876.5
35	247464.0
36	268616.0
37	286732.5
38	297326.0
39	308042.0
40	321367.5
41	333030.0
42	339043.0
43	345216.5
44	351795.5
45	356732.0
46	356699.5
47	353983.5
48	354942.0
49	354061.5
50	348698.5
51	339130.5
52	324765.0
53	306483.0
54	288539.0
55	269171.0
56	247571.5
57	225709.0
58	203592.0
59	179101.0
60	149313.5
61	120733.0
62	96215.5
63	74788.0
64	57540.5
65	43965.0
66	33561.5
67	26190.5
68	20385.5
69	15658.0
70	11994.5
71	9112.0
72	6901.5
73	5210.0
74	3963.0
75	3032.5
76	2285.5
77	1716.0
78	1289.5
79	970.5
80	731.5
81	529.5
82	404.5
83	300.0
84	226.0
85	172.0
86	132.0
87	106.5
88	75.0
89	46.5
90	28.5
91	18.0
92	11.5
93	7.5
94	5.0
95	5.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006589252474380927
3	1.9826069391942617E-4
4	0.0
5	0.011079274071967932
6	0.012408786960604084
7	0.0
8	0.0
9	0.0
10-11	4.664957503986498E-5
12-13	4.664957503986498E-5
14-15	1.7493590639949367E-5
16-17	2.5074146583927424E-4
18-19	8.921731226374178E-4
20-21	0.0
22-23	1.4580864306977352E-4
24-25	0.0
26-27	5.836612780139174E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.294997862585863E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.621887938062819E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.006105950860964174
52-53	1.1392158473531777E-4
54-55	4.468907670643803E-5
56-57	0.0
58-59	6.506329454888657E-6
60-61	8.53910215807415E-5
62-63	9.613565815432929E-4
64-65	0.001392045810086001
66-67	9.667696265051183E-4
68-69	0.009647954213487771
70-71	8.979145726838362E-4
72-73	5.235270983908453E-4
74-75	4.372960490019846E-4
76-77	9.981877188972194E-5
78-79	0.0
80-81	1.3865123580576218E-4
82-83	0.0
84-85	7.472136589461313E-6
86-87	2.0422238092738441E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	298.0
20-21	969.0
22-23	2019.0
24-25	3561.0
26-27	5214.0
28-29	8955.0
30-31	20645.0
32-33	26242.0
34-35	35779.0
36-37	51573.0
38-39	58775.0
40-41	60811.0
42-43	71195.0
44-45	84892.0
46-47	83719.0
48-49	74925.0
50-51	68353.0
52-53	67176.0
54-55	71821.0
56-57	74517.0
58-59	73014.0
60-61	71252.0
62-63	69706.0
64-65	73918.0
66-67	78071.0
68-69	78579.0
70-71	77345.0
72-73	73619.0
74-75	75400.0
76-77	78845.0
78-79	81001.0
80-81	82036.0
82-83	79196.0
84-85	79884.0
86-87	84784.0
88-89	89747.0
90-91	93394.0
92-93	99536.0
94-95	124974.0
96-97	307275.0
98-99	678217.0
100-101	5153337.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.65184556470406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.15848550114431	77.22240676491624
2	6.916500876962603	11.848221299234822
3	1.637777031030516	4.208358759937359
4	0.5719289419410212	1.9594707763646777
5	0.27578666539631247	1.1810818436664836
6	0.1469476859531018	0.7551804302007425
7	0.08509842351433086	0.5102185920054474
8	0.05520197236584804	0.37825206495573427
9	0.03315217440647803	0.2555590430158012
>10	0.11722353697251403	1.5031386098990231
>50	0.0014410735800797986	0.07837105181018064
>100	3.912380523347052E-4	0.062286892422047886
>500	6.487868069531305E-5	0.037453871571508784
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1662393759966244E-5	0.0	2.3324787519932488E-5	0.0
3	0.0	1.1662393759966244E-5	0.0	2.3324787519932488E-5	0.0
4	0.0	3.4987181279898735E-5	0.0	2.3324787519932488E-5	0.0
5	0.0	3.4987181279898735E-5	0.0	2.3324787519932488E-5	0.0
6	2.3324787519932488E-5	3.4987181279898735E-5	0.0	3.4987181279898735E-5	1.1662393759966244E-5
7	2.3324787519932488E-5	3.4987181279898735E-5	0.0	4.6649575039864975E-5	1.1662393759966244E-5
8	2.3324787519932488E-5	3.4987181279898735E-5	0.0	4.6649575039864975E-5	1.1662393759966244E-5
9	2.3324787519932488E-5	3.4987181279898735E-5	0.0	5.831196879983122E-5	2.3324787519932488E-5
10-11	2.3324787519932488E-5	3.4987181279898735E-5	0.0	5.831196879983122E-5	2.3324787519932488E-5
12-13	2.3324787519932488E-5	3.4987181279898735E-5	0.0	5.831196879983122E-5	2.915598439991561E-5
14-15	2.3324787519932488E-5	7.580555943978059E-5	0.0	5.831196879983122E-5	5.2480771919848095E-5
16-17	6.997436255979747E-5	8.163675631976371E-5	0.0	5.831196879983122E-5	5.831196879983122E-5
18-19	6.997436255979747E-5	8.746795319974683E-5	0.0	1.0496154383969619E-4	5.831196879983122E-5
20-21	6.997436255979747E-5	1.049615438396962E-4	0.0	1.282863313596287E-4	5.831196879983122E-5
22-23	8.163675631976371E-5	1.1662393759966244E-4	0.0	1.3994872511959494E-4	5.831196879983122E-5
24-25	8.163675631976371E-5	1.1662393759966244E-4	0.0	1.3994872511959494E-4	6.997436255979747E-5
26-27	8.163675631976371E-5	1.1662393759966244E-4	0.0	1.6327351263952742E-4	8.163675631976371E-5
28-29	9.329915007972995E-5	1.1662393759966244E-4	0.0	3.0905343463910547E-4	8.746795319974683E-5
30-31	9.329915007972995E-5	1.3994872511959494E-4	0.0	0.0010729402259168944	9.913034695971308E-5
32-33	9.329915007972995E-5	1.3994872511959494E-4	0.0	0.002513245855272726	1.049615438396962E-4
34-35	9.329915007972995E-5	1.6910470951951053E-4	0.0	0.004379228856867325	1.049615438396962E-4
36-37	9.329915007972995E-5	1.92429497039443E-4	0.0	0.007580555943978059	1.1079274071967932E-4
38-39	9.329915007972995E-5	2.0409189079940927E-4	0.0	0.012525410898203746	1.3994872511959494E-4
40-41	1.049615438396962E-4	2.2158548143935865E-4	0.0	0.0178959432246682	1.3994872511959494E-4
42-43	1.049615438396962E-4	2.2741667831934176E-4	0.0	0.02287578536017379	1.3994872511959494E-4
44-45	1.049615438396962E-4	2.565726627192574E-4	0.0	0.02863117668071713	1.4577992199957805E-4
46-47	1.049615438396962E-4	2.798974502391899E-4	0.0	0.034783089389099325	1.5161111887956118E-4
48-49	1.049615438396962E-4	2.85728647119173E-4	0.0	0.04084753414428177	1.6327351263952742E-4
50-51	1.049615438396962E-4	3.2071582839907173E-4	0.0	0.04659709426794513	1.6327351263952742E-4
52-53	1.049615438396962E-4	3.382094190390211E-4	0.0	0.052043432153849364	1.6327351263952742E-4
54-55	1.049615438396962E-4	3.382094190390211E-4	0.0	0.05767053714303308	1.6327351263952742E-4
56-57	1.049615438396962E-4	3.382094190390211E-4	0.0	0.06415482807357431	1.6327351263952742E-4
58-59	1.049615438396962E-4	3.382094190390211E-4	0.0	0.07060996301971563	1.6910470951951053E-4
60-61	1.1662393759966246E-4	3.440406159190042E-4	0.0	0.07665108298737813	1.7493590639949366E-4
62-63	1.7493590639949366E-4	3.498718127989873E-4	0.0	0.08258141021432097	1.865983001594599E-4
64-65	1.7493590639949366E-4	3.965213878388523E-4	0.0	0.08778283783126592	1.865983001594599E-4
66-67	1.7493590639949366E-4	4.198461753587848E-4	0.0	0.09384728258644837	1.865983001594599E-4
68-69	1.7493590639949366E-4	4.198461753587848E-4	0.0	0.0996376610882716	1.865983001594599E-4
70-71	1.9826069391942614E-4	4.3150856911875103E-4	0.0	0.10468164638945701	1.865983001594599E-4
72-73	1.9826069391942614E-4	4.3150856911875103E-4	0.0	0.10864686026784554	1.9826069391942614E-4
74-75	1.9826069391942614E-4	4.6066455351866667E-4	0.0	0.11176071940175653	1.9826069391942614E-4
76-77	1.9826069391942614E-4	5.014829316785485E-4	0.0	0.11484542255126759	1.9826069391942614E-4
78-79	1.9826069391942614E-4	5.24807719198481E-4	0.0	0.11690383504990162	1.9826069391942614E-4
80-81	1.9826069391942614E-4	5.24807719198481E-4	0.0	0.11741114917846016	2.0992308767939238E-4
82-83	1.9826069391942614E-4	5.306389160784641E-4	0.0	0.11808173681965822	2.2158548143935865E-4
84-85	1.9826069391942614E-4	5.364701129584472E-4	0.0	0.11827416631669767	2.2158548143935865E-4
86-87	1.9826069391942614E-4	5.364701129584472E-4	0.0	0.11836163426989742	2.2741667831934176E-4
88	1.9826069391942614E-4	5.71457294238346E-4	0.0	0.11836163426989742	2.4491026895929113E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10430	0.0	51.78785	1
GTATCAA	19000	0.0	35.226032	1
TCAACGC	22510	0.0	29.20738	4
ATCAACG	22545	0.0	29.124723	3
CAACGCA	22945	0.0	28.580328	5
TATCAAC	23720	0.0	27.773092	2
AACGCAG	24050	0.0	27.29961	6
ACGCAGA	27370	0.0	23.924725	7
CGCAGAG	28440	0.0	22.980244	8
GTGGTAT	5050	0.0	20.742212	1
GCAGAGT	33315	0.0	19.630156	9
GTACATG	22675	0.0	19.628428	1
TGGTATC	4855	0.0	19.409124	2
TACATGG	22520	0.0	19.240482	2
GAGTACT	20680	0.0	19.106537	12-13
ACATGGG	22645	0.0	18.593918	3
CAGAGTA	32360	0.0	18.292553	10-11
AGAGTAC	30265	0.0	17.724508	10-11
GTACTTT	23225	0.0	17.005074	14-15
AGTACTT	22360	0.0	16.31674	12-13
>>END_MODULE