FastQCFastQC Report
Fri 27 May 2016
SRR2138951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13078504
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC195050.053.2331621
GGTATCA200350.050.1672131
TATACAC251150.043.4719243
TTATACA291150.036.903032
GTATCAA350000.036.1425631
ACATCTC348700.030.0184388
ACACATC350550.030.005316
TACACAT370750.029.7617475
ATCAACG431450.028.6921753
ATACACA409150.028.044014
CAACGCA442850.027.9065935
CACATCT377300.027.797997
TCAACGC446650.027.752824
TATCAAC459100.027.2823982
AACGCAG466050.026.5949256
CATCTCC398500.026.2159
ACGCAGA517300.023.9174277
CGCAGAG527350.023.4694928
GCAGAGT599750.020.6086449
TGGTATC88350.019.9708652
Produced by FastQC (version 0.11.5)