FastQCFastQC Report
Fri 27 May 2016
SRR2138950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9753691
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112400.048.3881231
GTATCAA195200.033.0050851
TCAACGC231150.027.179664
ATCAACG230900.027.1545263
CAACGCA236750.026.4125965
TATCAAC246150.025.7980732
AACGCAG245250.025.528416
ACGCAGA282900.022.0238767
CGCAGAG288900.021.5664758
GTGGTAT62450.019.5702291
TGGTATC58150.018.9228652
GTACATG237250.018.6227951
TACATGG236350.018.3205432
GCAGAGT340650.018.2162449
ACATGGG232500.017.8459783
GAGTACT221100.017.49853312-13
CAGAGTA335450.017.20947810-11
AGAGTAC311950.016.65532110-11
CATGGGG173900.016.1318154
GTACTTT254750.015.22909214-15
Produced by FastQC (version 0.11.5)