##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9753691
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43031330395847	34.0	31.0	34.0	31.0	34.0
2	32.56211387053373	34.0	31.0	34.0	31.0	34.0
3	32.61289659473527	34.0	31.0	34.0	31.0	34.0
4	36.13648474203253	37.0	35.0	37.0	35.0	37.0
5	36.04591328554493	37.0	35.0	37.0	35.0	37.0
6	36.058307055247084	37.0	35.0	37.0	35.0	37.0
7	36.016993669370905	37.0	35.0	37.0	35.0	37.0
8	36.01240258687711	37.0	35.0	37.0	35.0	37.0
9	37.735753060046704	39.0	38.0	39.0	35.0	39.0
10-11	37.66994981694622	39.0	37.0	39.0	35.0	39.0
12-13	37.65188491208097	39.0	37.0	39.0	35.0	39.0
14-15	39.072677615069004	41.0	38.0	41.0	35.0	41.0
16-17	39.04831817001379	41.0	38.0	41.0	35.0	41.0
18-19	39.05408101404893	41.0	38.5	41.0	35.0	41.0
20-21	39.04410622846467	41.0	39.0	41.0	35.0	41.0
22-23	39.0273706538114	41.0	39.0	41.0	35.0	41.0
24-25	38.99002882713698	41.0	39.0	41.0	35.0	41.0
26-27	38.93041009392863	41.0	38.0	41.0	35.0	41.0
28-29	38.87266581174781	40.0	38.0	41.0	35.0	41.0
30-31	38.79695751678916	40.0	38.0	41.0	34.5	41.0
32-33	38.70766954134217	40.0	38.0	41.0	34.0	41.0
34-35	38.63123235521127	40.0	38.0	41.0	34.0	41.0
36-37	38.6099341401329	40.0	38.0	41.0	34.0	41.0
38-39	38.55969459244395	40.0	38.0	41.0	34.0	41.0
40-41	38.55135390093522	40.0	38.0	41.0	34.0	41.0
42-43	38.50755244091704	40.0	38.0	41.0	34.0	41.0
44-45	38.42969143491291	40.0	38.0	41.0	33.5	41.0
46-47	38.38916555873288	40.0	38.0	41.0	33.0	41.0
48-49	38.348626276710604	40.0	38.0	41.0	33.0	41.0
50-51	38.290303605712495	40.0	38.0	41.0	33.0	41.0
52-53	38.174711692273775	40.0	37.5	41.0	33.0	41.0
54-55	38.06244909697954	40.0	37.0	41.0	33.0	41.0
56-57	37.94784685651746	40.0	37.0	41.0	33.0	41.0
58-59	37.84859351759907	40.0	37.0	41.0	33.0	41.0
60-61	37.72332347208309	40.0	36.0	41.0	32.5	41.0
62-63	37.53634152694691	39.0	36.0	41.0	32.0	41.0
64-65	37.34773042684337	39.0	35.5	41.0	32.0	41.0
66-67	37.134139459179124	39.0	35.0	41.0	32.0	41.0
68-69	36.822802133203396	38.0	35.0	40.5	31.0	41.0
70-71	36.51322698374469	37.5	35.0	40.0	31.0	41.0
72-73	36.21628326028494	37.0	35.0	39.5	31.0	41.0
74-75	35.9498011274775	36.5	35.0	39.0	31.0	41.0
76-77	35.65067787673519	36.0	35.0	39.0	31.0	41.0
78-79	35.36458714082819	36.0	34.5	38.0	31.0	39.5
80-81	35.0721663529003	35.0	34.0	37.0	31.0	39.0
82-83	34.75807637490361	35.0	34.0	37.0	31.0	39.0
84-85	34.50781918804839	35.0	34.0	36.0	31.0	38.0
86-87	34.32390757518502	35.0	34.0	36.0	31.0	37.0
88-89	34.16372281692278	35.0	34.0	36.0	31.0	37.0
90-91	34.016552351757454	35.0	34.0	35.5	31.0	36.5
92-93	33.95107658903514	35.0	34.0	35.0	31.0	36.0
94-95	33.885691565198414	35.0	34.0	35.0	31.0	36.0
96-97	33.87379467063411	35.0	34.0	35.0	31.0	36.0
98-99	34.01039039350283	35.0	34.0	35.0	31.0	36.0
100	31.255959451509376	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	24.0
19	72.0
20	97.0
21	328.0
22	1270.0
23	4286.0
24	11033.0
25	23336.0
26	41788.0
27	66232.0
28	97247.0
29	129684.0
30	167068.0
31	210319.0
32	260841.0
33	326549.0
34	425049.0
35	581800.0
36	849244.0
37	1557358.0
38	2654944.0
39	2141935.0
40	203185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.676036487110366	21.255573915556685	14.855360909013829	24.21302868831912
2	14.319391576502797	21.4020296163005	42.005234246635034	22.273344560561668
3	17.86405087393699	26.427169320127238	30.885497757871438	24.82328204806433
4	12.39828081492432	18.74368380134249	39.127290376535406	29.73074500719779
5	12.721474182690079	38.01725159762459	36.27092825669944	12.990345962985886
6	29.064223118517994	38.82441244462309	19.066012406068815	13.0453520307901
7	25.54840008772064	33.00958580705499	23.827369556817004	17.61464454840737
8	23.378729139563678	37.9561337343986	21.50257784463338	17.16255928140434
9	24.710758214505667	17.442217515400067	22.05419466333309	35.792829606761174
10-11	23.565768148417295	27.340340392270086	29.441996709347272	19.651894749965347
12-13	24.33283434254144	24.75379310408464	29.59942737551451	21.31394517785941
14-15	21.41017289814558	26.261064978430472	27.226791746160085	25.101970377263864
16-17	21.068018773451115	29.39116491309551	27.94114111435519	21.599675199098186
18-19	21.071040418421507	28.51503333201929	29.592767682569203	20.821158566989993
20-21	21.788161006149505	27.664415460739168	29.56904251275717	20.978381020354156
22-23	21.532244406868145	27.720053740293565	29.36520034502651	21.38250150781178
24-25	21.395401125050185	27.82528796562281	29.45456134387308	21.32474956545393
26-27	21.375900902888535	28.028646849351446	29.296391175487273	21.299061072272746
28-29	21.26624054285012	27.98826014115003	29.357600555312718	21.387898760687133
30-31	21.664436652293062	27.8064151082921	29.32177068437966	21.207377555035176
32-33	21.45488302529026	27.889179275266084	29.303205925773117	21.352731773670534
34-35	21.497210639893698	28.01106769064233	29.321864822438027	21.169856847025947
36-37	21.52004589314031	27.91176223957526	29.197918745254576	21.37027312202985
38-39	21.331082398879644	27.914358460892657	29.31151150657178	21.44304763365591
40-41	21.5859743194486	27.822476783327193	29.220342032521263	21.371206864702945
42-43	21.514822747673694	27.85523402582189	29.07310368552471	21.556839540979706
44-45	21.554358558847287	28.00923950001754	28.885631829700454	21.550770111434723
46-47	21.62618855253956	27.982930235386615	28.576788421358977	21.81409279071485
48-49	21.637862650838528	28.034078049286858	28.5443248478927	21.78373445198191
50-51	21.544948891291646	28.197780650033128	28.487609101126317	21.76966135754891
52-53	21.583813668559674	28.195733167817778	28.457549397631677	21.762903765990878
54-55	21.645419811655387	28.146951814812855	28.41877680724011	21.78885156629165
56-57	21.65778385863639	28.196839937068564	28.34908663338396	21.796289570911085
58-59	21.52661192200169	28.331596201859643	28.486791917398886	21.654999958739786
60-61	21.703203298699215	28.407379700008928	28.25275389774905	21.636663103542805
62-63	21.55693486304368	28.47173508118418	28.25343681431196	21.71789324146018
64-65	21.525294452471133	28.452641415040464	28.23685602839361	21.785208104094796
66-67	21.58896612845947	28.53523874214112	28.083060509135628	21.792734620263776
68-69	21.58689466710402	28.400146880545734	28.119793371313044	21.8931650810372
70-71	21.577536582579196	28.133099901318154	28.251705898584618	22.037657617518033
72-73	21.69568268363457	28.10931432539725	28.169620526342616	22.02538246462557
74-75	21.631755166686172	28.220185873698522	28.152531483229282	21.99552747638602
76-77	21.697155625658855	28.225334017448716	28.04941234131989	22.02809801557254
78-79	21.67477453155006	28.20275506249604	28.08219944940481	22.040270956549087
80-81	21.708816179399008	28.230382095111484	28.060855531187094	21.999946194302414
82-83	21.708094672913177	28.24672487291451	28.1779838994001	21.867196554772217
84-85	21.87516261043513	28.200374259237428	28.087602481624607	21.83686064870284
86-87	21.70435109229163	28.293656209912786	28.13704615807865	21.864946539716932
88-89	21.732983590095888	28.277084912160415	28.137347327561713	21.85258417018198
90-91	21.748670964977716	28.3634493720454	28.098593119592426	21.789286543384456
92-93	21.831627878897642	28.28921287492328	28.192787303411293	21.68637194276779
94-95	21.669382887393297	28.40552330266297	28.2541592666197	21.670934543324034
96-97	21.705826603576458	28.455869485743197	28.373916900425012	21.464387010255333
98-99	22.699095226777832	30.304840454527255	28.636194028529776	18.35987029016514
100	21.729323359496544	31.38680797820645	22.112011832888385	24.771856829408627
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1913.0
1	1621.0
2	2055.0
3	2866.5
4	3776.5
5	6388.0
6	8589.0
7	10046.0
8	10869.0
9	10176.0
10	8569.0
11	6902.5
12	7238.5
13	10354.5
14	18548.0
15	30262.5
16	39895.5
17	45689.0
18	47865.5
19	47393.5
20	45585.5
21	43853.0
22	44658.0
23	50147.5
24	60998.5
25	77018.5
26	97758.0
27	120537.0
28	144643.5
29	172117.5
30	196998.5
31	219824.0
32	243767.5
33	266005.0
34	287354.5
35	307013.0
36	326891.5
37	346866.0
38	362435.5
39	373884.0
40	385356.5
41	396158.5
42	404263.0
43	408797.5
44	412063.5
45	418430.5
46	424905.0
47	424364.0
48	415216.0
49	400370.5
50	385015.5
51	367555.5
52	346418.5
53	324034.5
54	299524.5
55	272551.5
56	246550.0
57	220867.0
58	194038.0
59	167719.5
60	139646.0
61	112902.5
62	90111.0
63	70174.5
64	53313.0
65	40905.0
66	31611.0
67	24950.5
68	19725.0
69	14834.5
70	11140.5
71	8325.5
72	6265.0
73	4746.5
74	3563.0
75	2707.0
76	2040.0
77	1496.0
78	1078.5
79	772.5
80	552.5
81	386.5
82	290.5
83	211.5
84	156.5
85	113.5
86	84.5
87	73.5
88	53.5
89	35.0
90	23.5
91	18.0
92	15.5
93	8.5
94	3.5
95	3.0
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0063155578744497854
3	1.332828772205312E-4
4	0.0
5	0.0111444990414398
6	0.012364549994458508
7	0.0
8	0.0
9	0.0
10-11	3.075758705089181E-5
12-13	4.101011606785575E-5
14-15	5.1262645084819685E-6
16-17	2.4093443189865252E-4
18-19	8.355811148825609E-4
20-21	0.0
22-23	1.1792784169694677E-4
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.105527855109734E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.0643898374612855E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0063422436409729535
52-53	1.4520193875862508E-4
54-55	6.756348941099951E-5
56-57	0.0
58-59	5.7305851357217456E-6
60-61	1.0995497633074456E-4
62-63	0.0011335791564009096
64-65	0.0015281516209617561
66-67	0.0012103731723588688
68-69	0.00999440180883
70-71	0.0010730148828383584
72-73	5.977586883917234E-4
74-75	4.544720768309326E-4
76-77	1.1325011924293805E-4
78-79	0.0
80-81	1.6733801390630385E-4
82-83	0.0
84-85	6.586751368776335E-6
86-87	1.999358339263652E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	328.0
20-21	1155.0
22-23	2204.0
24-25	3817.0
26-27	6013.0
28-29	10707.0
30-31	26953.0
32-33	32527.0
34-35	43491.0
36-37	64540.0
38-39	72303.0
40-41	75120.0
42-43	83321.0
44-45	97457.0
46-47	97935.0
48-49	88360.0
50-51	76856.0
52-53	71634.0
54-55	74138.0
56-57	78945.0
58-59	84053.0
60-61	84374.0
62-63	81885.0
64-65	87159.0
66-67	90506.0
68-69	93683.0
70-71	89840.0
72-73	82512.0
74-75	83670.0
76-77	85987.0
78-79	90393.0
80-81	91721.0
82-83	87248.0
84-85	87866.0
86-87	91838.0
88-89	97370.0
90-91	103419.0
92-93	110602.0
94-95	139666.0
96-97	341619.0
98-99	769382.0
100-101	5871094.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.41085825929224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.43080047218932	73.76088963941537
2	7.850833096425319	13.096894532465855
3	2.0807841028785896	5.206799636201839
4	0.7722352342766663	2.576512146763294
5	0.3531112029151672	1.4726654248062596
6	0.18002514499383832	0.9009631111313748
7	0.10552163453612536	0.6161155071107113
8	0.061700152138560095	0.4117170115684963
9	0.04426931239053355	0.33232872069388114
>10	0.11871372118555502	1.4511208582184896
>50	0.001611952664998428	0.09307189508374601
>100	3.538542126345323E-4	0.05299601592650105
>500	4.0119192729158235E-5	0.02792550061412871
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0252529016963937E-5
2	2.0505058033927874E-5	5.1262645084819686E-5	0.0	0.0	1.0252529016963937E-5
3	2.0505058033927874E-5	5.1262645084819686E-5	0.0	0.0	1.0252529016963937E-5
4	2.0505058033927874E-5	6.151517410178362E-5	0.0	0.0	1.0252529016963937E-5
5	2.0505058033927874E-5	6.151517410178362E-5	0.0	0.0	1.0252529016963937E-5
6	2.0505058033927874E-5	6.151517410178362E-5	0.0	0.0	1.0252529016963937E-5
7	2.0505058033927874E-5	6.151517410178362E-5	0.0	0.0	1.0252529016963937E-5
8	2.0505058033927874E-5	6.151517410178362E-5	0.0	0.0	1.0252529016963937E-5
9	6.151517410178362E-5	8.20202321357115E-5	0.0	1.0252529016963937E-5	3.075758705089181E-5
10-11	8.20202321357115E-5	8.714649664419346E-5	0.0	5.126264508481968E-5	3.075758705089181E-5
12-13	9.227276115267543E-5	1.1277781918660331E-4	0.0	8.20202321357115E-5	3.075758705089181E-5
14-15	9.227276115267543E-5	1.8967178681383282E-4	0.0	1.2303034820356724E-4	5.1262645084819686E-5
16-17	9.227276115267543E-5	1.947980513223148E-4	0.0	1.5378793525445907E-4	5.1262645084819686E-5
18-19	1.0765155467812134E-4	2.2555563837320662E-4	0.0	1.7429299328838692E-4	5.1262645084819686E-5
20-21	1.5891419976294101E-4	2.6143948993258043E-4	0.0	1.8454552230535087E-4	5.1262645084819686E-5
22-23	1.947980513223148E-4	3.0757587050891813E-4	0.0	2.1530310935624267E-4	8.714649664419346E-5
24-25	2.0505058033927875E-4	3.127021350174001E-4	0.0	3.2295466403436403E-4	1.2303034820356724E-4
26-27	2.0505058033927875E-4	3.2295466403436403E-4	0.0	4.97247657322751E-4	1.4353540623749511E-4
28-29	2.1530310935624267E-4	3.28080928542846E-4	0.0	0.0018095713714941348	1.4353540623749511E-4
30-31	2.4093443189865252E-4	3.895961026446296E-4	0.0	0.0068896994993997655	1.486616707459771E-4
32-33	2.8707081247499023E-4	3.895961026446296E-4	0.0	0.017255006335550306	1.5378793525445907E-4
34-35	4.408587477294493E-4	4.2035368969552144E-4	0.0	0.031121551830994028	1.794192577968689E-4
36-37	4.6136380576337714E-4	4.8186886379730505E-4	0.0	0.05250320109587232	1.8454552230535087E-4
38-39	5.075001863397148E-4	5.433840378990887E-4	0.0	0.10089001179143363	1.8454552230535087E-4
40-41	5.382577733906066E-4	6.151517410178363E-4	0.0	0.14921530731289315	1.8454552230535087E-4
42-43	5.536365669160526E-4	6.151517410178363E-4	0.0	0.1822643345990764	1.8967178681383282E-4
44-45	5.536365669160526E-4	6.407830635602461E-4	0.0	0.22517116853507047	1.9992431583079677E-4
46-47	5.946466829839084E-4	6.664143861026559E-4	0.0	0.26597623402258697	2.1530310935624267E-4
48-49	6.151517410178363E-4	6.664143861026559E-4	0.0	0.2989791249281939	2.460606964071345E-4
50-51	6.356567990517641E-4	7.176770311874756E-4	0.0	0.3302903485460017	2.460606964071345E-4
52-53	6.459093280687281E-4	7.228032956959576E-4	0.0	0.410383105226524	2.7681828345802633E-4
54-55	6.56161857085692E-4	7.433083537298855E-4	0.0	0.49150624107325114	3.127021350174001E-4
56-57	6.664143861026559E-4	7.894447343062232E-4	0.0	0.5875519329041694	3.1782839952588203E-4
58-59	6.664143861026559E-4	7.996972633231871E-4	0.0	0.6487646573999525	3.1782839952588203E-4
60-61	7.176770311874756E-4	8.458336438995249E-4	0.0	0.6719866356233758	3.1782839952588203E-4
62-63	7.279295602044395E-4	8.509599084080068E-4	0.0	0.6896517431196048	3.28080928542846E-4
64-65	7.484346182383674E-4	0.0010303791662048757	0.0	0.6981305846166338	3.28080928542846E-4
66-67	7.638134117638133E-4	0.0010713892822727315	0.0	0.7058046025858313	3.28080928542846E-4
68-69	7.689396762722953E-4	0.0011329044563745151	0.0	0.7133248326197744	3.28080928542846E-4
70-71	7.689396762722953E-4	0.0011892933659678167	0.0	0.7190508700757487	3.28080928542846E-4
72-73	7.843184697977412E-4	0.0012354297465441543	0.0	0.7233056696177887	3.588385155937378E-4
74-75	7.996972633231871E-4	0.0012713135981035281	0.0	0.7268889285092177	3.588385155937378E-4
76-77	7.996972633231871E-4	0.001312323714171384	0.0	0.7302261267042395	3.588385155937378E-4
78-79	7.996972633231871E-4	0.001312323714171384	0.0	0.7325483245265818	3.588385155937378E-4
80-81	7.996972633231871E-4	0.001317449978679866	0.0	0.7333326429963796	3.6909104461070174E-4
82-83	7.996972633231871E-4	0.00132770250769683	0.0	0.7340246887050246	3.9984863166159354E-4
84-85	7.996972633231871E-4	0.0013328287722053117	0.0	0.7342758756659402	3.9984863166159354E-4
86-87	8.09949792340151E-4	0.0013430813012222757	0.0	0.734347643369059	3.9984863166159354E-4
88	8.20202321357115E-4	0.0014353540623749511	0.0	0.734347643369059	4.101011606785575E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11240	0.0	48.388123	1
GTATCAA	19520	0.0	33.005085	1
TCAACGC	23115	0.0	27.17966	4
ATCAACG	23090	0.0	27.154526	3
CAACGCA	23675	0.0	26.412596	5
TATCAAC	24615	0.0	25.798073	2
AACGCAG	24525	0.0	25.52841	6
ACGCAGA	28290	0.0	22.023876	7
CGCAGAG	28890	0.0	21.566475	8
GTGGTAT	6245	0.0	19.570229	1
TGGTATC	5815	0.0	18.922865	2
GTACATG	23725	0.0	18.622795	1
TACATGG	23635	0.0	18.320543	2
GCAGAGT	34065	0.0	18.216244	9
ACATGGG	23250	0.0	17.845978	3
GAGTACT	22110	0.0	17.498533	12-13
CAGAGTA	33545	0.0	17.209478	10-11
AGAGTAC	31195	0.0	16.655321	10-11
CATGGGG	17390	0.0	16.131815	4
GTACTTT	25475	0.0	15.229092	14-15
>>END_MODULE