FastQCFastQC Report
Fri 27 May 2016
SRR2138949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15974298
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA192600.048.781981
GTATCAA337600.035.8847471
CTTATAC225850.033.6976661
TCAACGC410550.028.8719354
ATCAACG414650.028.6067543
TATACAC295900.028.2800853
CAACGCA421000.028.1052885
TATCAAC428650.027.8499812
AACGCAG436800.027.0949866
TTATACA297850.026.61182
ACGCAGA510200.023.1939857
CGCAGAG527700.022.408868
TACACAT378750.022.0050245
GTGGTAT99600.021.8500631
ACACATC361050.021.4839556
TGGTATC95050.021.2131372
GTACATG439200.019.916121
CACATCT392800.019.5091787
GCAGAGT608850.019.4912959
ATACACA461900.019.3924624
Produced by FastQC (version 0.11.5)