##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15974298
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42741828154201	34.0	31.0	34.0	31.0	34.0
2	32.559488999140996	34.0	31.0	34.0	31.0	34.0
3	32.60964331578139	34.0	31.0	34.0	31.0	34.0
4	36.13376537735805	37.0	35.0	37.0	35.0	37.0
5	36.041187788033	37.0	35.0	37.0	35.0	37.0
6	36.05619739909697	37.0	35.0	37.0	35.0	37.0
7	36.010107987217964	37.0	35.0	37.0	35.0	37.0
8	36.00479758171533	37.0	35.0	37.0	35.0	37.0
9	37.731010527035366	39.0	38.0	39.0	35.0	39.0
10-11	37.65942418878125	39.0	37.0	39.0	35.0	39.0
12-13	37.63804822596899	39.0	37.0	39.0	35.0	39.0
14-15	39.0566745405651	41.0	38.0	41.0	35.0	41.0
16-17	39.02998466661884	41.0	38.0	41.0	35.0	41.0
18-19	39.03198131147923	41.0	38.5	41.0	35.0	41.0
20-21	39.01924657171766	41.0	39.0	41.0	35.0	41.0
22-23	39.00392967269815	41.0	39.0	41.0	35.0	41.0
24-25	38.95776443315694	41.0	38.0	41.0	35.0	41.0
26-27	38.88956453477286	40.5	38.0	41.0	35.0	41.0
28-29	38.82789497109018	40.0	38.0	41.0	35.0	41.0
30-31	38.75454598271202	40.0	38.0	41.0	34.5	41.0
32-33	38.65901294165398	40.0	38.0	41.0	34.0	41.0
34-35	38.57693831161296	40.0	38.0	41.0	34.0	41.0
36-37	38.54996074053432	40.0	38.0	41.0	34.0	41.0
38-39	38.482038600779084	40.0	38.0	41.0	34.0	41.0
40-41	38.468709487200215	40.0	38.0	41.0	34.0	41.0
42-43	38.41976796305577	40.0	38.0	41.0	33.5	41.0
44-45	38.33331394950643	40.0	38.0	41.0	33.0	41.0
46-47	38.28365714187538	40.0	38.0	41.0	33.0	41.0
48-49	38.23496482695126	40.0	38.0	41.0	33.0	41.0
50-51	38.15616379539475	40.0	37.5	41.0	33.0	41.0
52-53	38.03664195370662	40.0	37.0	41.0	33.0	41.0
54-55	37.909409312701555	40.0	37.0	41.0	33.0	41.0
56-57	37.778353285410134	40.0	36.5	41.0	32.0	41.0
58-59	37.6674573248182	40.0	36.0	41.0	32.0	41.0
60-61	37.507884367793054	39.0	36.0	41.0	32.0	41.0
62-63	37.31285640787685	39.0	35.5	41.0	32.0	41.0
64-65	37.111224039511626	39.0	35.0	41.0	32.0	41.0
66-67	36.87334018795694	38.5	35.0	41.0	31.0	41.0
68-69	36.550219606991476	37.5	35.0	40.0	31.0	41.0
70-71	36.234532207826	37.0	35.0	40.0	31.0	41.0
72-73	35.937294071826656	36.5	35.0	39.0	31.0	41.0
74-75	35.67742575755257	36.0	35.0	39.0	31.0	41.0
76-77	35.392588196721036	36.0	34.5	38.5	31.0	40.5
78-79	35.125231389933646	35.0	34.0	37.0	31.0	39.5
80-81	34.85465325051851	35.0	34.0	37.0	31.0	39.0
82-83	34.567179094023125	35.0	34.0	36.5	30.5	39.0
84-85	34.338212313558756	35.0	34.0	36.0	30.0	37.0
86-87	34.17875829865953	35.0	34.0	36.0	30.0	37.0
88-89	34.04406209588769	35.0	34.0	35.5	31.0	37.0
90-91	33.91515836982606	35.0	34.0	35.0	30.0	36.0
92-93	33.863325254811166	35.0	34.0	35.0	31.0	36.0
94-95	33.81496795971222	35.0	34.0	35.0	31.0	36.0
96-97	33.815466307490894	35.0	34.0	35.0	31.0	36.0
98-99	33.95682136175114	35.0	34.0	35.0	31.0	36.0
100	31.17953103721587	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	31.0
19	91.0
20	230.0
21	621.0
22	2409.0
23	7915.0
24	20373.0
25	41672.0
26	74142.0
27	117698.0
28	168593.0
29	226084.0
30	288680.0
31	358664.0
32	442410.0
33	552540.0
34	720467.0
35	987719.0
36	1450763.0
37	2705913.0
38	4387929.0
39	3128034.0
40	291318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03837915130919	20.729292767669666	14.727451559999693	24.50487652102146
2	14.600159228501164	20.85834516925181	41.68841184751702	22.85308375473001
3	18.14006867790832	25.89973080473496	30.6738307671871	25.28636975016962
4	12.650596602116726	18.448453885109693	37.84511845215358	31.055831060620005
5	13.048337129107516	37.7043242901103	35.75568566319282	13.491652917589366
6	29.894023887371024	37.95143026023747	18.403020014621497	13.751525837770004
7	26.22939048714378	32.6194490674958	22.763479183873997	18.38768126148642
8	24.02849251967129	37.37347957324948	21.043910661989653	17.554117245089582
9	24.854156345399343	17.10728070804739	21.18602645324383	36.852536493309444
10-11	24.053794676190552	26.883443897944915	28.7459173862107	20.316844039653834
12-13	24.792228689418135	24.204212092252718	28.891417673920362	22.112141544408786
14-15	21.937588926060062	25.7483670720648	26.28939246244741	26.02465153942773
16-17	21.743151442250134	29.03886456068459	27.0124730366967	22.205510960368578
18-19	21.796942046803245	27.830305702043972	28.744157200486864	21.628595050665922
20-21	22.327097238961095	26.91919496404594	28.822155022578617	21.931552774414353
22-23	22.028122182059622	27.164229083832414	28.696853662097205	22.11079507201076
24-25	21.950436845124933	27.372365761719024	28.81890430042696	21.858293092729078
26-27	21.82692837599129	27.33928773230413	28.90970597193046	21.924077919774117
28-29	21.505586008808674	27.035976622612	28.987448172066433	22.47098919651289
30-31	21.780956388572097	26.933882770161837	29.07119986169675	22.21396097956931
32-33	21.631553300566175	27.1995443153051	29.032151646426957	22.13675073770177
34-35	21.771742803150325	27.515197225936816	29.079274373458176	21.63378559745469
36-37	21.874189475362066	27.249992599686973	28.63976662664734	22.236051298303618
38-39	21.849583355936282	27.146764273519068	28.904296180236187	22.09935619030846
40-41	22.027046885170442	27.059551633567064	28.881638428223095	22.0317630530394
42-43	22.167776644628383	27.033625066676564	28.749373229777923	22.04922505891713
44-45	21.94177433152648	27.37103478757018	28.547255575219292	22.13993530568404
46-47	22.373346920958035	27.089303580765538	28.008016196892232	22.529333301384195
48-49	22.224668493403467	27.20706067417708	28.04439541087378	22.52387542154567
50-51	21.973913328331935	27.4070614647585	28.18240436782814	22.436620839081428
52-53	22.102598041920572	27.473660506018184	28.301223809809812	22.122517642251434
54-55	22.113253847611954	27.459767369178937	28.024587195087562	22.402391588121542
56-57	22.326331310017263	27.738720458733905	27.640571813435656	22.294376417813176
58-59	21.909692247048508	28.030547350085623	28.084549159114935	21.975211243750937
60-61	22.256551191665014	28.269137200519133	27.516838275222856	21.957473332592997
62-63	21.956121875952277	28.558316562601217	27.510073968595538	21.975487592850968
64-65	21.961036921425645	28.407199623882274	27.52607331213478	22.105690142557307
66-67	22.042310941616726	28.313957785675598	27.432398232949474	22.211333039758202
68-69	22.02222784631421	28.088049775344214	27.586777174565142	22.302945203776435
70-71	22.115260594772206	27.644375224447977	27.7243236259208	22.516040554859018
72-73	22.22348962876254	27.55650519083863	27.690179795955487	22.529825384443342
74-75	22.098163826836263	27.680202734416227	27.723890387189087	22.497743051558423
76-77	22.223774514084138	27.66582874890159	27.602803567038762	22.50759316997551
78-79	22.154641417808783	27.64302158394513	27.599182450597464	22.603154547648618
80-81	22.13684771962642	27.66078566416632	27.660452188746305	22.541914427460956
82-83	22.231180743611322	27.669822326876588	27.700904926603958	22.398092002908133
84-85	22.296218464638148	27.661391365259558	27.658418516873624	22.38397165322867
86-87	22.166058359351947	27.69466070819066	27.742279543219585	22.39700138923781
88-89	22.179901138104544	27.668211757842958	27.719450929402807	22.432436174649688
90-91	22.201974339934882	27.698761533294842	27.711806878590306	22.38745724817997
92-93	22.209816913008908	27.653828860960335	27.795960593687802	22.340393632342955
94-95	22.06534339974789	27.716113959759863	27.84011014940485	22.3784324910874
96-97	22.165597646992566	27.87853244459006	27.8541467855014	22.101723122915978
98-99	23.193000228702154	29.753120025889867	28.09061876031358	18.9632609850944
100	22.299176958194263	30.69030682922913	21.517354227634602	25.493161984942002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4082.0
1	3335.5
2	3631.5
3	4800.0
4	6016.5
5	9773.0
6	13215.0
7	16168.5
8	18356.0
9	17759.5
10	14856.5
11	11562.0
12	11807.5
13	16760.0
14	30655.5
15	50699.0
16	67203.0
17	76903.5
18	80072.0
19	77743.0
20	72400.5
21	66838.5
22	64898.5
23	71134.5
24	85406.0
25	106858.0
26	134972.0
27	167115.5
28	201303.5
29	237995.0
30	273214.5
31	305933.5
32	339669.5
33	373769.0
34	407646.0
35	439653.0
36	476902.0
37	513307.5
38	538336.0
39	559219.0
40	581015.0
41	598480.0
42	610541.0
43	631772.0
44	669015.5
45	712650.5
46	721169.0
47	708023.0
48	690972.5
49	665608.5
50	651560.5
51	634679.0
52	609848.0
53	578642.5
54	542763.0
55	505043.5
56	466143.5
57	423575.0
58	378591.5
59	331363.0
60	279259.0
61	229235.5
62	185339.0
63	146462.0
64	112358.0
65	85219.0
66	65122.0
67	51116.0
68	40326.0
69	30865.0
70	23985.5
71	18826.5
72	14510.0
73	10909.5
74	8142.5
75	6141.5
76	4608.5
77	3429.0
78	2601.5
79	1936.0
80	1390.0
81	962.5
82	713.5
83	537.0
84	391.0
85	270.5
86	189.5
87	145.0
88	100.5
89	67.5
90	49.0
91	38.0
92	27.5
93	16.5
94	10.0
95	7.5
96	7.0
97	6.5
98	4.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006429077509384137
3	1.2520111994905817E-4
4	0.0
5	0.010710955811641925
6	0.012050607795096849
7	0.0
8	0.0
9	0.0
10-11	2.191019599108518E-5
12-13	2.5040223989811634E-5
14-15	9.390083996179363E-6
16-17	2.535322678968428E-4
18-19	9.327483436204835E-4
20-21	0.0
22-23	1.4401285496311973E-4
24-25	0.0
26-27	6.2660950525889855E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.6846560238326045E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.2669459716823473E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.00618465648941477
52-53	5.762004602757065E-5
54-55	4.440808295429896E-5
56-57	0.0
58-59	6.952723738215351E-6
60-61	8.42887640338158E-5
62-63	9.968331568418352E-4
64-65	0.001340392446838387
66-67	0.0010372461303828542
68-69	0.009852820238508024
70-71	8.22170845018266E-4
72-73	5.605483652791979E-4
74-75	4.179164902552513E-4
76-77	7.676162655583824E-5
78-79	0.0
80-81	1.2946676015474493E-4
82-83	0.0
84-85	8.023881639400982E-6
86-87	1.826339097191882E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	580.0
20-21	2086.0
22-23	3900.0
24-25	6616.0
26-27	10351.0
28-29	17031.0
30-31	38944.0
32-33	48038.0
34-35	65516.0
36-37	95646.0
38-39	105579.0
40-41	110704.0
42-43	128388.0
44-45	152725.0
46-47	152851.0
48-49	136714.0
50-51	119257.0
52-53	113240.0
54-55	120834.0
56-57	128466.0
58-59	141257.0
60-61	145492.0
62-63	140840.0
64-65	159465.0
66-67	170988.0
68-69	177505.0
70-71	158119.0
72-73	130247.0
74-75	131558.0
76-77	137827.0
78-79	142774.0
80-81	143956.0
82-83	138982.0
84-85	141702.0
86-87	147615.0
88-89	158581.0
90-91	167342.0
92-93	179480.0
94-95	228562.0
96-97	569562.0
98-99	1275941.0
100-101	9629037.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.1264934999099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.44804694780382	70.94353412290822
2	8.28221486687979	13.438141011255608
3	2.225569557199451	5.416579626472159
4	0.9019308594797736	2.9268195203581597
5	0.42725775828135576	1.7330961874999242
6	0.24383582056594044	1.1868927067312776
7	0.1346717893224903	0.7647815028764571
8	0.09078161836376625	0.5891835497679507
9	0.05906333325454419	0.4312441009222011
>10	0.18258595555754145	2.2535520357929726
>50	0.003156240072863781	0.17388290743101126
>100	8.557882114040319E-4	0.11739736058428707
>500	1.4733622590683404E-5	0.008677784617039053
>1k	1.4731384693496383E-5	0.016217582782772397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.2600559974529085E-6	0.0
2	0.0	1.2520111994905817E-5	0.0	6.2600559974529085E-6	0.0
3	0.0	1.8780167992358725E-5	0.0	2.5040223989811634E-5	0.0
4	0.0	5.008044797962327E-5	0.0	2.5040223989811634E-5	0.0
5	0.0	6.260055997452908E-5	0.0	3.130027998726454E-5	0.0
6	5.008044797962327E-5	6.260055997452908E-5	0.0	3.130027998726454E-5	0.0
7	5.008044797962327E-5	6.260055997452908E-5	0.0	3.130027998726454E-5	0.0
8	5.008044797962327E-5	8.13807279668878E-5	0.0	3.756033598471745E-5	0.0
9	5.008044797962327E-5	1.502413439388698E-4	0.0	5.008044797962327E-5	0.0
10-11	5.008044797962327E-5	1.502413439388698E-4	0.0	6.573058797325554E-5	0.0
12-13	5.008044797962327E-5	1.502413439388698E-4	0.0	7.51206719694349E-5	0.0
14-15	5.008044797962327E-5	1.9719176391976663E-4	0.0	1.0329092395797298E-4	0.0
16-17	9.390083996179362E-5	2.0658184791594599E-4	0.0	1.3772123194396397E-4	0.0
18-19	9.390083996179362E-5	2.191019599108518E-4	0.0	1.7528156792868144E-4	0.0
20-21	9.390083996179362E-5	2.3162207190575762E-4	0.0	2.0971187591467243E-4	0.0
22-23	1.0016089595924654E-4	2.566622958955693E-4	0.0	2.3475209990448406E-4	0.0
24-25	1.095509799554259E-4	2.9735265987901316E-4	0.0	2.597923238942957E-4	0.0
26-27	1.1268100795415235E-4	3.192628558700983E-4	0.0	4.100336678331655E-4	0.0
28-29	1.1894106395160525E-4	3.192628558700983E-4	0.0	0.001192540667514779	0.0
30-31	1.220710919503317E-4	3.630832478522687E-4	0.0	0.003956355390390238	0.0
32-33	1.2833114794778464E-4	3.693433038497216E-4	0.0	0.010075560127900455	0.0
34-35	1.439812879414169E-4	4.100336678331655E-4	0.0	0.018079041720644	0.0
36-37	1.502413439388698E-4	4.601141158127888E-4	0.0	0.030589763631553635	0.0
38-39	1.502413439388698E-4	5.10194563792412E-4	0.0	0.05747357411261515	0.0
40-41	1.502413439388698E-4	5.915752917592999E-4	0.0	0.08406003193379766	0.0
42-43	1.5650139993632272E-4	6.197455437478379E-4	0.0	0.10197631219850788	0.0
44-45	1.5650139993632272E-4	6.792160757236405E-4	0.0	0.12485681686919825	0.0
46-47	1.5650139993632272E-4	7.199064397070845E-4	0.0	0.1478406124638466	0.0
48-49	1.5650139993632272E-4	7.261664957045374E-4	0.0	0.16579758309254028	0.0
50-51	1.5650139993632272E-4	7.825069996816136E-4	0.0	0.18341025064137403	0.0
52-53	1.5650139993632272E-4	7.950271116765194E-4	0.0	0.22867984558695476	0.0
54-55	1.5650139993632272E-4	7.950271116765194E-4	0.0	0.2791578071224163	0.0
56-57	1.5650139993632272E-4	7.950271116765194E-4	0.0	0.335560911659467	0.0
58-59	1.5650139993632272E-4	7.950271116765194E-4	0.0	0.37329026915611563	0.0
60-61	1.5650139993632272E-4	8.20067335666331E-4	0.0	0.38629240546282534	0.0
62-63	1.6902151193122852E-4	8.325874476612368E-4	0.0	0.4004370019890702	0.0
64-65	1.6902151193122852E-4	8.951880076357659E-4	0.0	0.40775500745009263	0.0
66-67	1.7215153992995497E-4	9.202282316255776E-4	0.0	0.4140307135875392	0.0
68-69	1.7528156792868144E-4	9.421384276166627E-4	0.0	0.42039406050895006	0.0
70-71	1.846716519248608E-4	9.609185956090214E-4	0.0	0.42576518855476464	0.0
72-73	2.1284190391339887E-4	9.796987636013802E-4	0.0	0.43010653738899823	0.0
74-75	2.1284190391339887E-4	0.0010016089595924654	0.0	0.43341810701165084	0.0
76-77	2.1284190391339887E-4	0.001039169295577183	0.0	0.43668585624232126	0.0
78-79	2.1284190391339887E-4	0.0010516894075720887	0.0	0.43911788799733176	0.0
80-81	2.1284190391339887E-4	0.0010579494635695416	0.0	0.4398377944370388	0.0
82-83	2.1284190391339887E-4	0.001067339547565721	0.0	0.4405295306247573	0.0
84-85	2.1284190391339887E-4	0.0010861197155580796	0.0	0.4407580226686644	0.0
86-87	2.1284190391339887E-4	0.0011142899675466178	0.0	0.4408456634526287	0.0
88	2.191019599108518E-4	0.0011643704155262409	0.0	0.44088322378861344	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19260	0.0	48.78198	1
GTATCAA	33760	0.0	35.884747	1
CTTATAC	22585	0.0	33.697666	1
TCAACGC	41055	0.0	28.871935	4
ATCAACG	41465	0.0	28.606754	3
TATACAC	29590	0.0	28.280085	3
CAACGCA	42100	0.0	28.105288	5
TATCAAC	42865	0.0	27.849981	2
AACGCAG	43680	0.0	27.094986	6
TTATACA	29785	0.0	26.6118	2
ACGCAGA	51020	0.0	23.193985	7
CGCAGAG	52770	0.0	22.40886	8
TACACAT	37875	0.0	22.005024	5
GTGGTAT	9960	0.0	21.850063	1
ACACATC	36105	0.0	21.483955	6
TGGTATC	9505	0.0	21.213137	2
GTACATG	43920	0.0	19.91612	1
CACATCT	39280	0.0	19.509178	7
GCAGAGT	60885	0.0	19.491295	9
ATACACA	46190	0.0	19.392462	4
>>END_MODULE