FastQCFastQC Report
Fri 27 May 2016
SRR2138948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14022922
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193050.051.846111
GTATCAA351450.036.733761
TCAACGC433700.029.1731954
ATCAACG435500.029.0719493
CAACGCA443900.028.5218185
TATCAAC448800.028.5030752
AACGCAG461450.027.488486
ACGCAGA529850.023.9055677
TGGTATC87100.023.6883452
GTGGTAT95000.023.3584381
CGCAGAG548350.023.1527888
CTTATAC91950.022.3930841
GTACATG465850.020.8073141
TACATGG463550.020.4836222
GCAGAGT623700.020.2949269
GAGTACT356300.019.50609412-13
ACATGGG474700.019.3980673
TATACAC141250.018.8687953
CAGAGTA604150.018.43286310-11
AGAGTAC565900.018.20964810-11
Produced by FastQC (version 0.11.5)