##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138948_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14022922
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41785292680085	34.0	31.0	34.0	31.0	34.0
2	32.54694827511698	34.0	31.0	34.0	31.0	34.0
3	32.595346675963825	34.0	31.0	34.0	31.0	34.0
4	36.12624316101879	37.0	35.0	37.0	35.0	37.0
5	36.03583896423299	37.0	35.0	37.0	35.0	37.0
6	36.05034999125004	37.0	35.0	37.0	35.0	37.0
7	36.00579051926553	37.0	35.0	37.0	35.0	37.0
8	36.00419484612408	37.0	35.0	37.0	35.0	37.0
9	37.724372352638056	39.0	38.0	39.0	35.0	39.0
10-11	37.65426456768425	39.0	37.0	39.0	35.0	39.0
12-13	37.63122036905004	39.0	37.0	39.0	35.0	39.0
14-15	39.04386411048995	41.0	38.0	41.0	35.0	41.0
16-17	39.018299645394876	41.0	38.0	41.0	35.0	41.0
18-19	39.02194200324298	41.0	38.5	41.0	35.0	41.0
20-21	39.00324819341732	41.0	39.0	41.0	35.0	41.0
22-23	38.985203153021175	41.0	39.0	41.0	35.0	41.0
24-25	38.93862216975815	41.0	38.0	41.0	35.0	41.0
26-27	38.875172313172186	40.0	38.0	41.0	35.0	41.0
28-29	38.8157968199924	40.0	38.0	41.0	34.5	41.0
30-31	38.73598173604171	40.0	38.0	41.0	34.0	41.0
32-33	38.63755891530871	40.0	38.0	41.0	34.0	41.0
34-35	38.556214242524675	40.0	38.0	41.0	34.0	41.0
36-37	38.532689746357164	40.0	38.0	41.0	34.0	41.0
38-39	38.47615451412948	40.0	38.0	41.0	34.0	41.0
40-41	38.464898574359395	40.0	38.0	41.0	34.0	41.0
42-43	38.414291569467466	40.0	38.0	41.0	33.5	41.0
44-45	38.322524439577165	40.0	38.0	41.0	33.0	41.0
46-47	38.26504474731426	40.0	38.0	41.0	33.0	41.0
48-49	38.20448693548123	40.0	38.0	41.0	33.0	41.0
50-51	38.14286449480839	40.0	37.0	41.0	33.0	41.0
52-53	38.01346069648518	40.0	37.0	41.0	33.0	41.0
54-55	37.88059203177016	40.0	37.0	41.0	33.0	41.0
56-57	37.75391901832111	40.0	36.5	41.0	32.5	41.0
58-59	37.629266115112586	40.0	36.0	41.0	32.0	41.0
60-61	37.475254099925216	39.0	36.0	41.0	32.0	41.0
62-63	37.25169291409475	39.0	35.0	41.0	32.0	41.0
64-65	37.0285595846113	39.0	35.0	41.0	31.5	41.0
66-67	36.786098224996394	38.0	35.0	40.5	31.0	41.0
68-69	36.452191769883086	37.0	35.0	40.0	31.0	41.0
70-71	36.12675875207442	37.0	35.0	39.5	31.0	41.0
72-73	35.821271041586336	36.0	35.0	39.0	31.0	41.0
74-75	35.55833976536863	36.0	35.0	39.0	31.0	41.0
76-77	35.27784020432236	35.5	34.0	38.5	31.0	40.0
78-79	35.01710455760639	35.0	34.0	37.0	31.0	39.0
80-81	34.75550066102129	35.0	34.0	37.0	31.0	39.0
82-83	34.47630683271376	35.0	34.0	36.0	30.0	39.0
84-85	34.260283368770345	35.0	34.0	36.0	30.0	37.0
86-87	34.11389855546081	35.0	34.0	36.0	30.0	37.0
88-89	33.98754360843	35.0	34.0	35.0	30.0	37.0
90-91	33.86392376572874	35.0	34.0	35.0	30.0	36.0
92-93	33.8182191622608	35.0	34.0	35.0	30.5	36.0
94-95	33.7732041717419	35.0	34.0	35.0	31.0	36.0
96-97	33.776349971522066	35.0	34.0	35.0	31.0	36.0
98-99	33.92369303422737	35.0	34.0	35.0	31.0	36.0
100	31.138140097170396	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	29.0
19	79.0
20	161.0
21	499.0
22	1980.0
23	6521.0
24	16659.0
25	35781.0
26	64133.0
27	101602.0
28	147052.0
29	198031.0
30	254031.0
31	318137.0
32	395143.0
33	496519.0
34	648028.0
35	897991.0
36	1317401.0
37	2443629.0
38	3814077.0
39	2611911.0
40	253527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94076826498785	20.267273824956025	14.658970505576512	24.132987404479607
2	15.056685658520195	20.831377772192894	41.15857557033505	22.95336099895186
3	18.533131121280665	25.703194267264063	30.16068784045643	25.602986770998843
4	13.039386513024889	17.975326397736506	37.627257714191096	31.358029375047515
5	13.402281915945577	37.65940116234661	35.19514333133426	13.743173590373559
6	30.453873811857342	37.41397167471611	18.277865874193562	13.854288639232982
7	26.86291059737764	32.5341251987282	22.571208768044208	18.03175543584996
8	24.556372773092512	37.00287286772329	20.581666217639945	17.85908814154425
9	25.522063090702495	17.08692382372233	21.051133280210788	36.33987980536439
10-11	24.588195552452063	26.727165487240246	28.27923902856738	20.405399931740313
12-13	25.290527075373078	24.170225335047054	28.589184198710132	21.95006339086974
14-15	22.384682022762444	25.441020780119867	26.315884806319257	25.858412390798435
16-17	21.92544231441072	28.625146875960706	27.03691828936509	22.41249252026348
18-19	22.034462338653675	27.64838955682558	28.81820702391094	21.4989410806098
20-21	22.81794818956886	26.70734447337465	28.81329031492911	21.66141702212738
22-23	22.58463822912944	26.67590189817496	28.67093230557235	22.068527567123255
24-25	22.373614175421043	26.837623351381833	28.738042115896306	22.05072035730082
26-27	22.261214294581052	26.956123050561953	28.750323096489655	22.03233955836734
28-29	22.177495919161903	26.90634967692621	28.835759214478852	22.08039518943304
30-31	22.511898623044868	26.823657856496542	28.659245098471263	22.005198421987327
32-33	22.263604851747825	26.934906291635976	28.640909646594686	22.160579210021513
34-35	22.31592553141962	27.047478328783665	28.689341587472317	21.947254552324395
36-37	22.403876259558288	26.897295479640427	28.59688709869614	22.10194116210515
38-39	22.206875004091916	26.991538277437126	28.677121122953224	22.12446559551773
40-41	22.42243023216161	26.90604890916023	28.53332993418285	22.13819092449531
42-43	22.400812712770207	26.954945254252266	28.352173565690563	22.292068467286963
44-45	22.371123717314127	27.127635608593852	28.16479506181997	22.336445612272048
46-47	22.573741070141654	27.020182994591753	27.81763538592907	22.588440549337527
48-49	22.546458793100225	27.155347637378004	27.70880545699289	22.589388112528884
50-51	22.389916871415487	27.306010788878815	27.721711275455963	22.582361064249735
52-53	22.56764258067792	27.23212931252798	27.684573627907294	22.51565447888681
54-55	22.553554936287863	27.170223531526094	27.59514671226031	22.68107481992573
56-57	22.584079981736483	27.331421313773802	27.553611181986692	22.530887522503026
58-59	22.433658372570225	27.411483740895864	27.700102586641506	22.454755299892405
60-61	22.606133760619777	27.47868560541139	27.5005239887739	22.41465664519493
62-63	22.506054360391143	27.67186399605366	27.432160499913195	22.389921143642006
64-65	22.48369238871825	27.543264621750314	27.454524228699313	22.51851876083213
66-67	22.533384179280716	27.535633823242023	27.34882041395928	22.582161583517983
68-69	22.435647597781287	27.46641843137049	27.50096935582389	22.596964615024334
70-71	22.493579628448536	27.23212516769596	27.52896570363698	22.745329500218524
72-73	22.597005374334188	27.26454177605956	27.419983150662215	22.718469698944038
74-75	22.398048579023914	27.404913383224105	27.486707070260973	22.71033096749101
76-77	22.53910704844515	27.351130976115606	27.414448571429446	22.6953134040098
78-79	22.45961737925976	27.330447592435508	27.423429867590155	22.786505160714572
80-81	22.519247307552334	27.37492526550916	27.404591715647143	22.701235711291368
82-83	22.53670849378578	27.337290585341982	27.48077490915869	22.645226011713543
84-85	22.595263650430155	27.36263073009032	27.407165651522586	22.63493996795694
86-87	22.4479538558379	27.410375309076922	27.49766743978597	22.644003395299205
88-89	22.46786758813948	27.373112951382765	27.467505994346237	22.691513466131514
90-91	22.54300060549281	27.40919408793333	27.475913935851125	22.571891370722735
92-93	22.576625678000035	27.358143584376133	27.457725778712373	22.607504958911466
94-95	22.414894929286223	27.440046845746924	27.48272411518981	22.662334109777046
96-97	22.49975308805128	27.571407110773787	27.550993386964663	22.377846414210275
98-99	23.491120766221247	29.4106663990714	27.802429038864478	19.295783795842876
100	22.585562893313753	30.365364149786263	21.16989093701142	25.879182019888564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3814.0
1	3081.0
2	3473.0
3	4686.0
4	5889.0
5	9637.0
6	13206.5
7	16748.0
8	19788.0
9	19553.0
10	16375.5
11	12675.0
12	12634.0
13	17387.5
14	30878.0
15	50071.0
16	65698.5
17	74148.0
18	75475.0
19	71563.5
20	64803.5
21	57510.0
22	53988.5
23	57839.0
24	68667.0
25	86775.5
26	110389.5
27	135178.0
28	160195.5
29	189162.5
30	216698.5
31	243271.5
32	275105.0
33	306300.5
34	336507.5
35	365778.0
36	396892.5
37	425799.0
38	449310.5
39	471065.5
40	490378.0
41	507907.5
42	521394.0
43	538637.0
44	560211.5
45	581965.5
46	592891.5
47	593343.5
48	594943.0
49	590978.5
50	585257.0
51	577802.0
52	562062.0
53	537387.5
54	508319.5
55	477751.5
56	443627.0
57	405860.5
58	366085.0
59	323446.5
60	275700.5
61	228455.5
62	185416.0
63	147505.0
64	115367.5
65	88688.5
66	68087.0
67	53342.0
68	42103.5
69	32561.5
70	25212.5
71	19667.5
72	15317.0
73	11884.5
74	8874.5
75	6603.5
76	4969.0
77	3635.5
78	2737.0
79	2094.5
80	1560.5
81	1139.5
82	866.0
83	630.0
84	437.5
85	304.0
86	218.5
87	167.0
88	112.0
89	74.0
90	56.5
91	47.5
92	39.0
93	23.5
94	11.0
95	7.5
96	8.0
97	7.5
98	3.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006738966386606158
3	1.3549244586827196E-4
4	0.0
5	0.010404393606410989
6	0.01189481051096198
7	0.0
8	0.0
9	0.0
10-11	2.852472544595199E-5
12-13	2.4959134765207992E-5
14-15	0.0
16-17	2.4959134765207994E-4
18-19	9.698406651623678E-4
20-21	0.0
22-23	1.319554803607449E-4
24-25	0.0
26-27	7.138281504412814E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.6771481556835535E-5
36-37	0.0
38-39	3.6372602540822697E-6
40-41	0.0
42-43	1.1070368873547161E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.005909556766358075
52-53	5.030854423674799E-5
54-55	3.122138486901634E-5
56-57	0.0
58-59	0.0
60-61	7.62456757666282E-5
62-63	9.802568175646467E-4
64-65	0.001398432863793825
66-67	9.626482958666898E-4
68-69	0.00938146489861836
70-71	7.561521318948983E-4
72-73	4.995344296420485E-4
74-75	4.0972150970214067E-4
76-77	6.537467709267829E-5
78-79	0.0
80-81	1.3819063612225663E-4
82-83	0.0
84-85	4.562069186698941E-6
86-87	1.9387955481930564E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	496.0
20-21	1788.0
22-23	3626.0
24-25	6066.0
26-27	9222.0
28-29	15476.0
30-31	33916.0
32-33	42285.0
34-35	56767.0
36-37	82716.0
38-39	96084.0
40-41	98146.0
42-43	114913.0
44-45	139435.0
46-47	139345.0
48-49	124532.0
50-51	111395.0
52-53	107020.0
54-55	113661.0
56-57	118880.0
58-59	117753.0
60-61	117017.0
62-63	114376.0
64-65	123341.0
66-67	131053.0
68-69	134392.0
70-71	128862.0
72-73	117613.0
74-75	119729.0
76-77	124390.0
78-79	129242.0
80-81	131437.0
82-83	126441.0
84-85	127316.0
86-87	133410.0
88-89	143005.0
90-91	151487.0
92-93	162323.0
94-95	204056.0
96-97	504167.0
98-99	1120990.0
100-101	8444753.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.00517210212168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59718668409482	73.54024728477094
2	7.699738771062757	12.78236283666886
3	2.0044950148838536	4.991503610648377
4	0.7785528517728125	2.5849565380799975
5	0.3528625836107945	1.4644709740506647
6	0.18886534205624034	0.9406080132902587
7	0.11051866667982457	0.6421534663779102
8	0.07244979723250687	0.48109663104384415
9	0.04255728302580701	0.31792271415802315
>10	0.14854141286468217	1.8968082598842255
>50	0.0031890043196336577	0.1814610366985828
>100	9.95792493447361E-4	0.1384111586054094
>500	3.120099742448446E-5	0.019900955630713674
>1k	1.5594905361485705E-5	0.01809652009218097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.131181361487998E-6
2	0.0	7.131181361487998E-6	0.0	0.0	7.131181361487998E-6
3	0.0	1.4262362722975996E-5	0.0	0.0	7.131181361487998E-6
4	0.0	8.557417633785597E-5	0.0	0.0	7.131181361487998E-6
5	0.0	9.270535769934397E-5	0.0	0.0	7.131181361487998E-6
6	7.131181361487998E-6	9.270535769934397E-5	0.0	7.131181361487998E-6	7.131181361487998E-6
7	7.131181361487998E-6	9.270535769934397E-5	0.0	7.131181361487998E-6	7.131181361487998E-6
8	7.131181361487998E-6	9.983653906083197E-5	0.0	7.131181361487998E-6	1.4262362722975996E-5
9	7.131181361487998E-6	1.9254189676017595E-4	0.0	1.4262362722975996E-5	1.4262362722975996E-5
10-11	1.4262362722975996E-5	1.9254189676017595E-4	0.0	1.4262362722975996E-5	1.4262362722975996E-5
12-13	1.4262362722975996E-5	1.9254189676017595E-4	0.0	1.4262362722975996E-5	1.4262362722975996E-5
14-15	2.1393544084463992E-5	2.888128451402639E-4	0.0	1.4262362722975996E-5	1.7827953403719993E-5
16-17	3.2090316126695994E-5	2.923784358210079E-4	0.0	1.4262362722975996E-5	2.1393544084463992E-5
18-19	3.565590680743999E-5	3.280343426284479E-4	0.0	1.4262362722975996E-5	2.1393544084463992E-5
20-21	3.565590680743999E-5	3.565590680743999E-4	0.0	1.4262362722975996E-5	2.1393544084463992E-5
22-23	3.565590680743999E-5	3.9221497488183986E-4	0.0	1.4262362722975996E-5	3.209031612669599E-5
24-25	3.565590680743999E-5	4.385676537315119E-4	0.0	3.565590680743999E-5	4.2787088168927985E-5
26-27	4.2787088168927985E-5	4.5639560713523186E-4	0.0	7.131181361487997E-5	4.6352678849671984E-5
28-29	4.6352678849671984E-5	4.599611978159759E-4	0.0	1.9967307812166396E-4	4.9918269530415983E-5
30-31	5.704945089190398E-5	5.811912809612718E-4	0.0	7.666019963599598E-4	4.9918269530415983E-5
32-33	5.704945089190398E-5	6.061504157264798E-4	0.0	0.0022748468543146716	6.418063225339199E-5
34-35	6.774622293413598E-5	6.988557734258238E-4	0.0	0.0043642829932306546	6.418063225339199E-5
36-37	7.487740429562399E-5	7.986923124866557E-4	0.0	0.007519830745689094	6.418063225339199E-5
38-39	8.557417633785597E-5	8.307826286133518E-4	0.0	0.012172926584060013	6.418063225339199E-5
40-41	9.270535769934397E-5	9.127912142704637E-4	0.0	0.01708631054212524	6.418063225339199E-5
42-43	9.270535769934397E-5	9.448815303971596E-4	0.0	0.021643135432116073	6.418063225339199E-5
44-45	1.0696772042231997E-4	0.0010946363389884076	0.0	0.026531560255416097	6.418063225339199E-5
46-47	1.2123008314529596E-4	0.0011980384687299837	0.0	0.03239339133455923	8.200858565711198E-5
48-49	1.2123008314529596E-4	0.0012051696500914716	0.0	0.038429936357058825	8.557417633785597E-5
50-51	1.2123008314529596E-4	0.0013192685518752796	0.0	0.044084963176718804	8.557417633785597E-5
52-53	1.2123008314529596E-4	0.0013335309145982557	0.0	0.04966154700140242	9.270535769934397E-5
54-55	1.2836126450678395E-4	0.0013335309145982557	0.0	0.05487800616733089	1.0340212974157596E-4
56-57	1.4618921791050395E-4	0.0013406620959597437	0.0	0.06026561368593507	1.0696772042231997E-4
58-59	1.5688598995273597E-4	0.0013584900493634636	0.0	0.06623797807618127	1.0696772042231997E-4
60-61	1.6401717131422395E-4	0.0013870147748094156	0.0	0.0722531295545964	1.0696772042231997E-4
62-63	1.7827953403719995E-4	0.0013905803654901596	0.0	0.07776909833770737	1.0696772042231997E-4
64-65	1.7827953403719995E-4	0.0015831222622503356	0.0	0.08317809940039601	1.0696772042231997E-4
66-67	1.8184512471794396E-4	0.0015831222622503356	0.0	0.0887939047225678	1.1053331110306397E-4
68-69	1.8541071539868794E-4	0.0016187781690577756	0.0	0.09433483264044398	1.1409890178380796E-4
70-71	1.8541071539868794E-4	0.0016437373038229834	0.0	0.09945502085799236	1.2123008314529596E-4
72-73	1.8897630607943194E-4	0.0016473028945037276	0.0	0.10323098138890026	1.4975480859124796E-4
74-75	1.9254189676017595E-4	0.0017114835267571194	0.0	0.10652202158722697	1.4975480859124796E-4
76-77	1.9254189676017595E-4	0.0017827953403719995	0.0	0.10958842957266682	1.4975480859124796E-4
78-79	1.9254189676017595E-4	0.0018077544751372075	0.0	0.11154950444707601	1.4975480859124796E-4
80-81	2.1750103152538394E-4	0.0018113200658179515	0.0	0.1121734828162062	1.5688598995273594E-4
82-83	2.4246016629059193E-4	0.0018113200658179515	0.0	0.11294365040324691	1.6401717131422395E-4
84-85	2.4959134765207994E-4	0.0018255824285409274	0.0	0.11318967616021824	1.6401717131422395E-4
86-87	2.5672252901356795E-4	0.0018612383353483674	0.0	0.11328594710859834	1.6401717131422395E-4
88	2.5672252901356795E-4	0.0019539436930477113	0.0	0.11330020947132131	1.6401717131422395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19305	0.0	51.84611	1
GTATCAA	35145	0.0	36.73376	1
TCAACGC	43370	0.0	29.173195	4
ATCAACG	43550	0.0	29.071949	3
CAACGCA	44390	0.0	28.521818	5
TATCAAC	44880	0.0	28.503075	2
AACGCAG	46145	0.0	27.48848	6
ACGCAGA	52985	0.0	23.905567	7
TGGTATC	8710	0.0	23.688345	2
GTGGTAT	9500	0.0	23.358438	1
CGCAGAG	54835	0.0	23.152788	8
CTTATAC	9195	0.0	22.393084	1
GTACATG	46585	0.0	20.807314	1
TACATGG	46355	0.0	20.483622	2
GCAGAGT	62370	0.0	20.294926	9
GAGTACT	35630	0.0	19.506094	12-13
ACATGGG	47470	0.0	19.398067	3
TATACAC	14125	0.0	18.868795	3
CAGAGTA	60415	0.0	18.432863	10-11
AGAGTAC	56590	0.0	18.209648	10-11
>>END_MODULE