FastQCFastQC Report
Fri 27 May 2016
SRR2138947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15821920
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224550.050.6238481
GTATCAA402700.035.859971
TCAACGC496300.028.52994
ATCAACG499750.028.383123
TATCAAC514950.027.8805142
CAACGCA509450.027.7852635
AACGCAG527500.026.8309446
GTGGTAT102950.026.5167081
TGGTATC105200.024.3177742
ACGCAGA605850.023.3374217
CGCAGAG641400.022.0504578
GCAGAGT714850.019.8257859
GTACATG524050.019.1830561
GAGTACT421500.018.95211812-13
TACATGG517650.018.8702162
ACATGGG524000.018.1289543
CAGAGTA698050.018.0723410-11
AGAGTAC657900.017.76287310-11
GTACTTT472950.016.87817214-15
AGTACTT452150.016.76945512-13
Produced by FastQC (version 0.11.5)