##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138947_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15821920
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43502033887164	34.0	31.0	34.0	31.0	34.0
2	32.56301378088121	34.0	31.0	34.0	31.0	34.0
3	32.61038761414544	34.0	31.0	34.0	31.0	34.0
4	36.135229289492045	37.0	35.0	37.0	35.0	37.0
5	36.045737116607846	37.0	35.0	37.0	35.0	37.0
6	36.05982308089031	37.0	35.0	37.0	35.0	37.0
7	36.01593592939415	37.0	35.0	37.0	35.0	37.0
8	36.01342523536967	37.0	35.0	37.0	35.0	37.0
9	37.73440511644604	39.0	38.0	39.0	35.0	39.0
10-11	37.66705914958488	39.0	37.0	39.0	35.0	39.0
12-13	37.64545447075955	39.0	37.0	39.0	35.0	39.0
14-15	39.06195584985893	41.0	38.0	41.0	35.0	41.0
16-17	39.038771274282766	41.0	38.0	41.0	35.0	41.0
18-19	39.04442087306724	41.0	38.5	41.0	35.0	41.0
20-21	39.02750202189777	41.0	39.0	41.0	35.0	41.0
22-23	39.01005273430306	41.0	39.0	41.0	35.0	41.0
24-25	38.965422245266815	41.0	38.5	41.0	35.0	41.0
26-27	38.90317107581686	41.0	38.0	41.0	35.0	41.0
28-29	38.84408694964576	40.0	38.0	41.0	35.0	41.0
30-31	38.76142214028155	40.0	38.0	41.0	34.5	41.0
32-33	38.661654194854876	40.0	38.0	41.0	34.0	41.0
34-35	38.58364714951848	40.0	38.0	41.0	34.0	41.0
36-37	38.5621588477433	40.0	38.0	41.0	34.0	41.0
38-39	38.51660970868676	40.0	38.0	41.0	34.0	41.0
40-41	38.49084769253901	40.0	38.0	41.0	34.0	41.0
42-43	38.437883456329104	40.0	38.0	41.0	34.0	41.0
44-45	38.34970571762287	40.0	38.0	41.0	33.0	41.0
46-47	38.30218153715546	40.0	38.0	41.0	33.0	41.0
48-49	38.24478824684843	40.0	38.0	41.0	33.0	41.0
50-51	38.187208566526515	40.0	37.5	41.0	33.0	41.0
52-53	38.05927739986436	40.0	37.0	41.0	33.0	41.0
54-55	37.93199187171794	40.0	37.0	41.0	33.0	41.0
56-57	37.805180365134376	40.0	36.5	41.0	33.0	41.0
58-59	37.68251232572931	40.0	36.0	41.0	32.0	41.0
60-61	37.53599669923773	39.0	36.0	41.0	32.0	41.0
62-63	37.31635196618436	39.0	35.0	41.0	32.0	41.0
64-65	37.09709865038728	39.0	35.0	41.0	32.0	41.0
66-67	36.85640297406312	38.0	35.0	41.0	31.0	41.0
68-69	36.52669067057129	37.5	35.0	40.0	31.0	41.0
70-71	36.20039115523154	37.0	35.0	39.5	31.0	41.0
72-73	35.89581953196975	36.5	35.0	39.0	31.0	41.0
74-75	35.63657774837621	36.0	35.0	39.0	31.0	41.0
76-77	35.34917940474457	35.5	34.0	38.5	31.0	40.0
78-79	35.08566940041714	35.0	34.0	37.0	31.0	39.0
80-81	34.821800778082675	35.0	34.0	37.0	31.0	39.0
82-83	34.539167729067145	35.0	34.0	36.5	31.0	39.0
84-85	34.31878128752413	35.0	34.0	36.0	30.0	37.0
86-87	34.166686089914016	35.0	34.0	36.0	31.0	37.0
88-89	34.034685276008815	35.0	34.0	35.5	31.0	37.0
90-91	33.905560365812164	35.0	34.0	35.0	30.0	36.0
92-93	33.85262559742455	35.0	34.0	35.0	31.0	36.0
94-95	33.80541282814316	35.0	34.0	35.0	31.0	36.0
96-97	33.80931511609762	35.0	34.0	35.0	31.0	36.0
98-99	33.951533227940395	35.0	34.0	35.0	31.0	36.0
100	31.15661984114783	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	20.0
19	90.0
20	176.0
21	523.0
22	2105.0
23	6964.0
24	18126.0
25	38489.0
26	69830.0
27	110894.0
28	160972.0
29	217398.0
30	280539.0
31	352495.0
32	438364.0
33	551212.0
34	719593.0
35	1000129.0
36	1469911.0
37	2715521.0
38	4273061.0
39	3087458.0
40	308045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10883508449038	20.252371393610886	14.662152254593627	23.97664126730511
2	15.051779607986903	20.921938701338103	41.12859571832196	22.897685972353027
3	18.693488495589875	25.557156923550657	30.065044698518616	25.684309882340848
4	12.915303578832404	17.975258375721783	37.77998498285922	31.329453062586587
5	13.353180981908041	37.596106693616214	35.30621463298327	13.744497691492471
6	30.519551283631575	37.45715676008203	18.22993369988474	13.793358256401659
7	26.814160354748353	32.51509930526763	22.656535995631376	18.014204344352645
8	24.479437388129885	37.167638314439714	20.47271127650753	17.880213020922874
9	25.319670431907127	17.100415120288815	21.220149008464205	36.35976543933985
10-11	24.49879957633444	26.904371367459085	28.240511862218437	20.356317193988037
12-13	25.212862198187203	24.410547788006312	28.588490349887806	21.788099663918675
14-15	22.193636700683193	25.68902202628025	26.482573098912532	25.634768174124023
16-17	21.68958429718845	28.831422056038853	27.216076724131394	22.262916922641303
18-19	21.76613406971894	27.832447834735184	29.070171800485443	21.331246295060424
20-21	22.591996383528976	26.86454376817369	29.161033839438698	21.382426008858634
22-23	22.365995464857484	26.810212608156426	28.975713290539257	21.84807863644684
24-25	22.157483055530154	26.908495373066692	29.082466016522112	21.851555554881035
26-27	21.97022335316311	27.035906332813102	29.080121951562848	21.91374836246094
28-29	21.967078513431186	27.089883527112914	29.040999712423567	21.902038247032333
30-31	22.231473940812972	26.866657877851065	29.009435801792044	21.892432379543926
32-33	22.037105317052013	26.963442696299445	28.95035510235146	22.049096884297082
34-35	22.208238626113648	27.049421912054427	28.88511316552401	21.85722629630791
36-37	22.212982975631075	26.973939992017776	28.810129913239745	22.0029471191114
38-39	22.044707177385636	27.130673119260063	28.85097012459114	21.97364957876316
40-41	22.237923198091003	27.019888528059212	28.71481321038038	22.027375063469407
42-43	22.14071189171801	27.129748133603403	28.517061129140842	22.21247884553775
44-45	22.19029844247319	27.199990807964674	28.355260112414353	22.254450637147784
46-47	22.292831598295823	27.249193182021784	28.05386946938479	22.4041057502976
48-49	22.370326425773147	27.40900378519873	27.870809204955886	22.349860584072232
50-51	22.330664036701855	27.46198203500368	27.834953701661107	22.37240022663336
52-53	22.503088424985222	27.401603962019145	27.75252220654786	22.34278540644777
54-55	22.49241883130679	27.373563715600884	27.704704789632405	22.429312663459918
56-57	22.440160604547124	27.522659420988415	27.63235467743704	22.40482529702743
58-59	22.345651233613584	27.479049485501744	27.784678472692715	22.390620808191958
60-61	22.43960823081298	27.464473266363935	27.72548168269136	22.370436820131726
62-63	22.411158028907565	27.526600158479823	27.751456270714957	22.31078554189765
64-65	22.43224280497598	27.428541509610415	27.72491955280624	22.414296132607358
66-67	22.430194134464205	27.475131692176298	27.57635927862543	22.51831489473407
68-69	22.381940416817987	27.48528887045552	27.657996400509067	22.47477431221743
70-71	22.384440261900686	27.326377859483365	27.6839271435079	22.605254735108055
72-73	22.46119536615855	27.387262464694444	27.566293323054893	22.58524884609211
74-75	22.30697115171135	27.47668502416541	27.603985390710463	22.612358433412787
76-77	22.390546279464555	27.47284042405966	27.555613766304187	22.580999530171592
78-79	22.328119439086308	27.426898247630465	27.590512027804625	22.654470285478602
80-81	22.30880279443945	27.47038750176467	27.60744428156267	22.61336542223321
82-83	22.447077000046406	27.429124036410595	27.6633020983984	22.4604968651446
84-85	22.46217179462197	27.457971617281153	27.61334952271084	22.466507065386036
86-87	22.32272196444422	27.506327137006398	27.660825428201846	22.510125470347536
88-89	22.442223108607067	27.407864640553804	27.58792223261842	22.56199001822071
90-91	22.430331622142845	27.49797704139984	27.582557234271977	22.48913410218534
92-93	22.45159579208933	27.45717990383692	27.659935158776104	22.431289145297644
94-95	22.248178063448794	27.59917088206738	27.691009406251528	22.461641648232302
96-97	22.33654595499749	27.699998582236685	27.709553856890075	22.25390160587575
98-99	23.376867953499545	29.54559542913504	27.974056058129992	19.103480559235425
100	22.366921597243188	30.601687405061107	21.3831555888479	25.648235408847803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6399.0
1	4819.5
2	4446.0
3	5748.0
4	6914.0
5	10893.0
6	14997.0
7	19461.5
8	23538.5
9	23843.5
10	20052.5
11	15326.0
12	15108.5
13	20351.5
14	35822.5
15	58271.0
16	76448.5
17	86618.0
18	89389.0
19	86635.5
20	80302.0
21	72753.5
22	68878.5
23	73812.5
24	86948.5
25	107058.5
26	133825.5
27	163820.5
28	195823.5
29	231337.5
30	264974.0
31	297774.0
32	332934.5
33	365683.0
34	397111.0
35	427097.0
36	458753.5
37	489460.0
38	514805.0
39	537001.5
40	556641.0
41	574883.0
42	587873.0
43	601460.5
44	616505.5
45	633495.5
46	650679.0
47	664803.5
48	673517.5
49	669631.5
50	661228.0
51	650963.0
52	626789.0
53	593885.0
54	563056.0
55	530666.5
56	494604.0
57	453846.0
58	411486.5
59	362859.5
60	308745.0
61	260003.5
62	213124.0
63	171156.0
64	135847.5
65	102452.0
66	77290.0
67	59281.0
68	43849.0
69	32213.5
70	24349.5
71	18588.5
72	14162.5
73	10697.5
74	8061.0
75	6040.5
76	4419.0
77	3198.5
78	2424.5
79	1857.0
80	1411.0
81	1039.5
82	795.5
83	550.5
84	356.5
85	251.0
86	190.0
87	154.5
88	104.0
89	69.5
90	58.0
91	46.5
92	35.5
93	20.5
94	9.5
95	7.5
96	6.5
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006446752353696644
3	1.9593070878881958E-4
4	0.0
5	0.0110100417648427
6	0.012880864016503686
7	0.0
8	0.0
9	0.0
10-11	3.792207266880378E-5
12-13	3.476189994640347E-5
14-15	1.264069088960126E-5
16-17	2.5281381779202524E-4
18-19	8.658873259376864E-4
20-21	0.0
22-23	1.1063007049221657E-4
24-25	0.0
26-27	6.326666345061127E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.147119378978443E-5
36-37	0.0
38-39	3.2282766039046655E-6
40-41	0.0
42-43	2.2953877429639235E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.005827609861218754
52-53	8.282959994442133E-5
54-55	5.920879839957921E-5
56-57	0.0
58-59	7.104758888284274E-6
60-61	1.0764849751403737E-4
62-63	8.84139721904721E-4
64-65	0.001156184077384132
66-67	9.280064432485608E-4
68-69	0.009206283088233645
70-71	8.239701223898006E-4
72-73	5.73115539748944E-4
74-75	3.6681399074895115E-4
76-77	7.417310456682243E-5
78-79	0.0
80-81	1.5188538121591922E-4
82-83	0.0
84-85	2.0468696301654543E-5
86-87	2.0718515636035847E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	563.0
20-21	1985.0
22-23	4246.0
24-25	6771.0
26-27	10467.0
28-29	18122.0
30-31	40450.0
32-33	50342.0
34-35	69597.0
36-37	101835.0
38-39	117619.0
40-41	119586.0
42-43	139578.0
44-45	167799.0
46-47	168575.0
48-49	150009.0
50-51	134689.0
52-53	129726.0
54-55	137768.0
56-57	141823.0
58-59	141126.0
60-61	137855.0
62-63	131444.0
64-65	139670.0
66-67	146478.0
68-69	148410.0
70-71	145044.0
72-73	136612.0
74-75	140097.0
76-77	145590.0
78-79	150260.0
80-81	152566.0
82-83	145455.0
84-85	145536.0
86-87	154284.0
88-89	163975.0
90-91	173785.0
92-93	183276.0
94-95	228156.0
96-97	562274.0
98-99	1245593.0
100-101	9392884.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.34442890960759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59575982376046	73.83963006324073
2	7.8710504572527595	13.120164105568739
3	1.9586698725796179	4.897326658578064
4	0.7244986565927661	2.41531707118008
5	0.32868534363149055	1.3697046127962351
6	0.1769066017539808	0.8846507816114947
7	0.10225879867952369	0.5965890823849206
8	0.0622173892064947	0.41483782173296674
9	0.041750642529991396	0.31317151124441767
>10	0.13344094618475702	1.7547211014136668
>50	0.0032556149980784085	0.17817964492607583
>100	0.0014507246367395782	0.16992401425499207
>500	2.758106800254131E-5	0.016315661946742516
>1k	2.7547125290726445E-5	0.029467869120737535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8961036334401894E-5	0.0	0.0	0.0
3	0.0	1.8961036334401894E-5	0.0	0.0	0.0
4	0.0	5.6883109003205676E-5	0.0	6.320345444800631E-6	0.0
5	0.0	6.320345444800631E-5	0.0	6.320345444800631E-6	0.0
6	3.792207266880379E-5	6.320345444800631E-5	0.0	6.320345444800631E-6	6.320345444800631E-6
7	4.4242418113604416E-5	6.320345444800631E-5	0.0	6.320345444800631E-6	6.320345444800631E-6
8	4.4242418113604416E-5	7.584414533760758E-5	0.0	6.320345444800631E-6	1.2640690889601262E-5
9	5.056276355840505E-5	1.1376621800641135E-4	0.0	6.320345444800631E-6	1.8961036334401894E-5
10-11	5.056276355840505E-5	1.1376621800641135E-4	0.0	9.480518167200947E-6	1.8961036334401894E-5
12-13	5.056276355840505E-5	1.1376621800641135E-4	0.0	1.2640690889601262E-5	1.8961036334401894E-5
14-15	5.056276355840505E-5	2.275324360128227E-4	0.0	1.2640690889601262E-5	2.212120905680221E-5
16-17	1.011255271168101E-4	2.3069260873522304E-4	0.0	1.2640690889601262E-5	3.1601727224003155E-5
18-19	1.011255271168101E-4	2.40173126902424E-4	0.0	1.8961036334401894E-5	4.4242418113604416E-5
20-21	1.011255271168101E-4	2.5913416323682586E-4	0.0	2.212120905680221E-5	4.4242418113604416E-5
22-23	1.011255271168101E-4	3.1917744496243183E-4	0.0	3.792207266880379E-5	5.3722936280805366E-5
24-25	1.011255271168101E-4	3.6025969035363595E-4	0.0	6.636362717040662E-5	7.900431806000789E-5
26-27	1.011255271168101E-4	3.855410721328385E-4	0.0	8.532466350480852E-5	8.532466350480851E-5
28-29	1.011255271168101E-4	3.918614175776391E-4	0.0	3.1601727224003155E-4	8.848483622720883E-5
30-31	1.011255271168101E-4	4.929869446944492E-4	0.0	0.001052337516559305	8.848483622720883E-5
32-33	1.011255271168101E-4	4.961471174168495E-4	0.0	0.0026703459504282667	9.164500894960916E-5
34-35	1.011255271168101E-4	6.067531627008606E-4	0.0	0.005072077219452506	9.480518167200947E-5
36-37	1.011255271168101E-4	7.205193807072719E-4	0.0	0.008554587559537655	9.480518167200947E-5
38-39	1.0744587256161073E-4	7.52121107931275E-4	0.0	0.014075409305571007	9.480518167200947E-5
40-41	1.0744587256161073E-4	8.21644907824082E-4	0.0	0.01992804918745639	9.480518167200947E-5
42-43	1.1376621800641135E-4	8.31125425991283E-4	0.0	0.025079130724968902	9.480518167200947E-5
44-45	1.1376621800641135E-4	8.848483622720883E-4	0.0	0.030979173197690293	9.480518167200947E-5
46-47	1.4852811795281482E-4	9.290907803856927E-4	0.0	0.03744488658772134	1.011255271168101E-4
48-49	1.5800863612001578E-4	9.354111258304934E-4	0.0	0.0440717687865948	1.3272725434081324E-4
50-51	1.7696967245441766E-4	0.0010586578620041056	0.0	0.05023410559527541	1.3272725434081324E-4
52-53	1.7696967245441766E-4	0.001061818034726506	0.0	0.05635536015856482	1.4852811795281482E-4
54-55	1.7696967245441766E-4	0.0010681383801713065	0.0	0.06264094370341905	1.643289815648164E-4
56-57	1.9593070878881955E-4	0.0010807790710609078	0.0	0.06933734970218533	1.6748915428721673E-4
58-59	2.1489174512322147E-4	0.0010934197619505092	0.0	0.07569561721965476	1.7064932700961704E-4
60-61	2.275324360128227E-4	0.0011155409710073114	0.0	0.08223085440957861	1.7696967245441766E-4
62-63	2.5281381779202524E-4	0.001150302870953715	0.0	0.0882193817185272	1.7696967245441766E-4
64-65	2.5281381779202524E-4	0.0012577487435153256	0.0	0.09391717313701498	1.7696967245441766E-4
66-67	2.559739905144255E-4	0.0012640690889601262	0.0	0.09959600351916834	1.8012984517681797E-4
68-69	2.622943359592262E-4	0.0012798699525721278	0.0	0.10574569963695936	1.832900178992183E-4
70-71	2.654545086816265E-4	0.00130199116162893	0.0	0.1112791620738823	1.832900178992183E-4
72-73	2.780951995712278E-4	0.0013177920252409317	0.0	0.11564652077623955	2.1489174512322147E-4
74-75	2.780951995712278E-4	0.0013462335797425344	0.0	0.11911639042543509	2.1489174512322147E-4
76-77	2.780951995712278E-4	0.0014062768614681405	0.0	0.12246301333845702	2.1805191784562178E-4
78-79	2.780951995712278E-4	0.0014283980705249427	0.0	0.12471621648952845	2.2121209056802209E-4
80-81	2.844155450160284E-4	0.0014283980705249427	0.0	0.12558526398818853	2.3069260873522304E-4
82-83	2.90735890460829E-4	0.0014378785886921437	0.0	0.126283662159839	2.464934723472246E-4
84-85	2.90735890460829E-4	0.0014536794523041451	0.0	0.12655227684124304	2.464934723472246E-4
86-87	2.9705623590562964E-4	0.001472640488638547	0.0	0.12664708202291503	2.464934723472246E-4
88	3.033765813504303E-4	0.0015548049794209553	0.0	0.12665340236835984	2.464934723472246E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22455	0.0	50.623848	1
GTATCAA	40270	0.0	35.85997	1
TCAACGC	49630	0.0	28.5299	4
ATCAACG	49975	0.0	28.38312	3
TATCAAC	51495	0.0	27.880514	2
CAACGCA	50945	0.0	27.785263	5
AACGCAG	52750	0.0	26.830944	6
GTGGTAT	10295	0.0	26.516708	1
TGGTATC	10520	0.0	24.317774	2
ACGCAGA	60585	0.0	23.337421	7
CGCAGAG	64140	0.0	22.050457	8
GCAGAGT	71485	0.0	19.825785	9
GTACATG	52405	0.0	19.183056	1
GAGTACT	42150	0.0	18.952118	12-13
TACATGG	51765	0.0	18.870216	2
ACATGGG	52400	0.0	18.128954	3
CAGAGTA	69805	0.0	18.07234	10-11
AGAGTAC	65790	0.0	17.762873	10-11
GTACTTT	47295	0.0	16.878172	14-15
AGTACTT	45215	0.0	16.769455	12-13
>>END_MODULE