FastQCFastQC Report
Fri 27 May 2016
SRR2138946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15661721
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210100.049.6336631
GTATCAA377050.035.830531
ATCAACG453200.029.1229523
TCAACGC455300.028.9794164
CAACGCA466950.028.211515
TATCAAC477900.027.9183522
AACGCAG484800.027.2468766
GTGGTAT98350.026.7312811
TGGTATC96500.024.723592
ACGCAGA556550.023.716067
CGCAGAG574150.022.9452658
GCAGAGT665700.019.7960269
GTACATG486850.019.4987981
TACATGG481150.019.2679212
GAGTACT393450.018.8833212-13
ACATGGG489950.018.3811663
CAGAGTA643150.018.15049210-11
AGAGTAC603600.017.66273510-11
GTACTTT443000.016.82426514-15
AGTACTT428350.016.19989212-13
Produced by FastQC (version 0.11.5)