##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15661721
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42823933589418	34.0	31.0	34.0	31.0	34.0
2	32.55652823849946	34.0	31.0	34.0	31.0	34.0
3	32.605072328896675	34.0	31.0	34.0	31.0	34.0
4	36.132170915316394	37.0	35.0	37.0	35.0	37.0
5	36.041895587336796	37.0	35.0	37.0	35.0	37.0
6	36.05798909328036	37.0	35.0	37.0	35.0	37.0
7	36.01575695289171	37.0	35.0	37.0	35.0	37.0
8	36.01291658815784	37.0	35.0	37.0	35.0	37.0
9	37.73238630671559	39.0	38.0	39.0	35.0	39.0
10-11	37.66611143820018	39.0	37.0	39.0	35.0	39.0
12-13	37.646114402114556	39.0	37.0	39.0	35.0	39.0
14-15	39.06240441264406	41.0	38.0	41.0	35.0	41.0
16-17	39.039363234730075	41.0	38.0	41.0	35.0	41.0
18-19	39.04489589617897	41.0	38.5	41.0	35.0	41.0
20-21	39.02988696795575	41.0	39.0	41.0	35.0	41.0
22-23	39.012508321427234	41.0	39.0	41.0	35.0	41.0
24-25	38.970795509896554	41.0	38.5	41.0	35.0	41.0
26-27	38.91031408469735	41.0	38.0	41.0	35.0	41.0
28-29	38.85237029051861	40.0	38.0	41.0	35.0	41.0
30-31	38.77114411123133	40.0	38.0	41.0	34.5	41.0
32-33	38.6753967995627	40.0	38.0	41.0	34.0	41.0
34-35	38.6016833555356	40.0	38.0	41.0	34.0	41.0
36-37	38.57975053346868	40.0	38.0	41.0	34.0	41.0
38-39	38.537747831392444	40.0	38.0	41.0	34.0	41.0
40-41	38.516544129620684	40.0	38.0	41.0	34.0	41.0
42-43	38.46596929905169	40.0	38.0	41.0	34.0	41.0
44-45	38.38232122470008	40.0	38.0	41.0	33.0	41.0
46-47	38.33211835960664	40.0	38.0	41.0	33.0	41.0
48-49	38.281851701839386	40.0	38.0	41.0	33.0	41.0
50-51	38.22413986492002	40.0	38.0	41.0	33.0	41.0
52-53	38.096931347761675	40.0	37.0	41.0	33.0	41.0
54-55	37.98111407710858	40.0	37.0	41.0	33.0	41.0
56-57	37.85829241193967	40.0	37.0	41.0	33.0	41.0
58-59	37.738653708860944	40.0	36.0	41.0	32.5	41.0
60-61	37.59824912338291	39.5	36.0	41.0	32.0	41.0
62-63	37.38274670596206	39.0	35.5	41.0	32.0	41.0
64-65	37.16864525109629	39.0	35.0	41.0	32.0	41.0
66-67	36.93606841167405	38.5	35.0	41.0	31.5	41.0
68-69	36.60860163685585	37.5	35.0	40.0	31.0	41.0
70-71	36.28322162076979	37.0	35.0	40.0	31.0	41.0
72-73	35.977176358722616	36.5	35.0	39.0	31.0	41.0
74-75	35.714115723170494	36.0	35.0	39.0	31.0	41.0
76-77	35.42286788869262	36.0	34.5	38.5	31.0	40.5
78-79	35.152469435653515	35.0	34.0	37.0	31.0	39.5
80-81	34.88059740389944	35.0	34.0	37.0	31.0	39.0
82-83	34.59071730078455	35.0	34.0	36.5	31.0	39.0
84-85	34.36282837974581	35.0	34.0	36.0	31.0	37.0
86-87	34.2021417431459	35.0	34.0	36.0	31.0	37.0
88-89	34.065135424254336	35.0	34.0	35.5	31.0	37.0
90-91	33.93206372589054	35.0	34.0	35.0	31.0	36.0
92-93	33.876431621867596	35.0	34.0	35.0	31.0	36.0
94-95	33.82471400230169	35.0	34.0	35.0	31.0	36.0
96-97	33.823028308113095	35.0	34.0	35.0	31.0	36.0
98-99	33.962313123219246	35.0	34.0	35.0	31.0	36.0
100	31.1872025655445	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	27.0
19	80.0
20	171.0
21	525.0
22	2010.0
23	6749.0
24	17768.0
25	37317.0
26	68002.0
27	107799.0
28	157056.0
29	213687.0
30	275005.0
31	344770.0
32	430779.0
33	540404.0
34	707943.0
35	974283.0
36	1428045.0
37	2641830.0
38	4261865.0
39	3137968.0
40	307631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81040646810143	20.446022502890965	14.72780034837806	24.01577068062954
2	14.859774920876855	20.96461751586693	41.47898100437943	22.69662655887678
3	18.568958667668348	25.69884238516849	30.16677131695783	25.565427630205328
4	12.822511651178054	18.02672899102212	38.111335274073646	31.039424083726175
5	13.23727715760974	37.71451384779429	35.44531888020711	13.602890114388867
6	30.246020278974793	37.70019935163782	18.467964336120986	13.585816033266399
7	26.60623312086839	32.63069237410116	22.87179678401882	17.89127772101163
8	24.16390893440127	37.42662125062757	20.76125605864132	17.648213756329845
9	25.20713400526034	17.253902045630873	21.391729555136372	36.14723439397241
10-11	24.294403537186287	26.986912160444255	28.54242837022819	20.17625593214127
12-13	24.98604000308906	24.445575582061878	28.93214667320448	21.636237741644585
14-15	22.009484142898472	25.80520365546034	26.682671080655822	25.502641120985363
16-17	21.537307717774066	28.946979439433022	27.434999146643218	22.080713696149694
18-19	21.58524938861458	28.008185135568542	29.26691343219236	21.139652043624515
20-21	22.385516604845016	27.106780248526007	29.257264431126035	21.250438715502945
22-23	22.141332200227296	27.05159957082555	29.08992275057893	21.717145478368224
24-25	21.919890426563317	27.15176203333568	29.15819687279272	21.770150667308286
26-27	21.796524029772556	27.280668078351244	29.13776208093716	21.785045810939035
28-29	21.767742463225144	27.369410925300418	29.142326160748695	21.720520450725747
30-31	22.039967585574967	27.149489246068708	29.076097295027502	21.734445873328823
32-33	21.803065635422982	27.290268875251357	29.040517923009922	21.86614756631574
34-35	21.973158359070528	27.35842631413046	28.972862348041446	21.69555297875757
36-37	21.966439530090746	27.23405767932207	28.936626972315693	21.862875818271487
38-39	21.846711647271672	27.39245438790374	28.95801595791009	21.802818006914503
40-41	22.061466299598003	27.275527999461673	28.830157640311928	21.8328480606284
42-43	22.00707843141928	27.341429419190217	28.615956863950746	22.035535285439757
44-45	22.045521135782124	27.490745584921537	28.42304085362773	22.040692425668606
46-47	22.1438074031102	27.496017776032865	28.16416645451227	22.19600836634467
48-49	22.152281968555744	27.60677588211658	28.04823931882565	22.19270283050202
50-51	22.10436297283998	27.724655328177423	27.97369587673438	22.197285822248222
52-53	22.220904421077837	27.68455668722889	27.878979917510982	22.215558974182294
54-55	22.222107115794312	27.594911122190986	27.9099020082201	22.273079753794608
56-57	22.177447135712942	27.683658730441557	27.904035555309708	22.234858578535793
58-59	22.142724653921324	27.712413698577247	27.93710818008895	22.207753467412477
60-61	22.23444005721318	27.661094017235	27.91438771428627	22.19007821126555
62-63	22.205853766082974	27.75900835499865	27.880676000193148	22.154461878725225
64-65	22.16292736315428	27.689557937561727	27.882693838659385	22.26482086062461
66-67	22.205391235240757	27.69977237178996	27.79554777584047	22.299288617128816
68-69	22.19175031499022	27.68373399848178	27.824360820731464	22.300154865796536
70-71	22.158097353354652	27.559750388435177	27.85110580963898	22.43104644857119
72-73	22.218344524458907	27.579328199834702	27.81854515008192	22.38378212562447
74-75	22.103962546402368	27.70247581875278	27.786642692944703	22.40691894190015
76-77	22.1971836370392	27.644506909148376	27.73690341823426	22.421406035578162
78-79	22.154568884068183	27.63513779383842	27.763106962833362	22.447186359260034
80-81	22.17149468820586	27.669481277710517	27.731009871876367	22.42801416220725
82-83	22.19479112678509	27.65208475442289	27.834155389955534	22.318968728836488
84-85	22.297662653007194	27.644140796721988	27.740834002093294	22.31736254817752
86-87	22.10633912941737	27.71560917947037	27.84677023110801	22.33128146000425
88-89	22.180254353337638	27.644473701426904	27.80894273237483	22.36632921286063
90-91	22.190714891125435	27.71142919810049	27.778199044652506	22.31965686612157
92-93	22.209186906703955	27.670245494185675	27.841048323204664	22.27951927590571
94-95	22.09576723405866	27.73600260092471	27.864211900115816	22.30401826490082
96-97	22.136174873383528	27.875456950761603	27.893195879148138	22.095172296706735
98-99	23.165772851393545	29.727260632181206	28.206045234653093	18.900921281772153
100	22.23420453769644	30.68116891238623	21.58237943706053	25.5022471128568
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5115.0
1	3953.0
2	3887.5
3	5009.0
4	6172.0
5	10139.0
6	14059.0
7	17958.0
8	21183.0
9	20983.0
10	17530.0
11	13559.0
12	13735.5
13	19046.0
14	33576.0
15	54101.0
16	71137.0
17	81226.0
18	84223.0
19	81872.0
20	76609.0
21	70759.5
22	69716.5
23	76952.5
24	91256.5
25	112590.5
26	139512.5
27	170070.0
28	204176.0
29	240608.5
30	274680.5
31	309035.0
32	345601.0
33	379267.0
34	411946.5
35	442731.5
36	474197.5
37	505063.5
38	530853.0
39	553355.5
40	574032.0
41	591308.5
42	604210.0
43	619686.0
44	632406.0
45	643137.0
46	651683.0
47	654643.0
48	654919.5
49	646901.5
50	636424.0
51	623682.0
52	601202.0
53	570079.5
54	538341.0
55	504928.0
56	467481.0
57	426657.0
58	382137.0
59	334890.5
60	284166.0
61	236111.5
62	191167.0
63	151258.5
64	117282.5
65	88447.5
66	67012.0
67	51946.0
68	40191.5
69	30425.5
70	23281.5
71	17922.5
72	13534.0
73	10111.0
74	7558.5
75	5693.5
76	4222.5
77	3107.5
78	2338.5
79	1749.5
80	1301.5
81	945.0
82	724.0
83	522.5
84	365.0
85	263.0
86	197.5
87	159.0
88	105.0
89	64.0
90	45.5
91	33.0
92	26.5
93	21.0
94	15.0
95	11.0
96	10.0
97	8.5
98	3.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006423304309915877
3	2.234748020348466E-4
4	0.0
5	0.010969420282739041
6	0.012482663942232146
7	0.0
8	0.0
9	0.0
10-11	4.469496040696932E-5
12-13	3.511746889119019E-5
14-15	0.0
16-17	2.681697624418159E-4
18-19	0.0010215990950164418
20-21	0.0
22-23	1.2772652626732734E-4
24-25	3.19403925838369E-6
26-27	1.5977473551769043E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.8325677955298036E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.9790811897933898E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0059804233222300026
52-53	8.700264070415065E-5
54-55	3.1605483776185276E-5
56-57	0.0
58-59	7.16294639347619E-6
60-61	7.957924283967676E-5
62-63	8.620029046575843E-4
64-65	0.0012317941561324909
66-67	0.0010507870730534748
68-69	0.009243178071294508
70-71	7.388373389044208E-4
72-73	4.5043544980888253E-4
74-75	3.306906096005164E-4
76-77	5.899654429708331E-5
78-79	0.0
80-81	1.1274860564202076E-4
82-83	0.0
84-85	8.247809732267024E-6
86-87	1.2939938066951906E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	534.0
20-21	1948.0
22-23	3780.0
24-25	6340.0
26-27	9865.0
28-29	16941.0
30-31	39537.0
32-33	48135.0
34-35	67264.0
36-37	99490.0
38-39	114538.0
40-41	116615.0
42-43	135194.0
44-45	161413.0
46-47	162886.0
48-49	146179.0
50-51	132131.0
52-53	127494.0
54-55	135671.0
56-57	140166.0
58-59	138507.0
60-61	135433.0
62-63	130685.0
64-65	136949.0
66-67	142972.0
68-69	146142.0
70-71	143379.0
72-73	135823.0
74-75	137758.0
76-77	143848.0
78-79	149327.0
80-81	149960.0
82-83	144573.0
84-85	144438.0
86-87	152771.0
88-89	162441.0
90-91	171153.0
92-93	181002.0
94-95	225350.0
96-97	557841.0
98-99	1234831.0
100-101	9330417.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.59577518817206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.17278693132153	72.82699687094026
2	8.051635903988439	13.300622180456866
3	2.0686300860144167	5.125803165958073
4	0.7876063516111635	2.6021182861780816
5	0.3572172679079318	1.4752318576728278
6	0.18546384585485387	0.9191118070656782
7	0.11166012279881983	0.6455858080122504
8	0.06411373004053528	0.4236418586322564
9	0.04471463786428557	0.3323916158993151
>10	0.15059696787625443	1.929654149588499
>50	0.004219547588217232	0.23501724280008476
>100	0.0013401367732702107	0.1710246651607646
>500	0.0	0.0
>1k	1.447036033182813E-5	0.012800491635085447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.1924971719263805E-5	0.0	0.0	0.0
3	0.0	3.1924971719263805E-5	0.0	0.0	0.0
4	0.0	9.577491515779141E-5	0.0	0.0	0.0
5	0.0	1.0854490384549692E-4	0.0	0.0	0.0
6	5.7464949094674844E-5	1.0854490384549692E-4	0.0	0.0	0.0
7	5.7464949094674844E-5	1.0854490384549692E-4	0.0	0.0	0.0
8	6.384994343852761E-5	1.2769988687705522E-4	0.0	0.0	0.0
9	6.384994343852761E-5	2.0431981900328834E-4	0.0	0.0	0.0
10-11	6.384994343852761E-5	2.0431981900328834E-4	0.0	0.0	0.0
12-13	6.384994343852761E-5	2.0431981900328834E-4	0.0	0.0	0.0
14-15	6.384994343852761E-5	3.384047002241963E-4	0.0	0.0	0.0
16-17	8.938992081393864E-5	3.5755968325575457E-4	0.0	0.0	0.0
18-19	8.938992081393864E-5	3.86292157803092E-4	0.0	0.0	0.0
20-21	9.577491515779141E-5	4.2779462103813493E-4	0.0	6.384994343852761E-6	0.0
22-23	9.577491515779141E-5	4.980295588205153E-4	0.0	6.384994343852761E-6	1.5962485859631902E-5
24-25	9.577491515779141E-5	5.682644966028957E-4	0.0	3.830996606311656E-5	3.1924971719263805E-5
26-27	9.577491515779141E-5	6.065744626660123E-4	0.0	7.342743495430674E-5	3.1924971719263805E-5
28-29	9.577491515779141E-5	6.097669598379386E-4	0.0	2.4901477941025767E-4	3.1924971719263805E-5
30-31	1.0535240667357054E-4	7.151193665115091E-4	0.0	0.0010822565412830429	3.1924971719263805E-5
32-33	1.0854490384549692E-4	7.151193665115091E-4	6.384994343852761E-6	0.003163764697379043	3.830996606311656E-5
34-35	1.0854490384549692E-4	8.204717731850797E-4	6.384994343852761E-6	0.005787997372702527	4.469496040696932E-5
36-37	1.0854490384549692E-4	8.970917053113129E-4	6.384994343852761E-6	0.009481716600621349	4.469496040696932E-5
38-39	1.0854490384549692E-4	9.194391855147975E-4	6.384994343852761E-6	0.01530163894504314	4.7887457578895704E-5
40-41	1.0854490384549692E-4	9.928666204691043E-4	6.384994343852761E-6	0.021424848520797936	5.1079954750822086E-5
42-43	1.0854490384549692E-4	0.0010375615808760736	6.384994343852761E-6	0.027062798526419928	5.1079954750822086E-5
44-45	1.1812239536127607E-4	0.0011365289932057914	6.384994343852761E-6	0.03335521045228682	5.1079954750822086E-5
46-47	1.2769988687705522E-4	0.001200378936644319	6.384994343852761E-6	0.040701146444889424	5.1079954750822086E-5
48-49	1.2769988687705522E-4	0.0012163414225039508	6.384994343852761E-6	0.04761928781645389	5.7464949094674844E-5
50-51	1.5962485859631902E-4	0.001395121264131828	6.384994343852761E-6	0.05426606692840461	5.7464949094674844E-5
52-53	1.7239484728402455E-4	0.0014046987556476072	6.384994343852761E-6	0.06092561602904304	6.065744626660123E-5
54-55	1.7239484728402455E-4	0.0014174687443353129	6.384994343852761E-6	0.06721164296056609	6.384994343852761E-5
56-57	1.7239484728402455E-4	0.001436623727366871	6.384994343852761E-6	0.07383288209514138	6.384994343852761E-5
58-59	1.7239484728402455E-4	0.0014398162245387975	6.384994343852761E-6	0.08092661081116181	6.384994343852761E-5
60-61	1.8516483597173008E-4	0.0014749336934299878	6.384994343852761E-6	0.08799479954980682	6.384994343852761E-5
62-63	2.107048133471411E-4	0.001503666167977325	6.384994343852761E-6	0.09460965369003828	6.384994343852761E-5
64-65	2.107048133471411E-4	0.0016217885633386011	6.384994343852761E-6	0.10085417815832629	6.384994343852761E-5
66-67	2.234748020348466E-4	0.001628173557682454	6.384994343852761E-6	0.10747860979007351	6.384994343852761E-5
68-69	2.234748020348466E-4	0.0016664835237455706	6.384994343852761E-6	0.11363693683471951	6.384994343852761E-5
70-71	2.234748020348466E-4	0.00171117848415254	6.384994343852761E-6	0.11909610699871362	6.384994343852761E-5
72-73	2.4582228223833126E-4	0.0017271409700121718	6.384994343852761E-6	0.12304841849755847	7.661993212623313E-5
74-75	2.4901477941025767E-4	0.0017686434332472147	6.384994343852761E-6	0.12661763033577217	7.661993212623313E-5
76-77	2.5539977375411044E-4	0.0018197233879980366	6.384994343852761E-6	0.13001763982387377	7.661993212623313E-5
78-79	2.5539977375411044E-4	0.0018324933766857422	6.384994343852761E-6	0.13238008773109927	7.661993212623313E-5
80-81	2.5539977375411044E-4	0.0018356858738576686	6.384994343852761E-6	0.13321332949297207	7.661993212623313E-5
82-83	2.5539977375411044E-4	0.0018516483597173007	6.384994343852761E-6	0.13400826128878174	8.300492647008589E-5
84-85	2.5539977375411044E-4	0.0018580333540611533	6.384994343852761E-6	0.13427323855405163	8.300492647008589E-5
86-87	2.5539977375411044E-4	0.001864418348405006	6.384994343852761E-6	0.1343147410172867	8.300492647008589E-5
88	2.8093975112952144E-4	0.0019091133088119754	6.384994343852761E-6	0.13432751100597437	8.300492647008589E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21010	0.0	49.633663	1
GTATCAA	37705	0.0	35.83053	1
ATCAACG	45320	0.0	29.122952	3
TCAACGC	45530	0.0	28.979416	4
CAACGCA	46695	0.0	28.21151	5
TATCAAC	47790	0.0	27.918352	2
AACGCAG	48480	0.0	27.246876	6
GTGGTAT	9835	0.0	26.731281	1
TGGTATC	9650	0.0	24.72359	2
ACGCAGA	55655	0.0	23.71606	7
CGCAGAG	57415	0.0	22.945265	8
GCAGAGT	66570	0.0	19.796026	9
GTACATG	48685	0.0	19.498798	1
TACATGG	48115	0.0	19.267921	2
GAGTACT	39345	0.0	18.88332	12-13
ACATGGG	48995	0.0	18.381166	3
CAGAGTA	64315	0.0	18.150492	10-11
AGAGTAC	60360	0.0	17.662735	10-11
GTACTTT	44300	0.0	16.824265	14-15
AGTACTT	42835	0.0	16.199892	12-13
>>END_MODULE