FastQCFastQC Report
Fri 27 May 2016
SRR2138945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10941226
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153300.052.7511941
GTATCAA288900.038.0478741
ATCAACG361850.029.8486083
TCAACGC366950.029.4112074
CAACGCA370550.029.069635
TATCAAC375950.029.0068382
AACGCAG384800.028.0228186
ACGCAGA436150.024.6734167
CGCAGAG442350.024.3088868
GCAGAGT505050.021.1927289
TACATGG359650.019.8269122
ACATGGG354300.019.5755963
GTGGTAT78500.019.5066151
GAGTACT317350.019.30862212-13
GTACATG388350.019.0010621
CAGAGTA499050.018.59973710-11
TGGTATC77600.018.5062162
AGAGTAC471750.018.42633610-11
CATGGGG254300.018.0792274
GTACTTT356500.017.18937314-15
Produced by FastQC (version 0.11.5)