##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10941226
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.442781823536045	34.0	31.0	34.0	31.0	34.0
2	32.56968423831113	34.0	31.0	34.0	31.0	34.0
3	32.61907431580337	34.0	31.0	34.0	31.0	34.0
4	36.1463134935701	37.0	35.0	37.0	35.0	37.0
5	36.0630301394012	37.0	35.0	37.0	35.0	37.0
6	36.07719729032194	37.0	35.0	37.0	35.0	37.0
7	36.03412039930443	37.0	35.0	37.0	35.0	37.0
8	36.03197457030866	37.0	35.0	37.0	35.0	37.0
9	37.755860175084585	39.0	38.0	39.0	35.0	39.0
10-11	37.6909679500268	39.0	38.0	39.0	35.0	39.0
12-13	37.66761037565625	39.0	37.0	39.0	35.0	39.0
14-15	39.07965894315683	41.0	38.0	41.0	36.0	41.0
16-17	39.05880456175569	41.0	38.0	41.0	35.0	41.0
18-19	39.07169767812127	41.0	38.5	41.0	35.0	41.0
20-21	39.05649670353739	41.0	39.0	41.0	35.0	41.0
22-23	39.03996509352022	41.0	39.0	41.0	35.0	41.0
24-25	38.99474164779016	41.0	39.0	41.0	35.0	41.0
26-27	38.9362821864662	41.0	38.0	41.0	35.0	41.0
28-29	38.88017881593651	40.0	38.0	41.0	35.0	41.0
30-31	38.79136094967754	40.0	38.0	41.0	35.0	41.0
32-33	38.69175144186401	40.0	38.0	41.0	34.0	41.0
34-35	38.61468565309595	40.0	38.0	41.0	34.0	41.0
36-37	38.5982494449225	40.0	38.0	41.0	34.0	41.0
38-39	38.55630979478233	40.0	38.0	41.0	34.0	41.0
40-41	38.54310915915616	40.0	38.0	41.0	34.0	41.0
42-43	38.49825783216241	40.0	38.0	41.0	34.0	41.0
44-45	38.41723753456536	40.0	38.0	41.0	33.5	41.0
46-47	38.376357532763436	40.0	38.0	41.0	33.0	41.0
48-49	38.3326687861658	40.0	38.0	41.0	33.0	41.0
50-51	38.28217906439552	40.0	38.0	41.0	33.0	41.0
52-53	38.158692278117016	40.0	37.0	41.0	33.0	41.0
54-55	38.04762829786345	40.0	37.0	41.0	33.0	41.0
56-57	37.936669964377124	40.0	37.0	41.0	33.0	41.0
58-59	37.8242363803449	40.0	36.5	41.0	33.0	41.0
60-61	37.698063996366066	39.5	36.0	41.0	33.0	41.0
62-63	37.49857180682817	39.0	36.0	41.0	32.0	41.0
64-65	37.29861965820846	39.0	35.0	41.0	32.0	41.0
66-67	37.08055165928536	39.0	35.0	41.0	32.0	41.0
68-69	36.75893963712764	38.0	35.0	40.5	31.5	41.0
70-71	36.4462651451979	37.0	35.0	40.0	31.0	41.0
72-73	36.14286509352193	37.0	35.0	39.0	31.0	41.0
74-75	35.87872743155184	36.0	35.0	39.0	31.0	41.0
76-77	35.585857442147656	36.0	35.0	39.0	31.0	40.5
78-79	35.305114569466866	35.5	34.5	37.5	31.0	39.5
80-81	35.024632774572225	35.0	34.0	37.0	31.0	39.0
82-83	34.723152427373535	35.0	34.0	37.0	31.0	39.0
84-85	34.474401223548824	35.0	34.0	36.0	31.0	38.0
86-87	34.307185945430916	35.0	34.0	36.0	31.0	37.0
88-89	34.15565540212039	35.0	34.0	36.0	31.0	37.0
90-91	34.013752104957014	35.0	34.0	35.0	31.0	36.5
92-93	33.951881911469656	35.0	34.0	35.0	31.0	36.0
94-95	33.8889066189546	35.0	34.0	35.0	31.0	36.0
96-97	33.88087440167696	35.0	34.0	35.0	31.0	36.0
98-99	34.01212662211839	35.0	34.0	35.0	31.0	36.0
100	31.26065282362885	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	18.0
19	61.0
20	111.0
21	318.0
22	1260.0
23	4275.0
24	11186.0
25	24073.0
26	43077.0
27	70236.0
28	103092.0
29	140719.0
30	182529.0
31	231151.0
32	290652.0
33	367991.0
34	484525.0
35	674110.0
36	975356.0
37	1820958.0
38	2943625.0
39	2333765.0
40	238136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.40430204074023	21.06507076994845	14.770483673401866	23.76014351590946
2	14.493735481704412	21.20180730214855	42.24188533875856	22.062571877388486
3	17.87470816750765	26.834952776695225	30.880374452254266	24.409964603542864
4	12.254412805292569	18.334471840724248	39.63288026405816	29.77823508992502
5	12.46099286955277	38.43193780760434	36.1238272038166	12.983242119026283
6	28.87755458349231	38.99712116605664	18.88393127288995	13.241392977561109
7	25.408660784449566	33.366370459763836	23.812843277343873	17.41212547844273
8	23.69498628398682	37.4946738144336	21.41356005259374	17.396779848985844
9	25.20587729382429	17.040594902253183	21.963224230995685	35.79030357292684
10-11	23.85972865463617	27.355667979515086	29.124633924591148	19.659969441257594
12-13	24.67084797939828	24.74441380902552	29.31742261672623	21.267315594849972
14-15	21.80351377473729	26.09579366113027	27.457213429026456	24.643479135105988
16-17	21.062492659629356	29.009775431499545	28.107219451605193	21.820512457265902
18-19	20.9774113482097	28.12549026561274	30.081603504499142	20.815494881678415
20-21	22.082879040324766	27.379078234248976	29.87791867024413	20.66012405518213
22-23	21.627644524688325	27.18007564763287	29.769181017243547	21.42309881043526
24-25	21.309676565411475	27.27617294559497	30.027256353052866	21.38689413594069
26-27	21.3680583646497	27.504751489190294	29.83879861896787	21.28839152719214
28-29	21.186581198013275	27.80107195644274	29.748297434356424	21.26404941118756
30-31	21.791402598799696	27.36769422042681	29.684451117525875	21.15645206324762
32-33	21.408198761329217	27.573924147604345	29.616513022527442	21.401364068538996
34-35	21.446968496158732	27.721848342691775	29.58453931586548	21.24664384528401
36-37	21.550997092967798	27.55479586508589	29.625079787072316	21.269127254873993
38-39	21.387597159968355	27.69440192133602	29.627227484858103	21.29077343383752
40-41	21.726662934519695	27.57418231891833	29.436547249759194	21.262607496802786
42-43	21.624894509243312	27.633552785848703	29.24001185543511	21.501540849472875
44-45	21.667987204796667	28.005227145475708	28.881259255661302	21.445526394066324
46-47	21.72861068823164	27.737230262904195	28.52742050576953	22.00673854309463
48-49	21.81495462798501	27.846784871948117	28.55068157960843	21.787578920458444
50-51	21.550074510241686	28.011394680123935	28.478746030283048	21.95978477935133
52-53	21.740438427699427	27.991773086119352	28.312912629007318	21.954875857173896
54-55	21.829569852518247	27.82901909922823	28.411000406193278	21.930410642060245
56-57	21.879096731031787	28.042591752112923	28.19393125358002	21.884380263275276
58-59	21.752348419798384	28.160180884250067	28.447602803618498	21.639867892333054
60-61	21.85220037235311	28.183454764828852	28.18416991838047	21.780174944437565
62-63	21.77167190216782	28.091112817412668	28.19937743855879	21.93783784186072
64-65	21.622729671146875	28.16484997372485	28.320630990492585	21.891789364635688
66-67	21.845720824898134	28.22430813787205	27.964558518047134	21.965412519182685
68-69	21.80656991026978	28.12452258356799	28.20445209991712	21.86445540624511
70-71	21.78121494483424	27.67697904031399	28.314201655677252	22.227604359174517
72-73	21.92878608710904	27.823155503610845	28.2893390484037	21.958719360876415
74-75	21.577491612765805	28.060840893954904	28.25646843148415	22.10519906179514
76-77	21.61872227447638	28.066777698579166	28.11608546849363	22.198414558450825
78-79	21.812396305882363	28.106523723040844	28.083412265348745	21.99766770572805
80-81	21.716947392623467	27.997945193116042	28.082759241082645	22.202348173177846
82-83	21.837566772571353	27.880508090660488	28.29466667905902	21.98725845770913
84-85	22.19396351505362	27.882684179615826	28.023038457570127	21.900313847760426
86-87	21.58328418527909	28.15252233352193	28.15975840385579	22.104435077343194
88-89	21.88036870194742	27.96614066112181	28.177979887907075	21.9755107490237
90-91	21.920379990484783	27.98721019252858	28.27084898724769	21.82156082973895
92-93	21.94538128341785	28.03157824706873	28.10873583165543	21.914304637857995
94-95	21.659502915007817	28.15264379001887	28.139215643012154	22.048637651961155
96-97	21.636112270265336	28.256908393406878	28.40595591956463	21.701023416763157
98-99	22.59830437758518	30.122223167580792	28.61485624166172	18.664616213172312
100	21.84798749282804	31.37535705568289	21.841137845126145	24.93551760636293
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2848.0
1	2325.5
2	3037.0
3	4362.5
4	5890.5
5	10409.0
6	14220.0
7	16656.5
8	17669.5
9	16205.5
10	13319.0
11	10275.0
12	10792.5
13	15819.0
14	29070.5
15	48099.5
16	63912.0
17	72786.5
18	74622.5
19	71150.0
20	64323.0
21	56685.0
22	53110.5
23	56779.5
24	67120.5
25	85489.0
26	113553.5
27	142655.5
28	162295.0
29	183071.5
30	202724.0
31	219303.5
32	246978.5
33	278779.0
34	311836.0
35	343084.5
36	376158.5
37	403382.5
38	410787.5
39	418942.0
40	435908.5
41	445200.5
42	447618.5
43	455859.0
44	465353.0
45	473108.5
46	474199.5
47	470454.0
48	465153.5
49	454252.5
50	438829.5
51	420620.0
52	402904.5
53	384658.0
54	360971.5
55	328666.0
56	294211.0
57	261832.5
58	231120.5
59	203366.5
60	173075.0
61	137863.5
62	104158.5
63	77329.5
64	57954.0
65	43669.5
66	32800.0
67	25276.5
68	19422.5
69	14425.5
70	11228.5
71	8612.0
72	6491.5
73	4792.0
74	3430.0
75	2556.5
76	1928.5
77	1438.5
78	1025.0
79	664.0
80	473.5
81	352.5
82	269.0
83	199.0
84	152.5
85	113.0
86	86.0
87	63.0
88	35.5
89	20.5
90	18.0
91	15.0
92	12.5
93	10.0
94	6.0
95	4.0
96	4.0
97	3.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006580615371622888
3	1.645153842905722E-4
4	0.0
5	0.011324142285334387
6	0.012612846128943868
7	0.0
8	0.0
9	0.0
10-11	3.198910250094459E-5
12-13	4.5698717858492276E-5
14-15	9.139743571698455E-6
16-17	1.9193461500566756E-4
18-19	8.271467932387102E-4
20-21	0.0
22-23	1.2341290433667005E-4
24-25	0.0
26-27	1.8297386973038114E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.6171562537557676E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.33285213296823E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.006312125788242301
52-53	5.0400103743573545E-5
54-55	3.0540838547587666E-5
56-57	0.0
58-59	2.0804680304099932E-5
60-61	7.887716774176247E-5
62-63	8.983873415628803E-4
64-65	0.001364784707361681
66-67	0.0010598807052631012
68-69	0.009758491634238751
70-71	8.409131537194815E-4
72-73	5.185175875247824E-4
74-75	4.105014246394928E-4
76-77	1.211633411656779E-4
78-79	0.0
80-81	1.3020072750248314E-4
82-83	0.0
84-85	6.072787704693937E-6
86-87	1.6613399186386464E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	411.0
20-21	1384.0
22-23	2526.0
24-25	4797.0
26-27	7470.0
28-29	12979.0
30-31	31890.0
32-33	38779.0
34-35	51996.0
36-37	77878.0
38-39	91650.0
40-41	92732.0
42-43	108630.0
44-45	128526.0
46-47	129023.0
48-49	114111.0
50-51	101724.0
52-53	96375.0
54-55	103012.0
56-57	106122.0
58-59	103968.0
60-61	103939.0
62-63	100518.0
64-65	104060.0
66-67	110042.0
68-69	110431.0
70-71	109434.0
72-73	101974.0
74-75	102463.0
76-77	106670.0
78-79	109199.0
80-81	111601.0
82-83	105167.0
84-85	106710.0
86-87	109688.0
88-89	117801.0
90-91	122956.0
92-93	128977.0
94-95	156484.0
96-97	366931.0
98-99	828705.0
100-101	6321493.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.79795565312419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.01258800938734	68.56414051227269
2	8.064124899990919	12.708731125014777
3	2.38272261409785	5.632610126381355
4	1.0260426111197423	3.2340024067691675
5	0.540297185878655	2.1287156846187023
6	0.3142016502230323	1.485506862024776
7	0.1940265903216361	1.0702229061783823
8	0.12627580522153722	0.7960220239927389
9	0.08002248660212022	0.5675047515473933
>10	0.2535105287410731	2.865111250910836
>50	0.0037195167859727356	0.19720203749324633
>100	0.002082194411750346	0.31290436816500156
>500	2.2700706085314464E-4	0.10689655503823038
>1k	1.362001349927512E-4	0.22915232556070764
>5k	2.2700022498484808E-5	0.10127706403200905
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	9.139743571698455E-6	0.0	0.0	0.0
6	0.0	9.139743571698455E-6	0.0	0.0	0.0
7	0.0	2.7419230715095364E-5	0.0	8.22576921452861E-5	0.0
8	0.0	2.7419230715095364E-5	0.0	9.139743571698455E-5	0.0
9	0.0	7.311794857358764E-5	0.0	1.2795641000377836E-4	0.0
10-11	0.0	7.311794857358764E-5	0.0	1.2795641000377836E-4	0.0
12-13	0.0	7.311794857358764E-5	0.0	1.2795641000377836E-4	0.0
14-15	0.0	8.682756393113532E-5	0.0	1.2795641000377836E-4	0.0
16-17	0.0	9.139743571698455E-5	0.0	1.325262817896276E-4	0.0
18-19	0.0	9.596730750283378E-5	0.0	1.9193461500566754E-4	0.0
20-21	0.0	1.0967692286038146E-4	0.0	2.3763333286415983E-4	0.0
22-23	0.0	1.325262817896276E-4	0.0	2.4220320465000904E-4	9.139743571698455E-6
24-25	0.0	1.7365512786227065E-4	0.0	2.8333205072265206E-4	9.139743571698455E-6
26-27	0.0	1.9193461500566756E-4	0.0	3.7472948643963664E-4	9.139743571698455E-6
28-29	0.0	1.9193461500566756E-4	0.0	9.505333314566394E-4	9.139743571698455E-6
30-31	0.0	2.1021410214906445E-4	0.0	0.0025728378154331153	9.139743571698455E-6
32-33	0.0	2.1021410214906445E-4	0.0	0.005854005757672861	9.139743571698455E-6
34-35	0.0	2.513429482217075E-4	0.0	0.010359899338520199	9.139743571698455E-6
36-37	0.0	2.9247179429435055E-4	0.0	0.016721160864422324	9.139743571698455E-6
38-39	0.0	2.9247179429435055E-4	0.0	0.026061978794698144	9.139743571698455E-6
40-41	0.0	3.1075128143774746E-4	0.0	0.03559473133997963	9.139743571698455E-6
42-43	0.0	3.2446089679529514E-4	0.0	0.04448770183524223	9.139743571698455E-6
44-45	0.0	3.564499992962397E-4	0.0	0.05394733643195013	9.139743571698455E-6
46-47	0.0	3.7472948643963664E-4	0.0	0.06397363513010333	9.139743571698455E-6
48-49	0.0	3.7472948643963664E-4	0.0	0.07382627870039427	9.139743571698455E-6
50-51	0.0	4.387076914415258E-4	0.0	0.08350526714282294	9.139743571698455E-6
52-53	0.0	4.387076914415258E-4	0.0	0.09284151520131291	9.139743571698455E-6
54-55	0.0	4.4327756322737506E-4	0.0	0.1014374440304953	9.139743571698455E-6
56-57	0.0	4.478474350132243E-4	0.0	0.10766160940282195	9.139743571698455E-6
58-59	0.0	4.478474350132243E-4	0.0	0.11439303054337786	9.139743571698455E-6
60-61	0.0	4.5241730679907355E-4	0.0	0.1207177330949932	9.139743571698455E-6
62-63	0.0	4.5698717858492274E-4	0.0	0.12698302731339248	9.139743571698455E-6
64-65	0.0	4.8440640930001814E-4	0.0	0.13302896768607103	9.139743571698455E-6
66-67	0.0	4.8440640930001814E-4	0.0	0.13923942344304013	9.139743571698455E-6
68-69	0.0	5.02685896443415E-4	0.0	0.14554584650751207	9.139743571698455E-6
70-71	0.0	5.255352553726611E-4	0.0	0.1513724330344698	9.139743571698455E-6
72-73	0.0	5.301051271585104E-4	0.0	0.15620278751211245	9.139743571698455E-6
74-75	0.0	5.438147425160581E-4	0.0	0.15989067404329277	9.139743571698455E-6
76-77	0.0	5.758038450170027E-4	0.0	0.1632540996776778	9.139743571698455E-6
78-79	0.0	6.032230757320981E-4	0.0	0.16556188492953167	9.139743571698455E-6
80-81	0.0	6.032230757320981E-4	0.0	0.16651241826098828	9.139743571698455E-6
82-83	0.0	6.032230757320981E-4	0.0	0.1674035432592289	9.139743571698455E-6
84-85	0.0	6.032230757320981E-4	0.0	0.1677828426174544	9.139743571698455E-6
86-87	0.0	6.123628193037964E-4	0.0	0.16787880992495724	9.139743571698455E-6
88	0.0	6.854807678773841E-4	0.0	0.16789708941210063	9.139743571698455E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15330	0.0	52.751194	1
GTATCAA	28890	0.0	38.047874	1
ATCAACG	36185	0.0	29.848608	3
TCAACGC	36695	0.0	29.411207	4
CAACGCA	37055	0.0	29.06963	5
TATCAAC	37595	0.0	29.006838	2
AACGCAG	38480	0.0	28.022818	6
ACGCAGA	43615	0.0	24.673416	7
CGCAGAG	44235	0.0	24.308886	8
GCAGAGT	50505	0.0	21.192728	9
TACATGG	35965	0.0	19.826912	2
ACATGGG	35430	0.0	19.575596	3
GTGGTAT	7850	0.0	19.506615	1
GAGTACT	31735	0.0	19.308622	12-13
GTACATG	38835	0.0	19.001062	1
CAGAGTA	49905	0.0	18.599737	10-11
TGGTATC	7760	0.0	18.506216	2
AGAGTAC	47175	0.0	18.426336	10-11
CATGGGG	25430	0.0	18.079227	4
GTACTTT	35650	0.0	17.189373	14-15
>>END_MODULE