FastQCFastQC Report
Fri 27 May 2016
SRR2138944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18573829
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208650.046.437831
GTATCAA367350.034.4856721
TCAACGC429350.028.7156874
ATCAACG432750.028.4996533
CAACGCA439400.028.049395
TATCAAC458800.027.1656042
AACGCAG456700.027.0476976
ACGCAGA532400.023.1749787
CGCAGAG538550.022.8715528
GCAGAGT641550.019.1670029
GTGGTAT117900.019.1013681
GTACATG469800.018.5608961
TACATGG459850.018.344662
TGGTATC115800.018.0045192
GAGTACT394250.017.97368812-13
ACATGGG462350.017.7291723
AGAGTAC576650.017.24624410-11
CAGAGTA633400.017.17817910-11
TATACCG13850.016.5910685
CATGGGG349700.016.2123374
Produced by FastQC (version 0.11.5)