##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18573829
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42313790010665	34.0	31.0	34.0	31.0	34.0
2	32.55437212219408	34.0	31.0	34.0	31.0	34.0
3	32.605392296871045	34.0	31.0	34.0	31.0	34.0
4	36.130429972193674	37.0	35.0	37.0	35.0	37.0
5	36.03947785887336	37.0	35.0	37.0	35.0	37.0
6	36.054000281794345	37.0	35.0	37.0	35.0	37.0
7	36.011229510081094	37.0	35.0	37.0	35.0	37.0
8	36.00783704856979	37.0	35.0	37.0	35.0	37.0
9	37.728678346290366	39.0	38.0	39.0	35.0	39.0
10-11	37.6641731761394	39.0	37.0	39.0	35.0	39.0
12-13	37.64263830575806	39.0	37.0	39.0	35.0	39.0
14-15	39.0615068384661	41.0	38.0	41.0	35.0	41.0
16-17	39.03626675469016	41.0	38.0	41.0	35.0	41.0
18-19	39.04029182674181	41.0	38.5	41.0	35.0	41.0
20-21	39.02423694614997	41.0	39.0	41.0	35.0	41.0
22-23	39.00727003971024	41.0	39.0	41.0	35.0	41.0
24-25	38.966709285745814	41.0	38.5	41.0	35.0	41.0
26-27	38.907001996617055	41.0	38.0	41.0	35.0	41.0
28-29	38.85116432772623	40.0	38.0	41.0	35.0	41.0
30-31	38.776969162065804	40.0	38.0	41.0	34.5	41.0
32-33	38.69275267144999	40.0	38.0	41.0	34.0	41.0
34-35	38.620762318938574	40.0	38.0	41.0	34.0	41.0
36-37	38.60163032119745	40.0	38.0	41.0	34.0	41.0
38-39	38.55907911625306	40.0	38.0	41.0	34.0	41.0
40-41	38.54235236287819	40.0	38.0	41.0	34.0	41.0
42-43	38.49408364119734	40.0	38.0	41.0	34.0	41.0
44-45	38.412806500328074	40.0	38.0	41.0	33.5	41.0
46-47	38.37104434920167	40.0	38.0	41.0	33.0	41.0
48-49	38.32018930787788	40.0	38.0	41.0	33.0	41.0
50-51	38.26172988277381	40.0	38.0	41.0	33.0	41.0
52-53	38.13444956732907	40.0	37.0	41.0	33.0	41.0
54-55	38.01457194080638	40.0	37.0	41.0	33.0	41.0
56-57	37.893315075466354	40.0	37.0	41.0	33.0	41.0
58-59	37.77477164828566	40.0	36.0	41.0	33.0	41.0
60-61	37.63635447582885	39.5	36.0	41.0	32.0	41.0
62-63	37.424720652830295	39.0	35.5	41.0	32.0	41.0
64-65	37.21327175880535	39.0	35.0	41.0	32.0	41.0
66-67	36.98496958453525	38.5	35.0	41.0	32.0	41.0
68-69	36.66281181547135	37.5	35.0	40.0	31.0	41.0
70-71	36.340868551535124	37.0	35.0	40.0	31.0	41.0
72-73	36.03722468797913	37.0	35.0	39.0	31.0	41.0
74-75	35.7713782514864	36.0	35.0	39.0	31.0	41.0
76-77	35.482208308860365	36.0	34.5	39.0	31.0	40.5
78-79	35.20771958556427	35.0	34.0	37.5	31.0	39.5
80-81	34.93020326320648	35.0	34.0	37.0	31.0	39.0
82-83	34.63543748757307	35.0	34.0	36.5	31.0	39.0
84-85	34.40244155373679	35.0	34.0	36.0	31.0	37.5
86-87	34.23580321399568	35.0	34.0	36.0	31.0	37.0
88-89	34.09203253068561	35.0	34.0	36.0	31.0	37.0
90-91	33.955170148248996	35.0	34.0	35.0	31.0	36.0
92-93	33.89562328203	35.0	34.0	35.0	31.0	36.0
94-95	33.842003675387815	35.0	34.0	35.0	31.0	36.0
96-97	33.837106742530636	35.0	34.0	35.0	31.0	36.0
98-99	33.973665341996266	35.0	34.0	35.0	31.0	36.0
100	31.185371723228386	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	29.0
19	103.0
20	221.0
21	586.0
22	2434.0
23	7967.0
24	20978.0
25	44652.0
26	79576.0
27	126270.0
28	184468.0
29	248759.0
30	323107.0
31	403788.0
32	502530.0
33	633858.0
34	828822.0
35	1137349.0
36	1673839.0
37	3094926.0
38	5023803.0
39	3844225.0
40	391538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16752819249063	20.817420037623908	14.56575270505613	24.449299064829336
2	14.703744476669964	21.013954871756656	41.821874847557545	22.460425804015834
3	18.61258431837235	25.816479439666594	30.014015980252612	25.556920261708445
4	12.815133594693911	18.091810794640136	38.42890445475729	30.66415115590867
5	13.249224265725179	37.619075285222046	35.6350628930733	13.496637555979484
6	29.953303904127885	38.021473031849645	18.686375209769512	13.338847854252956
7	26.26484824426886	32.42004112345387	23.4255467733659	17.889563858911377
8	23.57931689798587	38.02138482054508	21.142285739790108	17.25701254167894
9	24.931272921700742	17.369859494237833	21.84474725163024	35.85412033243119
10-11	24.26703896126061	27.158089141752544	28.739993005747362	19.83487889123948
12-13	24.717793042569127	24.432081825689696	29.19082712340692	21.659298008334254
14-15	21.833956360530525	25.901472760488573	26.79013717581535	25.47443370316555
16-17	21.531731416079168	28.936999109228413	27.550822301431833	21.980447173260593
18-19	21.545350032887956	28.17034426531452	29.18006130075862	21.104244401038912
20-21	22.25428262381422	27.34372398322909	29.108629034609322	21.293364358347368
22-23	21.99351304929225	27.303610100993563	28.918899168848334	21.78397768086585
24-25	21.831476369069847	27.360785584827596	29.04556943036293	21.762168615739625
26-27	21.8021282370449	27.536147887769285	28.896283155205143	21.765440719980667
28-29	21.72707790817628	27.61794779014895	28.921576293369128	21.733398008305645
30-31	22.004217679253596	27.415992505911763	28.882405722642428	21.697384092192213
32-33	21.77472422087802	27.54995712924377	28.846021802841744	21.82929684703647
34-35	21.897499900086856	27.60527225156736	28.8252368729411	21.671990975404682
36-37	21.916678720696897	27.50485614968774	28.757609806208873	21.82085532340649
38-39	21.806639091347048	27.644066800591677	28.809845762397586	21.73944834566369
40-41	22.080888827198038	27.5362155353183	28.622110634214227	21.760785003269444
42-43	21.94071321501818	27.591188363459807	28.514031244526667	21.954067176995352
44-45	22.03233885315374	27.72124761207459	28.33551125709466	21.910902277677014
46-47	22.065075191337936	27.713475000020676	28.043254539370967	22.17819526927042
48-49	22.057865997673193	27.79700923516527	28.020771185045607	22.12435358211593
50-51	22.00126390807204	27.879691961587778	27.982443481420628	22.136600648919558
52-53	22.105609503296897	27.87766857546109	27.853592772554524	22.16312914868749
54-55	22.11440721293384	27.785921196801116	27.916940786666643	22.182730803598393
56-57	22.107467113035884	27.83658432444236	27.911549191875253	22.144399370646507
58-59	22.083389015708203	27.94358544040439	27.94623941059079	22.02678613329662
60-61	22.19266524070602	27.911967188250014	27.860687347647684	22.034680223396283
62-63	22.150312615109435	27.95931085229949	27.81837081320265	22.07200571938842
64-65	22.102758276973034	27.915939517205985	27.87347222834614	22.10782997747484
66-67	22.115770882820293	27.899466483700348	27.780198713705996	22.20456391977337
68-69	22.0722490110638	27.858737831245346	27.8633990907445	22.205614066946357
70-71	22.056466187877103	27.696825281666026	27.895545518429177	22.351163012027694
72-73	22.142648444458228	27.689515224507343	27.86434139933306	22.303494931701366
74-75	21.957139826658263	27.842364039721225	27.8955016207144	22.304994512906113
76-77	22.088008704840764	27.815082561762228	27.76991196228437	22.326996771112633
78-79	22.002812831634238	27.784476203879322	27.79409515052552	22.418615813960912
80-81	22.022328554761618	27.846229102681185	27.838468997730047	22.292973344827146
82-83	22.04909033803614	27.807492917592096	27.920016620549916	22.22340012382185
84-85	22.200761682790564	27.805090340566696	27.821786335220043	22.172361641422693
86-87	21.99331305609959	27.858475483297195	27.91542588323156	22.23278557737165
88-89	22.072710292698165	27.784981406638842	27.865851088092647	22.276457212570342
90-91	22.11469756794627	27.86138730407197	27.867448337730444	22.156466790251315
92-93	22.11043267924998	27.814424285029293	27.91362378394207	22.161519251778657
94-95	22.005854921510906	27.89581730204108	27.91653219544151	22.181795581006504
96-97	22.015828061212357	28.034246372711802	27.972657352000823	21.97726821407502
98-99	23.064822598963122	29.881446280440766	28.24140843344598	18.812322687150132
100	22.146884627226125	30.801554886030285	21.623563119856087	25.427997366887507
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3336.0
1	2807.5
2	3667.5
3	5199.5
4	7070.0
5	12496.5
6	17051.0
7	19987.0
8	21516.0
9	20121.0
10	16792.5
11	13109.5
12	13395.0
13	18813.5
14	33294.0
15	54154.0
16	71753.0
17	82303.0
18	85524.5
19	83639.5
20	79386.0
21	75225.5
22	75999.5
23	86892.0
24	107705.5
25	136009.5
26	172123.0
27	213023.0
28	254397.5
29	300258.0
30	345177.0
31	386702.0
32	429951.0
33	471053.0
34	512309.5
35	551035.5
36	589217.0
37	623805.0
38	649607.0
39	670528.0
40	694563.5
41	717778.5
42	734624.0
43	752827.0
44	770216.5
45	786166.5
46	794858.0
47	795278.0
48	791780.5
49	780695.0
50	763591.0
51	740571.0
52	710337.5
53	675194.0
54	636490.0
55	589742.5
56	540175.0
57	489435.5
58	434174.5
59	377846.0
60	317248.0
61	257686.0
62	205297.5
63	161383.0
64	124171.0
65	94747.0
66	72353.0
67	56210.0
68	44032.5
69	33072.5
70	25223.0
71	19349.5
72	14568.5
73	10715.0
74	7911.0
75	5850.0
76	4302.0
77	3259.0
78	2446.5
79	1772.0
80	1317.0
81	942.0
82	703.5
83	511.5
84	369.5
85	291.5
86	243.0
87	194.5
88	119.5
89	68.5
90	44.5
91	28.0
92	19.0
93	10.0
94	4.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006713747606915085
3	1.6151758476940861E-4
4	0.0
5	0.011144713349089194
6	0.012140738455167213
7	0.0
8	0.0
9	0.0
10-11	4.0379396192352154E-5
12-13	2.4227637715411294E-5
14-15	5.38391949231362E-6
16-17	2.3689245766179927E-4
18-19	8.614271187701794E-4
20-21	0.0
22-23	1.4539640255912747E-4
24-25	2.6932366310561535E-6
26-27	1.077776530850208E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.075313532888215E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.2354398814769776E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0057936929724310225
52-53	7.644973094987379E-5
54-55	5.342647269255739E-5
56-57	0.0
58-59	9.088938168438383E-6
60-61	7.96032476165563E-5
62-63	8.815827980862662E-4
64-65	0.0012436917408150162
66-67	0.0010423462975101443
68-69	0.009209851595843983
70-71	8.660730210077968E-4
72-73	5.65459966382261E-4
74-75	4.2961974323478617E-4
76-77	9.692535073524396E-5
78-79	0.0
80-81	1.541059949937469E-4
82-83	0.0
84-85	7.02102333100095E-6
86-87	1.848573859039773E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	616.0
20-21	2358.0
22-23	4355.0
24-25	7349.0
26-27	11090.0
28-29	18923.0
30-31	48303.0
32-33	59346.0
34-35	80364.0
36-37	122710.0
38-39	143418.0
40-41	143660.0
42-43	160852.0
44-45	191080.0
46-47	193574.0
48-49	178853.0
50-51	163280.0
52-53	157220.0
54-55	166919.0
56-57	173090.0
58-59	172322.0
60-61	169297.0
62-63	162903.0
64-65	168143.0
66-67	177408.0
68-69	179214.0
70-71	177862.0
72-73	168036.0
74-75	167958.0
76-77	174711.0
78-79	181973.0
80-81	183989.0
82-83	176127.0
84-85	176312.0
86-87	184682.0
88-89	196073.0
90-91	208044.0
92-93	217559.0
94-95	267864.0
96-97	650888.0
98-99	1448997.0
100-101	1.0936107E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.80008206184894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.92373254012729	68.4959725728424
2	8.080738293707263	12.735256813289148
3	2.3445867943610934	5.542608953903446
4	1.0895401885404856	3.434234250666905
5	0.5451528093276304	2.147904305563238
6	0.3133514965911202	1.4815274187350072
7	0.19997289694924655	1.1030516482822403
8	0.12980263877650347	0.8182766869946406
9	0.08896031233605628	0.6309071921096052
>10	0.2798834552682902	3.2905675060146957
>50	0.003407372164449434	0.17715990297327655
>100	8.141186562557743E-4	0.10305587800916102
>500	4.28188984784241E-5	0.024032730249923584
>1k	1.4264295845005724E-5	0.015444140366356541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.076783898462724E-5	2.153567796925448E-5	0.0	0.0	0.0
3	1.076783898462724E-5	2.153567796925448E-5	0.0	0.0	0.0
4	1.076783898462724E-5	3.2303516953881726E-5	0.0	0.0	0.0
5	1.076783898462724E-5	3.2303516953881726E-5	0.0	0.0	0.0
6	1.1306230933858603E-4	3.2303516953881726E-5	0.0	0.0	0.0
7	1.1306230933858603E-4	3.2303516953881726E-5	0.0	0.0	0.0
8	1.1306230933858603E-4	3.2303516953881726E-5	0.0	0.0	0.0
9	1.1306230933858603E-4	6.999095340007707E-5	0.0	0.0	5.38391949231362E-6
10-11	1.1575426908474284E-4	6.999095340007707E-5	0.0	0.0	5.38391949231362E-6
12-13	1.1844622883089965E-4	6.999095340007707E-5	0.0	0.0	1.0767838984627242E-5
14-15	1.2113818857705645E-4	9.691055086164516E-5	0.0	0.0	4.845527543082258E-5
16-17	1.9382110172329033E-4	1.076783898462724E-4	0.0	0.0	5.38391949231362E-5
18-19	1.9920502121560397E-4	1.2652210806937008E-4	0.0	2.69195974615681E-6	5.9223114415449824E-5
20-21	1.9920502121560397E-4	1.4536582629246775E-4	0.0	5.38391949231362E-6	5.9223114415449824E-5
22-23	2.0458894070791758E-4	1.5882562502325182E-4	0.0	1.6151758476940863E-5	8.075879238470431E-5
24-25	2.099728602002312E-4	1.7228542375403587E-4	0.0	3.768743644619534E-5	9.691055086164516E-5
26-27	2.099728602002312E-4	1.7766934324634948E-4	0.0	6.460703390776345E-5	9.691055086164516E-5
28-29	2.099728602002312E-4	1.7766934324634948E-4	0.0	2.3958441740795612E-4	1.0229447035395879E-4
30-31	2.099728602002312E-4	1.8574522248481992E-4	0.0	0.0010148688243011175	1.0229447035395879E-4
32-33	2.099728602002312E-4	1.884371822309767E-4	0.0	0.0028777049686416305	1.1306230933858603E-4
34-35	2.2074069918485842E-4	2.2343265893101525E-4	0.0	0.0053543079351058955	1.1844622883089965E-4
36-37	2.2074069918485842E-4	2.584281356310538E-4	0.0	0.009184966653887038	1.1844622883089965E-4
38-39	2.2074069918485842E-4	2.6919597461568103E-4	0.0	0.015147657491624372	1.292140678155269E-4
40-41	2.3150853816948568E-4	2.745798941079946E-4	0.0	0.021110348329361706	1.3998190680015413E-4
42-43	2.961155720772491E-4	2.7727185385415144E-4	0.0	0.027229172832376136	1.4805778603862457E-4
44-45	3.0688341106187636E-4	3.1495929030034677E-4	0.0	0.033813706371475696	1.56133665277095E-4
46-47	3.176512500465036E-4	3.4995476700038533E-4	0.0	0.041009314772952846	1.642095445155654E-4
48-49	3.2303516953881723E-4	3.4995476700038533E-4	0.0	0.04815915985874533	1.7766934324634948E-4
50-51	3.3380300852344446E-4	3.714904449696398E-4	0.0	0.05517171499748383	1.7766934324634948E-4
52-53	3.391869280157581E-4	3.714904449696398E-4	0.0	0.061357838494152175	1.8574522248481992E-4
54-55	3.391869280157581E-4	3.768743644619534E-4	0.0	0.06782392580442084	1.9651306146944715E-4
56-57	3.553386864926989E-4	3.768743644619534E-4	0.0	0.07503030204488262	1.9920502121560397E-4
58-59	3.661065254773262E-4	3.768743644619534E-4	0.0	0.08215861145270584	1.9920502121560397E-4
60-61	3.849502437004239E-4	3.82258283954267E-4	0.0	0.08912809523550583	2.0458894070791758E-4
62-63	3.8764220344658065E-4	3.957180826850511E-4	0.0	0.09557803078729754	2.2074069918485842E-4
64-65	3.903341631927375E-4	4.576331568466577E-4	0.0	0.1022379391992895	2.2074069918485842E-4
66-67	4.1456180090814875E-4	4.6301707633897136E-4	0.0	0.10895168680620458	2.2343265893101525E-4
68-69	4.2532963989277603E-4	4.657090360851282E-4	0.0	0.11586194747458911	2.288165784233289E-4
70-71	4.307135593850896E-4	4.68400995831285E-4	0.0	0.12185424986953416	2.6112009537721057E-4
72-73	4.4148139836971685E-4	4.710929555774418E-4	0.0	0.12638751008206223	2.7996381360030826E-4
74-75	4.576331568466578E-4	4.81860794562069E-4	0.0	0.1303554587478974	2.7996381360030826E-4
76-77	4.6301707633897136E-4	4.926286335466963E-4	0.0	0.13406767123784763	2.7996381360030826E-4
78-79	4.710929555774418E-4	5.007045127851667E-4	0.0	0.13664118475517353	2.7996381360030826E-4
80-81	4.7916883481591223E-4	5.007045127851667E-4	0.0	0.13738685760485897	2.8803969283877867E-4
82-83	4.845527543082258E-4	5.033964725313235E-4	0.0	0.13822405708591373	3.014994915695627E-4
84-85	4.845527543082258E-4	5.168562712621076E-4	0.0	0.13853363245672176	3.014994915695627E-4
86-87	5.033964725313235E-4	5.330080297390484E-4	0.0	0.13861977516859877	3.014994915695627E-4
88	5.060884322774803E-4	5.599276272006165E-4	0.0	0.1386251590880911	3.0688341106187636E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20865	0.0	46.43783	1
GTATCAA	36735	0.0	34.485672	1
TCAACGC	42935	0.0	28.715687	4
ATCAACG	43275	0.0	28.499653	3
CAACGCA	43940	0.0	28.04939	5
TATCAAC	45880	0.0	27.165604	2
AACGCAG	45670	0.0	27.047697	6
ACGCAGA	53240	0.0	23.174978	7
CGCAGAG	53855	0.0	22.871552	8
GCAGAGT	64155	0.0	19.167002	9
GTGGTAT	11790	0.0	19.101368	1
GTACATG	46980	0.0	18.560896	1
TACATGG	45985	0.0	18.34466	2
TGGTATC	11580	0.0	18.004519	2
GAGTACT	39425	0.0	17.973688	12-13
ACATGGG	46235	0.0	17.729172	3
AGAGTAC	57665	0.0	17.246244	10-11
CAGAGTA	63340	0.0	17.178179	10-11
TATACCG	1385	0.0	16.591068	5
CATGGGG	34970	0.0	16.212337	4
>>END_MODULE