FastQCFastQC Report
Fri 27 May 2016
SRR2138943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17120888
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222550.046.3043751
GTATCAA384600.032.2317771
TCAACGC426950.028.264674
ATCAACG431050.028.0250283
CAACGCA439100.027.492165
TATCAAC456550.026.6268652
AACGCAG455400.026.5242216
ACGCAGA528200.022.850017
CGCAGAG545450.022.1119468
GTGGTAT109250.021.954031
GTACATG456100.020.1858981
TACATGG460700.019.4545962
GAGTACT381550.019.16279812-13
GCAGAGT633150.019.0757169
TGGTATC116650.018.7937642
ACATGGG467650.018.7057063
CAGAGTA626300.017.70589410-11
GTACTTT421500.017.37260614-15
AGAGTAC577200.016.92352310-11
CATGGGG384550.016.6971954
Produced by FastQC (version 0.11.5)