##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17120888
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40891868459159	34.0	31.0	34.0	31.0	34.0
2	32.53841664053874	34.0	31.0	34.0	31.0	34.0
3	32.58834074494267	34.0	31.0	34.0	31.0	34.0
4	36.11718597773667	37.0	35.0	37.0	35.0	37.0
5	36.024441606066226	37.0	35.0	37.0	35.0	37.0
6	36.04204887036233	37.0	35.0	37.0	35.0	37.0
7	35.99983598981548	37.0	35.0	37.0	35.0	37.0
8	35.997002083069525	37.0	35.0	37.0	35.0	37.0
9	37.71454775009334	39.0	38.0	39.0	35.0	39.0
10-11	37.64444063882668	39.0	37.0	39.0	35.0	39.0
12-13	37.622702075967084	39.0	37.0	39.0	35.0	39.0
14-15	39.035641755264095	41.0	38.0	41.0	35.0	41.0
16-17	39.00895304028623	41.0	38.0	41.0	35.0	41.0
18-19	39.011240918111255	41.0	38.5	41.0	35.0	41.0
20-21	38.99360787611783	41.0	39.0	41.0	35.0	41.0
22-23	38.97683275467158	40.5	38.5	41.0	35.0	41.0
24-25	38.934879860467674	41.0	38.0	41.0	35.0	41.0
26-27	38.87075831970935	40.0	38.0	41.0	35.0	41.0
28-29	38.81160069021397	40.0	38.0	41.0	34.0	41.0
30-31	38.73578071075604	40.0	38.0	41.0	34.0	41.0
32-33	38.64661936041438	40.0	38.0	41.0	34.0	41.0
34-35	38.5708516742628	40.0	38.0	41.0	34.0	41.0
36-37	38.5480680615148	40.0	38.0	41.0	34.0	41.0
38-39	38.49793900140171	40.0	38.0	41.0	34.0	41.0
40-41	38.47885594194614	40.0	38.0	41.0	34.0	41.0
42-43	38.42616843227855	40.0	38.0	41.0	33.5	41.0
44-45	38.338175304226695	40.0	38.0	41.0	33.0	41.0
46-47	38.28589956377125	40.0	38.0	41.0	33.0	41.0
48-49	38.2273760448859	40.0	38.0	41.0	33.0	41.0
50-51	38.168158301354666	40.0	37.5	41.0	33.0	41.0
52-53	38.040691289903876	40.0	37.0	41.0	33.0	41.0
54-55	37.91101106379696	40.0	37.0	41.0	33.0	41.0
56-57	37.783043249901	40.0	36.5	41.0	32.5	41.0
58-59	37.657845529080625	40.0	36.0	41.0	32.0	41.0
60-61	37.51152552724437	39.0	36.0	41.0	32.0	41.0
62-63	37.28965884271415	39.0	35.0	41.0	32.0	41.0
64-65	37.06546166800457	39.0	35.0	41.0	31.5	41.0
66-67	36.8271593785966	38.0	35.0	40.5	31.0	41.0
68-69	36.49357460360986	37.0	35.0	40.0	31.0	41.0
70-71	36.16512892193391	37.0	35.0	39.5	31.0	41.0
72-73	35.86001670186921	36.5	35.0	39.0	31.0	41.0
74-75	35.597382822807624	36.0	35.0	39.0	31.0	41.0
76-77	35.31238726942736	35.5	34.0	38.5	31.0	40.0
78-79	35.04663155285818	35.0	34.0	37.0	31.0	39.0
80-81	34.78319621461668	35.0	34.0	37.0	31.0	39.0
82-83	34.50046288533906	35.0	34.0	36.5	30.0	39.0
84-85	34.28250896787105	35.0	34.0	36.0	30.0	37.0
86-87	34.130428338362435	35.0	34.0	36.0	30.0	37.0
88-89	33.99764718857549	35.0	34.0	35.5	30.0	37.0
90-91	33.87132306863903	35.0	34.0	35.0	30.0	36.0
92-93	33.82408445661196	35.0	34.0	35.0	30.5	36.0
94-95	33.78018142995195	35.0	34.0	35.0	31.0	36.0
96-97	33.778714277479054	35.0	34.0	35.0	31.0	36.0
98-99	33.924178929157975	35.0	34.0	35.0	31.0	36.0
100	31.13779154235139	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	22.0
19	82.0
20	199.0
21	616.0
22	2405.0
23	7728.0
24	20570.0
25	43643.0
26	77862.0
27	123883.0
28	178864.0
29	241195.0
30	310702.0
31	387850.0
32	481923.0
33	604837.0
34	787565.0
35	1082008.0
36	1587697.0
37	2941401.0
38	4655089.0
39	3272521.0
40	312223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.83369390653102	20.324150242674328	14.460663488949871	24.381492361844785
2	15.058609302160397	20.851635719279095	41.1501519175916	22.939603060968906
3	19.01390327679878	25.19181868009403	29.870942200553525	25.92333584255367
4	13.258401082934485	17.700869253977945	37.592209002243344	31.448520660844224
5	13.588044398608448	37.39229587233604	35.25229416078797	13.767365568267548
6	30.840660780174844	37.26423291744687	18.152691871039274	13.74241443133901
7	26.94305342106087	32.26062807022627	22.619475111337685	18.176843397375183
8	24.23957799385172	37.57513044884121	20.513550465373058	17.671741091934017
9	25.247049101658746	17.23903573225875	21.21346743229674	36.300447733785774
10-11	24.709235911714007	26.74325793330209	28.27866660954344	20.26883954544046
12-13	25.211147872914598	24.116922608039932	28.735569411865235	21.936360107180235
14-15	22.273938127508337	25.527738397681244	26.305863924815114	25.892459549995305
16-17	21.93806866523	28.688519730783202	27.051804704655847	22.321606899330952
18-19	21.987018614347136	27.819083579041575	28.817299456965817	21.376598349645466
20-21	22.670372767518604	26.927374109146037	28.809043311315364	21.59320981202
22-23	22.529508100279433	26.766798216232058	28.65127159159746	22.05242209189105
24-25	22.335540700304453	26.97615093474304	28.588143003813116	22.10016536113939
26-27	22.274797462855766	26.98564452034492	28.659437087931845	22.08012092886747
28-29	22.201135805572104	27.092747626103403	28.636416663770635	22.069699904553858
30-31	22.373277747899436	26.97647871431735	28.53865320149368	22.11159033628953
32-33	22.19069432499612	27.070543846242533	28.571669376902914	22.167092451858434
34-35	22.352352660522296	27.064311146025027	28.534885820844185	22.048450372608492
36-37	22.373449943725802	27.022371221233282	28.448529841367314	22.155648993673598
38-39	22.284307106709385	27.147341456569446	28.520173921230153	22.048177515491012
40-41	22.456271925934963	27.046106230052924	28.34811756015016	22.14950428386196
42-43	22.326926814096705	27.229310827107312	28.14280305755067	22.300959301245314
44-45	22.444530977243975	27.21253433160348	28.052628226334818	22.290306464817725
46-47	22.445319698774323	27.298841193806293	27.779079181436927	22.47675992598245
48-49	22.388308061318067	27.496896057655313	27.652779894688017	22.462015986338603
50-51	22.346251702005457	27.480369166194336	27.698176189618028	22.475202942182182
52-53	22.530163216980178	27.421566501921866	27.527139505679337	22.521130775418616
54-55	22.50947016960562	27.34411332977562	27.59838418430486	22.548032316313904
56-57	22.480403765704093	27.394909147000025	27.61456369983647	22.51012338745941
58-59	22.44588944354562	27.528908044421385	27.61381976877478	22.411382743258216
60-61	22.53291498723482	27.431480283772242	27.625805543615968	22.409799185376972
62-63	22.558046357971424	27.541347618169564	27.482855739967633	22.417750283891376
64-65	22.508792516737458	27.48530531682185	27.504357335611076	22.501544830829616
66-67	22.572881925876153	27.461325998588578	27.391405387340562	22.574386688194707
68-69	22.351991491089784	27.480430767004872	27.54113676650999	22.62644097539535
70-71	22.395207242464252	27.34680271363228	27.547644543265555	22.710345500637917
72-73	22.50457777821782	27.37696623004522	27.41266903423547	22.705786957501488
74-75	22.31295329798897	27.501157236239916	27.51407982540649	22.671809640364625
76-77	22.50325835499526	27.43691567140405	27.43385747467047	22.62596849893022
78-79	22.370681472581836	27.44428433271165	27.42873684994775	22.756297344758764
80-81	22.491597167397032	27.43835333071123	27.41566767521863	22.65438182667311
82-83	22.372009716786838	27.517370286256458	27.550870196707557	22.559749800249154
84-85	22.518993780587536	27.528631946316683	27.41510263220649	22.537271640889294
86-87	22.3870736574588	27.519297092559018	27.508334233966565	22.585295016015618
88-89	22.401058081768056	27.476077074852835	27.49774071757211	22.625124125807
90-91	22.424530392360992	27.52646930329267	27.478099002859363	22.570901301486973
92-93	22.538178217013737	27.435339155420245	27.518731511145965	22.507751116420046
94-95	22.288059463656918	27.600463831672624	27.582041510880646	22.529435193789816
96-97	22.348972157994133	27.6696540326812	27.643863290497748	22.337510518826914
98-99	23.432998446750084	29.544016064224614	27.782044405845248	19.240941083180054
100	22.516258819226316	30.535234563443254	21.15223987226092	25.796266745069513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3979.0
1	3195.5
2	3559.0
3	4876.5
4	6113.0
5	9730.5
6	13240.5
7	16750.5
8	19649.5
9	19981.5
10	17754.5
11	14423.0
12	14122.0
13	18102.5
14	30583.5
15	49245.0
16	64964.5
17	74011.0
18	76703.5
19	74708.5
20	70085.0
21	65047.5
22	64170.0
23	71003.0
24	85822.0
25	108962.5
26	138276.5
27	168171.5
28	199083.5
29	233784.5
30	267432.0
31	303775.0
32	345303.0
33	385321.5
34	421471.0
35	453906.5
36	494319.5
37	536856.5
38	567134.5
39	585468.5
40	604951.5
41	629716.5
42	650122.0
43	671664.5
44	693870.0
45	707428.5
46	712360.5
47	715139.5
48	719210.5
49	721936.0
50	715601.5
51	703197.5
52	683653.0
53	654290.0
54	624081.0
55	590548.5
56	552832.0
57	508836.0
58	459551.5
59	404484.5
60	340754.5
61	278913.5
62	223173.0
63	175198.0
64	135339.0
65	102713.0
66	77444.0
67	59939.5
68	46684.5
69	35622.5
70	27367.0
71	21017.0
72	15943.5
73	11718.5
74	8624.0
75	6493.5
76	4876.0
77	3557.0
78	2714.0
79	2133.5
80	1598.0
81	1141.0
82	847.0
83	614.0
84	434.5
85	312.5
86	238.0
87	185.0
88	125.5
89	84.5
90	57.5
91	40.5
92	29.5
93	17.5
94	6.5
95	3.0
96	2.0
97	1.5
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006308084019941022
3	1.869061931834377E-4
4	0.0
5	0.010998261305137912
6	0.011874384085685277
7	0.0
8	0.0
9	0.0
10-11	3.796532049038578E-5
12-13	4.3806139027368204E-5
14-15	0.0
16-17	2.307123322108059E-4
18-19	8.323166415199959E-4
20-21	0.0
22-23	1.2268311477239068E-4
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	7.365108769399328E-5
36-37	0.0
38-39	2.9793275484922057E-6
40-41	0.0
42-43	2.1165876977875612E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.006105279029171349
52-53	7.928752106709078E-5
54-55	2.8794426320485996E-5
56-57	0.0
58-59	0.0
60-61	8.232300504521475E-5
62-63	0.0010205059874781588
64-65	0.001415963030346101
66-67	0.0010201099942921286
68-69	0.009500786743230998
70-71	8.377859753866097E-4
72-73	4.93294518168457E-4
74-75	3.8173804767568893E-4
76-77	6.430783388386198E-5
78-79	0.0
80-81	1.2064878775936886E-4
82-83	0.0
84-85	7.4847907181409695E-6
86-87	1.704068379947114E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	551.0
20-21	2184.0
22-23	4401.0
24-25	7182.0
26-27	10528.0
28-29	16972.0
30-31	41383.0
32-33	50268.0
34-35	69962.0
36-37	104572.0
38-39	122127.0
40-41	122470.0
42-43	138646.0
44-45	166953.0
46-47	168777.0
48-49	154037.0
50-51	141067.0
52-53	135114.0
54-55	144596.0
56-57	149961.0
58-59	148571.0
60-61	144209.0
62-63	139030.0
64-65	145646.0
66-67	153734.0
68-69	156257.0
70-71	153750.0
72-73	145448.0
74-75	148870.0
76-77	155228.0
78-79	161231.0
80-81	163154.0
82-83	155248.0
84-85	154806.0
86-87	165041.0
88-89	175737.0
90-91	185813.0
92-93	197350.0
94-95	247816.0
96-97	620510.0
98-99	1366347.0
100-101	1.0285341E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.48647784484099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34524756987727	72.87288306369673
2	7.848346204601519	12.9476487064901
3	2.0024172653572343	4.9551704218504975
4	0.7955834509704435	2.624995068087825
5	0.369971197199383	1.52588104805081
6	0.20163612875934425	0.9979352440576292
7	0.12983822158040945	0.7496928311464198
8	0.07823590422992877	0.5162723344746495
9	0.05132082691088108	0.380994682676698
>10	0.1739496432312369	2.1477759035278203
>50	0.002513316818199785	0.13128309637906432
>100	8.613491543549414E-4	0.09968966512640594
>500	6.5780405191383E-5	0.03830977583402601
>1k	1.3140904619307178E-5	0.011468158601306658
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.840818536982427E-6
2	0.0	5.840818536982427E-6	0.0	0.0	5.840818536982427E-6
3	0.0	5.840818536982427E-6	0.0	0.0	5.840818536982427E-6
4	0.0	2.336327414792971E-5	0.0	5.840818536982427E-6	5.840818536982427E-6
5	0.0	2.336327414792971E-5	0.0	1.752245561094728E-5	5.840818536982427E-6
6	8.177145951775398E-5	2.336327414792971E-5	0.0	1.752245561094728E-5	1.1681637073964855E-5
7	8.177145951775398E-5	2.336327414792971E-5	0.0	1.752245561094728E-5	1.1681637073964855E-5
8	8.177145951775398E-5	2.336327414792971E-5	0.0	2.336327414792971E-5	1.752245561094728E-5
9	8.177145951775398E-5	9.929391512870126E-5	0.0	2.9204092684912137E-5	2.336327414792971E-5
10-11	9.345309659171884E-5	9.929391512870126E-5	0.0	3.212450195340335E-5	2.336327414792971E-5
12-13	1.0513473366568369E-4	9.929391512870126E-5	0.0	3.504491122189456E-5	2.336327414792971E-5
14-15	1.1097555220266612E-4	1.3433882635059582E-4	0.0	4.088572975887699E-5	4.672654829585942E-5
16-17	1.3433882635059582E-4	1.4017964488757825E-4	0.0	4.3806139027368204E-5	5.2567366832841847E-5
18-19	1.3433882635059582E-4	1.4310005415606945E-4	0.0	4.672654829585942E-5	5.8408185369824275E-5
20-21	1.3433882635059582E-4	1.4602046342456068E-4	0.0	4.964695756435063E-5	5.8408185369824275E-5
22-23	1.4017964488757825E-4	1.6354291903550796E-4	0.0	7.301023171228034E-5	9.929391512870128E-5
24-25	1.4310005415606945E-4	1.8106537464645525E-4	0.0	9.929391512870126E-5	1.3433882635059582E-4
26-27	1.4602046342456068E-4	1.8690619318343767E-4	0.0	1.7522455610947282E-4	1.4602046342456068E-4
28-29	1.4602046342456068E-4	1.8982660245192887E-4	0.0	5.665593980872954E-4	1.489408726930519E-4
30-31	1.4602046342456068E-4	2.1026946733136739E-4	0.0	0.002061808943554797	1.547816912300343E-4
32-33	1.518612819615431E-4	2.1026946733136739E-4	0.0	0.005928430815037164	1.6354291903550796E-4
34-35	1.6354291903550796E-4	2.365531507477883E-4	0.0	0.011220212409543243	1.693837375724904E-4
36-37	1.693837375724904E-4	2.6575724343270047E-4	0.0	0.018322647750513878	1.7522455610947282E-4
38-39	1.7230414684098162E-4	2.9204092684912135E-4	0.0	0.028745588429759016	1.8106537464645525E-4
40-41	1.8690619318343767E-4	3.329266566079984E-4	0.0	0.0384559492474923	1.927470117204201E-4
42-43	1.927470117204201E-4	3.41687884413472E-4	0.0	0.0471324851841797	2.0442864879438496E-4
44-45	1.9858783025740253E-4	3.854940234408402E-4	0.0	0.05649823770823102	2.2195110440533224E-4
46-47	2.0442864879438496E-4	4.2053893466273477E-4	0.0	0.0660100106957069	2.482347878217532E-4
48-49	2.0442864879438496E-4	4.23459343931226E-4	0.0	0.07484132832362433	2.5699601562722684E-4
50-51	2.1903069513684104E-4	4.614246644216118E-4	0.0	0.0831615743295558	2.6283683416420924E-4
52-53	2.2195110440533224E-4	4.614246644216118E-4	0.0	0.09120438145498061	2.803592897751565E-4
54-55	2.2195110440533224E-4	4.6434507369010297E-4	0.0	0.09859009649499488	2.9204092684912135E-4
56-57	2.2195110440533224E-4	4.7310630149557665E-4	0.0	0.10476384168858531	2.9204092684912135E-4
58-59	2.2195110440533224E-4	4.7894712003255905E-4	0.0	0.11082953173924157	2.949613361176126E-4
60-61	2.365531507477883E-4	4.877083478380327E-4	0.0	0.11676672378208419	2.978817453861038E-4
62-63	2.745184712381741E-4	5.023103941804888E-4	0.0	0.12278860769371308	3.037225639230862E-4
64-65	2.745184712381741E-4	5.665593980872954E-4	0.0	0.128293579164819	3.037225639230862E-4
66-67	2.803592897751565E-4	5.665593980872954E-4	0.0	0.13407014869789463	3.037225639230862E-4
68-69	2.8620010831213895E-4	5.724002166242779E-4	0.0	0.1397970712734059	3.037225639230862E-4
70-71	3.037225639230862E-4	5.811614444297515E-4	0.0	0.14498079772497782	3.1540420099705107E-4
72-73	3.387674751449808E-4	5.840818536982427E-4	0.0	0.14889122573548758	3.387674751449808E-4
74-75	3.41687884413472E-4	5.87002262966734E-4	0.0	0.1520335861083841	3.387674751449808E-4
76-77	3.475287029504544E-4	6.0160430930919E-4	0.0	0.15516718525347517	3.387674751449808E-4
78-79	3.562899307559281E-4	6.074451278461724E-4	0.0	0.15733996974923264	3.387674751449808E-4
80-81	3.562899307559281E-4	6.103655371146636E-4	0.0	0.15806715165708696	3.475287029504544E-4
82-83	3.592103400244193E-4	6.162063556516462E-4	0.0	0.15882937847616316	3.562899307559281E-4
84-85	3.650511585614017E-4	6.220471741886285E-4	0.0	0.15904256835276304	3.592103400244193E-4
86-87	3.6797156782989295E-4	6.42490039068067E-4	0.0	0.15912433981228075	3.621307492929105E-4
88	3.6797156782989295E-4	6.716941317529792E-4	0.0	0.15913310104008624	3.621307492929105E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22255	0.0	46.304375	1
GTATCAA	38460	0.0	32.231777	1
TCAACGC	42695	0.0	28.26467	4
ATCAACG	43105	0.0	28.025028	3
CAACGCA	43910	0.0	27.49216	5
TATCAAC	45655	0.0	26.626865	2
AACGCAG	45540	0.0	26.524221	6
ACGCAGA	52820	0.0	22.85001	7
CGCAGAG	54545	0.0	22.111946	8
GTGGTAT	10925	0.0	21.95403	1
GTACATG	45610	0.0	20.185898	1
TACATGG	46070	0.0	19.454596	2
GAGTACT	38155	0.0	19.162798	12-13
GCAGAGT	63315	0.0	19.075716	9
TGGTATC	11665	0.0	18.793764	2
ACATGGG	46765	0.0	18.705706	3
CAGAGTA	62630	0.0	17.705894	10-11
GTACTTT	42150	0.0	17.372606	14-15
AGAGTAC	57720	0.0	16.923523	10-11
CATGGGG	38455	0.0	16.697195	4
>>END_MODULE