FastQCFastQC Report
Fri 27 May 2016
SRR2138942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15873562
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190800.047.2304461
GTATCAA339550.035.0663641
ATCAACG400500.028.9887083
TCAACGC401250.028.9344334
CAACGCA411250.028.1485235
TATCAAC424800.027.7202642
AACGCAG430750.026.9296366
ACGCAGA495950.023.334837
CGCAGAG501400.023.0474328
GTACATG430550.019.878441
GTGGTAT100800.019.8201161
TACATGG425350.019.5343322
GCAGAGT592400.019.5071229
ACATGGG425750.018.9385133
TGGTATC96400.018.5293482
GAGTACT360850.018.36960612-13
AGAGTAC532200.017.8933410-11
CTTATAC211700.017.7948321
CAGAGTA580050.017.52974310-11
CATGGGG334050.016.965764
Produced by FastQC (version 0.11.5)