##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138942_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15873562
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.439756684731506	34.0	31.0	34.0	31.0	34.0
2	32.56704783715212	34.0	31.0	34.0	31.0	34.0
3	32.61795531462945	34.0	31.0	34.0	31.0	34.0
4	36.144442627306965	37.0	35.0	37.0	35.0	37.0
5	36.05466618015541	37.0	35.0	37.0	35.0	37.0
6	36.071211111910486	37.0	35.0	37.0	35.0	37.0
7	36.02785978345629	37.0	35.0	37.0	35.0	37.0
8	36.022227525239764	37.0	35.0	37.0	35.0	37.0
9	37.74664262501384	39.0	38.0	39.0	35.0	39.0
10-11	37.68016419377075	39.0	37.0	39.0	35.0	39.0
12-13	37.66191948599817	39.0	37.0	39.0	35.0	39.0
14-15	39.08846703720312	41.0	38.0	41.0	36.0	41.0
16-17	39.063732450221316	41.0	38.0	41.0	35.0	41.0
18-19	39.06435984563515	41.0	38.5	41.0	35.0	41.0
20-21	39.0500851274512	41.0	39.0	41.0	35.0	41.0
22-23	39.03355912802692	41.0	39.0	41.0	35.0	41.0
24-25	38.99442352475515	41.0	39.0	41.0	35.0	41.0
26-27	38.934413235405714	41.0	38.0	41.0	35.0	41.0
28-29	38.87350728295098	40.0	38.0	41.0	35.0	41.0
30-31	38.79538157146962	40.0	38.0	41.0	34.5	41.0
32-33	38.70340454672176	40.0	38.0	41.0	34.0	41.0
34-35	38.62304991803386	40.0	38.0	41.0	34.0	41.0
36-37	38.599574793150225	40.0	38.0	41.0	34.0	41.0
38-39	38.54115854011478	40.0	38.0	41.0	34.0	41.0
40-41	38.53220233397394	40.0	38.0	41.0	34.0	41.0
42-43	38.48449113672662	40.0	38.0	41.0	34.0	41.0
44-45	38.39796877909931	40.0	38.0	41.0	33.0	41.0
46-47	38.3487741470701	40.0	38.0	41.0	33.0	41.0
48-49	38.29966590733209	40.0	38.0	41.0	33.0	41.0
50-51	38.22875803068715	40.0	38.0	41.0	33.0	41.0
52-53	38.103438197696505	40.0	37.0	41.0	33.0	41.0
54-55	37.977362139645884	40.0	37.0	41.0	33.0	41.0
56-57	37.84941358393621	40.0	37.0	41.0	33.0	41.0
58-59	37.73729917350302	40.0	36.0	41.0	32.0	41.0
60-61	37.59741479292184	39.5	36.0	41.0	32.0	41.0
62-63	37.39322126345471	39.0	35.5	41.0	32.0	41.0
64-65	37.19083006620684	39.0	35.0	41.0	32.0	41.0
66-67	36.962357873549635	38.5	35.0	41.0	31.5	41.0
68-69	36.63756512143566	37.5	35.0	40.0	31.0	41.0
70-71	36.31998319147931	37.0	35.0	40.0	31.0	41.0
72-73	36.01867608792567	37.0	35.0	39.0	31.0	41.0
74-75	35.75531793796595	36.0	35.0	39.0	31.0	41.0
76-77	35.46707428749527	36.0	34.5	39.0	31.0	40.5
78-79	35.193125015050526	35.0	34.0	37.5	31.0	39.5
80-81	34.91740867274599	35.0	34.0	37.0	31.0	39.0
82-83	34.62152729495398	35.0	34.0	36.5	31.0	39.0
84-85	34.38843053637267	35.0	34.0	36.0	31.0	37.5
86-87	34.22433413094447	35.0	34.0	36.0	31.0	37.0
88-89	34.07921802611655	35.0	34.0	36.0	31.0	37.0
90-91	33.94414923314663	35.0	34.0	35.0	31.0	36.0
92-93	33.88883285809975	35.0	34.0	35.0	31.0	36.0
94-95	33.83413828651308	35.0	34.0	35.0	31.0	36.0
96-97	33.82813125857264	35.0	34.0	35.0	31.0	36.0
98-99	33.97178613307446	35.0	34.0	35.0	31.0	36.0
100	31.184041195787877	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	27.0
19	93.0
20	164.0
21	514.0
22	2116.0
23	6876.0
24	18007.0
25	37961.0
26	68419.0
27	109109.0
28	159251.0
29	215469.0
30	276838.0
31	346500.0
32	431508.0
33	543046.0
34	714031.0
35	981964.0
36	1444262.0
37	2693124.0
38	4361239.0
39	3179861.0
40	283180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.33414176351848	20.700363283300874	14.615383743106936	24.350111210073706
2	14.630046456942292	20.998854185153775	41.655312156265786	22.71578720163815
3	18.42473088372472	25.917116477375636	30.267151053807883	25.39100158509176
4	12.73334869640475	18.254592132503088	38.22824391903972	30.783815252052438
5	13.135575173243891	37.71231622488225	35.71923337956195	13.432875222311914
6	29.926428842308194	38.02594109607158	18.558866219498217	13.488763842122012
7	26.318787175808428	32.5411775882439	23.04484651901067	18.095188716937006
8	23.89293593964606	37.67620021265548	20.991312472903058	17.439551374795396
9	24.968145145998108	17.258621599865233	21.464974276095056	36.3082589780416
10-11	24.1572352056946	27.022188536570603	28.724944887903824	20.095631369830972
12-13	24.80332936100363	24.33287713437124	29.0386782571379	21.82511524748723
14-15	21.919914116298685	25.830363562449982	26.553761509942415	25.69596081130892
16-17	21.622881921579243	29.044213047295663	27.28072658332329	22.0521784478018
18-19	21.622719496718254	28.087188547643972	29.01064452734336	21.279447428294414
20-21	22.282866197722385	27.193831182137195	29.013349952325086	21.509952667815334
22-23	22.11739236786599	27.270486081662167	28.790679823221033	21.82144172725081
24-25	21.986524024156516	27.404599585626094	28.871138060933838	21.737738329283548
26-27	21.937239944444972	27.564079867399904	28.78883864912786	21.709841539027263
28-29	21.78041000570257	27.469450048819215	28.85355831295071	21.8965816325275
30-31	22.15344960342649	27.387986111791296	28.799991911499163	21.65857237328305
32-33	21.981069504629673	27.430826679019816	28.838307356269393	21.749796460081118
34-35	22.041878664101873	27.52012606525176	28.85150724472251	21.586488025923863
36-37	22.028170605710958	27.404757371013744	28.700518404649948	21.866553618625346
38-39	21.86437520077096	27.38296819787986	28.857073562479922	21.89558303886926
40-41	22.068207311724453	27.25311253514257	28.801982862458487	21.87669729067449
42-43	21.988035653894748	27.32945056276932	28.68216907775945	22.000344705576477
44-45	21.996222252235643	27.530721311249152	28.46486277239234	22.008193664122867
46-47	22.151871533952864	27.4701406428579	28.06168444278203	22.31630338040721
48-49	22.099196091173688	27.584556790615988	28.048045677317162	22.26820144089316
50-51	21.99305501795574	27.691949615074773	28.04349538404121	22.271499982928272
52-53	22.087041876614627	27.692686454767035	28.045475994958522	22.174795673659816
54-55	22.067509028491124	27.697678152732628	27.95662413000204	22.278188688774204
56-57	22.16580251222843	27.788437152019434	27.802886692471144	22.242873643280987
58-59	21.968845374411963	27.945813603071297	28.030971546471388	22.054369476045352
60-61	22.1831130553449	28.038730113250875	27.784195915970944	21.99396091543328
62-63	22.02520479128249	28.186492820986974	27.692228014133846	22.09607437359669
64-65	21.958258298367202	28.162625693650007	27.707970973503453	22.17114503447934
66-67	22.026990926045105	28.10938887582562	27.61898644370648	22.244633754422793
68-69	22.016745475838235	27.961840301208923	27.73217998839218	22.28923423456066
70-71	22.064611808815187	27.752343315914075	27.737169588950362	22.445875286320383
72-73	22.14172751647266	27.66992167525013	27.72761784746889	22.46073296080832
74-75	22.096755265538135	27.828719497952726	27.705317026582684	22.36920820992646
76-77	22.232530407495418	27.752251175064536	27.579315998816668	22.435902418623378
78-79	22.109827830128847	27.767773872325975	27.649869921854563	22.472528375690615
80-81	22.153615020405116	27.784566511955124	27.670350015408896	22.391468452230864
82-83	22.142116610211307	27.84464674805805	27.712760232769785	22.300476408960858
84-85	22.300236522412856	27.83853135055045	27.636347068532658	22.224885058504032
86-87	22.11946225288527	27.878556053940002	27.751406117828502	22.250575575346225
88-89	22.161614681836376	27.81321585387077	27.73651375690076	22.288655707392095
90-91	22.17689261896288	27.904106199063673	27.698551331721255	22.22044985025219
92-93	22.249002075742595	27.877333310168368	27.726503476708057	22.147161137380984
94-95	22.120262384115915	27.93695746345506	27.806703727854615	22.13607642457441
96-97	22.14381748135455	28.033547882703136	27.903583229929513	21.919051406012805
98-99	23.128513985354786	29.885546994587305	28.136375263889686	18.849563756168227
100	22.268732103290155	30.878022507013057	21.590121067441252	25.263124322255532
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3072.0
1	2582.0
2	3193.5
3	4402.5
4	5674.5
5	9691.5
6	13415.0
7	16307.0
8	18032.0
9	17183.0
10	14387.0
11	11160.0
12	11357.5
13	16176.5
14	29596.5
15	49061.5
16	65509.5
17	75194.0
18	77956.0
19	75309.0
20	69528.0
21	63332.5
22	61029.0
23	67010.5
24	81642.0
25	103425.0
26	131163.0
27	163287.0
28	198246.0
29	237077.0
30	274898.5
31	311124.5
32	348140.0
33	380434.5
34	413493.5
35	447305.5
36	481205.5
37	515726.5
38	542648.0
39	561929.0
40	583359.5
41	604828.5
42	620099.5
43	633320.5
44	649217.0
45	667848.0
46	686484.5
47	696442.5
48	694205.0
49	680142.5
50	656708.5
51	632389.0
52	604652.0
53	571568.5
54	536480.5
55	497645.0
56	455605.5
57	410169.5
58	362475.0
59	315904.0
60	266262.0
61	217626.5
62	175116.5
63	137437.0
64	103721.0
65	77486.5
66	59451.5
67	47102.0
68	37093.0
69	28153.0
70	21386.0
71	16217.0
72	12244.5
73	9147.0
74	6811.0
75	5122.0
76	3844.5
77	2861.0
78	2194.5
79	1634.5
80	1206.0
81	886.0
82	667.5
83	488.0
84	345.5
85	245.5
86	177.5
87	133.0
88	92.5
89	55.5
90	36.5
91	29.0
92	22.5
93	15.5
94	8.0
95	4.5
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006394279998402375
3	2.078928472386979E-4
4	0.0
5	0.010823027622911607
6	0.012530268883568792
7	0.0
8	0.0
9	0.0
10-11	3.1498916248287564E-5
12-13	3.4648807873116316E-5
14-15	1.8899349748972537E-5
16-17	2.2049241373801293E-4
18-19	9.260681376996542E-4
20-21	0.0
22-23	1.291726344686337E-4
24-25	3.151419829660086E-6
26-27	3.1528811248420482E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.991191416596516E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.2580856726659975E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.006415853879164524
52-53	8.173591833165003E-5
54-55	3.430928378340824E-5
56-57	0.0
58-59	3.4872211478481337E-6
60-61	6.335807736112763E-5
62-63	9.910489453853885E-4
64-65	0.0013836424782542303
66-67	0.001140558810815003
68-69	0.010250399961384427
70-71	8.879936925808015E-4
72-73	5.307944909512623E-4
74-75	3.660213408554115E-4
76-77	6.858571007585808E-5
78-79	0.0
80-81	1.2451617683040335E-4
82-83	0.0
84-85	1.19271020294799E-5
86-87	1.8490409225717329E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	552.0
20-21	1965.0
22-23	3863.0
24-25	6578.0
26-27	9851.0
28-29	16486.0
30-31	39048.0
32-33	46778.0
34-35	64147.0
36-37	93978.0
38-39	104620.0
40-41	109997.0
42-43	124682.0
44-45	146661.0
46-47	148189.0
48-49	134035.0
50-51	114173.0
52-53	106752.0
54-55	112015.0
56-57	118876.0
58-59	131099.0
60-61	131704.0
62-63	125561.0
64-65	136600.0
66-67	145730.0
68-69	149587.0
70-71	142357.0
72-73	125844.0
74-75	127086.0
76-77	131932.0
78-79	137866.0
80-81	140321.0
82-83	134515.0
84-85	136090.0
86-87	142550.0
88-89	153888.0
90-91	163231.0
92-93	175356.0
94-95	224698.0
96-97	570747.0
98-99	1292460.0
100-101	9751094.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.04736734010946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.42823333755275	68.31924309190227
2	8.53022949093617	13.485843681309326
3	2.549243622825718	6.045329912788078
4	1.0751563655701917	3.399531207091358
5	0.5399040929623053	2.133899858240998
6	0.2892238042263332	1.3717428177709716
7	0.17018521543293524	0.941688526812803
8	0.11445272496098616	0.7237749274454071
9	0.0751050299764707	0.5343169404276011
>10	0.22263996255342008	2.6201564022487767
>50	0.0042412772756487156	0.2260817932908744
>100	0.0013363155410345666	0.15986069119745666
>500	3.250764063682689E-5	0.021070792113607184
>1k	1.625254537208249E-5	0.017459357360405298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.299783249657513E-6	0.0	0.0	0.0
3	0.0	6.299783249657513E-6	0.0	1.8899349748972537E-5	0.0
4	0.0	3.1498916248287564E-5	0.0	1.8899349748972537E-5	0.0
5	0.0	3.7798699497945074E-5	0.0	1.8899349748972537E-5	0.0
6	0.0	3.7798699497945074E-5	0.0	2.519913299863005E-5	0.0
7	0.0	3.7798699497945074E-5	0.0	2.519913299863005E-5	0.0
8	0.0	3.7798699497945074E-5	0.0	6.299783249657513E-5	0.0
9	0.0	1.070963152441777E-4	0.0	9.449674874486269E-5	0.0
10-11	1.2599566499315025E-5	1.070963152441777E-4	0.0	1.3544533986763652E-4	0.0
12-13	3.7798699497945074E-5	1.070963152441777E-4	0.0	1.732440393655816E-4	0.0
14-15	4.409848274760259E-5	1.6064447286626659E-4	0.0	2.2049241373801293E-4	0.0
16-17	5.984794087174637E-5	1.6379436449109533E-4	0.0	2.5514122161112926E-4	0.0
18-19	6.929761574623263E-5	1.8269371424006785E-4	0.0	3.1498916248287557E-4	0.0
20-21	7.559739899589015E-5	2.078928472386979E-4	0.0	3.4018829548150564E-4	0.0
22-23	8.189718224554767E-5	2.299420886124992E-4	0.0	4.0318612797808075E-4	0.0
24-25	1.070963152441777E-4	2.7089067973527304E-4	0.0	4.976828767229435E-4	0.0
26-27	1.2599566499315026E-4	2.8349024623458807E-4	0.0	6.677770244636963E-4	0.0
28-29	1.29145556617979E-4	2.8349024623458807E-4	0.0	0.0017355902852806446	0.0
30-31	1.4489501474212278E-4	3.212889457325331E-4	0.0	0.005688704274440733	0.0
32-33	1.574945812414378E-4	3.2758872898219066E-4	0.0	0.014262709277224606	0.0
34-35	1.795438226152391E-4	3.905865614787658E-4	0.0	0.025876359697968233	0.0
36-37	2.015930639890404E-4	4.504345023505121E-4	0.0	0.04348425388076098	0.0
38-39	2.015930639890404E-4	4.7878352697397096E-4	0.0	0.08377136776232078	0.0
40-41	2.3939176348698548E-4	5.29181792971231E-4	0.0	0.12410258012662816	0.0
42-43	2.3939176348698548E-4	5.54380925969861E-4	0.0	0.15194447219848956	0.0
44-45	2.5199132998630046E-4	6.425778914650662E-4	0.0	0.18691456901733838	0.0
46-47	2.645908964856155E-4	7.055757239616413E-4	0.0	0.22180906843719134	0.0
48-49	2.645908964856155E-4	7.055757239616413E-4	0.0	0.2502305405680212	0.0
50-51	2.645908964856155E-4	7.87472906207189E-4	0.0	0.2769164224135704	0.0
52-53	2.6774078811044424E-4	7.969225810816752E-4	0.0	0.34562185853433525	0.0
54-55	2.740405713601018E-4	8.063722559561615E-4	0.0	0.4171117988514487	0.0
56-57	2.771904629849305E-4	8.252716057051341E-4	0.0	0.5058442459228748	0.0
58-59	2.771904629849305E-4	8.252716057051341E-4	0.0	0.5602554738501667	0.0
60-61	2.960898127339031E-4	8.473208470789354E-4	0.0	0.5796651060423614	0.0
62-63	2.960898127339031E-4	8.567705219534216E-4	0.0	0.594463296895807	0.0
64-65	2.960898127339031E-4	9.890659701962295E-4	0.0	0.60167339882504	0.0
66-67	3.023895959835606E-4	9.985156450707158E-4	0.0	0.6081684753554369	0.0
68-69	3.212889457325331E-4	0.001020564886444517	0.0	0.6147202499350808	0.0
70-71	3.212889457325331E-4	0.0010426141278183183	0.0	0.620197911470658	0.0
72-73	3.212889457325331E-4	0.001058363585942462	0.0	0.6241510254598179	0.0
74-75	3.212889457325331E-4	0.0010835627189410921	0.0	0.6273576151338938	0.0
76-77	3.212889457325331E-4	0.001124511310063866	0.0	0.6304634082759748	0.0
78-79	3.212889457325331E-4	0.0011465605514376673	0.0	0.6325517864232363	0.0
80-81	3.212889457325331E-4	0.0011465605514376673	0.0	0.6331439660487042	0.0
82-83	3.2758872898219066E-4	0.0011528603346873246	0.0	0.6337770942652947	0.0
84-85	3.370384038566769E-4	0.0011623100095618111	0.0	0.6340416851617803	0.0
86-87	3.4018829548150564E-4	0.0011843592509356124	0.0	0.6341267322356507	0.0
88	3.4018829548150564E-4	0.00124105730018253	0.0	0.6341298821272755	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19080	0.0	47.230446	1
GTATCAA	33955	0.0	35.066364	1
ATCAACG	40050	0.0	28.988708	3
TCAACGC	40125	0.0	28.934433	4
CAACGCA	41125	0.0	28.148523	5
TATCAAC	42480	0.0	27.720264	2
AACGCAG	43075	0.0	26.929636	6
ACGCAGA	49595	0.0	23.33483	7
CGCAGAG	50140	0.0	23.047432	8
GTACATG	43055	0.0	19.87844	1
GTGGTAT	10080	0.0	19.820116	1
TACATGG	42535	0.0	19.534332	2
GCAGAGT	59240	0.0	19.507122	9
ACATGGG	42575	0.0	18.938513	3
TGGTATC	9640	0.0	18.529348	2
GAGTACT	36085	0.0	18.369606	12-13
AGAGTAC	53220	0.0	17.89334	10-11
CTTATAC	21170	0.0	17.794832	1
CAGAGTA	58005	0.0	17.529743	10-11
CATGGGG	33405	0.0	16.96576	4
>>END_MODULE