FastQCFastQC Report
Fri 27 May 2016
SRR2138941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16075007
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190600.051.09961
GTATCAA347800.036.7498741
TCAACGC419500.029.7557874
ATCAACG424750.029.4381523
CAACGCA433500.028.7653335
TATCAAC446400.028.203142
AACGCAG445050.028.0535246
ACGCAGA512150.024.3837937
CGCAGAG552900.022.6097628
GTGGTAT96350.021.3137321
GCAGAGT619900.020.214159
GTACATG441900.020.150531
TGGTATC92850.019.7310732
TACATGG443400.019.6787932
GAGTACT370100.019.55346112-13
ACATGGG447900.018.785463
AGAGTAC550800.018.47393410-11
CAGAGTA598550.018.38066310-11
CATGGGG355400.016.9738984
GTACTTT425500.016.9587414-15
Produced by FastQC (version 0.11.5)