##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138941_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16075007
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43207191138393	34.0	31.0	34.0	31.0	34.0
2	32.55874582200804	34.0	31.0	34.0	31.0	34.0
3	32.60827699795092	34.0	31.0	34.0	31.0	34.0
4	36.13886936410043	37.0	35.0	37.0	35.0	37.0
5	36.04775214094774	37.0	35.0	37.0	35.0	37.0
6	36.0669539366297	37.0	35.0	37.0	35.0	37.0
7	36.023476195064795	37.0	35.0	37.0	35.0	37.0
8	36.01783370918594	37.0	35.0	37.0	35.0	37.0
9	37.739020518000395	39.0	38.0	39.0	35.0	39.0
10-11	37.67216633249366	39.0	37.0	39.0	35.0	39.0
12-13	37.65539536001447	39.0	37.0	39.0	35.0	39.0
14-15	39.08137144201555	41.0	38.0	41.0	36.0	41.0
16-17	39.05660358343856	41.0	38.0	41.0	35.0	41.0
18-19	39.056001748552895	41.0	38.5	41.0	35.0	41.0
20-21	39.038091221223716	41.0	39.0	41.0	35.0	41.0
22-23	39.02251227831701	41.0	39.0	41.0	35.0	41.0
24-25	38.982829899187124	41.0	39.0	41.0	35.0	41.0
26-27	38.9245809886025	41.0	38.0	41.0	35.0	41.0
28-29	38.86461372068108	40.0	38.0	41.0	35.0	41.0
30-31	38.78660010629933	40.0	38.0	41.0	34.5	41.0
32-33	38.695868330926686	40.0	38.0	41.0	34.0	41.0
34-35	38.62116931361014	40.0	38.0	41.0	34.0	41.0
36-37	38.59639164940134	40.0	38.0	41.0	34.0	41.0
38-39	38.547091487884	40.0	38.0	41.0	34.0	41.0
40-41	38.51762224138993	40.0	38.0	41.0	34.0	41.0
42-43	38.461595513046454	40.0	38.0	41.0	34.0	41.0
44-45	38.3714295949758	40.0	38.0	41.0	33.0	41.0
46-47	38.319791996213254	40.0	38.0	41.0	33.0	41.0
48-49	38.261134898208134	40.0	38.0	41.0	33.0	41.0
50-51	38.19202197524733	40.0	37.5	41.0	33.0	41.0
52-53	38.05921262905341	40.0	37.0	41.0	33.0	41.0
54-55	37.92724455713111	40.0	37.0	41.0	33.0	41.0
56-57	37.790342022555066	40.0	36.5	41.0	32.5	41.0
58-59	37.66007181232197	40.0	36.0	41.0	32.0	41.0
60-61	37.51036355769155	39.0	36.0	41.0	32.0	41.0
62-63	37.2844395094144	39.0	35.0	41.0	32.0	41.0
64-65	37.06225923680778	39.0	35.0	41.0	32.0	41.0
66-67	36.817541627255444	38.0	35.0	40.5	31.0	41.0
68-69	36.483459673011424	37.0	35.0	40.0	31.0	41.0
70-71	36.15216924539047	37.0	35.0	39.5	31.0	41.0
72-73	35.845429030459435	36.0	35.0	39.0	31.0	41.0
74-75	35.584869788778434	36.0	35.0	39.0	31.0	41.0
76-77	35.3008322507238	35.5	34.0	38.5	31.0	40.0
78-79	35.03729663756113	35.0	34.0	37.0	31.0	39.0
80-81	34.7761265201261	35.0	34.0	37.0	31.0	39.0
82-83	34.49695288976042	35.0	34.0	36.5	30.0	39.0
84-85	34.278110778074804	35.0	34.0	36.0	30.0	37.0
86-87	34.12864209998099	35.0	34.0	36.0	30.0	37.0
88-89	33.999208857341706	35.0	34.0	35.0	30.0	37.0
90-91	33.87607358942414	35.0	34.0	35.0	30.0	36.0
92-93	33.82596020943819	35.0	34.0	35.0	30.5	36.0
94-95	33.77966153237152	35.0	34.0	35.0	31.0	36.0
96-97	33.78800278398106	35.0	34.0	35.0	31.0	36.0
98-99	33.931684377142105	35.0	34.0	35.0	31.0	36.0
100	31.12023324195453	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	24.0
19	115.0
20	178.0
21	486.0
22	2060.0
23	6808.0
24	18254.0
25	38685.0
26	70878.0
27	112988.0
28	163729.0
29	221852.0
30	285581.0
31	358289.0
32	446907.0
33	561159.0
34	736276.0
35	1022502.0
36	1527541.0
37	2838963.0
38	4439427.0
39	2967805.0
40	254498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94169290252875	20.25100206799288	14.569505319655537	24.237799709822834
2	15.034382000257185	20.875337339024608	41.03741560186495	23.052865058853254
3	18.767130266949582	25.318067210397672	29.96668176880809	25.948120753844655
4	13.132591481919729	17.841989120129153	37.57590898716249	31.449510410788623
5	13.531285930997678	37.406014417784775	35.34681615392641	13.715883497291138
6	30.845297931449572	37.31980932297637	18.15316489851984	13.681727847054226
7	27.033288383638027	32.22995797140244	22.635592009384506	18.10116163557503
8	24.48034392768849	37.302739588231596	20.393067324947356	17.82384915913256
9	25.255006109795165	17.11882302757318	21.177266050335156	36.448904812296504
10-11	24.636798701984812	26.80062702315262	28.171030994112627	20.39154328074994
12-13	25.190963422867274	24.20566653100054	28.54965652415917	22.053713521973016
14-15	22.23544046467415	25.523355153853284	26.255099803536623	25.986104577935944
16-17	21.93980037970214	28.744616202513868	26.908068280196005	22.407515137587982
18-19	22.000414560298275	27.839876458045087	28.60274454594108	21.556964435715564
20-21	22.7511396364287	26.905118618368835	28.670869191997557	21.67287255320491
22-23	22.556801949202963	26.825704771824487	28.51782044110125	22.0996728378713
24-25	22.3754743890962	26.924297444799173	28.61302911020074	22.087199055903888
26-27	22.207773470135887	27.044016345418044	28.603416751058298	22.144793433387775
28-29	22.20480679174786	27.04615487194123	28.572397557034616	22.176640779276298
30-31	22.466391176347905	26.795400407997228	28.579648078604624	22.158560337050247
32-33	22.340424300983088	26.93375918729991	28.495739724720387	22.23007678699662
34-35	22.457024934068183	27.018128618267205	28.47266062541448	22.052185822250127
36-37	22.488901857549614	26.936294698452024	28.337297478724153	22.237505965274206
38-39	22.32289727712272	27.08857406100617	28.41618036578967	22.172348296081438
40-41	22.545667445290746	26.9082770248889	28.29724469230161	22.248810837518747
42-43	22.387040933729427	27.08367984699241	28.122373463910698	22.406905755367465
44-45	22.375265246796815	27.12943590686092	28.04177810968905	22.453520736653207
46-47	22.530064225579956	27.133645297720122	27.75713320377707	22.579157272922856
48-49	22.559427092716522	27.32046203692759	27.61880730895992	22.50130356139597
50-51	22.52433302724749	27.355960559981597	27.59219689759468	22.527509515176234
52-53	22.711158452972352	27.310029264583243	27.50748346569954	22.471328816744865
54-55	22.626563231097965	27.27329130252329	27.497937844565985	22.60220762181276
56-57	22.60321010183399	27.43122580767845	27.440740591077905	22.524823499409656
58-59	22.51814703391748	27.37658931814446	27.57847671784173	22.526786930096325
60-61	22.599838083099417	27.351263399541743	27.510094261898534	22.538804255460313
62-63	22.52614516297521	27.464212362981	27.54210726787823	22.46753520616556
64-65	22.577020125796267	27.35675774254529	27.500676042075582	22.565546089582863
66-67	22.589992982787393	27.46365962481426	27.27098085883733	22.67536653356102
68-69	22.527101664669612	27.419336406340978	27.346886022729283	22.706675906260124
70-71	22.524376726808722	27.245966048665142	27.427134754610684	22.802522469915452
72-73	22.609158909161504	27.25090899752502	27.29829446860197	22.841637624711506
74-75	22.44493585980079	27.375550259758164	27.34307383791565	22.836440042525396
76-77	22.593148816791302	27.400755376383113	27.196082921934266	22.810012884891318
78-79	22.466490000895607	27.35761019937255	27.296129314408297	22.879770485323547
80-81	22.503807025295146	27.324264871650588	27.32887349524662	22.84305460780764
82-83	22.602469087364653	27.31253717128448	27.419940071006287	22.66505367034458
84-85	22.635431943973312	27.333003870703998	27.384636131348493	22.646928053974197
86-87	22.5129419156014	27.40419367460028	27.3861888183532	22.696675591445114
88-89	22.5769831072327	27.334282574409237	27.343685434123376	22.745048884234688
90-91	22.545862126798134	27.36926774754192	27.370495900083892	22.714374225576055
92-93	22.627045150937104	27.296686851946756	27.490836426912512	22.585431570203625
94-95	22.35949701228513	27.516162862759526	27.536735829733267	22.58760429522208
96-97	22.48000398894887	27.61065946777139	27.53500279566102	22.374333747618717
98-99	23.555795045940574	29.43523879788117	27.761712633142693	19.24725352303556
100	22.609503897939103	30.505536651570793	21.149412275950773	25.73554717453933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4867.0
1	3693.0
2	3509.0
3	4555.5
4	5482.5
5	8826.0
6	12280.0
7	15961.5
8	19218.5
9	19259.5
10	16202.0
11	12394.0
12	11970.0
13	16162.5
14	28539.5
15	46664.0
16	61904.0
17	70932.0
18	74217.5
19	72523.5
20	67444.0
21	61804.0
22	59432.5
23	63787.0
24	75626.5
25	95455.5
26	120407.0
27	147389.5
28	177663.5
29	213133.0
30	247813.0
31	281373.5
32	316915.0
33	350553.0
34	381121.5
35	409666.5
36	443248.5
37	477666.0
38	508103.5
39	533817.5
40	557235.5
41	579111.5
42	593288.5
43	607583.0
44	623542.0
45	643219.5
46	662099.5
47	677130.5
48	684261.0
49	679421.0
50	672635.0
51	664559.5
52	640982.5
53	603459.0
54	571114.0
55	541001.5
56	504161.5
57	462654.0
58	421374.0
59	370837.5
60	311670.5
61	261605.5
62	215482.5
63	173404.0
64	138050.0
65	103482.5
66	78298.5
67	60494.0
68	44277.0
69	32641.5
70	25124.5
71	19262.5
72	14439.0
73	10542.5
74	7778.0
75	5799.5
76	4234.0
77	3120.5
78	2424.5
79	1937.5
80	1510.0
81	1111.0
82	854.0
83	581.0
84	359.5
85	255.5
86	193.5
87	152.0
88	102.5
89	68.5
90	48.0
91	33.0
92	25.0
93	16.5
94	9.5
95	8.0
96	8.0
97	7.5
98	4.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006494553937052718
3	1.4930009050695902E-4
4	0.0
5	0.010351472941815827
6	0.012217724073152815
7	0.0
8	0.0
9	0.0
10-11	1.5552092761141565E-5
12-13	2.799376697005482E-5
14-15	3.110418552228313E-6
16-17	2.3950222852158013E-4
18-19	8.958005430417542E-4
20-21	0.0
22-23	1.2444459211869376E-4
24-25	0.0
26-27	6.227015308120812E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.9595092124602666E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.246788176180932E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.006110046421401629
52-53	6.35815174284636E-5
54-55	4.044101193117695E-5
56-57	3.395711304910392E-6
58-59	1.3692385096413903E-5
60-61	8.97504719407749E-5
62-63	0.00101956553215701
64-65	0.0015048264942067687
66-67	0.0012561540935195753
68-69	0.00988430224253678
70-71	9.479898523994217E-4
72-73	5.128822564200309E-4
74-75	4.3668670312474656E-4
76-77	1.1110206657991324E-4
78-79	0.0
80-81	1.2088178426348843E-4
82-83	0.0
84-85	3.084133187212243E-5
86-87	1.5581850447580863E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	574.0
20-21	2119.0
22-23	4318.0
24-25	6704.0
26-27	10048.0
28-29	16216.0
30-31	36234.0
32-33	44333.0
34-35	60126.0
36-37	87916.0
38-39	98898.0
40-41	102226.0
42-43	118477.0
44-45	140295.0
46-47	141357.0
48-49	127081.0
50-51	111014.0
52-53	103946.0
54-55	110291.0
56-57	116311.0
58-59	121458.0
60-61	118067.0
62-63	113091.0
64-65	121541.0
66-67	127835.0
68-69	130567.0
70-71	127794.0
72-73	120738.0
74-75	122674.0
76-77	128664.0
78-79	133714.0
80-81	136729.0
82-83	131329.0
84-85	132554.0
86-87	140052.0
88-89	150739.0
90-91	161009.0
92-93	175335.0
94-95	225807.0
96-97	588360.0
98-99	1340058.0
100-101	1.0088408E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.15308517385242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.97820275638317	72.27680784487606
2	8.144597987651728	13.382077043726792
3	2.1276108529889006	5.243693868672299
4	0.8057942073013528	2.647939205800997
5	0.3557165501231493	1.4611606020008014
6	0.1849152816917559	0.9114816532061842
7	0.11883380586458855	0.6833794642308613
8	0.06837351433165169	0.44936761172190404
9	0.04790020120029004	0.35416343781468856
>10	0.16114382424651486	2.06140661479743
>50	0.0050540277969206735	0.27010576465725245
>100	0.0018128636379982728	0.22898641900303537
>500	2.942603681266769E-5	0.01703934876608241
>1k	1.4700745189891791E-5	0.012391120725685928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.110418552228313E-5	0.0	0.0	0.0
3	0.0	3.110418552228313E-5	0.0	6.220837104456627E-6	0.0
4	0.0	7.465004525347951E-5	0.0	6.220837104456627E-6	0.0
5	0.0	8.709171946239278E-5	0.0	6.220837104456627E-6	0.0
6	4.354585973119639E-5	8.709171946239278E-5	0.0	1.2441674208913253E-5	0.0
7	4.354585973119639E-5	8.709171946239278E-5	0.0	1.2441674208913253E-5	0.0
8	4.354585973119639E-5	8.709171946239278E-5	0.0	1.2441674208913253E-5	6.220837104456627E-6
9	4.354585973119639E-5	1.9906678734261205E-4	0.0	3.110418552228313E-5	6.220837104456627E-6
10-11	5.2877115387881327E-5	1.9906678734261205E-4	0.0	4.9766696835653014E-5	9.33125565668494E-6
12-13	5.909795249233795E-5	2.0528762444706867E-4	0.0	6.220837104456626E-5	1.2441674208913253E-5
14-15	6.220837104456626E-5	3.265939479839729E-4	0.0	6.531878959679458E-5	1.8662511313369878E-5
16-17	8.709171946239278E-5	3.4214604074511446E-4	0.0	8.709171946239276E-5	2.4883348417826507E-5
18-19	8.709171946239278E-5	3.6391897061071265E-4	0.0	1.2441674208913252E-4	2.4883348417826507E-5
20-21	8.709171946239278E-5	3.8880231902853915E-4	0.0	1.5552092761141566E-4	2.4883348417826507E-5
22-23	8.709171946239278E-5	4.3856901586419216E-4	0.0	1.6174176471587227E-4	2.7993766970054816E-5
24-25	8.709171946239278E-5	4.914461312520734E-4	0.0	1.9595636879038373E-4	3.110418552228313E-5
26-27	9.020213801462108E-5	5.101086425654433E-4	0.0	3.1415227377505964E-4	3.110418552228313E-5
28-29	1.0575423077576265E-4	5.101086425654433E-4	0.0	9.580089140863205E-4	3.110418552228313E-5
30-31	1.0575423077576265E-4	6.65629570176859E-4	0.0	0.0031912894345862493	3.110418552228313E-5
32-33	1.1197506788021928E-4	6.780712443857723E-4	0.0	0.008279934186031769	3.7325022626739755E-5
34-35	1.2130632353690421E-4	7.465004525347952E-4	0.0	0.015185063371978625	3.7325022626739755E-5
36-37	1.3063757919358916E-4	8.180400792360463E-4	0.0	0.02596888449255419	3.7325022626739755E-5
38-39	1.3063757919358916E-4	8.584755204150144E-4	0.0	0.04978535934696638	3.7325022626739755E-5
40-41	1.3685841629804577E-4	9.611193326385487E-4	0.0	0.07299841300224628	4.354585973119639E-5
42-43	1.430792534025024E-4	9.953339367130601E-4	0.0	0.08947741049195188	4.66562782834247E-5
44-45	1.430792534025024E-4	0.0011228610973544211	0.0	0.10971690401130152	4.9766696835653014E-5
46-47	1.461896719547307E-4	0.001213063235369042	0.0	0.12984753288132317	4.9766696835653014E-5
48-49	1.4930009050695902E-4	0.0012161736539212705	0.0	0.1453871839682558	5.598753394010964E-5
50-51	1.4930009050695902E-4	0.0013281487218014896	0.0	0.16113523309818775	5.598753394010964E-5
52-53	1.4930009050695902E-4	0.0013405903960104029	0.0	0.2023140643111384	6.531878959679458E-5
54-55	1.5552092761141566E-4	0.0013779154186371427	0.0	0.24758309592026928	7.465004525347951E-5
56-57	1.648521832681006E-4	0.0014245716969205674	0.0	0.2980371952559647	7.465004525347951E-5
58-59	1.7729385747701384E-4	0.0014525654638906224	0.0	0.3324726390476844	7.465004525347951E-5
60-61	1.8973553168592712E-4	0.0014992217421740468	0.0	0.34399985020224255	7.465004525347951E-5
62-63	1.9906678734261205E-4	0.0015272155091441016	0.0	0.35691119761254225	7.465004525347951E-5
64-65	1.9906678734261205E-4	0.0017325031335911705	0.0	0.36335287443420705	7.465004525347951E-5
66-67	2.0528762444706867E-4	0.0017511656449045402	0.0	0.3689049715499346	7.465004525347951E-5
68-69	2.1461888010375363E-4	0.0017853802489790518	0.0	0.3744321853172443	7.776046380570783E-5
70-71	2.2706055431266685E-4	0.0018009323417401933	0.0	0.37889874635824417	8.087088235793614E-5
72-73	2.4261264707380842E-4	0.0018102635973968784	0.0	0.38233886927700866	8.709171946239278E-5
74-75	2.4261264707380842E-4	0.001866251131336988	0.0	0.38502627090613395	8.709171946239278E-5
76-77	2.5194390273049335E-4	0.0019533428507993804	0.0	0.3878069850918261	8.709171946239278E-5
78-79	2.550543212827217E-4	0.0019720053621127506	0.0	0.38957059241093955	8.709171946239278E-5
80-81	2.7371683259609154E-4	0.0019720053621127506	0.0	0.39012113649468394	9.33125565668494E-5
82-83	2.7371683259609154E-4	0.0019720053621127506	0.0	0.3907463306236818	1.1197506788021928E-4
84-85	2.7371683259609154E-4	0.001978226199217207	0.0	0.39095472866668113	1.1197506788021928E-4
86-87	2.7371683259609154E-4	0.002015551221843947	0.0	0.39099827452641234	1.1197506788021928E-4
88	2.7371683259609154E-4	0.002108863778410796	0.0	0.3910044953635168	1.1197506788021928E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19060	0.0	51.0996	1
GTATCAA	34780	0.0	36.749874	1
TCAACGC	41950	0.0	29.755787	4
ATCAACG	42475	0.0	29.438152	3
CAACGCA	43350	0.0	28.765333	5
TATCAAC	44640	0.0	28.20314	2
AACGCAG	44505	0.0	28.053524	6
ACGCAGA	51215	0.0	24.383793	7
CGCAGAG	55290	0.0	22.609762	8
GTGGTAT	9635	0.0	21.313732	1
GCAGAGT	61990	0.0	20.21415	9
GTACATG	44190	0.0	20.15053	1
TGGTATC	9285	0.0	19.731073	2
TACATGG	44340	0.0	19.678793	2
GAGTACT	37010	0.0	19.553461	12-13
ACATGGG	44790	0.0	18.78546	3
AGAGTAC	55080	0.0	18.473934	10-11
CAGAGTA	59855	0.0	18.380663	10-11
CATGGGG	35540	0.0	16.973898	4
GTACTTT	42550	0.0	16.95874	14-15
>>END_MODULE