FastQCFastQC Report
Fri 27 May 2016
SRR2138940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15501631
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189600.047.0611081
GTATCAA329000.035.7497331
ATCAACG390000.029.5165393
TCAACGC391250.029.4006084
CAACGCA399150.028.7657155
TATCAAC416550.027.870252
AACGCAG413850.027.8432166
CTTATAC110350.026.2628671
ACGCAGA475300.024.1692927
CGCAGAG481500.023.8492978
TATACAC171800.020.5619743
GTGGTAT96250.020.5276871
GCAGAGT575250.019.9772829
TGGTATC97350.018.6877232
GTACATG395850.018.3511621
GAGTACT368950.018.1649612-13
TACATGG387350.018.0329762
TTATACA180750.017.9531612
CAGAGTA565400.017.81173910-11
AGAGTAC521650.017.68810-11
Produced by FastQC (version 0.11.5)