##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138940_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15501631
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44115480493633	34.0	31.0	34.0	31.0	34.0
2	32.5707555546897	34.0	31.0	34.0	31.0	34.0
3	32.62039175103575	34.0	31.0	34.0	31.0	34.0
4	36.147755613586725	37.0	35.0	37.0	35.0	37.0
5	36.05904772213969	37.0	35.0	37.0	35.0	37.0
6	36.074363078310924	37.0	35.0	37.0	35.0	37.0
7	36.03224512310995	37.0	35.0	37.0	35.0	37.0
8	36.02727996815303	37.0	35.0	37.0	35.0	37.0
9	37.751545434154636	39.0	38.0	39.0	35.0	39.0
10-11	37.68637935582392	39.0	38.0	39.0	35.0	39.0
12-13	37.67052660458761	39.0	37.0	39.0	35.0	39.0
14-15	39.10007608231676	41.0	38.0	41.0	36.0	41.0
16-17	39.076444407688456	41.0	38.0	41.0	36.0	41.0
18-19	39.07867120562992	41.0	38.5	41.0	35.0	41.0
20-21	39.06175905095226	41.0	39.0	41.0	35.0	41.0
22-23	39.04688061649754	41.0	39.0	41.0	35.0	41.0
24-25	39.00914107617287	41.0	39.0	41.0	35.0	41.0
26-27	38.955013357902764	41.0	38.0	41.0	35.0	41.0
28-29	38.8980999090229	40.5	38.0	41.0	35.0	41.0
30-31	38.81964598555041	40.0	38.0	41.0	35.0	41.0
32-33	38.732044130172156	40.0	38.0	41.0	34.0	41.0
34-35	38.65604127778074	40.0	38.0	41.0	34.0	41.0
36-37	38.63616015540639	40.0	38.0	41.0	34.0	41.0
38-39	38.58270963811974	40.0	38.0	41.0	34.0	41.0
40-41	38.57784154943178	40.0	38.0	41.0	34.0	41.0
42-43	38.53567457307142	40.0	38.0	41.0	34.0	41.0
44-45	38.455527957503904	40.0	38.0	41.0	33.5	41.0
46-47	38.41143518790177	40.0	38.0	41.0	33.0	41.0
48-49	38.3652991037568	40.0	38.0	41.0	33.0	41.0
50-51	38.30902581330617	40.0	38.0	41.0	33.0	41.0
52-53	38.192192204828345	40.0	37.5	41.0	33.0	41.0
54-55	38.07242910057971	40.0	37.0	41.0	33.0	41.0
56-57	37.96209427839163	40.0	37.0	41.0	33.0	41.0
58-59	37.85234028123218	40.0	36.5	41.0	33.0	41.0
60-61	37.713398958153405	39.5	36.0	41.0	32.5	41.0
62-63	37.50967146304856	39.0	36.0	41.0	32.0	41.0
64-65	37.308106137896075	39.0	35.0	41.0	32.0	41.0
66-67	37.0824990857756	39.0	35.0	41.0	32.0	41.0
68-69	36.76224819105727	38.0	35.0	40.5	31.5	41.0
70-71	36.442335144157404	37.0	35.0	40.0	31.0	41.0
72-73	36.13864146154411	37.0	35.0	39.5	31.0	41.0
74-75	35.87288992464909	36.0	35.0	39.0	31.0	41.0
76-77	35.57616658943652	36.0	35.0	39.0	31.0	40.5
78-79	35.29518087017182	35.5	34.5	37.5	31.0	39.5
80-81	35.01003769727524	35.0	34.0	37.0	31.0	39.0
82-83	34.70435438584373	35.0	34.0	37.0	31.0	39.0
84-85	34.461864775989845	35.0	34.0	36.0	31.0	38.0
86-87	34.28850675680705	35.0	34.0	36.0	31.0	37.0
88-89	34.13567285222953	35.0	34.0	36.0	31.0	37.0
90-91	33.9922991443179	35.0	34.0	35.0	31.0	36.5
92-93	33.929724821173394	35.0	34.0	35.0	31.0	36.0
94-95	33.870012471019045	35.0	34.0	35.0	31.0	36.0
96-97	33.8600128427524	35.0	34.0	35.0	31.0	36.0
98-99	33.99261524821739	35.0	34.0	35.0	31.0	36.0
100	31.241959794854594	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	21.0
19	108.0
20	142.0
21	488.0
22	1833.0
23	6009.0
24	16234.0
25	34085.0
26	62443.0
27	101229.0
28	148049.0
29	201491.0
30	263139.0
31	332046.0
32	415249.0
33	523921.0
34	689150.0
35	945768.0
36	1387421.0
37	2579183.0
38	4295576.0
39	3204779.0
40	293266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.27880679136279	20.775988023453788	14.710419826146035	24.23478535903738
2	14.574107907802517	21.063227066753374	41.8093962571868	22.55326876825731
3	18.27085237571882	26.02753405567153	30.44240005372348	25.25921351488617
4	12.58552729064445	18.387039402499	38.594906561767594	30.43252674508895
5	12.968333680321841	37.84686135829517	35.963646012882684	13.221158948500303
6	29.745772619129653	38.33238879697294	18.658709334935434	13.263129248961977
7	26.100705145155374	32.702184692694594	23.26779033767479	17.929319824475243
8	23.699080438697067	37.78180502425841	21.112533255371645	17.406581281672878
9	24.889387445746838	17.346432772138623	21.585947956057012	36.17823182605753
10-11	23.92788252611847	27.146929020245587	28.99208724866223	19.93310120497371
12-13	24.6919017349725	24.49225497526744	29.24035273970239	21.575490550057673
14-15	21.743494071268636	25.982622472604493	26.803732897766235	25.470150558360636
16-17	21.414263219820466	29.16046165841945	27.56077102021334	21.86450410154674
18-19	21.419432442099335	28.266257338127843	29.267003427962013	21.04730679181081
20-21	22.11520832104897	27.364139049379453	29.278002763481325	21.242649866090247
22-23	21.84238669838143	27.414486437937963	29.093909661959028	21.64921720172158
24-25	21.733452195489566	27.509221056827244	29.190970408653953	21.566356339029237
26-27	21.724846021083295	27.65696881268766	29.09065323295194	21.527531933277107
28-29	21.573593926538752	27.65409774558268	29.193186952533008	21.57912137534556
30-31	21.92830659768476	27.53106400189369	29.04092610612608	21.49970329429547
32-33	21.71400292960518	27.530086217496212	29.052390490819302	21.7035203620793
34-35	21.698506024516632	27.712753733279282	29.13129362442023	21.457446617783855
36-37	21.83183351520293	27.582064808551536	28.95980014555015	21.626301530695375
38-39	21.664006947836942	27.610440835648458	29.081280152115518	21.64427206439908
40-41	21.89972748508559	27.52063043950215	28.924239449095612	21.655402626316643
42-43	21.88534536264082	27.588362422004884	28.766441270681714	21.759850944672582
44-45	21.88219693362729	27.80070193652105	28.50930548071055	21.807795649141106
46-47	21.9770840928645	27.70025448135011	28.194512408712548	22.128149017072843
48-49	22.01604150728245	27.77853897880316	28.171472131220632	22.03394738269376
50-51	21.88341892731314	27.90983319968045	28.18749991770143	22.01924795530498
52-53	21.949315738402696	27.897066959302318	28.188454502114624	21.96516280018036
54-55	22.00079123907645	27.864752341324035	28.091888517822454	22.042567901777062
56-57	22.090482864758613	27.995336549147936	27.917718907868366	21.99646167822509
58-59	21.91678590386153	28.11615383774772	28.16723220359114	21.799828054799605
60-61	22.099653151557654	28.18791951161125	27.893633722752465	21.818793614078636
62-63	21.935289243220144	28.345753153726537	27.833397725738536	21.88555987731478
64-65	21.912948499767815	28.27723908577944	27.862999151578038	21.94681326287471
66-67	21.989197931014676	28.19501699217935	27.820772550533015	21.995012526272962
68-69	21.983338627555842	28.08039475204532	27.918523679964885	22.017742940433955
70-71	21.959529196280418	27.855473293137003	27.979424507990146	22.205573002592438
72-73	22.043570423566145	27.887029110812232	27.94216569134224	22.12723477427938
74-75	21.93014713769935	27.96351916208078	27.97034585714897	22.135987843070904
76-77	22.001273656562777	27.944222459518397	27.8788107741136	22.175693109805227
78-79	21.950715905428936	27.911499523518103	27.909268385943438	22.228516185109523
80-81	21.93367630151374	27.98575979417448	27.931006930104978	22.149556974206806
82-83	21.983136330494304	27.95978226226566	28.009622328865063	22.047459078374974
84-85	22.145570183983057	27.923497178861368	27.911230828234896	22.01970180892068
86-87	21.930762809302053	27.96662826719733	27.998121505030632	22.104487418469983
88-89	22.00881505776934	27.940936268680872	27.95168542633779	22.098563247211995
90-91	22.039854077976404	28.011410121044644	27.93841795199788	22.010317848981074
92-93	22.045526029123973	27.95874476134857	27.983746591687343	22.01198261784011
94-95	21.903926828782367	28.04957888782729	28.041232871609946	22.0052614117804
96-97	21.94940933758385	28.1989917747482	28.09411460098083	21.75748428668712
98-99	22.952313952473556	30.045565906865214	28.323608588377848	18.678511552283382
100	22.02545106311703	31.100623834104805	21.748692080183343	25.125233022594823
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2931.0
1	2350.5
2	2883.0
3	4045.0
4	5248.5
5	9226.5
6	12842.5
7	15507.0
8	17180.0
9	16229.5
10	13435.0
11	10542.5
12	11102.0
13	16068.5
14	29401.5
15	48984.0
16	65467.0
17	75339.0
18	78628.0
19	76269.0
20	71073.0
21	65911.5
22	64347.5
23	70245.5
24	84418.0
25	106189.5
26	135016.0
27	168022.0
28	202490.5
29	240446.5
30	278064.5
31	314261.0
32	351989.0
33	389442.0
34	426345.0
35	461418.5
36	496898.0
37	529259.5
38	550633.0
39	566421.5
40	585749.0
41	605311.5
42	622273.0
43	637784.5
44	653877.5
45	671993.5
46	676132.5
47	668664.5
48	659304.5
49	643209.0
50	623756.5
51	599815.0
52	570347.0
53	536526.0
54	499130.0
55	458342.0
56	417439.0
57	377024.0
58	335788.0
59	292158.5
60	243490.0
61	196360.0
62	156288.0
63	122897.5
64	94602.0
65	72041.5
66	54947.5
67	43102.5
68	34052.0
69	26022.5
70	20061.5
71	15451.0
72	11843.5
73	9001.0
74	6737.5
75	5037.0
76	3793.0
77	2811.0
78	2066.0
79	1567.5
80	1179.0
81	867.0
82	663.0
83	448.0
84	299.5
85	236.5
86	184.0
87	137.0
88	94.5
89	59.0
90	35.5
91	25.5
92	22.0
93	16.5
94	9.0
95	6.5
96	6.0
97	4.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0063928756915965805
3	1.8062615475752196E-4
4	0.0
5	0.010308592689375718
6	0.012663183635321987
7	0.0
8	0.0
9	0.0
10-11	4.193107164013903E-5
12-13	1.2901868196965855E-5
14-15	6.4509340984829275E-6
16-17	2.580373639393171E-4
18-19	9.450618454277488E-4
20-21	0.0
22-23	1.4194842407246543E-4
24-25	0.0
26-27	1.291322397425258E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.2299097831826336E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.3376163851865636E-5
44-45	0.0
46-47	6.803040414822193E-6
48-49	0.0
50-51	0.0059390052381056
52-53	4.1914185943553813E-5
54-55	7.04199465343598E-5
56-57	7.1026387048565606E-6
58-59	1.7914687392287942E-5
60-61	1.0122096341100764E-4
62-63	0.0010065390759256495
64-65	0.0013429457341751873
66-67	0.0010365848312046066
68-69	0.009199914134134748
70-71	8.382700548808957E-4
72-73	5.74535572255049E-4
74-75	4.0202346139339706E-4
76-77	8.585739773096069E-5
78-79	0.0
80-81	1.473776360332425E-4
82-83	0.0
84-85	0.0
86-87	2.219855198845379E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	523.0
20-21	1797.0
22-23	3431.0
24-25	5954.0
26-27	9166.0
28-29	16165.0
30-31	37894.0
32-33	46056.0
34-35	62408.0
36-37	92722.0
38-39	106805.0
40-41	108806.0
42-43	124661.0
44-45	148195.0
46-47	146719.0
48-49	130376.0
50-51	116856.0
52-53	113169.0
54-55	119735.0
56-57	124020.0
58-59	124066.0
60-61	121817.0
62-63	119326.0
64-65	128996.0
66-67	136786.0
68-69	139326.0
70-71	131785.0
72-73	119830.0
74-75	121019.0
76-77	126509.0
78-79	130710.0
80-81	132204.0
82-83	128112.0
84-85	129354.0
86-87	136360.0
88-89	146187.0
90-91	154483.0
92-93	165755.0
94-95	211910.0
96-97	548068.0
98-99	1245444.0
100-101	9588126.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.63342242190285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.71164215988804	69.05144829015245
2	8.394970708572428	13.370404973104986
3	2.470506983529586	5.902047786470174
4	1.0754450502533102	3.425654799134655
5	0.5029044862396024	2.002400269529414
6	0.2858609245807334	1.3658450256631953
7	0.16357217566990845	0.9118068513114064
8	0.1080563932111809	0.6883920324778563
9	0.06739275692036054	0.48300442920141173
>10	0.21600979503284753	2.513256187490329
>50	0.0028253429630707284	0.1455904407116614
>100	7.504525212791368E-4	0.09859980410080534
>500	4.708136573443879E-5	0.024245075471546404
>1k	1.5689251870305833E-5	0.017304035180152954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2901868196965855E-5
2	0.0	6.4509340984829275E-6	0.0	0.0	1.2901868196965855E-5
3	0.0	1.2901868196965855E-5	0.0	0.0	1.2901868196965855E-5
4	0.0	2.580373639393171E-5	0.0	0.0	1.2901868196965855E-5
5	0.0	2.580373639393171E-5	0.0	0.0	1.2901868196965855E-5
6	2.580373639393171E-4	2.580373639393171E-5	0.0	0.0	1.9352802295448783E-5
7	2.580373639393171E-4	2.580373639393171E-5	0.0	0.0	1.9352802295448783E-5
8	2.644882980378E-4	2.580373639393171E-5	0.0	6.4509340984829275E-6	1.9352802295448783E-5
9	2.644882980378E-4	5.8058406886346344E-5	0.0	6.4509340984829275E-6	2.580373639393171E-5
10-11	2.838411003332488E-4	6.450934098482927E-5	0.0	1.2901868196965855E-5	2.580373639393171E-5
12-13	3.031939026286976E-4	6.773480803407074E-5	0.0	1.2901868196965855E-5	2.580373639393171E-5
14-15	3.096448367271805E-4	9.031307737876099E-5	0.0	1.2901868196965855E-5	4.193107164013903E-5
16-17	4.93496458533944E-4	9.676401147724392E-5	0.0	1.2901868196965855E-5	5.8058406886346344E-5
18-19	4.967219255831854E-4	1.0644041262496831E-4	0.0	1.9352802295448783E-5	6.450934098482927E-5
20-21	4.999473926324269E-4	1.1934228082193416E-4	0.0	1.9352802295448783E-5	7.09602750833122E-5
22-23	5.128492608293927E-4	1.2901868196965854E-4	0.0	1.9352802295448783E-5	8.063667623103659E-5
24-25	5.35427530174083E-4	1.2901868196965854E-4	0.0	2.580373639393171E-5	8.708761032951952E-5
26-27	5.612312665680147E-4	1.3869508311738294E-4	0.0	6.450934098482927E-5	9.353854442800245E-5
28-29	5.612312665680147E-4	1.419205501666244E-4	0.0	2.3223362754538538E-4	9.676401147724392E-5
30-31	5.612312665680147E-4	1.419205501666244E-4	0.0	9.289345101815415E-4	9.676401147724392E-5
32-33	5.709076677157391E-4	1.515969513143488E-4	0.0	0.0026255301780825514	9.676401147724392E-5
34-35	6.289660746020854E-4	1.935280229544878E-4	0.0	0.004670476287301639	1.0321494557572684E-4
36-37	6.741226132914659E-4	2.2578269344690244E-4	0.0	0.007728219049982547	1.0321494557572684E-4
38-39	6.837990144391903E-4	2.483609627915927E-4	0.0	0.012640605365977296	1.0321494557572684E-4
40-41	6.967008826361562E-4	2.8061563328400733E-4	0.0	0.01772394143558184	1.0321494557572684E-4
42-43	7.031518167346391E-4	3.193212378749049E-4	0.0	0.022278300909110788	1.0321494557572684E-4
44-45	7.192791519808465E-4	3.54801375416561E-4	0.0	0.027339058709370645	1.1289134672345123E-4
46-47	7.289555531285708E-4	3.8383057885973417E-4	0.0	0.033228761541285554	1.257932149204171E-4
48-49	7.354064872270537E-4	3.8705604590897567E-4	0.0	0.0386539971181097	1.2901868196965854E-4
50-51	7.934648941134001E-4	4.2253618345063174E-4	0.0	0.043714754918369556	1.2901868196965854E-4
52-53	7.99915828211883E-4	4.257616504998732E-4	0.0	0.048994844477977834	1.3869508311738292E-4
54-55	7.99915828211883E-4	4.289871175491147E-4	0.0	0.05423945390004445	1.6127335246207317E-4
56-57	7.99915828211883E-4	4.3866351869683906E-4	0.0	0.05957760186653908	1.7094975360979758E-4
58-59	8.031412952611244E-4	4.4188898574608055E-4	0.0	0.06527700214254874	1.8062615475752196E-4
60-61	8.35395965753539E-4	4.547908539430464E-4	0.0	0.07084738373658875	1.8062615475752196E-4
62-63	0.0010192475875603025	4.6446725509077076E-4	0.0	0.07584363219586378	1.9997895705297074E-4
64-65	0.0010192475875603025	5.031728596816684E-4	0.0	0.0806753818356275	1.9997895705297074E-4
66-67	0.0010515022580527172	5.096237937801513E-4	0.0	0.08579742350982295	1.9997895705297074E-4
68-69	0.0010579531921512	5.257511290263586E-4	0.0	0.0909839745250032	1.9997895705297074E-4
70-71	0.0010934333296928563	5.386529972233245E-4	0.0	0.09558349053722154	2.0642989115145368E-4
72-73	0.001203099209367066	5.418784642725659E-4	0.0	0.09905086761515611	2.1933175934841953E-4
74-75	0.0012063246764163075	5.515548654202902E-4	0.0	0.10193443515717798	2.1933175934841953E-4
76-77	0.0012418048139579635	5.870350029619463E-4	0.0	0.10454706346706356	2.1933175934841953E-4
78-79	0.0012643830833026538	5.934859370604293E-4	0.0	0.10628881567365395	2.1933175934841953E-4
80-81	0.0012708340174011367	5.999368711589123E-4	0.0	0.10682424320382804	2.1933175934841953E-4
82-83	0.0012837358855981025	6.031623382081537E-4	0.0	0.10759835529564599	2.3223362754538538E-4
84-85	0.0013063141549427928	6.063878052573952E-4	0.0	0.1078402653243391	2.3223362754538538E-4
86-87	0.0013192160231397586	6.063878052573952E-4	0.0	0.10791445106647166	2.3223362754538538E-4
88	0.0013546961606814149	6.257406075528439E-4	0.0	0.10792412746761937	2.3223362754538538E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18960	0.0	47.061108	1
GTATCAA	32900	0.0	35.749733	1
ATCAACG	39000	0.0	29.516539	3
TCAACGC	39125	0.0	29.400608	4
CAACGCA	39915	0.0	28.765715	5
TATCAAC	41655	0.0	27.87025	2
AACGCAG	41385	0.0	27.843216	6
CTTATAC	11035	0.0	26.262867	1
ACGCAGA	47530	0.0	24.169292	7
CGCAGAG	48150	0.0	23.849297	8
TATACAC	17180	0.0	20.561974	3
GTGGTAT	9625	0.0	20.527687	1
GCAGAGT	57525	0.0	19.977282	9
TGGTATC	9735	0.0	18.687723	2
GTACATG	39585	0.0	18.351162	1
GAGTACT	36895	0.0	18.16496	12-13
TACATGG	38735	0.0	18.032976	2
TTATACA	18075	0.0	17.953161	2
CAGAGTA	56540	0.0	17.811739	10-11
AGAGTAC	52165	0.0	17.688	10-11
>>END_MODULE