FastQCFastQC Report
Fri 27 May 2016
SRR2138939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37059544
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA443450.048.3381541
CTTATAC380450.043.3481331
GTATCAA791450.035.2797131
TATACAC550350.032.858993
TTATACA543850.031.3599622
TCAACGC935450.029.1099824
ATCAACG940400.029.0015353
CAACGCA956800.028.4252745
TATCAAC992600.027.7237152
AACGCAG995350.027.3760876
ACATCTC664350.025.1834268
ACACATC680300.024.6391566
TACACAT733350.024.5733155
ACGCAGA1144000.023.8013487
CGCAGAG1181200.023.0482068
CACATCT757950.021.8738927
GTGGTAT221600.021.7212811
CATCTCC771800.021.5249259
TGGTATC218200.020.5569842
ATACACA947500.020.5144634
Produced by FastQC (version 0.11.5)