##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37059544
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.442667643185246	34.0	31.0	34.0	31.0	34.0
2	32.57320824023091	34.0	31.0	34.0	31.0	34.0
3	32.622906423241474	34.0	31.0	34.0	31.0	34.0
4	36.14881872264807	37.0	35.0	37.0	35.0	37.0
5	36.05881683271656	37.0	35.0	37.0	35.0	37.0
6	36.075070972271	37.0	35.0	37.0	35.0	37.0
7	36.028772804112215	37.0	35.0	37.0	35.0	37.0
8	36.023860169461344	37.0	35.0	37.0	35.0	37.0
9	37.74929146456848	39.0	38.0	39.0	35.0	39.0
10-11	37.68108189620466	39.0	37.0	39.0	35.0	39.0
12-13	37.66216146642279	39.0	37.0	39.0	35.0	39.0
14-15	39.089494220436166	41.0	38.0	41.0	36.0	41.0
16-17	39.066011443637834	41.0	38.0	41.0	35.0	41.0
18-19	39.066960821212476	41.0	38.5	41.0	35.0	41.0
20-21	39.051376311258736	41.0	39.0	41.0	35.0	41.0
22-23	39.03581503583227	41.0	39.0	41.0	35.0	41.0
24-25	38.99334018049623	41.0	39.0	41.0	35.0	41.0
26-27	38.93041017177716	41.0	38.0	41.0	35.0	41.0
28-29	38.871950126871766	40.0	38.0	41.0	35.0	41.0
30-31	38.786756983199986	40.0	38.0	41.0	34.5	41.0
32-33	38.69428658046019	40.0	38.0	41.0	34.0	41.0
34-35	38.624209622650014	40.0	38.0	41.0	34.0	41.0
36-37	38.60310760839645	40.0	38.0	41.0	34.0	41.0
38-39	38.54138083182022	40.0	38.0	41.0	34.0	41.0
40-41	38.544440432379375	40.0	38.0	41.0	34.0	41.0
42-43	38.50420706352192	40.0	38.0	41.0	34.0	41.0
44-45	38.42371691621849	40.0	38.0	41.0	33.5	41.0
46-47	38.38113156406557	40.0	38.0	41.0	33.0	41.0
48-49	38.328076962832526	40.0	38.0	41.0	33.0	41.0
50-51	38.25896741328632	40.0	38.0	41.0	33.0	41.0
52-53	38.14517048921567	40.0	37.0	41.0	33.0	41.0
54-55	38.020587894313515	40.0	37.0	41.0	33.0	41.0
56-57	37.90447895255943	40.0	37.0	41.0	33.0	41.0
58-59	37.79995559746288	40.0	36.5	41.0	33.0	41.0
60-61	37.65774331699107	39.5	36.0	41.0	32.0	41.0
62-63	37.459081048880066	39.0	36.0	41.0	32.0	41.0
64-65	37.25750513173472	39.0	35.0	41.0	32.0	41.0
66-67	37.029828204050844	39.0	35.0	41.0	32.0	41.0
68-69	36.71038497404427	38.0	35.0	40.0	31.0	41.0
70-71	36.390560408666886	37.0	35.0	40.0	31.0	41.0
72-73	36.08805390110206	37.0	35.0	39.0	31.0	41.0
74-75	35.82220926232489	36.0	35.0	39.0	31.0	41.0
76-77	35.52593401154273	36.0	35.0	39.0	31.0	40.5
78-79	35.245676819065814	35.5	34.0	37.5	31.0	39.5
80-81	34.96172246791712	35.0	34.0	37.0	31.0	39.0
82-83	34.65918904617102	35.0	34.0	37.0	31.0	39.0
84-85	34.42188615389178	35.0	34.0	36.0	31.0	37.5
86-87	34.25299026913868	35.0	34.0	36.0	31.0	37.0
88-89	34.10715546687262	35.0	34.0	36.0	31.0	37.0
90-91	33.968856035104935	35.0	34.0	35.0	31.0	36.0
92-93	33.90977845359336	35.0	34.0	35.0	31.0	36.0
94-95	33.854393040238804	35.0	34.0	35.0	31.0	36.0
96-97	33.85093569569642	35.0	34.0	35.0	31.0	36.0
98-99	33.98568562112416	35.0	34.0	35.0	31.0	36.0
100	31.216565279115798	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	75.0
19	205.0
20	373.0
21	1289.0
22	4913.0
23	15943.0
24	40936.0
25	86057.0
26	154385.0
27	247804.0
28	361930.0
29	491321.0
30	638123.0
31	802149.0
32	1002322.0
33	1263195.0
34	1653758.0
35	2274182.0
36	3326927.0
37	6143974.0
38	1.033514E7
39	7516501.0
40	698036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.1092738755771	20.71605899953869	14.681044105669514	24.4936230192147
2	14.536852302317325	20.920922611894895	41.913356405089196	22.628868680698584
3	18.192621949217127	25.823979154797787	30.654273106979453	25.32912578900563
4	12.552609929577116	18.544529312071408	38.15177272553596	30.751088032815517
5	12.949031342453482	37.82366857201796	35.942895755251605	13.28440433027695
6	29.773114146904213	38.168677916402075	18.533047212084107	13.525160724609611
7	26.153333133294893	32.63921973783596	22.863937559512333	18.343509569356815
8	23.79392201911605	37.7030893850178	21.04013746094663	17.462851134919525
9	24.816203890690076	17.208298623426128	21.276365408057906	36.699132077825894
10-11	23.938042330166752	27.05429491708583	28.922692971157105	20.08496978159031
12-13	24.717750063209113	24.319702651382787	29.044452263695735	21.918095021712364
14-15	21.76263925564106	25.857105889603595	26.520750122640486	25.859504732114864
16-17	21.53529479819088	29.19409314152897	27.22136215548188	22.04924990479827
18-19	21.62751511172788	28.131733016945976	28.931306110962122	21.309445760364017
20-21	22.22525804751072	27.211127495122877	28.94067494113906	21.622939516227344
22-23	21.909073209248227	27.481286943146486	28.770959212034995	21.83868063557029
24-25	22.037120097297983	27.476158496831253	28.871149592800776	21.615571813069987
26-27	21.962836659706532	27.59738451609502	28.814088014426144	21.6256908097723
28-29	21.70322432673894	27.487486160731557	28.899557428550104	21.909732083979396
30-31	21.978388885056493	27.367285729720347	29.01756609398215	21.63675929124101
32-33	21.51081344365229	27.249086273395704	29.238439793383613	22.00166048956839
34-35	21.66302884140349	27.42498973525398	28.950043940411856	21.961937482930672
36-37	21.743660925436252	27.53288635411289	29.046306950908363	21.67714576954249
38-39	21.680173931898807	27.455554418259542	29.019843550009345	21.844428099832303
40-41	21.88670363062399	27.339994772566246	28.954166863382376	21.819134733427383
42-43	22.042738891799736	27.32354421999263	28.759513101912386	21.874203786295247
44-45	21.820098867655833	27.665318934923498	28.569405691621718	21.945176505798948
46-47	22.14217871280857	27.407001851175693	28.118466403069164	22.33235303294657
48-49	22.105625348820674	27.51059988455138	28.13017540976598	22.253599356861972
50-51	21.88248855854776	27.60207859172894	28.30153370127464	22.213899148448665
52-53	22.019555900538084	27.617784899459668	28.43761858937223	21.925040610630017
54-55	22.01111365599095	27.622736316202218	28.147345891465285	22.218804136341543
56-57	22.220278710431376	27.895009248696056	27.786870097800232	22.097841943072332
58-59	21.810775209586563	28.168122513224215	28.251447407503893	21.769654869685326
60-61	22.16519156002287	28.43756175377286	27.662276846562577	21.73496983964169
62-63	21.8875958333334	28.687652513024037	27.648959872556983	21.77579178108558
64-65	21.871242818954677	28.528887753119886	27.713240371922577	21.886629056002864
66-67	21.953231933376234	28.37882931080447	27.665386104242266	22.002552651577027
68-69	21.969021469510345	28.20997132767032	27.75904105655624	22.061966146263092
70-71	22.024862034784874	27.875752770541514	27.87333244807962	22.22605274659399
72-73	22.132175856123503	27.787235928824632	27.885515270668225	22.19507294438364
74-75	22.008523527698806	27.884856883876736	27.877343769898754	22.229275818525704
76-77	22.077548055512146	27.857295776877862	27.797770266404175	22.26738590120582
78-79	22.009864041754	27.82735861655448	27.848448542097547	22.314328799593966
80-81	22.04270913318897	27.89542746215673	27.83371306474507	22.228150339909224
82-83	22.097506125592503	27.852881637597687	27.91398051042841	22.135631726381398
84-85	22.17191601367756	27.831788377754773	27.86784779944615	22.128447809121514
86-87	22.025735689002214	27.901997514817932	27.88864448576675	22.1836223104131
88-89	22.094589932522418	27.81168997497864	27.899569879972596	22.194150212526345
90-91	22.081138783602093	27.900712258062388	27.90074274694936	22.11740621138616
92-93	22.123740751045055	27.833877721835705	27.935250502250597	22.107131024868647
94-95	21.98695745308678	27.98369160973818	27.92370654350683	22.105644393668204
96-97	22.020936475521825	28.101787047410156	28.001193376687628	21.8760831003804
98-99	23.05757085283885	29.89709418904905	28.30553381815724	18.73980113995486
100	22.097933601105787	30.96652071968823	21.712605951178908	25.22293972802708
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8455.0
1	6651.5
2	7366.0
3	10174.0
4	12961.5
5	21389.5
6	29211.0
7	35943.5
8	40975.5
9	40044.0
10	34092.0
11	27109.0
12	27862.5
13	39000.5
14	69569.0
15	114221.5
16	152144.0
17	175239.0
18	183176.0
19	178872.0
20	166944.0
21	153966.0
22	151297.0
23	166674.5
24	199504.0
25	249198.0
26	311678.5
27	383844.5
28	466288.5
29	556956.0
30	643306.0
31	725466.0
32	811914.0
33	895879.5
34	979651.0
35	1061806.0
36	1145017.5
37	1226271.5
38	1291593.0
39	1346543.0
40	1401502.5
41	1449791.0
42	1483073.0
43	1517339.5
44	1558880.0
45	1609607.5
46	1677897.0
47	1710156.5
48	1686169.0
49	1632275.5
50	1564545.0
51	1493631.0
52	1411815.0
53	1320504.0
54	1221064.0
55	1120440.0
56	1021955.0
57	920480.0
58	814814.5
59	702634.5
60	586302.5
61	482461.5
62	388634.5
63	305522.0
64	235890.0
65	177363.0
66	135117.5
67	104984.5
68	80142.0
69	60529.0
70	46734.5
71	36203.0
72	27926.5
73	21161.0
74	15926.5
75	11996.5
76	8849.5
77	6523.0
78	4841.0
79	3528.5
80	2556.5
81	1841.0
82	1420.5
83	1021.5
84	716.5
85	534.0
86	419.0
87	345.0
88	240.5
89	147.0
90	100.5
91	76.5
92	60.5
93	41.5
94	25.0
95	18.0
96	17.0
97	15.5
98	7.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0064814612937493235
3	2.1856717934791642E-4
4	0.0
5	0.011022801575756032
6	0.01184849980884816
7	0.0
8	0.0
9	0.0
10-11	4.317376382181065E-5
12-13	3.777704334408432E-5
14-15	1.0793440955452663E-5
16-17	2.19916359467348E-4
18-19	8.918080589442762E-4
20-21	0.0
22-23	1.2954678166390534E-4
24-25	0.0
26-27	1.2153583538797541E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.719276814332311E-5
36-37	0.0
38-39	5.51045143905026E-6
40-41	0.0
42-43	1.678079096146325E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.005991905171602887
52-53	6.743025271187619E-5
54-55	2.9566787284868175E-5
56-57	0.0
58-59	1.5058534178791725E-6
60-61	7.298124635882498E-5
62-63	9.473138432338378E-4
64-65	0.0012997478892357952
66-67	0.001008936810789655
68-69	0.009213090135070635
70-71	8.056955373376518E-4
72-73	4.632652894694468E-4
74-75	3.512015606478323E-4
76-77	7.293162593766865E-5
78-79	0.0
80-81	9.990248332343932E-5
82-83	0.0
84-85	1.2111507186233023E-5
86-87	1.5571511202914988E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1192.0
20-21	4273.0
22-23	8627.0
24-25	14550.0
26-27	23048.0
28-29	40836.0
30-31	96648.0
32-33	116890.0
34-35	158316.0
36-37	233175.0
38-39	267220.0
40-41	267774.0
42-43	308259.0
44-45	363861.0
46-47	361803.0
48-49	322949.0
50-51	290488.0
52-53	283733.0
54-55	301981.0
56-57	312000.0
58-59	315814.0
60-61	319847.0
62-63	322142.0
64-65	363672.0
66-67	380918.0
68-69	397381.0
70-71	348580.0
72-73	293013.0
74-75	298528.0
76-77	311246.0
78-79	320798.0
80-81	321524.0
82-83	311993.0
84-85	317187.0
86-87	332215.0
88-89	355399.0
90-91	372697.0
92-93	400437.0
94-95	509822.0
96-97	1300733.0
98-99	2946035.0
100-101	2.244194E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.38998102631064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06642495219222	67.46745419004738
2	8.85209761362398	13.878315279500669
3	2.4949159572650665	5.867292436567446
4	1.0721952101665444	3.361974487258262
5	0.5636005261129945	2.209031727420817
6	0.2960755674700979	1.3925614869801075
7	0.18531421325884997	1.0168744362886852
8	0.12828931141599165	0.8045277350222434
9	0.08058012011175801	0.5685006677992688
>10	0.2543493709988264	2.9486615909134315
>50	0.0045729532527590995	0.23799495168592405
>100	0.0015388704831551634	0.19819949954451008
>500	1.5203830734610142E-5	0.00962981049895428
>1k	3.012981706523608E-5	0.0389817004722539
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.095080716589497E-6
2	0.0	2.1586881910905326E-5	0.0	0.0	8.095080716589497E-6
3	0.0	2.1586881910905326E-5	0.0	5.396720477726332E-6	8.095080716589497E-6
4	0.0	6.20622854938528E-5	0.0	1.6190161433178995E-5	8.095080716589497E-6
5	2.698360238863166E-6	7.285572644930547E-5	0.0	1.6190161433178995E-5	8.095080716589497E-6
6	7.015736621044231E-5	7.285572644930547E-5	0.0	2.1586881910905326E-5	1.0793440955452663E-5
7	7.015736621044231E-5	7.285572644930547E-5	0.0	2.1586881910905326E-5	1.6190161433178995E-5
8	7.015736621044231E-5	8.364916740475813E-5	0.0	2.428524214976849E-5	1.888852167204216E-5
9	7.015736621044231E-5	1.5650489385406362E-4	0.0	6.476064573271598E-5	2.6983602388631658E-5
10-11	7.15065463298739E-5	1.5650489385406362E-4	0.0	8.63475276436213E-5	2.6983602388631658E-5
12-13	7.285572644930547E-5	1.578540739734952E-4	0.0	1.1468031015168455E-4	3.238032286635799E-5
14-15	8.095080716589497E-5	2.3610652090052698E-4	0.0	1.2007703062941087E-4	6.34114656132844E-5
16-17	1.1602949027111613E-4	2.52296682333706E-4	0.0	1.4301309265974776E-4	6.476064573271598E-5
18-19	1.1602949027111613E-4	2.7793110460290607E-4	0.0	1.8618685648155844E-4	7.555408668816864E-5
20-21	1.1602949027111613E-4	3.062638871109693E-4	0.0	2.280114401839375E-4	8.364916740475813E-5
22-23	1.1602949027111613E-4	3.345966696190326E-4	0.0	2.4689996185597965E-4	9.309342824077922E-5
24-25	1.1602949027111613E-4	3.723737129631169E-4	0.0	2.6309012328915867E-4	1.0523604931566346E-4
26-27	1.1602949027111613E-4	3.9396059487402217E-4	0.0	2.981688063943798E-4	1.0523604931566346E-4
28-29	1.1872785050997929E-4	4.047540358294748E-4	0.0	5.180851658617278E-4	1.0928358967395821E-4
30-31	1.1872785050997929E-4	4.843556628759383E-4	0.0	0.0013370374983566987	1.1333113003225297E-4
32-33	1.1872785050997929E-4	4.951491038313908E-4	0.0	0.003016766747049019	1.1333113003225297E-4
34-35	1.2142621074884246E-4	5.680048302806964E-4	0.0	0.005309023769963278	1.241245709877056E-4
36-37	1.2142621074884246E-4	6.368130163717071E-4	0.0	0.008424280665730803	1.241245709877056E-4
38-39	1.2142621074884246E-4	6.772884199546547E-4	0.0	0.013231409431265533	1.241245709877056E-4
40-41	1.2142621074884246E-4	7.447474259262338E-4	0.0	0.018180202109340578	1.268229312265688E-4
42-43	1.241245709877056E-4	7.771277487925918E-4	0.0	0.022875348924962485	1.2817211134600036E-4
44-45	1.3087047158486355E-4	8.756178975110973E-4	0.0	0.028242387440061325	1.3491801194315828E-4
46-47	1.3491801194315828E-4	9.44426083602108E-4	0.0	0.03386172263749387	1.5110817337633727E-4
48-49	1.3491801194315828E-4	9.457752637215397E-4	0.0	0.03921526935139839	1.5650489385406362E-4
50-51	1.497589932569057E-4	0.0010550588533954977	0.0	0.04468484555557402	1.5650489385406362E-4
52-53	1.5380653361520046E-4	0.0010658522943509505	0.0	0.04999252014541787	1.672983348095163E-4
54-55	1.5515571373463203E-4	0.0010766457353064032	0.0	0.05516797508355742	1.6999669504837945E-4
56-57	1.5650489385406362E-4	0.0010874391762618557	0.0	0.06062271030641932	1.7404423540667418E-4
58-59	1.5650489385406362E-4	0.0010874391762618557	0.0	0.06601808160402622	1.7809177576496893E-4
60-61	1.7134587516781102E-4	0.0011036293376950348	0.0	0.07122052014455439	1.807901360038321E-4
62-63	1.9698029743701108E-4	0.001122517859367077	0.0	0.07617740790334604	1.8618685648155844E-4
64-65	1.9832947755644268E-4	0.0012628325917879615	0.0	0.0810803284573604	1.8618685648155844E-4
66-67	2.1451963898962167E-4	0.0012979112748931826	0.0	0.08610737358236248	1.8618685648155844E-4
68-69	2.1856717934791642E-4	0.001326244057401246	0.0	0.09148925307877506	1.9293275707871633E-4
70-71	2.2126553958677959E-4	0.0013464817591927196	0.0	0.09623027201845764	2.0237701791473743E-4
72-73	2.455507817365481E-4	0.0013613227405064672	0.0	0.10007003863835992	2.1047209863132692E-4
74-75	2.455507817365481E-4	0.0013896555230145304	0.0	0.10316640701245541	2.1047209863132692E-4
76-77	2.455507817365481E-4	0.0014409243675529305	0.0	0.10603071640600867	2.131704588701901E-4
78-79	2.455507817365481E-4	0.0014706063301804253	0.0	0.10788718825034652	2.1586881910905326E-4
80-81	2.455507817365481E-4	0.00147465387053872	0.0	0.10855773076970401	2.2261471970621118E-4
82-83	2.4689996185597965E-4	0.0014854473114941729	0.0	0.10919454378607572	2.307098004228007E-4
84-85	2.4959832209484284E-4	0.001501637472927352	0.0	0.10944818964852887	2.3205898054223225E-4
86-87	2.509475022142744E-4	0.001520525994599394	0.0	0.10952374373521703	2.3205898054223225E-4
88	2.509475022142744E-4	0.0016082227023624467	0.0	0.10952914045569476	2.3475734078109542E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	44345	0.0	48.338154	1
CTTATAC	38045	0.0	43.348133	1
GTATCAA	79145	0.0	35.279713	1
TATACAC	55035	0.0	32.85899	3
TTATACA	54385	0.0	31.359962	2
TCAACGC	93545	0.0	29.109982	4
ATCAACG	94040	0.0	29.001535	3
CAACGCA	95680	0.0	28.425274	5
TATCAAC	99260	0.0	27.723715	2
AACGCAG	99535	0.0	27.376087	6
ACATCTC	66435	0.0	25.183426	8
ACACATC	68030	0.0	24.639156	6
TACACAT	73335	0.0	24.573315	5
ACGCAGA	114400	0.0	23.801348	7
CGCAGAG	118120	0.0	23.048206	8
CACATCT	75795	0.0	21.873892	7
GTGGTAT	22160	0.0	21.721281	1
CATCTCC	77180	0.0	21.524925	9
TGGTATC	21820	0.0	20.556984	2
ATACACA	94750	0.0	20.514463	4
>>END_MODULE