FastQCFastQC Report
Fri 27 May 2016
SRR2138938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9326608
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118100.048.6900441
GTATCAA203350.033.9044231
TCAACGC239650.028.1519764
ATCAACG240950.028.0175443
CAACGCA247650.027.2425615
TATCAAC254750.026.7168872
AACGCAG257250.026.33776
CTTATAC65150.023.1148011
ACGCAGA297850.022.7149777
GTGGTAT62250.022.5698491
CGCAGAG305700.022.1729498
TGGTATC58250.022.0976982
GCAGAGT357650.018.9875169
GAGTACT213650.018.72713712-13
TATACAC99850.018.4504933
GTACATG253750.018.3014091
TACATGG248900.018.2862192
ACATGGG250000.017.7330133
CAGAGTA351050.017.72152310-11
TATGCCG44150.017.1578642-43
Produced by FastQC (version 0.11.5)