##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2138938_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9326608 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39256812337347 34.0 31.0 34.0 31.0 34.0 2 32.52199416979892 34.0 31.0 34.0 31.0 34.0 3 32.57464364321949 34.0 31.0 34.0 31.0 34.0 4 36.11368002171851 37.0 35.0 37.0 35.0 37.0 5 36.023233527130124 37.0 35.0 37.0 35.0 37.0 6 36.045246996550084 37.0 35.0 37.0 35.0 37.0 7 36.003540729920246 37.0 35.0 37.0 35.0 37.0 8 35.99745105616104 37.0 35.0 37.0 35.0 37.0 9 37.715131481884946 39.0 38.0 39.0 35.0 39.0 10-11 37.64498582978935 39.0 37.0 39.0 35.0 39.0 12-13 37.627775017455434 39.0 37.0 39.0 35.0 39.0 14-15 39.04438971810545 41.0 38.0 41.0 35.0 41.0 16-17 39.01879107602679 41.0 38.0 41.0 35.0 41.0 18-19 39.02056723087321 41.0 38.5 41.0 35.0 41.0 20-21 39.00285922888642 41.0 39.0 41.0 35.0 41.0 22-23 38.98980368362524 40.0 39.0 41.0 35.0 41.0 24-25 38.9506306065943 41.0 38.0 41.0 35.0 41.0 26-27 38.89301983926751 40.0 38.0 41.0 35.0 41.0 28-29 38.83314119454515 40.0 38.0 41.0 35.0 41.0 30-31 38.75494174864521 40.0 38.0 41.0 34.5 41.0 32-33 38.663055632050884 40.0 38.0 41.0 34.0 41.0 34-35 38.58800877885788 40.0 38.0 41.0 34.0 41.0 36-37 38.56624439369884 40.0 38.0 41.0 34.0 41.0 38-39 38.5191467985112 40.0 38.0 41.0 34.0 41.0 40-41 38.50472869612854 40.0 38.0 41.0 34.0 41.0 42-43 38.45248381552069 40.0 38.0 41.0 34.0 41.0 44-45 38.36863030432984 40.0 38.0 41.0 33.0 41.0 46-47 38.32152806155543 40.0 38.0 41.0 33.0 41.0 48-49 38.2709274726172 40.0 38.0 41.0 33.0 41.0 50-51 38.21098632756575 40.0 38.0 41.0 33.0 41.0 52-53 38.08736494686681 40.0 37.0 41.0 33.0 41.0 54-55 37.967050062714065 40.0 37.0 41.0 33.0 41.0 56-57 37.84697716566138 40.0 37.0 41.0 33.0 41.0 58-59 37.73218091981632 40.0 36.0 41.0 32.0 41.0 60-61 37.58954031219527 39.5 36.0 41.0 32.0 41.0 62-63 37.37936390738986 39.0 35.5 41.0 32.0 41.0 64-65 37.169285827578605 39.0 35.0 41.0 32.0 41.0 66-67 36.938000361333266 38.5 35.0 41.0 31.5 41.0 68-69 36.609102082218335 37.5 35.0 40.0 31.0 41.0 70-71 36.28216338394087 37.0 35.0 40.0 31.0 41.0 72-73 35.97603340156053 36.5 35.0 39.0 31.0 41.0 74-75 35.71048457194576 36.0 35.0 39.0 31.0 41.0 76-77 35.42249761083992 36.0 34.5 39.0 31.0 40.0 78-79 35.145959516929324 35.0 34.0 37.0 31.0 39.0 80-81 34.869568899943246 35.0 34.0 37.0 31.0 39.0 82-83 34.57556460688191 35.0 34.0 36.5 31.0 39.0 84-85 34.34499030679294 35.0 34.0 36.0 30.0 37.0 86-87 34.18374543100124 35.0 34.0 36.0 31.0 37.0 88-89 34.043592097102845 35.0 34.0 35.5 31.0 37.0 90-91 33.9096059512632 35.0 34.0 35.0 30.0 36.0 92-93 33.85760261043719 35.0 34.0 35.0 31.0 36.0 94-95 33.80546382418235 35.0 34.0 35.0 31.0 36.0 96-97 33.80461315965239 35.0 34.0 35.0 31.0 36.0 98-99 33.94483772914616 35.0 34.0 35.0 31.0 36.0 100 31.194356392643037 33.0 29.0 35.0 24.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 19.0 19 39.0 20 100.0 21 289.0 22 1248.0 23 4120.0 24 10586.0 25 22682.0 26 40524.0 27 64562.0 28 94204.0 29 127804.0 30 165407.0 31 207302.0 32 258537.0 33 326457.0 34 426200.0 35 583837.0 36 857199.0 37 1584984.0 38 2561590.0 39 1822822.0 40 166096.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.57206006728277 20.519485755164148 14.764252984579173 24.144201192973906 2 14.920803012142631 20.871819880493064 41.42823075222288 22.77914635514143 3 18.75679911170068 25.37789283238261 30.111050281421303 25.754257774495404 4 12.979155980395015 18.028837493759788 37.91039571942983 31.081610806415366 5 13.289554601082923 37.581078494502016 35.55595111779993 13.57341578661514 6 30.48303574118979 37.77021592181146 18.218170279875768 13.528578057122978 7 26.749317651176074 32.45395324859799 22.625749897497567 18.170979202728365 8 24.04805691415357 37.68934000442605 20.61878230542122 17.643820775999163 9 24.99036091149108 17.334287020533083 21.279826492118033 36.3955255758578 10-11 24.367724005850576 26.947135685568707 28.49946928697033 20.185671021610386 12-13 24.93506748328384 24.26540801659253 28.869247827130256 21.930276672993372 14-15 22.000180665906655 25.787817274394794 26.47399925428405 25.73800280541451 16-17 21.70429451608765 28.971014617844126 27.210358608924246 22.114332257143975 18-19 21.742258492483778 27.980159601755272 28.99002109735066 21.28756080841029 20-21 22.406192590238376 27.153990934424645 28.99512430183457 21.444692173502414 22-23 22.13105607670625 27.116883100169908 28.82733505840967 21.924725764714164 24-25 22.027078213518447 27.18526634694385 28.939695879643423 21.847959559894285 26-27 21.915311392012732 27.337885669585045 28.87845515485166 21.86834778355057 28-29 21.806438143119177 27.34663220910213 28.99189929335358 21.855030354425114 30-31 22.0821131008001 27.17178086501501 28.792810767714606 21.95329526647028 32-33 21.97582492356344 27.289015972931992 28.835995884124994 21.899163219379577 34-35 22.08571890072838 27.457223476878177 28.7337348292524 21.723322793141037 36-37 22.020904384848446 27.30340678590434 28.69107081274179 21.98461801650543 38-39 21.867275437865047 27.411087350037196 28.81730742167963 21.904329790418128 40-41 22.183690596167253 27.30047967277124 28.653441774054418 21.86238795700709 42-43 22.101410377348788 27.37280253488415 28.48672359874201 22.03906348902505 44-45 22.098343059855523 27.551435365552763 28.293240152650313 22.056981421941398 46-47 22.276257971525382 27.46758025173608 27.971828215882276 22.284333560856258 48-49 22.21516613989549 27.570207430295323 27.95447871998185 22.260147709827336 50-51 22.1086793287343 27.6948686286965 27.95318831775982 22.243263724809378 52-53 22.238818122053853 27.694910386806683 27.884002553111674 22.182268938027786 54-55 22.238217966624816 27.591035598962492 27.910523044959945 22.260223389452747 56-57 22.30819109793532 27.732897336259636 27.757634099286495 22.20127746651855 58-59 22.170543685478314 27.865751869278 27.893328581014593 22.070375864229096 60-61 22.268199807557913 27.884449095971643 27.776674511985348 22.070676584485092 62-63 22.21853855497337 27.96678497403981 27.708733604035057 22.105942866951757 64-65 22.147762480447078 27.924670349608387 27.749782984907824 22.177784185036707 66-67 22.23482541453263 27.876851509177136 27.639582774938344 22.24874030135189 68-69 22.173118576698762 27.822611328417086 27.745560956516346 22.258709138367802 70-71 22.172960205814483 27.566641483061193 27.824812103333585 22.435586207790738 72-73 22.266713017040182 27.564490092928644 27.750090598288953 22.418706291742218 74-75 22.096113455760662 27.725339941777506 27.77736620833386 22.401180394127973 76-77 22.23718421102418 27.68554624529982 27.682482565337068 22.39478697833893 78-79 22.18813914723895 27.713678972274558 27.64176338269341 22.45641849779308 80-81 22.205307773596576 27.69718595131167 27.67014007343718 22.427366201654575 82-83 22.210634105018993 27.68014353277577 27.777972365586105 22.331249996619132 84-85 22.344273399801782 27.688085525161533 27.715075787001247 22.252565288035438 86-87 22.150439634658625 27.7351760871033 27.77867963853421 22.335704639703863 88-89 22.244543820432803 27.704779740726305 27.70794789236789 22.34272854647301 90-91 22.21506663025108 27.764709966499783 27.764051085258878 22.256172317990256 92-93 22.26359404452614 27.72584772532628 27.784061640527746 22.22649658961984 94-95 22.15405281822583 27.824134853008246 27.80784490981952 22.213967418946407 96-97 22.177036738835568 27.952631144503044 27.849062523742717 22.021269592918674 98-99 23.1626264906581 29.79560166360875 28.14177846441263 18.899993381320524 100 22.320438366363334 30.866118332690572 21.502132396850723 25.311310904095375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1834.0 1 1492.5 2 1734.0 3 2409.5 4 3117.5 5 5185.5 6 7140.5 7 8947.0 8 10443.5 9 10355.5 10 8894.0 11 7135.5 12 7138.5 13 9733.0 14 17510.0 15 28800.5 16 38127.5 17 43667.5 18 45598.5 19 44567.5 20 41641.5 21 38283.5 22 36690.0 23 39669.5 24 47686.0 25 60233.0 26 77173.0 27 95612.5 28 113814.5 29 134833.5 30 156241.5 31 177277.5 32 198557.0 33 218966.0 34 240251.5 35 259913.0 36 281416.0 37 302650.0 38 317934.0 39 330680.0 40 346398.5 41 360583.0 42 368850.5 43 377618.5 44 386882.0 45 396415.5 46 405925.0 47 410801.5 48 409651.5 49 402657.5 50 393156.0 51 379599.5 52 360833.5 53 338855.0 54 315202.5 55 291740.5 56 268616.0 57 244096.0 58 218572.0 59 192213.5 60 163226.5 61 135133.0 62 108639.0 63 85217.0 64 66145.0 65 51330.5 66 39924.5 67 31053.0 68 23974.5 69 18509.5 70 14300.5 71 10746.5 72 8093.0 73 5939.0 74 4381.5 75 3317.5 76 2472.5 77 1794.0 78 1345.0 79 1038.0 80 784.5 81 574.0 82 439.0 83 314.5 84 209.5 85 149.0 86 112.0 87 85.0 88 50.0 89 27.5 90 17.5 91 15.0 92 15.0 93 10.5 94 7.0 95 4.5 96 2.0 97 1.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.006669091270910066 3 2.3588425717045253E-4 4 0.0 5 0.00989641678946944 6 0.012319591431311363 7 0.0 8 0.0 9 0.0 10-11 4.824905260304711E-5 12-13 2.144402337913205E-5 14-15 5.361005844783012E-6 16-17 2.9485532146306565E-4 18-19 8.738439526996309E-4 20-21 0.0 22-23 7.507009536100593E-5 24-25 0.0 26-27 1.0732084877054309E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 4.3289895710853366E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 2.7798676204800186E-5 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.005996719210533362 52-53 8.165098287370634E-5 54-55 3.5293368182005075E-5 56-57 5.936347866185696E-6 58-59 1.1984662508308368E-5 60-61 7.258764489325654E-5 62-63 0.0010133204018999637 64-65 0.001355363326702321 66-67 0.0011574861317914956 68-69 0.009073914294835811 70-71 9.025085479317524E-4 72-73 4.8147663105023534E-4 74-75 4.407338399914498E-4 76-77 9.819477417885929E-5 78-79 0.0 80-81 1.2038210081345528E-4 82-83 0.0 84-85 1.3669414104989984E-5 86-87 1.6585917436546946E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 339.0 20-21 1153.0 22-23 2275.0 24-25 3784.0 26-27 5772.0 28-29 10176.0 30-31 24435.0 32-33 29668.0 34-35 40215.0 36-37 60386.0 38-39 68419.0 40-41 68589.0 42-43 78271.0 44-45 92788.0 46-47 91990.0 48-49 82940.0 50-51 74587.0 52-53 71903.0 54-55 76591.0 56-57 78550.0 58-59 78563.0 60-61 75932.0 62-63 74155.0 64-65 79530.0 66-67 83292.0 68-69 84798.0 70-71 80481.0 72-73 74050.0 74-75 75685.0 76-77 78792.0 78-79 81561.0 80-81 82612.0 82-83 79001.0 84-85 79758.0 86-87 83913.0 88-89 90494.0 90-91 95236.0 92-93 101803.0 94-95 130873.0 96-97 334251.0 98-99 745569.0 100-101 5673428.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.13754895800373 #Duplication Level Percentage of deduplicated Percentage of total 1 89.53432826972627 76.22733256485799 2 7.346336413790477 12.508981521821047 3 1.7868390987185006 4.563813037416648 4 0.6181528709048926 2.105120812407834 5 0.29005814534914154 1.234741977516515 6 0.1477603079497611 0.7547970271273496 7 0.08175616136715567 0.48723634337102695 8 0.054801217905618045 0.37325130979182297 9 0.03233136437200144 0.24773518053902974 >10 0.10580061766079256 1.3466081792829272 >50 0.0014700763261347497 0.08095667908760615 >100 3.257580682084565E-4 0.05063387019387429 >500 3.9697861039524046E-5 0.018791496586311483 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0722011689566024E-5 0.0 0.0 0.0 3 0.0 1.0722011689566024E-5 0.0 0.0 0.0 4 0.0 6.433207013739614E-5 0.0 0.0 0.0 5 0.0 6.433207013739614E-5 0.0 0.0 0.0 6 0.0 6.433207013739614E-5 0.0 0.0 0.0 7 0.0 6.433207013739614E-5 0.0 0.0 0.0 8 0.0 1.0722011689566025E-4 0.0 0.0 0.0 9 0.0 2.251622454808865E-4 0.0 0.0 0.0 10-11 0.0 2.251622454808865E-4 0.0 0.0 0.0 12-13 0.0 2.251622454808865E-4 0.0 0.0 0.0 14-15 0.0 3.431043740661128E-4 0.0 0.0 0.0 16-17 0.0 3.431043740661128E-4 0.0 0.0 0.0 18-19 0.0 3.7527040913481086E-4 0.0 0.0 0.0 20-21 0.0 4.28880467582641E-4 0.0 0.0 0.0 22-23 0.0 5.468225961678672E-4 0.0 1.0722011689566024E-5 0.0 24-25 5.361005844783012E-6 5.843496370813484E-4 0.0 3.216603506869807E-5 0.0 26-27 1.0722011689566024E-5 6.111546663052634E-4 0.0 5.897106429261313E-5 0.0 28-29 1.0722011689566024E-5 6.218766779948295E-4 0.0 3.4846537991089577E-4 0.0 30-31 1.0722011689566024E-5 6.862087481322255E-4 0.0 0.0012491143618344417 0.0 32-33 1.0722011689566024E-5 6.915697539770086E-4 0.0 0.002959275226320223 0.0 34-35 1.0722011689566024E-5 7.773458474935368E-4 0.0 0.005489669985057805 0.0 36-37 1.0722011689566024E-5 8.47038923475716E-4 0.0 0.008861742661426319 0.0 38-39 1.0722011689566024E-5 8.68482946854848E-4 0.0 0.014260275547122812 0.0 40-41 1.0722011689566024E-5 0.0010078690988192063 0.0 0.020012634818574983 0.0 42-43 1.0722011689566024E-5 0.0010453961397326873 0.0 0.02491795516655144 0.0 44-45 1.0722011689566024E-5 0.0011847822916970458 0.0 0.03107238987636234 0.0 46-47 1.0722011689566024E-5 0.001286641402747923 0.0 0.03762353901868718 0.0 48-49 1.0722011689566024E-5 0.001286641402747923 0.0 0.043665392605757636 0.0 50-51 1.0722011689566024E-5 0.0014367495664018473 0.0 0.04942311288305459 0.0 52-53 1.0722011689566024E-5 0.0014474715780914134 0.0 0.054896699850578046 0.0 54-55 1.0722011689566024E-5 0.0014581935897809793 0.0 0.060783084268149795 0.0 56-57 1.0722011689566024E-5 0.0014796376131601115 0.0 0.06667482969156632 0.0 58-59 1.0722011689566024E-5 0.0014796376131601115 0.0 0.07265771221434417 0.0 60-61 1.0722011689566024E-5 0.0014903596248496773 0.0 0.07834037840981416 0.0 62-63 1.0722011689566024E-5 0.0015600527008318567 0.0 0.08392654650007805 0.0 64-65 1.0722011689566024E-5 0.0016994388527962149 0.0 0.08982901393518415 0.0 66-67 1.0722011689566024E-5 0.0017101608644857808 0.0 0.09524899084425978 0.0 68-69 1.0722011689566024E-5 0.0017530489112440452 0.0 0.10050813757799192 0.0 70-71 1.0722011689566024E-5 0.00177985394046796 0.0 0.10490416237071398 0.0 72-73 1.0722011689566024E-5 0.001801297963847092 0.0 0.10827087404123771 0.0 74-75 1.0722011689566024E-5 0.0018441860106053563 0.0 0.1110854021097488 0.0 76-77 1.0722011689566024E-5 0.0018977960690531863 0.0 0.11389456917241508 0.0 78-79 1.0722011689566024E-5 0.0019138790865875354 0.0 0.11583525328822655 0.0 80-81 1.0722011689566024E-5 0.0019192400924323183 0.0 0.11644640795453182 0.0 82-83 1.0722011689566024E-5 0.0019299621041218844 0.0 0.11708972865590578 0.0 84-85 1.0722011689566024E-5 0.0019621281391905823 0.0 0.11738458397736884 0.0 86-87 1.0722011689566024E-5 0.0019835721625697145 0.0 0.11744891604750624 0.0 88 1.0722011689566024E-5 0.0020479042327071107 0.0 0.11744891604750624 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11810 0.0 48.690044 1 GTATCAA 20335 0.0 33.904423 1 TCAACGC 23965 0.0 28.151976 4 ATCAACG 24095 0.0 28.017544 3 CAACGCA 24765 0.0 27.242561 5 TATCAAC 25475 0.0 26.716887 2 AACGCAG 25725 0.0 26.3377 6 CTTATAC 6515 0.0 23.114801 1 ACGCAGA 29785 0.0 22.714977 7 GTGGTAT 6225 0.0 22.569849 1 CGCAGAG 30570 0.0 22.172949 8 TGGTATC 5825 0.0 22.097698 2 GCAGAGT 35765 0.0 18.987516 9 GAGTACT 21365 0.0 18.727137 12-13 TATACAC 9985 0.0 18.450493 3 GTACATG 25375 0.0 18.301409 1 TACATGG 24890 0.0 18.286219 2 ACATGGG 25000 0.0 17.733013 3 CAGAGTA 35105 0.0 17.721523 10-11 TATGCCG 4415 0.0 17.15786 42-43 >>END_MODULE