FastQCFastQC Report
Fri 27 May 2016
SRR2138937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6567431
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106700.046.3804631
CTTATAC67050.042.1484951
GTATCAA196800.038.726631
TATACAC97050.032.8089523
ATCAACG237150.031.7060953
TCAACGC239650.031.4130674
CAACGCA243400.030.9305085
TATCAAC245700.030.7589052
AACGCAG248200.030.3666886
TTATACA109050.027.5960122
ACGCAGA283300.026.5289067
CGCAGAG285450.026.3442278
TGCGCGA3803.6379788E-1223.45687594
GCAGAGT327300.022.8755479
ACATCTC126200.022.6662718
ACACATC132200.021.863276
TACACAT150650.021.449495
TACATGG242750.021.2467962
CACATCT139750.020.4379167
GTACATG265200.020.0750331
Produced by FastQC (version 0.11.5)