FastQCFastQC Report
Fri 27 May 2016
SRR2138936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7350101
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94650.048.044071
CTTATAC63900.037.844751
GTATCAA170600.035.2781751
TCAACGC202250.029.0294574
ATCAACG204300.028.7383653
CAACGCA207050.028.2552725
TATACAC98350.028.2508473
TATCAAC214700.027.641522
AACGCAG214400.027.3033926
TTATACA104200.025.0984882
ACGCAGA248500.023.5377867
TGGTATC45850.023.2180122
CGCAGAG253450.023.0780818
GTGGTAT50150.022.4808161
ACATCTC111750.021.9713468
ACACATC112900.021.749476
TACACAT128750.020.8641225
GCAGAGT296600.019.777149
TACATGG217400.019.7603662
GTACATG224150.019.6327551
Produced by FastQC (version 0.11.5)