##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138936_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7350101
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.428882813991265	34.0	31.0	34.0	31.0	34.0
2	32.55735070851407	34.0	31.0	34.0	31.0	34.0
3	32.60889571449427	34.0	31.0	34.0	31.0	34.0
4	36.138838500314485	37.0	35.0	37.0	35.0	37.0
5	36.04900300009483	37.0	35.0	37.0	35.0	37.0
6	36.06734900649665	37.0	35.0	37.0	35.0	37.0
7	36.02286621639621	37.0	35.0	37.0	35.0	37.0
8	36.01580304814859	37.0	35.0	37.0	35.0	37.0
9	37.74087689407261	39.0	38.0	39.0	35.0	39.0
10-11	37.67108717281572	39.0	37.0	39.0	35.0	39.0
12-13	37.6537061191404	39.0	37.0	39.0	35.0	39.0
14-15	39.07499237085314	41.0	38.0	41.0	36.0	41.0
16-17	39.04651663970332	41.0	38.0	41.0	35.0	41.0
18-19	39.049584964886876	41.0	38.5	41.0	35.0	41.0
20-21	39.03790895673316	41.0	39.0	41.0	35.0	41.0
22-23	39.025581498201106	41.0	39.0	41.0	35.0	41.0
24-25	38.98507352222873	41.0	39.0	41.0	35.0	41.0
26-27	38.920498315310105	41.0	38.0	41.0	35.0	41.0
28-29	38.85709765919924	40.0	38.0	41.0	35.0	41.0
30-31	38.779640938988834	40.0	38.0	41.0	34.5	41.0
32-33	38.68839449048997	40.0	38.0	41.0	34.0	41.0
34-35	38.61249697445474	40.0	38.0	41.0	34.0	41.0
36-37	38.586984718243684	40.0	38.0	41.0	34.0	41.0
38-39	38.527454961636444	40.0	38.0	41.0	34.0	41.0
40-41	38.52466672629795	40.0	38.0	41.0	34.0	41.0
42-43	38.4852005304911	40.0	38.0	41.0	34.0	41.0
44-45	38.40460020737571	40.0	38.0	41.0	33.5	41.0
46-47	38.36005452187837	40.0	38.0	41.0	33.0	41.0
48-49	38.30845096027501	40.0	38.0	41.0	33.0	41.0
50-51	38.238725589510715	40.0	38.0	41.0	33.0	41.0
52-53	38.12475804593944	40.0	37.0	41.0	33.0	41.0
54-55	38.00041189610087	40.0	37.0	41.0	33.0	41.0
56-57	37.88626863861072	40.0	37.0	41.0	33.0	41.0
58-59	37.77706236442082	40.0	36.0	41.0	33.0	41.0
60-61	37.638358495282475	39.5	36.0	41.0	32.0	41.0
62-63	37.43727068267929	39.0	35.5	41.0	32.0	41.0
64-65	37.23782984009824	39.0	35.0	41.0	32.0	41.0
66-67	37.0135573448548	39.0	35.0	41.0	32.0	41.0
68-69	36.693896639293214	38.0	35.0	40.0	31.0	41.0
70-71	36.378188643857825	37.0	35.0	40.0	31.0	41.0
72-73	36.07697114436127	37.0	35.0	39.0	31.0	41.0
74-75	35.81657295043057	36.0	35.0	39.0	31.0	41.0
76-77	35.527038025893305	36.0	35.0	39.0	31.0	40.5
78-79	35.24890698644096	35.5	34.0	37.5	31.0	39.5
80-81	34.968883182517956	35.0	34.0	37.0	31.0	39.0
82-83	34.669360876125594	35.0	34.0	37.0	31.0	39.0
84-85	34.43305047921003	35.0	34.0	36.0	31.0	37.5
86-87	34.26362721723689	35.0	34.0	36.0	31.0	37.0
88-89	34.11733640962145	35.0	34.0	36.0	31.0	37.0
90-91	33.97675431210543	35.0	34.0	35.0	31.0	36.0
92-93	33.91611270046298	35.0	34.0	35.0	31.0	36.0
94-95	33.86121809860836	35.0	34.0	35.0	31.0	36.0
96-97	33.8521829437368	35.0	34.0	35.0	31.0	36.0
98-99	33.98537719807999	35.0	34.0	35.0	31.0	36.0
100	31.207991426249134	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	12.0
19	38.0
20	74.0
21	267.0
22	963.0
23	3077.0
24	8245.0
25	17151.0
26	31044.0
27	49675.0
28	72244.0
29	98441.0
30	127376.0
31	160192.0
32	200373.0
33	251709.0
34	329138.0
35	452745.0
36	662289.0
37	1227788.0
38	2019838.0
39	1500692.0
40	136729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.27067111050583	20.708477339290983	14.740668189457532	24.280183360745657
2	14.57406159421325	21.016803728193256	41.783145493664364	22.625989183929125
3	18.324433666644534	25.994164967820794	30.48046499579325	25.200936369741424
4	12.62744552761928	18.419583622048187	38.174128491567664	30.77884235876487
5	12.961408790983311	37.86195161124849	35.744402871855826	13.432236725912366
6	29.751771037564108	38.12317302114795	18.574412560348215	13.550643380939725
7	26.17904162133282	32.71610825483895	22.897345220154115	18.207504903674113
8	23.915821020690736	37.667332734611406	20.96289561191064	17.453950632787222
9	24.95621488738726	17.286551572556622	21.3182376677545	36.43899587230162
10-11	24.06495942807818	27.083947412656066	28.688395953494755	20.162697205771
12-13	24.75166997773363	24.399082842160745	29.009585994635717	21.839661185469907
14-15	21.858692826125793	25.940187760685195	26.5913556834117	25.609763729777317
16-17	21.540437550309825	29.210326335881625	27.28212976715017	21.96710634665838
18-19	21.53880858045419	28.118964948512797	29.064532453300707	21.27769401773231
20-21	22.271585755410023	27.181284051285314	29.119922772016558	21.427207421288106
22-23	21.9121713351544	27.330655322850316	28.963235178549645	21.793938163445638
24-25	21.816217678900525	27.453698955433165	29.045264290729637	21.68481907493668
26-27	21.79761416960092	27.609427953306348	29.023438473445477	21.569519403647256
28-29	21.47828515667507	27.428727797843578	29.20432794401536	21.88865910146599
30-31	21.919759607772484	27.241658886541202	29.283491392724354	21.55509011296196
32-33	21.62049488466323	27.585660288252097	29.03107530727098	21.762769519813695
34-35	21.646734005512837	27.65100684049255	29.242796545902962	21.459462608091652
36-37	21.92364285605303	27.47807652974116	28.902673192498153	21.69560742170766
38-39	21.701055658851555	27.404839815485687	29.074800056183136	21.819304469479626
40-41	21.841015977113702	27.34619686585331	29.00458862599037	21.80819853104262
42-43	21.928588987564602	27.375532975991	28.912990344975686	21.782887691468712
44-45	21.848355977599116	27.605466856095656	28.65146228780784	21.894714878497393
46-47	22.109278304586404	27.46434589083387	28.187127690273776	22.23924811430596
48-49	22.00957345623538	27.541797360823107	28.24342974103616	22.205199441905354
50-51	21.844210084715325	27.734890442575676	28.28487114713613	22.13602832557287
52-53	21.940622087110476	27.821754382632864	28.291155261815444	21.946468268441215
54-55	21.94602269538404	27.816564927413594	28.10108412282465	22.136328254377716
56-57	22.117341715449093	28.00117943365584	27.833169048960016	22.048309801935055
58-59	21.82138044440465	28.219448943969056	28.199733996935144	21.759436614691154
60-61	22.094768039226988	28.295427186318644	27.841381132338377	21.76842364211599
62-63	21.868890717354432	28.506744780375254	27.770412019713177	21.85395248255714
64-65	21.898633687019878	28.367382011499124	27.824485771379486	21.909498530101512
66-67	22.004031667371308	28.263759211484015	27.73122969988075	22.000979421263935
68-69	21.926716523988564	28.122731744630453	27.905051604242082	22.045500127138908
70-71	21.968636832237216	27.812358384542406	28.00968931485969	22.209315468360693
72-73	22.056982028919332	27.735668837477984	27.977526814670895	22.229822318931785
74-75	21.87578989184232	27.907457974017312	27.969237688149956	22.24751444599041
76-77	22.044712706557277	27.848800313787503	27.858092503073422	22.248394476581794
78-79	21.974711951079883	27.890686541840875	27.888440711563778	22.246160795515458
80-81	21.97326621699681	27.854838988820283	27.958234611419066	22.213660182763846
82-83	21.97166415792911	27.857780090796176	28.060789033982864	22.109766717291855
84-85	22.142295136971704	27.850314854634856	27.943250339336227	22.064139669057212
86-87	21.96897635865658	27.91309669089186	27.978487716119727	22.139439234331828
88-89	22.006985309204133	27.87797265834362	27.969737494509967	22.14530453794228
90-91	22.01247363279238	27.932171002284743	27.940222196625413	22.115133168297465
92-93	22.025649281307217	27.89174155934991	28.02261876050098	22.059990398841897
94-95	21.897529738437065	28.037000564613056	27.982688563660634	22.082781133289238
96-97	22.000504038664754	28.116574566996476	28.072860633500333	21.810060760838432
98-99	22.968870288966794	29.989823829935634	28.32739680036228	18.713909080735284
100	22.058840429940435	31.070134715240737	21.747217215284117	25.123807639534707
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1515.0
1	1243.0
2	1504.0
3	2081.5
4	2690.0
5	4620.0
6	6362.5
7	7767.5
8	8853.5
9	8675.5
10	7358.5
11	5846.0
12	5967.0
13	8130.0
14	14459.0
15	23787.0
16	31689.5
17	36483.0
18	37790.0
19	36558.5
20	34065.5
21	31304.5
22	30607.5
23	33488.5
24	40356.5
25	51121.0
26	65321.0
27	81255.5
28	97348.0
29	115339.5
30	131670.0
31	147189.0
32	164993.0
33	181834.0
34	198735.5
35	214675.0
36	230159.5
37	246081.5
38	257626.5
39	265211.5
40	274112.5
41	283071.0
42	289599.5
43	296843.0
44	308947.0
45	324267.5
46	329233.5
47	326858.0
48	321380.5
49	311558.5
50	303523.5
51	293552.0
52	280519.5
53	265163.0
54	247325.0
55	226656.5
56	205252.5
57	184447.5
58	164448.5
59	143411.5
60	120532.0
61	98034.0
62	77183.5
63	60387.0
64	46771.0
65	35880.0
66	27545.0
67	21549.5
68	17018.5
69	12916.0
70	9895.0
71	7577.5
72	5777.0
73	4372.0
74	3251.5
75	2472.0
76	1849.5
77	1362.0
78	1055.0
79	800.5
80	578.0
81	407.5
82	281.0
83	183.5
84	120.0
85	85.0
86	62.5
87	52.0
88	32.5
89	17.0
90	12.0
91	8.5
92	8.5
93	7.5
94	6.0
95	5.5
96	5.0
97	4.5
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006557733016185764
3	1.7686831786393139E-4
4	0.0
5	0.009618915440753807
6	0.01194541408342552
7	0.0
8	0.0
9	0.0
10-11	7.482890371166328E-5
12-13	4.081576566090724E-5
14-15	0.0
16-17	2.789077320161995E-4
18-19	9.591704930313203E-4
20-21	0.0
22-23	6.803999036825896E-5
24-25	6.805748952254949E-6
26-27	6.8087985200940245E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.0990496517661178E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.11918838193115E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0060232563886231496
52-53	1.0386605789286334E-4
54-55	2.9932838194301535E-5
56-57	0.0
58-59	7.6266713850340304E-6
60-61	9.248150061715988E-5
62-63	8.329222563599258E-4
64-65	0.0012264071882555473
66-67	0.0010253534130915216
68-69	0.00944003694962333
70-71	9.113757844036424E-4
72-73	6.168129897854947E-4
74-75	4.5670279019663384E-4
76-77	1.3418305873469233E-4
78-79	0.0
80-81	1.2851610422450429E-4
82-83	0.0
84-85	8.755933348784638E-6
86-87	1.9477386311824102E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	249.0
20-21	891.0
22-23	1633.0
24-25	2916.0
26-27	4715.0
28-29	8528.0
30-31	20233.0
32-33	24458.0
34-35	33091.0
36-37	48461.0
38-39	56313.0
40-41	55705.0
42-43	63281.0
44-45	74756.0
46-47	74505.0
48-49	66439.0
50-51	60201.0
52-53	57206.0
54-55	60663.0
56-57	63613.0
58-59	67126.0
60-61	66400.0
62-63	62327.0
64-65	68043.0
66-67	71774.0
68-69	74521.0
70-71	67725.0
72-73	58510.0
74-75	58763.0
76-77	61393.0
78-79	63649.0
80-81	64259.0
82-83	61934.0
84-85	62099.0
86-87	65867.0
88-89	70768.0
90-91	74244.0
92-93	79247.0
94-95	100209.0
96-97	255817.0
98-99	579838.0
100-101	4437731.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.24381295479635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.94108343597952	74.92735996977596
2	7.620361952993719	12.83936694031699
3	1.9530790606647956	4.936044812177231
4	0.7000472682689489	2.358986045102619
5	0.32638858239227414	1.3748109342817942
6	0.16017560445376472	0.809628219691464
7	0.09534123842046129	0.562233661946039
8	0.05653828040639233	0.3810400255473555
9	0.03596010871755604	0.272647500537234
>10	0.10923978769793645	1.3564474222272447
>50	0.0014383789014943216	0.07883456325155568
>100	2.685959298520559E-4	0.048855769685407044
>500	5.180345591496475E-5	0.02954509629853411
>1k	2.5901717418050166E-5	0.02419903916047998
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.721051044060483E-5	0.0
4	0.0	0.0	0.0	4.081576566090724E-5	0.0
5	0.0	0.0	0.0	4.081576566090724E-5	0.0
6	0.0	1.3605255220302414E-5	0.0	4.081576566090724E-5	0.0
7	0.0	1.3605255220302414E-5	0.0	4.081576566090724E-5	0.0
8	0.0	2.721051044060483E-5	0.0	5.442102088120966E-5	0.0
9	0.0	1.4965780742332656E-4	0.0	1.6326306264362897E-4	0.0
10-11	0.0	1.4965780742332656E-4	0.0	1.7686831786393139E-4	0.0
12-13	0.0	1.4965780742332656E-4	0.0	1.7686831786393139E-4	0.0
14-15	0.0	2.108814559146874E-4	0.0	1.904735730842338E-4	0.0
16-17	0.0	2.1768408352483863E-4	0.0	2.3128933874514107E-4	0.0
18-19	0.0	2.516972215755947E-4	0.0	2.789077320161995E-4	0.0
20-21	0.0	2.857103596263507E-4	0.0	2.9931561484665317E-4	0.0
22-23	0.0	3.3332875289740916E-4	0.0	3.3332875289740916E-4	0.0
24-25	0.0	3.809471461684676E-4	0.0	3.673418909481652E-4	0.0
26-27	0.0	3.9455240138877004E-4	0.0	4.1496028421922365E-4	0.0
28-29	0.0	3.9455240138877004E-4	0.0	7.074732714557256E-4	0.0
30-31	0.0	4.489734222699797E-4	0.0	0.0016870516473174995	0.0
32-33	0.0	4.625786774902821E-4	0.0	0.003857089854955735	0.0
34-35	0.0	5.306049535917941E-4	0.0	0.0067618118444903	0.0
36-37	0.0	5.986312296933062E-4	0.0	0.010659717465106942	0.0
38-39	0.0	5.986312296933062E-4	0.0	0.01638752991285426	0.0
40-41	0.0	6.394469953542136E-4	0.0	0.022176566009092936	0.0
42-43	0.0	6.734601334049695E-4	0.0	0.02772751013897632	0.0
44-45	1.3605255220302414E-5	7.686969199470864E-4	0.0	0.033931506519434225	0.0
46-47	1.3605255220302414E-5	8.163153132181449E-4	0.0	0.040353186983416965	0.0
48-49	1.3605255220302414E-5	8.163153132181449E-4	0.0	0.04593134162374095	0.0
50-51	1.3605255220302414E-5	9.659731206414714E-4	0.0	0.051938061803504465	0.0
52-53	1.3605255220302414E-5	9.795783758617738E-4	0.0	0.05754342695426906	0.0
54-55	1.3605255220302414E-5	9.863810034719252E-4	0.0	0.06348892348554122	0.0
56-57	1.3605255220302414E-5	0.0010067888863023787	0.0	0.06976774876971079	0.0
58-59	1.3605255220302414E-5	0.00101359151391253	0.0	0.07618262660608337	0.0
60-61	1.3605255220302414E-5	0.0010203941415226811	0.0	0.08238662298654129	0.0
62-63	1.3605255220302414E-5	0.001047604651963286	0.0	0.08835933002825402	0.0
64-65	1.3605255220302414E-5	0.0011972624593866125	0.0	0.09402591882750999	0.0
66-67	1.3605255220302414E-5	0.0012040650869967638	0.0	0.09974692864764716	0.0
68-69	1.3605255220302414E-5	0.0012448808526576709	0.0	0.10563120153042796	0.0
70-71	1.3605255220302414E-5	0.0012584861078779735	0.0	0.11081480376936317	0.0
72-73	1.3605255220302414E-5	0.0012652887354881247	0.0	0.1146991041347595	0.0
74-75	1.3605255220302414E-5	0.0012924992459287295	0.0	0.11769906291083619	0.0
76-77	1.3605255220302414E-5	0.0013401176391997878	0.0	0.12065820592125197	0.0
78-79	1.3605255220302414E-5	0.0013605255220302415	0.0	0.12255613902448415	0.0
80-81	1.3605255220302414E-5	0.0013605255220302415	0.0	0.1230799413504658	0.0
82-83	1.3605255220302414E-5	0.0013605255220302415	0.0	0.123943875056955	0.0
84-85	1.3605255220302414E-5	0.001374130777250544	0.0	0.12426359855463211	0.0
86-87	1.3605255220302414E-5	0.001374130777250544	0.0	0.12446767738293664	0.0
88	1.3605255220302414E-5	0.0014285517981317535	0.0	0.1245697167970889	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9465	0.0	48.04407	1
CTTATAC	6390	0.0	37.84475	1
GTATCAA	17060	0.0	35.278175	1
TCAACGC	20225	0.0	29.029457	4
ATCAACG	20430	0.0	28.738365	3
CAACGCA	20705	0.0	28.255272	5
TATACAC	9835	0.0	28.250847	3
TATCAAC	21470	0.0	27.64152	2
AACGCAG	21440	0.0	27.303392	6
TTATACA	10420	0.0	25.098488	2
ACGCAGA	24850	0.0	23.537786	7
TGGTATC	4585	0.0	23.218012	2
CGCAGAG	25345	0.0	23.078081	8
GTGGTAT	5015	0.0	22.480816	1
ACATCTC	11175	0.0	21.971346	8
ACACATC	11290	0.0	21.74947	6
TACACAT	12875	0.0	20.864122	5
GCAGAGT	29660	0.0	19.77714	9
TACATGG	21740	0.0	19.760366	2
GTACATG	22415	0.0	19.632755	1
>>END_MODULE