FastQCFastQC Report
Fri 27 May 2016
SRR2138935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6788180
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83200.048.1981281
CTTATAC83850.045.5662571
TATACAC117300.035.117443
GTATCAA147100.034.440891
TTATACA118750.033.1313062
TCAACGC170350.028.8248394
ATCAACG171150.028.6901053
CAACGCA173800.028.2768655
TATCAAC181600.027.4112912
AACGCAG181950.027.030216
ACATCTC147700.026.0872548
TACACAT166150.024.5645945
ACACATC157500.024.2544966
ACGCAGA208300.023.647277
CGCAGAG210500.023.3401788
CACATCT163150.023.2558827
CATCTCC164150.023.1910889
ATACACA200900.022.158684
GTGGTAT45350.020.7841031
GCAGAGT251050.019.5534789
Produced by FastQC (version 0.11.5)