##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6788180
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42460571169297	34.0	31.0	34.0	31.0	34.0
2	32.55619105562905	34.0	31.0	34.0	31.0	34.0
3	32.6058688779614	34.0	31.0	34.0	31.0	34.0
4	36.13589533571591	37.0	35.0	37.0	35.0	37.0
5	36.043141607912574	37.0	35.0	37.0	35.0	37.0
6	36.06311839108568	37.0	35.0	37.0	35.0	37.0
7	36.016293910886276	37.0	35.0	37.0	35.0	37.0
8	36.01214758595087	37.0	35.0	37.0	35.0	37.0
9	37.736716174291196	39.0	38.0	39.0	35.0	39.0
10-11	37.66588017701358	39.0	37.0	39.0	35.0	39.0
12-13	37.64459693172544	39.0	37.0	39.0	35.0	39.0
14-15	39.068329213426864	41.0	38.0	41.0	35.5	41.0
16-17	39.04505950048467	41.0	38.0	41.0	35.0	41.0
18-19	39.043614488714205	41.0	38.5	41.0	35.0	41.0
20-21	39.03561163581181	41.0	39.0	41.0	35.0	41.0
22-23	39.025009835195455	41.0	39.0	41.0	35.0	41.0
24-25	38.98470264332623	41.0	39.0	41.0	35.0	41.0
26-27	38.93019563619639	41.0	38.0	41.0	35.0	41.0
28-29	38.86832282674901	40.0	38.0	41.0	35.0	41.0
30-31	38.78887628918248	40.0	38.0	41.0	34.5	41.0
32-33	38.68320278987681	40.0	38.0	41.0	34.0	41.0
34-35	38.58723964809103	40.0	38.0	41.0	34.0	41.0
36-37	38.55184848108097	40.0	38.0	41.0	34.0	41.0
38-39	38.46214427877186	40.0	38.0	41.0	34.0	41.0
40-41	38.478407457199864	40.0	38.0	41.0	34.0	41.0
42-43	38.42908457253478	40.0	38.0	41.0	33.5	41.0
44-45	38.344379061827524	40.0	38.0	41.0	33.0	41.0
46-47	38.28787513840301	40.0	38.0	41.0	33.0	41.0
48-49	38.24720040683795	40.0	38.0	41.0	33.0	41.0
50-51	38.15693767403277	40.0	37.5	41.0	33.0	41.0
52-53	38.05054392223917	40.0	37.0	41.0	33.0	41.0
54-55	37.9313564239926	40.0	37.0	41.0	33.0	41.0
56-57	37.81929493486147	40.0	37.0	41.0	32.0	41.0
58-59	37.73257173937341	40.0	36.0	41.0	32.0	41.0
60-61	37.5927276068984	39.5	36.0	41.0	32.0	41.0
62-63	37.427869823288596	39.0	35.5	41.0	32.0	41.0
64-65	37.244287962323426	39.0	35.0	41.0	32.0	41.0
66-67	37.029167032794675	39.0	35.0	41.0	32.0	41.0
68-69	36.71694083434321	38.0	35.0	40.5	31.0	41.0
70-71	36.41011439289412	37.0	35.0	40.0	31.0	41.0
72-73	36.1166445111201	37.0	35.0	39.5	31.0	41.0
74-75	35.858686481836514	36.0	35.0	39.0	31.0	41.0
76-77	35.572193078642215	36.0	35.0	39.0	31.0	40.5
78-79	35.295498088812714	35.5	34.0	37.5	31.0	39.5
80-81	35.01389744131908	35.0	34.0	37.0	31.0	39.0
82-83	34.711524848639684	35.0	34.0	37.0	31.0	39.0
84-85	34.46749039568435	35.0	34.0	36.0	31.0	38.0
86-87	34.293583008266424	35.0	34.0	36.0	31.0	37.0
88-89	34.14078620122032	35.0	34.0	36.0	31.0	37.0
90-91	33.995770446518776	35.0	34.0	35.0	31.0	36.5
92-93	33.93104055065096	35.0	34.0	35.0	31.0	36.0
94-95	33.87258816522875	35.0	34.0	35.0	31.0	36.0
96-97	33.86051977270357	35.0	34.0	35.0	31.0	36.0
98-99	33.9918249333941	35.0	34.0	35.0	31.0	36.0
100	31.24268557100313	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	12.0
19	39.0
20	78.0
21	268.0
22	1037.0
23	3025.0
24	7928.0
25	16235.0
26	29189.0
27	46488.0
28	67826.0
29	92783.0
30	120204.0
31	151066.0
32	187539.0
33	236230.0
34	307104.0
35	421784.0
36	616149.0
37	1125838.0
38	1835081.0
39	1398188.0
40	124086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.68312566844132	21.119180693499583	14.748842841527479	24.44885079653162
2	14.40697967355706	20.910837381090236	42.27225382345445	22.40992912189825
3	18.189367412626293	26.094297298726655	30.62999757371981	25.086337714927247
4	12.574681284232298	18.595529287673575	38.12665545109293	30.7031339770012
5	12.847102822023992	37.885342807361525	36.07420693467511	13.193347435939367
6	29.54771744495274	38.310946098256316	18.569353282208816	13.571983174582128
7	25.936952172747336	32.6269928021944	22.952411397458526	18.48364362759974
8	23.471608000966384	38.12887990595417	21.16714642216323	17.23236567091621
9	24.534028266781377	17.479221234557716	21.48605369922424	36.50069679943667
10-11	23.91766036136509	27.204507720151355	28.885305206781233	19.992526711702325
12-13	24.64317931533474	24.30772850183595	29.16555597352073	21.88353620930858
14-15	21.66485297456063	25.951886360097458	26.482492580116112	25.9007680852258
16-17	21.4567696739162	29.3564768553731	27.22586360993394	21.960889860776767
18-19	21.53007504369956	28.185770077583435	29.009194972710745	21.27495990600626
20-21	22.045479289070833	27.252377908401154	29.05387656930611	21.648266233221904
22-23	21.768264745737063	27.67198924203196	28.82205539634497	21.737690615886006
24-25	21.789798996559796	27.765009525342304	28.99809404724322	21.44709743085468
26-27	21.820241435697508	27.92649732450792	28.94933300697589	21.30392823281868
28-29	21.522151034614087	27.63998556556619	29.175396447066376	21.66246695275335
30-31	21.808651587968868	27.950046191400435	28.83125928945197	21.41004293117873
32-33	22.15960062824821	27.479796964336543	28.915321743018975	21.44528066439627
34-35	21.82560638688444	27.534052454853086	29.01921908591674	21.621122072345734
36-37	21.60029212255989	27.915812598951735	28.883034940465972	21.600860338022407
38-39	21.57987938202207	27.468065454663204	29.18046872852596	21.771586434788766
40-41	21.784600289886036	27.41367605356822	29.115838484435013	21.68588517211073
42-43	21.941642195196394	27.404792167335312	28.94685414432836	21.706711493139935
44-45	21.762300849560408	27.743765631442376	28.679615302950623	21.814318216046594
46-47	22.03637570736779	27.48124054543136	28.24319575100654	22.239187996194318
48-49	21.986377214364133	27.527876564943842	28.27082377550768	22.214922445184342
50-51	21.699580692494	27.70283577384958	28.43833664993029	22.159246883726137
52-53	21.800442843506616	27.746152204056752	28.604185537838823	21.84921941459781
54-55	21.842761488838736	27.737135217841345	28.299729190547417	22.120374102772498
56-57	22.058928902884915	28.03186946490468	27.930918081261787	21.978283550948614
58-59	21.67819627446258	28.37489454225746	28.36877437373566	21.578134809544306
60-61	21.99940321576042	28.674494713544398	27.747627683017566	21.57847438767762
62-63	21.69668977580637	28.976378863400416	27.676569370495237	21.650361990297984
64-65	21.654051784914785	28.862223147567455	27.77463498939097	21.709090078126785
66-67	21.772921293101824	28.656765738725763	27.762334925083852	21.807978043088553
68-69	21.793557134615877	28.38757300689034	27.901659111019868	21.917210747473913
70-71	21.840654434785858	28.046124514285857	28.023335514536917	22.089885536391364
72-73	21.963188551098177	27.915579432079817	28.028267359894244	22.092964656927766
74-75	21.831437096039842	28.04047240613831	28.04161870997116	22.086471787850684
76-77	21.931113976170575	28.03678295113	27.93187945881661	22.100223613882815
78-79	21.89686445609346	27.996769987075016	27.96735973478894	22.13900582204258
80-81	21.91887840381476	28.037707869694394	27.998784581799868	22.044629144690976
82-83	21.904015339099423	28.01638267456331	28.09509853614055	21.98450345019671
84-85	22.038813767714007	28.00173800023469	27.992256285288402	21.967191946762902
86-87	21.871297975028366	28.0168344814781	28.083397694285367	22.02846984920817
88-89	21.938198909412925	28.00936105066268	28.04882479475721	22.003615245167186
90-91	21.90305868686919	28.10756307506692	28.029673406923557	21.959704831140332
92-93	21.964721866786384	28.05598460561784	28.074199181629584	21.905094345966194
94-95	21.819410537816808	28.16447255553306	28.086312334307305	21.929804572342825
96-97	21.85310769016695	28.282895346466287	28.167851769773698	21.696145193593065
98-99	22.87570557154493	30.095009390118744	28.45188663131068	18.577398407025647
100	22.029131594728423	31.21762102446221	21.82223957530478	24.931007805504592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1373.0
1	1111.5
2	1309.0
3	1814.0
4	2321.5
5	3948.5
6	5466.0
7	6650.0
8	7469.5
9	7222.0
10	6069.5
11	4755.5
12	4868.0
13	6815.5
14	12433.0
15	20848.5
16	27944.5
17	32195.5
18	33599.0
19	32983.0
20	31254.5
21	29114.0
22	28683.0
23	31667.0
24	38327.5
25	48523.0
26	61889.0
27	76487.5
28	91515.0
29	108875.5
30	125326.5
31	140464.0
32	156599.5
33	170705.5
34	184752.5
35	199396.5
36	214543.5
37	229471.5
38	239908.5
39	247498.0
40	255552.0
41	262749.0
42	268087.0
43	275877.5
44	290579.0
45	307556.5
46	308391.0
47	298853.5
48	289543.5
49	279711.5
50	273770.0
51	266371.5
52	254427.5
53	238671.5
54	221104.0
55	203821.5
56	186575.0
57	168454.5
58	149377.0
59	129284.0
60	108399.0
61	88455.0
62	71184.0
63	56027.0
64	42772.0
65	32302.5
66	24408.5
67	18821.0
68	14823.5
69	11387.0
70	8697.0
71	6698.5
72	5099.5
73	3791.5
74	2845.0
75	2171.0
76	1649.5
77	1236.5
78	926.0
79	704.0
80	523.5
81	371.0
82	280.5
83	193.0
84	134.5
85	101.5
86	80.0
87	59.0
88	39.0
89	27.5
90	18.5
91	15.5
92	12.0
93	7.5
94	3.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006923799899236614
3	1.620463806204314E-4
4	0.0
5	0.011520024513197941
6	0.012227135992268915
7	0.0
8	0.0
9	0.0
10-11	5.1560212015591804E-5
12-13	2.209723372096792E-5
14-15	1.4731489147311946E-5
16-17	2.5043531550430305E-4
18-19	0.0010091070065908682
20-21	0.0
22-23	1.178760243334424E-4
24-25	0.0
26-27	1.4745063021136605E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.2065672068529724E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.822869337154979E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.005801522446646656
52-53	5.609262462919771E-5
54-55	9.695707492139812E-5
56-57	0.0
58-59	1.6452063212117274E-5
60-61	1.660498744081775E-4
62-63	9.469953304749718E-4
64-65	0.0012102692104212134
66-67	0.0010786575952133628
68-69	0.009055037122186157
70-71	0.0011583030894839154
72-73	8.162510617918024E-4
74-75	5.194162263121574E-4
76-77	9.946020235630261E-5
78-79	0.0
80-81	2.307517078395401E-4
82-83	0.0
84-85	0.0
86-87	3.0485420300108005E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	212.0
20-21	847.0
22-23	1525.0
24-25	2718.0
26-27	4428.0
28-29	7586.0
30-31	18781.0
32-33	22725.0
34-35	31306.0
36-37	45851.0
38-39	49914.0
40-41	49333.0
42-43	56529.0
44-45	66037.0
46-47	65550.0
48-49	59686.0
50-51	52893.0
52-53	50694.0
54-55	54040.0
56-57	55530.0
58-59	55420.0
60-61	56031.0
62-63	57144.0
64-65	65403.0
66-67	70523.0
68-69	72349.0
70-71	66303.0
72-73	52687.0
74-75	52639.0
76-77	54943.0
78-79	57085.0
80-81	57231.0
82-83	55461.0
84-85	55856.0
86-87	59063.0
88-89	63308.0
90-91	66806.0
92-93	71652.0
94-95	91649.0
96-97	235479.0
98-99	536089.0
100-101	4138874.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.57629010788682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.77926056708974	75.08620497285857
2	7.8634749290097075	13.301270737040394
3	1.9488163099969116	4.9447096080384085
4	0.693825238666189	2.347246586784216
5	0.30761691662979124	1.3008548791487429
6	0.1528317304897911	0.7755564465356966
7	0.08222216560968232	0.4867832012332244
8	0.05194847764675963	0.3514887612892333
9	0.03098891778073337	0.23588349303174816
>10	0.08776861573754047	1.074923076561418
>50	9.676021014220182E-4	0.0544360035135312
>100	2.7852924172968023E-4	0.04064223396471155
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4731489147311944E-5
2	0.0	1.4731489147311944E-5	0.0	0.0	1.4731489147311944E-5
3	0.0	1.4731489147311944E-5	0.0	0.0	1.4731489147311944E-5
4	0.0	1.4731489147311944E-5	0.0	0.0	1.4731489147311944E-5
5	0.0	5.892595658924778E-5	0.0	1.4731489147311944E-5	1.4731489147311944E-5
6	0.0	5.892595658924778E-5	0.0	1.4731489147311944E-5	1.4731489147311944E-5
7	0.0	7.365744573655973E-5	0.0	1.4731489147311944E-5	1.4731489147311944E-5
8	0.0	7.365744573655973E-5	0.0	2.946297829462389E-5	1.4731489147311944E-5
9	0.0	1.0312042403118362E-4	0.0	7.365744573655973E-5	1.4731489147311944E-5
10-11	0.0	1.1785191317849556E-4	0.0	8.102319031021569E-5	1.4731489147311944E-5
12-13	0.0	1.1785191317849556E-4	0.0	1.1785191317849556E-4	1.4731489147311944E-5
14-15	0.0	1.3258340232580752E-4	0.0	1.1785191317849556E-4	1.4731489147311944E-5
16-17	0.0	1.3258340232580752E-4	0.0	1.3258340232580752E-4	1.4731489147311944E-5
18-19	0.0	1.3258340232580752E-4	0.0	1.3994914689946348E-4	1.4731489147311944E-5
20-21	0.0	1.5468063604677542E-4	0.0	1.6204638062043139E-4	1.4731489147311944E-5
22-23	0.0	1.6941212519408738E-4	0.0	1.6941212519408738E-4	1.4731489147311944E-5
24-25	0.0	1.7677786976774335E-4	0.0	2.1360659263602318E-4	1.4731489147311944E-5
26-27	0.0	1.7677786976774335E-4	0.0	3.3145850581451877E-4	1.4731489147311944E-5
28-29	0.0	1.7677786976774335E-4	0.0	8.175976476758129E-4	1.4731489147311944E-5
30-31	0.0	1.9150935891505528E-4	0.0	0.002747422725973678	1.4731489147311944E-5
32-33	0.0	2.0624084806236724E-4	0.0	0.0066954618174532785	1.4731489147311944E-5
34-35	0.0	2.5043531550430305E-4	0.0	0.0119619691876173	1.4731489147311944E-5
36-37	0.0	3.0199552751989484E-4	0.0	0.020093751196933494	1.4731489147311944E-5
38-39	0.0	3.0936127209355084E-4	0.0	0.03168743315586799	3.682872286827986E-5
40-41	0.0	3.2409276124086277E-4	0.0	0.04220571640704872	4.419446744193584E-5
42-43	0.0	3.3145850581451877E-4	0.0	0.051103535852025136	4.419446744193584E-5
44-45	0.0	3.830187178301106E-4	0.0	0.060833684433824683	4.419446744193584E-5
46-47	0.0	4.272131852720464E-4	0.0	0.0699819391943054	5.156021201559181E-5
48-49	0.0	4.4194467441935835E-4	0.0	0.07832732779625762	5.892595658924778E-5
50-51	0.0	4.566761635666703E-4	0.0	0.08523639620634692	5.892595658924778E-5
52-53	0.0	4.566761635666703E-4	0.0	0.0926684324811658	7.365744573655973E-5
54-55	0.0	4.935048864349502E-4	0.0	0.09923867664086691	8.838893488387167E-5
56-57	0.0	5.303336093032301E-4	0.0	0.10485137400599276	8.838893488387167E-5
58-59	0.0	5.745280767451658E-4	0.0	0.11069977519747562	8.838893488387167E-5
60-61	0.0	5.892595658924778E-4	0.0	0.11608413448081814	8.838893488387167E-5
62-63	0.0	6.039910550397898E-4	0.0	0.1209234286657101	1.0312042403118362E-4
64-65	0.0	6.260882887607576E-4	0.0	0.12576272285060208	1.0312042403118362E-4
66-67	0.0	6.629170116290375E-4	0.0	0.1310144987316188	1.0312042403118362E-4
68-69	0.0	6.776485007763495E-4	0.0	0.1358979873839527	1.0312042403118362E-4
70-71	0.0	7.144772236446293E-4	0.0	0.14067835561225542	1.0312042403118362E-4
72-73	0.0	7.365744573655973E-4	0.0	0.1441623527955947	1.3258340232580752E-4
74-75	0.0	7.439402019392533E-4	0.0	0.1469024097769947	1.3258340232580752E-4
76-77	0.0	7.586716910865652E-4	0.0	0.14948042037777431	1.3258340232580752E-4
78-79	0.0	7.660374356602211E-4	0.0	0.15124083333087807	1.3258340232580752E-4
80-81	0.0	7.660374356602211E-4	0.0	0.1517269724727394	1.4731489147311945E-4
82-83	0.0	7.660374356602211E-4	0.0	0.15224994033746897	1.7677786976774335E-4
84-85	0.0	7.734031802338771E-4	0.0	0.15241935246266303	1.7677786976774335E-4
86-87	0.0	8.10231903102157E-4	0.0	0.15250037565297325	1.8414361434139931E-4
88	0.0	8.24963392249469E-4	0.0	0.15251510714212058	1.9150935891505528E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8320	0.0	48.198128	1
CTTATAC	8385	0.0	45.566257	1
TATACAC	11730	0.0	35.11744	3
GTATCAA	14710	0.0	34.44089	1
TTATACA	11875	0.0	33.131306	2
TCAACGC	17035	0.0	28.824839	4
ATCAACG	17115	0.0	28.690105	3
CAACGCA	17380	0.0	28.276865	5
TATCAAC	18160	0.0	27.411291	2
AACGCAG	18195	0.0	27.03021	6
ACATCTC	14770	0.0	26.087254	8
TACACAT	16615	0.0	24.564594	5
ACACATC	15750	0.0	24.254496	6
ACGCAGA	20830	0.0	23.64727	7
CGCAGAG	21050	0.0	23.340178	8
CACATCT	16315	0.0	23.255882	7
CATCTCC	16415	0.0	23.191088	9
ATACACA	20090	0.0	22.15868	4
GTGGTAT	4535	0.0	20.784103	1
GCAGAGT	25105	0.0	19.553478	9
>>END_MODULE