FastQCFastQC Report
Fri 27 May 2016
SRR2138934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9258583
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129800.052.288191
GTATCAA240200.039.2529261
TCAACGC294750.031.3297354
ATCAACG297800.030.9950643
TATCAAC302250.030.8238682
CAACGCA300250.030.7233145
AACGCAG307950.029.9669956
ACGCAGA354700.026.078037
CGCAGAG369350.025.0203348
GTACATG316250.022.9869981
GCAGAGT414550.022.2299149
TACATGG331500.021.6565482
ACATGGG337350.020.9488833
GAGTACT228600.020.45773312-13
CAGAGTA404950.019.58647510-11
CATGGGG259700.019.3802434
AGAGTAC373950.019.23431810-11
GTACTTT256250.018.23392514-15
GTGGTAT61650.018.1035791
AGTACTT256550.017.12900212-13
Produced by FastQC (version 0.11.5)