##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138934_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9258583
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72373310257088	33.0	33.0	33.0	33.0	33.0
2	32.71448751931046	33.0	33.0	33.0	33.0	33.0
3	32.71196629117004	33.0	33.0	33.0	33.0	33.0
4	36.640479974095385	37.0	37.0	37.0	37.0	37.0
5	36.65747533936889	37.0	37.0	37.0	37.0	37.0
6	36.57591447848985	37.0	37.0	37.0	37.0	37.0
7	36.628882735079436	37.0	37.0	37.0	37.0	37.0
8	36.632812170069656	37.0	37.0	37.0	37.0	37.0
9	36.642544760899156	37.0	37.0	37.0	37.0	37.0
10-11	36.6022483678118	37.0	37.0	37.0	37.0	37.0
12-13	36.57276675059239	37.0	37.0	37.0	37.0	37.0
14-15	38.675853583642336	40.0	37.0	40.0	37.0	40.0
16-17	38.62462997847511	40.0	37.0	40.0	37.0	40.0
18-19	38.65137553986393	40.0	37.0	40.0	37.0	40.0
20-21	38.67252020262288	40.0	37.0	40.0	37.0	40.0
22-23	38.511274938838035	40.0	37.0	40.0	37.0	40.0
24-25	38.67045938532098	40.0	37.0	40.0	37.0	40.0
26-27	38.45400070502331	40.0	37.0	40.0	37.0	40.0
28-29	38.45064749036932	40.0	37.0	40.0	37.0	40.0
30-31	38.43386571063791	40.0	37.0	40.0	37.0	40.0
32-33	38.42810976553232	40.0	37.0	40.0	37.0	40.0
34-35	38.33387322925408	40.0	37.0	40.0	37.0	40.0
36-37	33.05086723890551	40.0	33.0	40.0	8.5	40.0
38-39	37.187433431719214	40.0	35.0	40.0	33.0	40.0
40-41	37.795479414383436	40.0	37.0	40.0	33.0	40.0
42-43	38.01344377294476	40.0	37.0	40.0	33.0	40.0
44-45	38.01708597727999	40.0	37.0	40.0	33.0	40.0
46-47	37.901577762435274	40.0	37.0	40.0	33.0	40.0
48-49	37.87043990003066	40.0	37.0	40.0	33.0	40.0
50-51	37.808357300813874	40.0	37.0	40.0	33.0	40.0
52-53	37.7199286512804	40.0	37.0	40.0	33.0	40.0
54-55	37.61575327534466	40.0	37.0	40.0	33.0	40.0
56-57	37.51757252337264	40.0	37.0	40.0	33.0	40.0
58-59	37.35024144117318	37.0	37.0	40.0	33.0	40.0
60-61	37.13620674362119	37.0	37.0	40.0	33.0	40.0
62-63	36.89263336187423	37.0	37.0	40.0	33.0	40.0
64-65	36.66526190172743	37.0	37.0	40.0	33.0	40.0
66-67	36.38148202466975	37.0	37.0	40.0	33.0	40.0
68-69	36.10121860234169	37.0	37.0	38.5	33.0	40.0
70-71	35.83422953880676	37.0	37.0	37.0	33.0	40.0
72-73	35.58356100157782	37.0	33.0	37.0	33.0	40.0
74-75	35.303075706122954	37.0	33.0	37.0	33.0	40.0
76-77	33.43947937306898	35.0	33.0	37.0	27.0	37.0
78-79	34.96678536887451	37.0	33.0	37.0	33.0	37.0
80-81	35.026372108686004	37.0	33.0	37.0	33.0	37.0
82-83	35.124889367884585	37.0	33.0	37.0	33.0	37.0
84-85	34.98403571388452	37.0	33.0	37.0	33.0	37.0
86-87	34.92810629617635	37.0	33.0	37.0	33.0	37.0
88-89	34.84510966380985	37.0	33.0	37.0	33.0	37.0
90-91	34.70443248131194	37.0	33.0	37.0	33.0	37.0
92-93	34.68711802537811	37.0	33.0	37.0	33.0	37.0
94-95	34.65361018843366	37.0	33.0	37.0	33.0	37.0
96-97	34.633852189063234	37.0	33.0	37.0	33.0	37.0
98-99	34.59331065027534	37.0	33.0	37.0	33.0	37.0
100	34.5431880178969	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	58.0
21	277.0
22	1061.0
23	3258.0
24	7665.0
25	15566.0
26	27820.0
27	45180.0
28	66339.0
29	94182.0
30	127672.0
31	171656.0
32	228209.0
33	309040.0
34	438581.0
35	697985.0
36	1382819.0
37	3027169.0
38	2541176.0
39	72866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.26972961197194	19.812189403065243	14.037191220297965	23.880889764664854
2	15.01996579822204	20.867523680459527	40.968266958345566	23.144243562972868
3	18.187207880235935	26.478648639511587	30.024119710494347	25.310023769758132
4	12.892592604595041	17.912560478840103	38.1136651122295	31.081181804335355
5	13.773009276081932	37.54491893743948	34.91310235231137	13.76896943416721
6	30.281707254771064	37.381314181662574	18.525739845935387	13.81123871763098
7	26.923633994532427	32.37818357301544	22.974573970984544	17.72360846146759
8	25.176379582059155	35.78120971643285	20.782165046206313	18.26024565530168
9	26.237611090163583	16.520681404487057	20.817980462021023	36.423727043328334
10-11	24.79558697048998	26.28190512522273	28.32309220536231	20.599415698924986
12-13	25.747741312034467	23.99534032367588	28.330388138228063	21.92653022606159
14-15	22.93497827907359	25.015885260195862	25.985850102548092	26.06328635818246
16-17	22.40468654868677	28.152747564071092	26.57796014789736	22.86460573934478
18-19	22.536699190362068	27.290623197955888	28.350645017709514	21.822032593972533
20-21	23.30335543691945	26.411654008040504	28.264532757739286	22.020457797300757
22-23	23.275359134665603	26.34220947468923	28.036608074606555	22.34582331603861
24-25	22.940554885315485	26.570519777027744	28.113795708593326	22.375129629063444
26-27	22.777437107066962	26.60208306702012	28.119939992708538	22.50053983320438
28-29	22.776593016218758	26.53114239872209	28.197302263578305	22.494962321480845
30-31	22.893288225340164	26.550967129239964	28.041748371263157	22.51399627415671
32-33	22.776965052789375	26.721899092014866	27.950751195773233	22.55038465942253
34-35	23.002586144957395	26.62019355061379	27.937371400935607	22.439848903493207
36-37	23.164804086579736	26.57512346036671	27.66947751976387	22.590594933289687
38-39	22.84726485332055	26.70446386011807	27.909533229659594	22.53873805690178
40-41	23.00874497689923	26.529554627301223	27.84728119080751	22.61441920499204
42-43	22.83832029701916	26.702038250556225	27.60513526009512	22.854506192329495
44-45	22.85701157926262	26.77104259864406	27.485195848803627	22.886749973289696
46-47	23.10045813935274	26.737198813860296	27.166768912080002	22.99557413470696
48-49	23.037313541286583	26.919574601244072	27.00164059035667	23.041471267112676
50-51	23.00728494875627	27.01412809304744	26.91931140352684	23.05927555466945
52-53	23.23854788458114	26.92638480741029	26.81573404801898	23.019333259989587
54-55	23.183651428205735	26.83013088150878	26.832712393313894	23.15350529697159
56-57	23.13194962712225	26.998086560448005	26.8310158741219	23.03894793830785
58-59	23.05128414865378	27.015620900938043	26.840283728367975	23.0928112220402
60-61	23.155026472673907	27.110019301631866	26.719380955626548	23.01557327006768
62-63	23.18228435964065	27.120991274068235	26.767171647353543	22.929552718937572
64-65	23.166530458473545	26.98497360617289	26.699914983753466	23.1485809516001
66-67	23.19420366186346	26.98536445815838	26.613747848942065	23.206684031036097
68-69	23.06614371679524	27.008617242323762	26.69632923481921	23.228909806061786
70-71	23.11493291929454	26.905898477690805	26.71773054284396	23.261438060170693
72-73	23.19961047866203	26.96007157866597	26.535662487710066	23.304655454961928
74-75	23.051070963740653	27.119767705088694	26.571023556017686	23.25813777515297
76-77	23.27887560622296	26.97342094084721	26.556620350978054	23.191083101951776
78-79	23.1365428483828	26.931661227539415	26.513450801283167	23.418345122794623
80-81	23.23809789511295	27.04176470823729	26.437257731814313	23.282879664835445
82-83	23.268820518490525	26.998599759028295	26.574135148623196	23.158444573857977
84-85	23.173797558607465	27.076248854791185	26.49869739960774	23.25125618699361
86-87	23.19116910343168	27.04329665619869	26.621742929422066	23.143791310947567
88-89	23.21536638498474	26.96112098897568	26.59678905837339	23.22672356766619
90-91	23.220914165728455	27.062170494855042	26.54819643553931	23.168718903877192
92-93	23.279579257141744	27.074314712061426	26.56119584917586	23.08491018162097
94-95	23.071032214918404	26.973012578390748	26.708743818535858	23.247211388154994
96-97	23.243894018638343	27.07747992116109	26.68856819340671	22.990057866793855
98-99	24.401793518557085	29.003017883997106	26.77517229909888	19.820016298346925
100	23.29523447029383	29.907875491015094	20.71807752047389	26.078812518217187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2503.0
1	2234.0
2	2838.0
3	3904.0
4	5009.0
5	8055.5
6	10845.5
7	12893.5
8	14356.5
9	14065.0
10	12430.5
11	10719.5
12	11345.5
13	15118.0
14	23968.0
15	36071.0
16	46595.0
17	53283.0
18	54326.0
19	50036.5
20	43409.0
21	37007.0
22	32713.0
23	32255.5
24	35577.5
25	43063.0
26	53712.0
27	65549.5
28	80159.0
29	97895.0
30	116182.0
31	134842.0
32	156247.5
33	177799.0
34	196661.0
35	213997.0
36	234375.0
37	254634.0
38	272000.5
39	287135.0
40	297823.5
41	307148.5
42	314148.0
43	322722.5
44	334516.5
45	347669.0
46	358797.0
47	367040.5
48	372712.5
49	375747.5
50	378396.5
51	377344.5
52	370971.0
53	357956.5
54	342067.5
55	324762.0
56	304334.0
57	280026.0
58	252976.5
59	221776.5
60	186751.0
61	153874.5
62	124532.5
63	98502.5
64	75286.0
65	56601.0
66	43405.5
67	33129.0
68	24397.5
69	17895.5
70	13228.5
71	9521.5
72	6496.5
73	4381.0
74	3052.0
75	2091.5
76	1339.5
77	795.0
78	519.5
79	369.5
80	256.5
81	166.0
82	111.5
83	70.5
84	48.0
85	40.0
86	35.0
87	26.5
88	15.0
89	6.5
90	3.0
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0019441419923545537
4	0.013101356870700409
5	0.008586627132899278
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0018041598524521343
26-27	0.03561765206946285
28-29	0.08083175519340244
30-31	0.07795656957338482
32-33	0.03527998920962099
34-35	0.0014760387894311282
36-37	7.7715284879243525
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.1397401814090253E-5
56-57	2.292092852681462E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011286652996669982
74-75	0.03324006174043865
76-77	0.0041282737718585484
78-79	0.0
80-81	0.0
82-83	0.0034077174956759853
84-85	0.03856794876128954
86-87	0.04138299781365932
88-89	0.01184900474524737
90-91	6.561314698499716E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	113.0
20-21	516.0
22-23	989.0
24-25	2365.0
26-27	3328.0
28-29	5660.0
30-31	11651.0
32-33	15305.0
34-35	24455.0
36-37	26275.0
38-39	32318.0
40-41	39233.0
42-43	49211.0
44-45	62150.0
46-47	60355.0
48-49	52551.0
50-51	44744.0
52-53	42083.0
54-55	46823.0
56-57	51311.0
58-59	53661.0
60-61	54701.0
62-63	56970.0
64-65	60026.0
66-67	64785.0
68-69	63921.0
70-71	64475.0
72-73	62350.0
74-75	64082.0
76-77	62557.0
78-79	68814.0
80-81	70291.0
82-83	69326.0
84-85	70469.0
86-87	75349.0
88-89	82692.0
90-91	89777.0
92-93	96387.0
94-95	119152.0
96-97	338569.0
98-99	593440.0
100-101	6405353.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.99661401710493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.8259684281977	73.72254616330673
2	7.453937371688254	12.373031258913672
3	1.9996643607170146	4.978961133305727
4	0.7622107806705534	2.5304365585196056
5	0.3548906134474189	1.4727359631294508
6	0.1969091376761668	0.9805675017689873
7	0.11129156905628464	0.6465776380225705
8	0.07820023889478174	0.5192284034876476
9	0.050820511184498006	0.37961373158385703
>10	0.16208905821278216	2.09569969136948
>50	0.003186330277518455	0.18448816967580203
>100	7.8357425960742E-4	0.0926762928633461
>500	4.802571741414091E-5	0.023437494053100666
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1601577692828374E-5	0.0	0.0	0.0
3	0.0	2.1601577692828374E-5	0.0	0.0	0.0
4	0.0	1.620118326962128E-4	0.0	0.0	0.0
5	0.0	1.72812621542627E-4	0.0	0.0	0.0
6	7.56055219248993E-5	1.72812621542627E-4	0.0	0.0	0.0
7	7.56055219248993E-5	1.72812621542627E-4	0.0	0.0	0.0
8	7.56055219248993E-5	2.0521498808186956E-4	0.0	0.0	0.0
9	7.56055219248993E-5	4.104299761637391E-4	0.0	0.0	0.0
10-11	8.10059163481064E-5	4.104299761637391E-4	0.0	1.0800788846414187E-5	0.0
12-13	8.64063107713135E-5	4.158303705869462E-4	0.0	1.0800788846414187E-5	0.0
14-15	9.720709961772767E-5	6.048441753991945E-4	0.0	1.0800788846414187E-5	0.0
16-17	1.620118326962128E-4	6.156449642456086E-4	0.0	1.0800788846414187E-5	0.0
18-19	1.620118326962128E-4	6.642485140544725E-4	0.0	1.620118326962128E-5	0.0
20-21	1.620118326962128E-4	7.128520638633363E-4	0.0	3.240236653924256E-5	0.0
22-23	1.620118326962128E-4	8.262603467506853E-4	0.0	4.860354980886384E-5	0.0
24-25	1.620118326962128E-4	8.802642909827563E-4	0.0	6.480473307848512E-5	0.0
26-27	1.620118326962128E-4	8.964654742523775E-4	1.0800788846414187E-5	7.560552192489931E-5	0.0
28-29	1.620118326962128E-4	8.964654742523775E-4	1.0800788846414187E-5	2.2681656577469792E-4	0.0
30-31	1.620118326962128E-4	9.82871785023691E-4	1.0800788846414187E-5	8.046587690578569E-4	0.0
32-33	1.620118326962128E-4	9.82871785023691E-4	1.0800788846414187E-5	0.002138556191590009	0.0
34-35	1.620118326962128E-4	0.0010692780957950046	1.0800788846414187E-5	0.004109700156060598	0.0
36-37	1.620118326962128E-4	0.0011826863786823533	1.0800788846414187E-5	0.0078089703359574564	0.0
38-39	1.620118326962128E-4	0.0012474911117608387	1.0800788846414187E-5	0.019252406118733287	0.0
40-41	1.620118326962128E-4	0.0013068954504161165	1.0800788846414187E-5	0.0305284296743897	0.0
42-43	1.72812621542627E-4	0.001328497028108945	1.0800788846414187E-5	0.03841300553227205	0.0
44-45	1.8361341038904117E-4	0.0014689072831123294	1.0800788846414187E-5	0.048468539948283665	0.0
46-47	1.9441419923545535E-4	0.0015877159604228855	1.0800788846414187E-5	0.05916132090623371	0.0
48-49	1.9441419923545535E-4	0.0015985167492692997	1.0800788846414187E-5	0.06960028332629302	0.0
50-51	2.4301774904431919E-4	0.0018361341038904116	1.0800788846414187E-5	0.08025526152328061	0.0
52-53	2.592189323139405E-4	0.00185773568158324	1.0800788846414187E-5	0.1062743618542924	0.0
54-55	2.6461932673714755E-4	0.0018847376536992755	1.0800788846414187E-5	0.13925457059681812	0.0
56-57	2.7001972116035466E-4	0.0019225404146617252	1.0800788846414187E-5	0.1732770554630228	0.0
58-59	2.7001972116035466E-4	0.0019279408090849322	1.0800788846414187E-5	0.19903153646729743	0.0
60-61	2.9702169327639014E-4	0.0019495423867777607	1.0800788846414187E-5	0.22053590706050807	0.0
62-63	3.5642603193166816E-4	0.001971143964470589	1.0800788846414187E-5	0.23766595817092098	0.0
64-65	3.5642603193166816E-4	0.0023437711796718786	1.0800788846414187E-5	0.24177025793255835	0.0
66-67	3.7262721520128946E-4	0.002376173546211121	1.0800788846414187E-5	0.24569634467822993	0.0
68-69	3.780276096244965E-4	0.002419376701596778	1.0800788846414187E-5	0.24996805666698674	0.0
70-71	3.834280040477036E-4	0.0024571794625592273	1.0800788846414187E-5	0.2540507548509313	0.0
72-73	4.212307650101533E-4	0.0024733806458288486	1.0800788846414187E-5	0.2574962064929374	0.0
74-75	4.212307650101533E-4	0.0025381853789073336	1.0800788846414187E-5	0.2601586009435785	0.0
76-77	4.266311594333604E-4	0.002673195239487511	1.0800788846414187E-5	0.2625239737009432	0.0
78-79	4.3203155385656747E-4	0.002721798789296375	1.0800788846414187E-5	0.2640954884780965	0.0
80-81	4.3203155385656747E-4	0.002721798789296375	1.0800788846414187E-5	0.26454912160964583	0.0
82-83	4.4283234270298165E-4	0.002737999972565996	1.0800788846414187E-5	0.2650459578965809	0.0
84-85	4.5363313154939583E-4	0.002775802733528446	1.0800788846414187E-5	0.2652403720958164	0.0
86-87	4.5363313154939583E-4	0.0028082051000676888	1.0800788846414187E-5	0.2653375791954341	0.0
88	4.860354980886384E-4	0.002959416143917487	1.0800788846414187E-5	0.26536458116755013	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12980	0.0	52.28819	1
GTATCAA	24020	0.0	39.252926	1
TCAACGC	29475	0.0	31.329735	4
ATCAACG	29780	0.0	30.995064	3
TATCAAC	30225	0.0	30.823868	2
CAACGCA	30025	0.0	30.723314	5
AACGCAG	30795	0.0	29.966995	6
ACGCAGA	35470	0.0	26.07803	7
CGCAGAG	36935	0.0	25.020334	8
GTACATG	31625	0.0	22.986998	1
GCAGAGT	41455	0.0	22.229914	9
TACATGG	33150	0.0	21.656548	2
ACATGGG	33735	0.0	20.948883	3
GAGTACT	22860	0.0	20.457733	12-13
CAGAGTA	40495	0.0	19.586475	10-11
CATGGGG	25970	0.0	19.380243	4
AGAGTAC	37395	0.0	19.234318	10-11
GTACTTT	25625	0.0	18.233925	14-15
GTGGTAT	6165	0.0	18.103579	1
AGTACTT	25655	0.0	17.129002	12-13
>>END_MODULE