FastQCFastQC Report
Fri 27 May 2016
SRR2138933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7379326
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90300.051.763391
GTATCAA167300.039.608431
ATCAACG204550.031.7017143
TCAACGC208500.031.162584
CAACGCA209450.030.9962985
TATCAAC215700.030.2822782
AACGCAG220600.029.5443576
ACGCAGA247450.026.3385947
CGCAGAG252250.025.871498
GCAGAGT293300.022.1332789
CTTATAC66250.022.003651
GTACATG226750.021.7139641
TACATGG225200.021.4433332
ACATGGG222300.020.6785073
CATGGGG167700.019.8464534
TATACAC88650.019.4997123
GAGTACT183100.019.14769412-13
CAGAGTA289250.019.1064310-11
AGAGTAC271400.018.9532510-11
GTACTTT200550.017.53564614-15
Produced by FastQC (version 0.11.5)