##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7379326
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73813394339808	33.0	33.0	33.0	33.0	33.0
2	32.731810466159104	33.0	33.0	33.0	33.0	33.0
3	32.73091079049767	33.0	33.0	33.0	33.0	33.0
4	36.66316015852939	37.0	37.0	37.0	37.0	37.0
5	36.68162458197402	37.0	37.0	37.0	37.0	37.0
6	36.6005503754679	37.0	37.0	37.0	37.0	37.0
7	36.65073924095507	37.0	37.0	37.0	37.0	37.0
8	36.65658936873096	37.0	37.0	37.0	37.0	37.0
9	36.66519706542305	37.0	37.0	37.0	37.0	37.0
10-11	36.62792055805639	37.0	37.0	37.0	37.0	37.0
12-13	36.60233610766078	37.0	37.0	37.0	37.0	37.0
14-15	38.74481151801669	40.0	37.0	40.0	37.0	40.0
16-17	38.69355344919035	40.0	37.0	40.0	37.0	40.0
18-19	38.72103001005783	40.0	37.0	40.0	37.0	40.0
20-21	38.745323068813136	40.0	37.0	40.0	37.0	40.0
22-23	38.57729326721569	40.0	37.0	40.0	37.0	40.0
24-25	38.74399077474068	40.0	37.0	40.0	37.0	40.0
26-27	38.559327027338114	40.0	37.0	40.0	37.0	40.0
28-29	38.5417198743201	40.0	37.0	40.0	37.0	40.0
30-31	38.52291505754974	40.0	37.0	40.0	37.0	40.0
32-33	38.51242949657002	40.0	37.0	40.0	37.0	40.0
34-35	38.42269894210909	40.0	37.0	40.0	37.0	40.0
36-37	33.431441784917304	40.0	37.0	40.0	8.5	40.0
38-39	37.361287133179644	40.0	37.0	40.0	33.0	40.0
40-41	37.941120873387845	40.0	37.0	40.0	35.0	40.0
42-43	38.14975036027717	40.0	37.0	40.0	37.0	40.0
44-45	38.15235500217215	40.0	37.0	40.0	37.0	40.0
46-47	38.05047185524995	40.0	37.0	40.0	33.0	40.0
48-49	38.03246442322087	40.0	37.0	40.0	33.0	40.0
50-51	37.97997210901062	40.0	37.0	40.0	33.0	40.0
52-53	37.905317852268496	40.0	37.0	40.0	33.0	40.0
54-55	37.82377708807412	40.0	37.0	40.0	33.0	40.0
56-57	37.74419955158386	40.0	37.0	40.0	33.0	40.0
58-59	37.59798359562933	40.0	37.0	40.0	33.0	40.0
60-61	37.404740270305524	38.5	37.0	40.0	33.0	40.0
62-63	37.184228390175356	37.0	37.0	40.0	33.0	40.0
64-65	36.97976490593193	37.0	37.0	40.0	33.0	40.0
66-67	36.71199441510456	37.0	37.0	40.0	33.0	40.0
68-69	36.43808143103482	37.0	37.0	40.0	33.0	40.0
70-71	36.1722273244364	37.0	37.0	38.5	33.0	40.0
72-73	35.90871723741828	37.0	37.0	37.0	33.0	40.0
74-75	35.625809294408555	37.0	35.0	37.0	33.0	40.0
76-77	33.78855574656099	35.0	33.0	37.0	27.0	37.0
78-79	35.24173456282439	37.0	33.0	37.0	33.0	37.0
80-81	35.28046391432545	37.0	33.0	37.0	33.0	37.0
82-83	35.34480931078335	37.0	33.0	37.0	33.0	37.0
84-85	35.20924050935869	37.0	33.0	37.0	33.0	37.0
86-87	35.12908897765428	37.0	33.0	37.0	33.0	37.0
88-89	35.04427362900236	37.0	33.0	37.0	33.0	37.0
90-91	34.90241627113159	37.0	33.0	37.0	33.0	37.0
92-93	34.89240657279279	37.0	33.0	37.0	33.0	37.0
94-95	34.85840424664639	37.0	33.0	37.0	33.0	37.0
96-97	34.83675194172267	37.0	33.0	37.0	33.0	37.0
98-99	34.79891361163435	37.0	33.0	37.0	33.0	37.0
100	34.741382115573074	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	33.0
21	173.0
22	784.0
23	2249.0
24	5413.0
25	11107.0
26	19518.0
27	31866.0
28	47184.0
29	66854.0
30	91548.0
31	123085.0
32	164790.0
33	223529.0
34	317470.0
35	498107.0
36	985146.0
37	2353101.0
38	2361661.0
39	75704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5923622292876	20.199581913036504	14.07022809400208	24.137827763673812
2	14.47987255204608	20.941018190550196	41.9552137959483	22.623895461455422
3	17.691158933217693	26.730526344319166	30.799422701647877	24.778892020815267
4	12.13438338224011	18.3606220546322	39.41952663227132	30.085467930856375
5	13.083816778209323	37.952674760477606	35.74783815233209	13.215670308980975
6	29.207166074516834	38.430528750186674	19.128454278886718	13.233850896409779
7	25.882417987767447	32.986129085501844	23.5466491113145	17.584803815416205
8	24.29171715682435	36.38095132265467	21.544515041075567	17.78281647944541
9	25.716454321167	16.661033812573127	21.241208207904084	36.38130365835579
10-11	23.92558615786862	26.653308174757424	29.24605851537119	20.17504715200277
12-13	25.09368064237845	24.284765302413795	28.996042456994044	21.625511598213713
14-15	22.202617149587915	25.517540490825315	26.77384763865968	25.505994720927088
16-17	21.76276668085947	28.58086903871709	27.382216207821692	22.27414807260175
18-19	21.679242792634447	27.80850175205703	29.087548103986734	21.424707351321786
20-21	22.597043302914415	27.020617466981427	28.947444024945863	21.4348952051583
22-23	22.427229228708836	27.064152607539683	28.607300075704206	21.901318088047272
24-25	22.066511884054275	27.22280875274935	28.80466554499198	21.906013818204393
26-27	22.052644280958916	27.487636586819814	28.65572984332545	21.803989288895817
28-29	21.95501051307512	27.489894641212683	28.737476084204772	21.817618761507422
30-31	22.397148559286894	27.233309913177894	28.601986513658	21.767555013877207
32-33	22.057744551409012	27.42447096997737	28.613572108902684	21.904212369710933
34-35	22.21636972445492	27.376584403785582	28.625555990124507	21.78148988163499
36-37	22.29531945591194	27.407748822578203	28.376721920659293	21.920209800850564
38-39	21.98622319239058	27.379136926463644	28.670848731682298	21.963791149463482
40-41	22.33759678021676	27.253520744359733	28.560317012883136	21.848565462540375
42-43	22.252927982313924	27.2793675360714	28.401485412723094	22.066219068891577
44-45	22.20059164426616	27.567955924273146	28.151412039360075	22.08004039210062
46-47	22.360010974869954	27.387097144100547	27.751317949310312	22.501573931719193
48-49	22.330054040244622	27.43053539500544	27.890889167353738	22.3485213973962
50-51	22.179113208658386	27.617977124049766	27.784247669140232	22.41866199815162
52-53	22.35729740755869	27.65137207237853	27.679120100080734	22.31221041998205
54-55	22.348045502923853	27.582775531887048	27.64860747384566	22.420571491343438
56-57	22.45639349773155	27.708313156630265	27.443916200569834	22.391377145068347
58-59	22.279543820928506	27.801026878049107	27.744966359343	22.174462941679387
60-61	22.422491022074716	27.930656143984645	27.425731951538424	22.221120882402214
62-63	22.34874013574534	28.004935552960692	27.36171785691378	22.28460645438019
64-65	22.251450992004738	27.948119632810187	27.37634734971868	22.42408202546639
66-67	22.37924140254677	27.870864699227038	27.239008669817398	22.510885228408792
68-69	22.431716653172735	27.784300409318856	27.39681620482095	22.387166732687454
70-71	22.40636815219862	27.51734833502414	27.383854084320173	22.692429428457064
72-73	22.392478958939467	27.614254884024565	27.422288737244223	22.570977419791745
74-75	22.348963048527693	27.78841511489789	27.284283978196516	22.5783378583779
76-77	22.416645576018688	27.664560865512016	27.25428203613739	22.664511522331907
78-79	22.41809074112993	27.69379158856139	27.21604667494461	22.67207099536407
80-81	22.51963402069967	27.700814049085864	27.136711805336727	22.642840124877743
82-83	22.5332341075055	27.669942587578443	27.332134194950658	22.4646891099654
84-85	22.69909159568868	27.63694643860446	27.1777237381993	22.486238227507556
86-87	22.453575645398264	27.74791666785326	27.249431999731165	22.549075687017307
88-89	22.549288980682043	27.636842010528667	27.267111472051912	22.546757536737374
90-91	22.604077897870678	27.734508386978362	27.228054524918548	22.43335919023241
92-93	22.584706701583826	27.65544693212189	27.28945286166673	22.470393504627555
94-95	22.395843549766788	27.772125276856464	27.314161831717325	22.517869341659424
96-97	22.45567207858262	27.85511459965327	27.443291386202834	22.245921935561277
98-99	23.629375774509896	29.71890350439368	27.602414574400974	19.049306146695454
100	22.61169735457978	30.708822686880882	21.407910856813437	25.2715691017259
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1520.0
1	1377.0
2	1912.5
3	2812.0
4	3940.0
5	6537.0
6	8650.5
7	9797.0
8	10192.5
9	9702.5
10	8866.0
11	8214.0
12	9273.0
13	12522.5
14	19513.0
15	28871.5
16	36918.5
17	42088.5
18	43382.5
19	41025.0
20	36817.5
21	32310.0
22	29805.0
23	30891.0
24	34994.5
25	42453.5
26	53750.0
27	66844.0
28	79920.5
29	96097.5
30	112305.0
31	125908.5
32	142022.0
33	159443.5
34	177523.5
35	195792.5
36	215313.5
37	232767.5
38	241512.5
39	248025.5
40	258938.5
41	268034.5
42	273312.0
43	280963.5
44	290007.0
45	298299.5
46	305537.5
47	312204.0
48	311294.0
49	305746.5
50	301626.5
51	292994.0
52	279544.0
53	262630.0
54	246008.5
55	226955.0
56	205055.0
57	183970.5
58	163628.5
59	142088.0
60	116294.0
61	91427.5
62	70112.5
63	52232.5
64	38403.0
65	27416.5
66	19627.5
67	14640.0
68	10753.5
69	7597.5
70	5438.5
71	3879.5
72	2696.5
73	1789.5
74	1114.0
75	721.0
76	483.5
77	307.0
78	216.5
79	155.5
80	113.0
81	77.5
82	60.0
83	40.0
84	22.5
85	18.0
86	17.0
87	13.5
88	9.0
89	5.0
90	2.5
91	2.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0017074730131180003
4	0.013592027239344079
5	0.009269139214069144
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0015993977589726554
26-27	0.03364989588122969
28-29	0.07884777408807297
30-31	0.07647071238902228
32-33	0.03452856581344436
34-35	0.0014500549999734496
36-37	7.35877415907272
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.5758782410598216E-5
56-57	5.756203208363763E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011737916713248737
74-75	0.034598563785793505
76-77	0.004576661883834842
78-79	0.0
80-81	0.0
82-83	0.0032962448587958142
84-85	0.03721714966256451
86-87	0.04105702314622213
88-89	0.012088648996913327
90-91	4.9873976773024024E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	80.0
20-21	375.0
22-23	758.0
24-25	1575.0
26-27	2582.0
28-29	4338.0
30-31	9175.0
32-33	12147.0
34-35	19133.0
36-37	20981.0
38-39	26262.0
40-41	31984.0
42-43	38855.0
44-45	47800.0
46-47	48014.0
48-49	41987.0
50-51	37018.0
52-53	35469.0
54-55	40064.0
56-57	46061.0
58-59	46731.0
60-61	50304.0
62-63	51061.0
64-65	52364.0
66-67	58045.0
68-69	52393.0
70-71	52418.0
72-73	51348.0
74-75	52351.0
76-77	52726.0
78-79	57107.0
80-81	58775.0
82-83	57706.0
84-85	58665.0
86-87	61824.0
88-89	67956.0
90-91	71716.0
92-93	76784.0
94-95	94491.0
96-97	261594.0
98-99	470020.0
100-101	5058289.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.73028223883621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.8725384167002	74.4132272491212
2	7.522676809283063	12.597517048656373
3	2.002980482517886	5.031303633603088
4	0.7359192234592536	2.4647489714091386
5	0.3438624439324132	1.4395849740898474
6	0.1796234206382677	0.902395182404841
7	0.10867437914992148	0.6369535506847255
8	0.06207450598781473	0.41580127249568444
9	0.04141173630589576	0.3120674732003621
>10	0.12716662887253125	1.5580734095688968
>50	0.002399362546459474	0.13262599069525463
>100	6.458589012052002E-4	0.08401756231130932
>500	2.6731705074174283E-5	0.011683681759258619
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.355137311998413E-5	0.0	0.0	0.0
3	0.0	1.355137311998413E-5	0.0	0.0	0.0
4	0.0	2.710274623996826E-5	0.0	0.0	0.0
5	0.0	2.710274623996826E-5	0.0	0.0	0.0
6	0.0	2.710274623996826E-5	0.0	0.0	0.0
7	0.0	2.710274623996826E-5	0.0	2.710274623996826E-5	0.0
8	0.0	2.710274623996826E-5	0.0	4.065411935995238E-5	0.0
9	0.0	4.065411935995238E-5	0.0	1.2196235807985716E-4	0.0
10-11	0.0	4.065411935995238E-5	0.0	1.2196235807985716E-4	0.0
12-13	0.0	5.420549247993652E-5	0.0	1.2196235807985716E-4	0.0
14-15	0.0	6.0981179039928585E-5	0.0	1.5584079087981747E-4	0.0
16-17	0.0	6.775686559992065E-5	0.0	1.6261647743980953E-4	0.0
18-19	0.0	6.775686559992065E-5	0.0	1.6261647743980953E-4	0.0
20-21	0.0	6.775686559992065E-5	0.0	1.6939216399980162E-4	0.0
22-23	0.0	8.130823871990478E-5	0.0	1.9649491023976986E-4	0.0
24-25	0.0	1.0163529839988096E-4	0.0	2.1682196991974607E-4	0.0
26-27	0.0	1.0841098495987304E-4	0.0	2.642517758396905E-4	0.0
28-29	0.0	1.0841098495987304E-4	0.0	3.794384473595556E-4	0.0
30-31	0.0	1.2196235807985716E-4	0.0	0.00105023141679877	0.0
32-33	0.0	1.2196235807985716E-4	0.0	0.0027034989374368334	0.0
34-35	0.0	1.2196235807985716E-4	0.0	0.00485816726351431	0.0
36-37	0.0	1.2196235807985716E-4	0.0	0.008767738408629731	0.0
38-39	0.0	1.355137311998413E-4	0.0	0.020076359277256487	0.0
40-41	0.0	1.355137311998413E-4	0.0	0.03202189468252249	0.0
42-43	0.0	1.355137311998413E-4	0.0	0.039719074614673486	0.0
44-45	0.0	1.6939216399980162E-4	0.0	0.04938120364922216	0.0
46-47	0.0	2.0327059679976192E-4	0.0	0.059761555459130006	0.0
48-49	0.0	2.0327059679976192E-4	0.0	0.07088723279063697	0.0
50-51	0.0	2.303733430397302E-4	0.0	0.08185029364470414	0.0
52-53	0.0	2.303733430397302E-4	0.0	0.11194789334418889	0.0
54-55	0.0	2.3714902959972225E-4	0.0	0.1489160392155056	0.0
56-57	0.0	2.5747608927969846E-4	0.0	0.18700217336922098	0.0
58-59	0.0	2.642517758396905E-4	0.0	0.2124177736557512	0.0
60-61	0.0	2.710274623996826E-4	0.0	0.23059016500964993	0.0
62-63	0.0	2.710274623996826E-4	0.0	0.24393826753283432	0.0
64-65	0.0	3.2523295487961907E-4	0.0	0.2488912944081885	0.0
66-67	0.0	3.2523295487961907E-4	0.0	0.25344455577650316	0.0
68-69	0.0	3.2523295487961907E-4	0.0	0.2579707143985779	0.0
70-71	0.0	3.2523295487961907E-4	0.0	0.2620564533942531	0.0
72-73	0.0	3.2523295487961907E-4	0.0	0.2657898566888087	0.0
74-75	0.0	3.2523295487961907E-4	0.0	0.26830363640256577	0.0
76-77	0.0	3.2523295487961907E-4	0.0	0.2708309674894428	0.0
78-79	0.0	3.2523295487961907E-4	1.355137311998413E-5	0.27255876756224073	0.0
80-81	0.0	3.3878432799960324E-4	1.355137311998413E-5	0.273209233472	0.0
82-83	0.0	3.3878432799960324E-4	1.355137311998413E-5	0.2736428774118395	0.0
84-85	0.0	3.3878432799960324E-4	1.355137311998413E-5	0.2738325966355193	0.0
86-87	0.0	3.3878432799960324E-4	1.355137311998413E-5	0.27390712918767923	0.0
88	0.0	3.658870742395715E-4	1.355137311998413E-5	0.2739274562473592	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9030	0.0	51.76339	1
GTATCAA	16730	0.0	39.60843	1
ATCAACG	20455	0.0	31.701714	3
TCAACGC	20850	0.0	31.16258	4
CAACGCA	20945	0.0	30.996298	5
TATCAAC	21570	0.0	30.282278	2
AACGCAG	22060	0.0	29.544357	6
ACGCAGA	24745	0.0	26.338594	7
CGCAGAG	25225	0.0	25.87149	8
GCAGAGT	29330	0.0	22.133278	9
CTTATAC	6625	0.0	22.00365	1
GTACATG	22675	0.0	21.713964	1
TACATGG	22520	0.0	21.443333	2
ACATGGG	22230	0.0	20.678507	3
CATGGGG	16770	0.0	19.846453	4
TATACAC	8865	0.0	19.499712	3
GAGTACT	18310	0.0	19.147694	12-13
CAGAGTA	28925	0.0	19.10643	10-11
AGAGTAC	27140	0.0	18.95325	10-11
GTACTTT	20055	0.0	17.535646	14-15
>>END_MODULE